Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A19 F7 I1 R1
|
8 |
41.5 |
1467522 |
91.4% |
1341315 |
145.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913_3_bme_pgi |
3,560,455 |
+G |
intergenic (‑2/+1) |
glpR ← / ← glpR |
pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913_3_bme_pgi | 3,560,455 | 1 | . | G | 97.1%
| 102.0
/ ‑3.8
| 34 | intergenic (‑2/+1) | glpR/glpR | pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base G (19/14); total (20/14) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACC > NC_000913_3_bme_pgi/3560307‑3560581
|
gCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACGTTTCGGGCGCTGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGGT‑GGc > 1:173167/1‑149 (MQ=255)
cGATAAACAGACTGGAGCGAGTGGGGATTTGGTCCGCCACTTTGCGGGCGATGCGCTTTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATc < 2:548393/149‑1 (MQ=255)
gCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGc < 1:33652/149‑1 (MQ=255)
gTCGAGCCATTGGGGATTTGCTCGTCCACTTTGCGCGAGATGCTCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTTTTAACCGAACTGGAAGGCAGCACCGCACCGCCAGGAT‑GGCGCAGCACCAGGTTGTGGTc > 2:190245/1‑149 (MQ=255)
tCGAGCCATTGGGGATTTGCTCCGCAACTTTGAGGGCGATGCGCTCTTTTTATTCGGAATGGGTGGCCTTGGGATAGTGAAACGGCGTGTTAAACGAACTGGAAGGCACCGCCGCAGCGCCATCATAGGG‑CAGACGCAGGTTTTgtcca > 2:318016/1‑145 (MQ=255)
ttGGGGATTTGGGACTGCACTTTGCGGGCGAGGCGCTCTTTTTTTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTc < 2:167675/149‑1 (MQ=255)
ttGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTc > 1:107815/1‑149 (MQ=255)
ttGGGGATTTGCTCCGCCACTTTGCGGGCGATGAGCTCTTTTTCTTCGGTCTGGGTGGGCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCGGCACCGCCATGAT‑GGCGCAGAATCAGGTTTTGCTTCGCCGGCTc > 1:87862/1‑149 (MQ=255)
ggATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCTGTCTGGGTGGCCTTGCGGTCGTGCCACGGCGTGTTAACCGACATGGAATGCAGCGCCGCACCGCCATGAT‑GGCGCAACAATAGGTTTTTCTCGGCCAGCTCAttc > 2:107589/1‑148 (MQ=255)
tttGCCCCGCCACTTTGCGGGCGATGCGCTCTCTTTCTTCGGCCGGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAgg < 1:195279/149‑1 (MQ=255)
cTTTGCGGGCGATGCTCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTACCCGAACTGGAAGGCAGCGCCTCACCGCGATGAT‑GGCGCAGGTTCAGGTTTTGCGCCGCCAGCTCATTGAGGTCGCGGCGAATa < 1:294001/149‑1 (MQ=255)
gcTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGACGGCCGCACCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCCTTGCGGACGCGCCGACTAGTCTACGGGCTGGCg > 2:286606/1‑149 (MQ=255)
gcTCTTTTTCGTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGCCGCGGCGAATAGTCTGCGGGCTGACg > 2:584676/1‑149 (MQ=255)
ctTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGgag < 1:149281/149‑1 (MQ=255)
ttcGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGct < 1:70755/149‑1 (MQ=255)
ttcGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGct > 1:218495/1‑149 (MQ=255)
ttcGGTCTGGGTGGCCTTGCGAGCGTGCCACGGCGTGTTAACCGAAGTGGAAGGCAGCGCCTCACCGCCATGAG‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGct < 2:657760/149‑1 (MQ=255)
tCTGGGTGGCCTTGCGATCGGGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGTTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTAcc < 2:327889/149‑1 (MQ=255)
ctCGTGCCACGGCGTGTTAACCGAACTGGCAGGCAGCGCACCACCGCCATGAT‑GACGCAGCACCAGGTTTCACTCCGCCAGCCCGTTGAGATCGCGGCGAATAGTGTGCGGCCGGACGGAGAAATGCTCTACCAGGCCTTCGGtcttgc > 2:356195/2‑144 (MQ=255)
aTCGTGCAACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGa > 2:638378/1‑149 (MQ=255)
tCGTCCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCAGCGAATAGTCTGCGGGCTGGCGGAGAAGTGCTGTACTGGCCCTTCGGTAGTGAc > 2:556737/1‑149 (MQ=255)
gTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATTAT‑AGCGCAGGATCCGGTAGTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGTCTCACGGAGAAATGCCCTACCAGCTCTTCGGTACTGACAt > 2:83528/1‑149 (MQ=255)
gTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACAt > 1:720571/1‑149 (MQ=255)
ccACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCGCCGCCATGAT‑GGCGCAGGAACAGGTTTTGCTCCGCCAGCTCATTTAGGTGGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCGCTAACAGGTCTTCGAGAGTGACATAAc > 2:69825/1‑149 (MQ=255)
cGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctgct < 1:350146/149‑1 (MQ=255)
gtTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTCCTGACATAACCCTGCGGtt > 1:146460/1‑149 (MQ=255)
ttAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAAGCCTGCTGttt > 1:325001/1‑149 (MQ=255)
ttAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGttt > 2:164865/1‑149 (MQ=255)
tAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTa < 2:107815/149‑1 (MQ=255)
aaCCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTaa < 1:674272/149‑1 (MQ=255)
aCCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAAc > 1:386838/1‑149 (MQ=255)
ccGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCGGCCAGATCATTCAGGTCGCGTCGAATAGTCGGCGGGCTGACGGAGAAATGCTCTAGCAGCTCTTCGTTACTGACATAACCCTGCTAGTTAAcc > 1:152329/1‑149 (MQ=255)
cGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCa < 2:136267/149‑1 (MQ=255)
aGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGataa < 2:720571/149‑1 (MQ=255)
aGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATCATAcc > 2:132858/1‑149 (MQ=255)
|
GCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACC > NC_000913_3_bme_pgi/3560307‑3560581
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
