Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A18 F31 I1 R1	17	84.2	3108850	94.9%	2950298	136.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_pae_tpiA	3,815,809	G→C	intergenic (‑41/+25)	pyrE ← / ← rph	orotate phosphoribosyltransferase/ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_tpiA	3,815,809	0	G	C	84.7%	254.1 / 36.6	87	intergenic (‑41/+25)	pyrE/rph	orotate phosphoribosyltransferase/ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH
Reads supporting (aligned to +/- strand): ref base G (6/7); new base C (31/41); total (38/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

GAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCAC  >  NC_000913_3_pae_tpiA/3815664‑3815952

gaagaaATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCgc                                                                                                                                             >  2:370898/1‑149 (MQ=255)

gaagaaATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTAAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCAAAAAAAAAAAAAAACGCGa                                                                                                                                             <  4:2704/150‑1 (MQ=255)

 aagaaATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAACGCGAc                                                                                                                                            >  4:400375/1‑150 (MQ=255)

  agaaATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAACGCGACt                                                                                                                                           >  3:90723/1‑150 (MQ=255)

     aaTAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAACGCGACtcat                                                                                                                                        <  3:183593/150‑1 (MQ=255)

          gggCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTc                                                                                                                                   >  3:83826/1‑150 (MQ=255)

            gCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTATACAAACAAAAAAAACGCGACTCATCAGTCGc                                                                                                                                 <  2:439384/150‑1 (MQ=255)

              ttttGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCt                                                                                                                               >  1:369150/1‑150 (MQ=255)

                     cccGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAACGCGACTCATCAGTCGCCTTAAAAAt                                                                                                                        <  3:19001/150‑1 (MQ=255)

                       cGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCa                                                                                                                      <  4:359702/150‑1 (MQ=255)

                       cGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAACGCGACTCATCAGTCGCCTTAAAAATCa                                                                                                                      <  3:400375/150‑1 (MQ=255)

                       cGGATTTCAGCGTAAAATCGCCAAACTTTAACACCTACTTGCTAAGCGCAAATTCAATCAACTGGAGATGATATGATTTCATGCCTTCGCTCCTCATATTACTTTTCTACAGACAAAAAAAACGCGACTCATCAGTCGCCTTAAAAATCa                                                                                                                      <  4:159060/150‑1 (MQ=255)

                           tttCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAACGCGACTCATCAGTCGCCTTAAAAATCAGttt                                                                                                                  <  1:655684/150‑1 (MQ=255)

                             tCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGc                                                                                                                <  1:822579/150‑1 (MQ=255)

                               aGCGGAAACTCGCCAAACTTTAACACCTGCATGCTAAGAGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCTCTCCTCATCTTACATTTCAACAAAAAAAAAAAACGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCa                                                                                                              <  2:225890/150‑1 (MQ=255)

                                 cGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAACGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAgc                                                                                                            <  3:35866/150‑1 (MQ=255)

                                  gTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAACGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAgcg                                                                                                           >  4:344108/1‑150 (MQ=255)

                                         cGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAACGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCtt                                                                                                    <  2:755391/150‑1 (MQ=255)

                                           ccAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAACGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCt                                                                                                  <  1:586961/150‑1 (MQ=255)

                                              aaCTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAACGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCg                                                                                               <  4:329638/150‑1 (MQ=255)

                                                 tttAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAACGCGACtcat                                                                                                                                        <  3:211556/106‑1 (MQ=255)

                                                 tttAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAACGCGACtcat                                                                                                                                        >  4:211556/1‑106 (MQ=255)

                                                    aaCACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAACGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTa                                                                                         <  2:713199/150‑1 (MQ=255)

                                                    aaCACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAACGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTa                                                                                         >  3:316121/1‑150 (MQ=255)

                                                    aaCACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAACGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTa                                                                                         <  4:301798/150‑1 (MQ=255)

                                                     acacCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAACGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTAc                                                                                        >  1:223042/1‑150 (MQ=255)

                                                       acCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAACGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACaa                                                                                      >  2:418671/1‑150 (MQ=255)

                                                           gcttgctAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGga                                                                                  <  2:369150/150‑1 (MQ=255)

                                                              tgctAAGCGCAAATTCAATAAACTGCCGCTGATATGGTTTCATGCCTTGCCTCCTCATCTTACTTTTCTACAAACAAAAAAAACGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgattc                                                                               <  4:402845/150‑1 (MQ=255)

                                                               gctAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAACGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgattcg                                                                              <  1:327581/150‑1 (MQ=255)

                                                                ctAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAACGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgattcga                                                                             >  3:39303/1‑150 (MQ=255)

                                                                ctAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAACGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgattcga                                                                             <  3:438053/150‑1 (MQ=255)

                                                                ctAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTACTACAGACAAAAAAAACGCGACTCATCAGTCGCCTCAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgattcga                                                                             <  2:557828/150‑1 (MQ=255)

                                                                   aGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAACGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgattcgattc                                                                          >  4:135823/1‑150 (MQ=255)

                                                                     cgcAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAACGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTccc                                                                        <  2:585035/150‑1 (MQ=255)

                                                                       cAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCATCCTCTTACTCTTCTACCGACAAAACAAACGCGACTCATCAGCCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGAGTCGCTACACTGgattccattacaac                                                                      >  2:805888/1‑145 (MQ=255)

                                                                          aTTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAACGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCgg                                                                   <  2:475295/150‑1 (MQ=255)

                                                                          aTTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAACGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCgg                                                                   >  1:802190/1‑150 (MQ=255)

                                                                          aTTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAACGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCgg                                                                   <  1:787295/150‑1 (MQ=255)

