Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A4 F1 I2 R1
|
67 |
32.5 |
1435938 |
95.9% |
1377064 |
112.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,972,909 |
(C)6→7 |
coding (954/1194 nt) |
lplT ← |
lysophospholipid transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,972,903 | 1 | . | C | 95.8%
| 82.3
/ ‑2.6
| 24 | coding (960/1194 nt) | lplT | lysophospholipid transporter |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (12/11); total (13/11) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.46e-01 |
GCGAGTAAATGCCCAGCATCAACAACATGGCGCTGTTTTCGCCAAGGTTTTGTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTAGCAACGCATTGAGCGGAACGACAAAAAAG‑CCCCCCATCACGCCAATCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAAAAAATCAGTACCACCACGCCAATCAAAATCCCGGCTGGCATACAGCGTGACACGGTTTCCAGC > NC_000913/2972773‑2973029
|
gCGAGTAAATGCCCAGCATCAACAACATGGCGCTGTTTTCGCCAAGGTTTTGTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTAGCAACGCATTGAGCGGAACGACAAAAAAG‑ccccccca < 2:712957/139‑3 (MQ=255)
cGAGTAAATGCCCAGCATCAACAACATGGCGCTGTTTTCGCCAAGGTTTTGTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTAGCAACGCATTGAGCGGAACGACAAAAAAG‑cccccccat > 2:566696/1‑136 (MQ=255)
aTGCCCAGCATCAACAACATGGCGCTGTTTTCGCCAAGGTTTTGTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTAGCAACGCATTGAGCGGAACGACAAAAAAGCCCCCCCATCACGCCa < 2:678348/139‑1 (MQ=255)
cATCAACAACATGGCGCTGTTTTCGCCAAGGTTTTGTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTAGCAACGCATTGAGCGGAACGACAAAAAAGCCCCCCCATCACGCCAATCAGCAt < 2:116469/139‑1 (MQ=255)
aTGGCGCTGTTTTCGCCAAGGTTTTGTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTAGCAACGCATTGAGCGGAACGACAAAAAAGCCCCCCCATCACGCCAATCAGCATCAACAAGGCa > 2:52954/1‑139 (MQ=255)
cgcTGTTTTCGCCAAGGTTTTGTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTAGCAACGCATTGAGCGGAACGACAAAAAAGCCCCCCCATCACGCCaa > 1:639154/1‑118 (MQ=255)
cgcTGTTTTCGCCAAGGTTTTGTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTAGCAACGCATTGAGCGGAACGACAAAAAAGCCCCCCCATCACGCCaa < 2:639154/118‑1 (MQ=255)
ggTTTTGTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTAGCAACGCATTGAGCGGAACGACAAAAAAGCCCCCCCATCACGCCAATCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCgt > 1:529611/1‑139 (MQ=255)
ggTTTTGTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTAGCAACGCATTGAGCGGAACGACAAAAAAGCCCCCCCATCACGCCAATCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCgt > 1:384803/1‑139 (MQ=255)
gTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTAGCAACGCATTGAGCGGAACGACAAAAAAGCCCCCCCATCACGCCAATCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCa < 1:52954/139‑1 (MQ=255)
gCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTAGCAACGCATTGTGCGGAACGACAAAAAAGCCCCCCCATCACGCCAATCAGCATCAACAAGGCATAGGCTGGCAGCAGCACGTGGCGCAGGGaa > 2:486886/1‑139 (MQ=255)
cAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTAGCAACGCATTGAGCGGAACGACAAAAAAGCCCCCCCATCACGCCAATCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaa > 2:616665/1‑139 (MQ=255)
cgccccgACGCTTTTTTTACCCCGCTCCTGTAGCAACGCATTGAGCGGAACGACAAAAAAGCCCCCCCATCACGCCAATCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAAAAAATCAGTaccac > 1:69918/1‑139 (MQ=255)
ccgACGCTTTTTTTACCCCGCTCCTGTAGCAACGCATTGAGCGGAACGACAAAAAAGCCCCCCCATCACGCCAATCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAAAAAATCAGTACCACCACg < 1:566696/139‑1 (MQ=255)
aCGCTTTTTTTACCCCGCTCCTGTAGCAACGCATTGAGCGGAACGACAAAAAAGCCCCCCCATCACGCCAATCAGCATCTACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAAAAAATCAGTACCACCACGCCa > 2:433654/1‑139 (MQ=255)
cccGCTCCTGTAGCAACGCATTGAGCGGAACGACAAAAAAGCCCCCCCATCACGCCAATCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAAAAAATCAGTACCACCACGCCAATCAAAATCCCgg < 2:529611/139‑1 (MQ=255)
gTAGCAACGCATTGAGCGGAACGACAAAAAAG‑CCCCCCATCACGCCAATCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAAAAAATCAGTACCACCACGCCAATCAAAATCCCGGCTGGCATACa > 1:41752/1‑139 (MQ=255)
aCGCATTGAGCGGAACGACAAAAAAGCCCCCCCATCACGCCAATCAg < 2:211441/47‑1 (MQ=255)
aCGCATTGAGCGGAACGACAAAAAAGCCCCCCCATCACGCCAATCAg > 1:211441/1‑47 (MQ=255)
cGCATTGAGCGGAACGACAAAAAAGCCCCCCCATCACGCCAATCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAAAAAATCAGTACCACCACGCCAATCa > 1:663583/1‑114 (MQ=255)
cGCATTGAGCGGAACGACAAAAAAGCCCCCCCATCACGCCAATCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAAAAAATCAGTACCACCACGCCAATCa < 2:663583/114‑1 (MQ=255)
ttGAGCGGAACGACAAAAAAGCCCCCCCATCACGCCAATCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTg > 1:111863/1‑78 (MQ=255)
ttGAGCGGAACGACAAAAAAGCCCCCCCATCACGCCAATCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTg < 2:111863/78‑1 (MQ=255)
tGAGCGGAACGACAAAAAAGCCCCCCCATCACGCCAATCAGCATcaaca > 1:516250/1‑49 (MQ=255)
tGAGCGGAACGACAAAAAAGCCCCCCCATCACGCCAATCAGCATcaaca < 2:516250/49‑1 (MQ=38)
aCGACAAAAAAGCCCCCCCATCACGCCAATCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAAAAAATCAGTACCACCACGCCAATCAAAATCCCGGCTGGCATACAGCGTGACACGGTTTCCAGc < 1:616665/139‑1 (MQ=255)
|
GCGAGTAAATGCCCAGCATCAACAACATGGCGCTGTTTTCGCCAAGGTTTTGTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTAGCAACGCATTGAGCGGAACGACAAAAAAG‑CCCCCCATCACGCCAATCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAAAAAATCAGTACCACCACGCCAATCAAAATCCCGGCTGGCATACAGCGTGACACGGTTTCCAGC > NC_000913/2972773‑2973029
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
