Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F1 I2 R1	67	32.5	1435938	95.9%	1377064	112.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,408,553	G→A	A331A (GCG→GCA)	alaA →	glutamate‑pyruvate aminotransferase, glutamic‑pyruvic transaminase (GPT), alanine transaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,408,553	0	G	A	100.0%	30.1 / NA	10	A331A (GCG→GCA)	alaA	glutamate‑pyruvate aminotransferase, glutamic‑pyruvic transaminase (GPT), alanine transaminase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (5/5); total (5/5)

GACCGCGCTAGGTGGTTATCAGAGCATCAGTGAATTTATTACCCCTGGCGGTCGTCTTTATGAGCAGCGTAACCGCGCGTGGGAACTGATCAACGATATTCCGGGCGTTTCCTGCGTGAAACCTCGTGGTGCGCTGTATATGTTCCCGAAAATCGACGCCAAACGCTTTAACATTCACGACGATCAGAAAATGGTGTTGGATTTCCTGTTGCAGGAAAAAGTTCTGTTGGTGCAAGGGACGGCATTCAACTGGCCGTGGCCGGA  >  NC_000913/2408421‑2408684

gACCGCGCTAGGTGGTTATCAGAGCATCAGTGAATTTATTACCCCTGGCGGTCGTCTTTATGAGCAGCGTAACCGCGCGTGGGAACTGATCAACGATATTCCGGGCGTTTCCTGTGTGAAACCTCGTGGTGCACTGtat                                                                                                                               >  1:399258/1‑139 (MQ=255)

                        cATCAGTGAATTTATTACCCCTGGCGGTCGTCTTTATGAGCAGCGTAACCGCGCGTGGGAACTGATCAACGATATTCCGGGCGTGTCCTGCGTGAAACCTCGTGGTGCACTGTATATGTTCCCGAAAATCGACGCCaaa                                                                                                       <  2:21870/139‑1 (MQ=255)

                                      ttaCCCCTGGCGGTCGTCTTTATGAGCAGCGTAACCGCGCGTGGGAACTGATCAACGATATTCCGGGCGTTTCCTGCGTGAAACCTCGTGGTGCACTGTATATGTTCCCGAAAATCGACGCCAAACGCTTTAACATTCa                                                                                         <  2:399258/139‑1 (MQ=255)

                                              ggCGGTCGTCTTTATGAGCAGCGTAACCGCGCGTGGGAACTGATCAACGATATTCCGGGCGTTTCCTGCGTGAAACCTCGTGGTGCACTGTATATGTTCCCGAAAATCGACGCCAAACGCTTTAACATTCACGACGATc                                                                                 >  2:547973/1‑139 (MQ=255)

                                                                            gcgTGGGAACTGATCAACGATATTCCGGGCGTTTCCTGCGTGAAACCTCGTGGTGCACTGTATATGTTCCCGAAAATCGACGCCa                                                                                                         <  1:552091/85‑1 (MQ=255)

                                                                            gcgTGGGAACTGATCAACGATATTCCGGGCGTTTCCTGCGTGAAACCTCGTGGTGCACTGTATATGTTCCCGAAAATCGACGCCa                                                                                                         >  2:552091/1‑85 (MQ=255)

                                                                                                                      aaaCCTCGTGGTGCACTGTATATGTTCCCGAAAATCGACGCCAAACGCTTTAACATTCACGACGATCAGAAAATGGTGTTGGATTTCCTGTTGCAGGAAAAAGTTCTGTTGGTGCAAGGGACGGCATTCAACtggccgt         >  1:680557/1‑139 (MQ=255)

                                                                                                                             gtggtgCACTGTATATGTTCCCGAAAATCGACGCCAAACGCTTTAACATTCAcgacga                                                                                   <  1:659231/58‑1 (MQ=255)

                                                                                                                             gtggtgCACTGTATATGTTCCCGAAAATCGACGCCAAACGCTTTAACATTCAcgacga                                                                                   >  2:659231/1‑58 (MQ=255)

                                                                                                                             gtggtgCACTGTATATGTTCCCGAAAATCGACGCCAAACGCTTTAACATTCACGACGATCAGAAAATGGTGTTGGATTTCCTGTTGCAGGAAAAAGTTCTGTTGGTGCAAGGGACGGCATTCAACTGGCCGTGGCCGGa  <  2:680557/139‑1 (MQ=255)

GACCGCGCTAGGTGGTTATCAGAGCATCAGTGAATTTATTACCCCTGGCGGTCGTCTTTATGAGCAGCGTAACCGCGCGTGGGAACTGATCAACGATATTCCGGGCGTTTCCTGCGTGAAACCTCGTGGTGCGCTGTATATGTTCCCGAAAATCGACGCCAAACGCTTTAACATTCACGACGATCAGAAAATGGTGTTGGATTTCCTGTTGCAGGAAAAAGTTCTGTTGGTGCAAGGGACGGCATTCAACTGGCCGTGGCCGGA  >  NC_000913/2408421‑2408684

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A