                                                                          aTTCAATAAACTGGCGCTCATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAACGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCgg                                                                   <  3:77897/150‑1 (MQ=255)

                                                                            tCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAACGCGACtcatca                                                                                                                                      >  1:846548/1‑81 (MQ=255)

                                                                            tCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAACGCGACtcatca                                                                                                                                      <  2:846548/81‑1 (MQ=255)

                                                                            tCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAACGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGc                                                                 <  3:499827/150‑1 (MQ=255)

                                                                              aaTAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAACGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCa                                                               >  1:653399/1‑150 (MQ=255)

                                                                              aaTAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAACGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCa                                                               >  3:389274/1‑150 (MQ=255)

                                                                               aTAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCag                                                              >  2:796330/1‑150 (MQ=255)

                                                                               aTAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAACGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCag                                                              >  1:107238/1‑150 (MQ=255)

                                                                                tAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAACGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCaga                                                             <  1:676321/150‑1 (MQ=255)

                                                                                tAAACCGGCGCTGATATGGTTTCATCCCTTCGCGCCTCATTTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCaga                                                             <  1:370898/150‑1 (MQ=255)

                                                                                          cTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAACGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGa                                                   <  1:426356/150‑1 (MQ=255)

                                                                                           tGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAACGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGAt                                                  <  4:303355/150‑1 (MQ=255)

                                                                                           tGATATGGTTTCATGCCTTCGCTCCTCAAATTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGAt                                                  <  2:171255/150‑1 (MQ=255)

                                                                                             atatGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAACGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGa                                                <  3:536174/150‑1 (MQ=255)

                                                                                              tatGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAACGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAg                                               <  2:427191/150‑1 (MQ=255)

                                                                                               atGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGt                                              <  1:294902/150‑1 (MQ=255)

                                                                                                tGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAACGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCaga                                                             <  2:693543/134‑1 (MQ=255)

                                                                                                tGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAACGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCaga                                                             >  1:693543/1‑134 (MQ=255)

                                                                                                 ggTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAACGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACGATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAg                                            >  2:428052/1‑150 (MQ=255)

                                                                                                 ggTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAACGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCag                                                              >  2:585808/1‑132 (MQ=255)

                                                                                                 ggTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAACGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCag                                                              <  1:585808/132‑1 (MQ=255)

                                                                                                   tttCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAACGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGct                                          >  2:865777/1‑150 (MQ=255)

                                                                                                     tCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAACGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGctct                                        <  1:418671/150‑1 (MQ=255)

                                                                                                           cTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATgg                                  <  4:83826/150‑1 (MQ=255)

                                                                                                           cTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAACGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATgg                                  <  1:820679/150‑1 (MQ=255)

                                                                                                             tCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAACGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGt                                <  2:107238/150‑1 (MQ=255)

                                                                                                                ctcctcATCTTACTTTTCTACAGACAAAAAAAACGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCAAACAAGATGAGTAGCTCTTCATGGGTGaa                             <  4:120666/150‑1 (MQ=255)

                                                                                                                     cATCTTACTTTTCTACAGACAAAAAAAACGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGt                                >  1:541273/1‑142 (MQ=255)

                                                                                                                     cATCTTACTTTTCTACAGACAAAAAAAACGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGt                                <  2:541273/142‑1 (MQ=255)

                                                                                                                      aTCTTACTTTTCTACAGACAAAAAAAACGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTc                       >  1:340647/1‑150 (MQ=255)

                                                                                                                      aTCTTACTTTTCTACAGACAAAAAAAACGCGACTCATCAGTCGCCTTAAAAAGCAGTTTGCCAGTGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTc                       <  4:569902/150‑1 (MQ=255)

                                                                                                                             ttttCTACAGACAAAAAAAACGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCg                                                                                               >  4:160267/1‑71 (MQ=255)

                                                                                                                             ttttCTACAGACAAAAAAAACGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCg                                                                                               <  3:160267/71‑1 (MQ=255)

                                                                                                                             ttttCTACAGACAAAAAAAACGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCt                >  4:504065/1‑150 (MQ=255)

                                                                                                                             ttttCTACAGACAAAAAAAACGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCt                >  3:563136/1‑150 (MQ=255)

                                                                                                                               ttCTACAGACAAAAAAAACGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGc              >  2:226038/1‑150 (MQ=255)

                                                                                                                                tCTACAGACAAAAAAAACGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGcc             <  4:389274/150‑1 (MQ=255)

                                                                                                                                 cTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCg            >  2:105560/1‑150 (MQ=255)

                                                                                                                                  tACAGACAAAAAAAACGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGt           >  4:276969/1‑150 (MQ=255)

                                                                                                                                   aCAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTc          >  2:351893/1‑150 (MQ=255)

                                                                                                                                   aCAGACAAAAAAAACGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTc          >  2:757562/1‑150 (MQ=255)

                                                                                                                                     aGACAAAAAAAACGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTccc        >  1:49078/1‑150 (MQ=255)

                                                                                                                                       aCAAAAAAAACGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCt      >  1:243022/1‑150 (MQ=255)

                                                                                                                                         aaaaaaaaTGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGa                                                   >  2:262572/1‑103 (MQ=255)

                                                                                                                                         aaaaaaaaTGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGa                                                   <  1:262572/103‑1 (MQ=255)

                                                                                                                                         aaaaaaaaCGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGc    >  2:482667/1‑150 (MQ=255)

                                                                                                                                          aaaaaaaCGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCa   <  4:90723/150‑1 (MQ=255)

                                                                                                                                           aaaaaaCGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCAc  >  4:525454/1‑150 (MQ=255)

GAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCAC  >  NC_000913_3_pae_tpiA/3815664‑3815952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A