Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F1 I2 R1	92	50.6	2362856	94.3%	2228173	111.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	632,923	A→G	A185A (GCT→GCC)	ybdH ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	632,923	0	A	G	100.0%	213.8 / NA	57	A185A (GCT→GCC)	ybdH	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (29/28); total (29/28)

ATTGCGTTAACTGTTGATTTTGCTGATCGCTCAGCGCCTGTTCGCTACTGTTTAACAAGACGTCGCGAATGGCTTGCGCATTATTGATCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAA  >  NC_000913/632796‑633047

aTTGCGTTAACTGTTGATTTTGCTGATCGCTCAGCGCCTGTTCGCTACTGTTTAACAAGACGTCGCGAATGGCTTGCGCATTATTGATCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGGGCCAGcaccac                                                                                                                   <  1:852983/139‑1 (MQ=255)

         aCTGTTGATTTTGCTGATCGCTCAGCGCCTGTTCGCTACTGTTTAACAAGACGTCGCGAATGGCTTGCGCATTATTGATCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGGGCCAGCACCACCGCTTCATa                                                                                                          >  2:164605/1‑139 (MQ=255)

                    tGCTGATCGCTCAGCGCCTGTTCGCTACTGTTTAACAAGACGTCGCGAATGGCTTGCGCATTATTGATCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGGGCCAGCACCACCGCTTCATACCATTTCGCCa                                                                                               >  1:1040794/1‑139 (MQ=255)

                          tCGCTCAGCGCCTGTTCGCTACTGTTTAACAAGACGTCGCGAATGGCTTGCGCATTATTGATCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGGGCCAGCACCACCGCTTCATACCATTTCGCCAGCg                                                                                            >  2:320742/1‑136 (MQ=255)

                          tCGCTCAGCGCCTGTTCGCTACTGTTTAACAAGACGTCGCGAATGGCTTGCGCATTATTGATCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGGGCCAGCACCACCGCTTCATACCATTTCGCCAGCg                                                                                            <  1:320742/136‑1 (MQ=255)

                                       gTTCGCTACTGTTTAACAAGACGTCGCGAATGGCTTGCGCATTATTGATCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGGGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCCGc                                                                            >  2:969954/1‑139 (MQ=255)

                                           gCTACTGTTTAACAAGACGTCGCGAATGGCTTGCGCATTATTGATCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGGGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCCGCcagc                                                                        <  2:516169/139‑1 (MQ=255)

                                                  tttAACAAGACGTCGCGAATGGCTTGCGCATTATTGATCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGGGCCAGcaccac                                                                                                                   <  2:1013469/89‑1 (MQ=255)

                                                  tttAACAAGACGTCGCGAATGGCTTGCGCATTATTGATCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGGGCCAGcaccac                                                                                                                   >  1:1013469/1‑89 (MQ=255)

                                                    tAACAAGACGTCGCGAATGGCTTGCGCATTATTGATCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGGGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCCGCCAGCAGATAttgt                                                               >  2:654687/1‑139 (MQ=255)

                                                      atataatgttgcgAATGGCTTGCGCATTTATGAACCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGGGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCCGCCAGCAGATAttgttg                                                             <  1:434986/129‑1 (MQ=255)

                                                      aCAAGACGTCGCGAATGGCTTGCGCATTATTGATCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGGGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCCGCCAGCAGATAttgttg                                                             <  1:969954/139‑1 (MQ=255)

                                                            cGTCGCGAATGGCTTGCGCATTATTGATCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGGGCCAGcacca                                                                                                                    >  1:231410/1‑78 (MQ=255)

                                                            cGTCGCGAATGGCTTGCGCATTATTGATCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGGGCCAGcacca                                                                                                                    <  2:231410/78‑1 (MQ=255)

                                                            cGTCGCGAATGGCTTGCGCATTATTGATCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGGGCCAGCACCACCGCTTCa                                                                                                            >  2:645587/1‑86 (MQ=255)

                                                            cGTCGCGAATGGCTTGCGCATTATTGATCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGGGCCAGCACCACCGCTTCa                                                                                                            <  1:645587/86‑1 (MQ=255)

                                                            cGTCGCGAATGGCTTGCGCATTATTGATCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGGGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGc                                                       >  2:27616/1‑139 (MQ=255)

                                                            cGTCGCGAATGGCTTGCGCATTATTCATCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGGGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGc                                                       >  1:1129220/1‑139 (MQ=255)

                                                             gTCGCGAATGGCTTGCGCATTATTGATCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGGGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGCa                                                      <  2:946717/139‑1 (MQ=255)

                                                               cgcgAATGGCTTGCGCATTATTGATCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGGGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCCg                                                                             <  2:1298/114‑1 (MQ=255)

                                                               cgcgAATGGCTTGCGCATTATTGATCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGGGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCCg                                                                             >  1:1298/1‑114 (MQ=255)

                                                               cgcgAATGGCTTGCGCATTATTGATCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGGGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGCAtt                                                    >  1:1098173/1‑139 (MQ=255)

                                                               cgcgAATGGCTTGCGCATTATTGATCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGGGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGCAtt                                                    >  1:38119/1‑139 (MQ=255)

                                                                    aTGGCTTGCGCATTATTGATCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGGGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGCATTGAGGa                                               <  2:1098173/139‑1 (MQ=255)

                                                                     tGGCTTGCGCATTATTGATCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGGGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGt                                                         >  1:621233/1‑128 (MQ=255)

                                                                     tGGCTTGCGCATTATTGATCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGGGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGt                                                         <  2:621233/128‑1 (MQ=255)

                                                                                      aTCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGGGCCAGCACCACCGCTTCATAc                                                                                                         >  1:1049084/1‑63 (MQ=255)

                                                                                      aTCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGGGCCAGCACCACCGCTTCATAc                                                                                                         <  2:1049084/63‑1 (MQ=255)

                                                                                      aTCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGGGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCCGCca                                                                          >  1:65896/1‑94 (MQ=255)

                                                                                      aTCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGGGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCCGCca                                                                          <  2:65896/94‑1 (MQ=255)

                                                                                      aTCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGGGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGCATTGAGGATa                                             <  1:1018351/123‑1 (MQ=255)

                                                                                      aTCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGGGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGCATTGAGGATa                                             >  2:1018351/1‑123 (MQ=255)

                                                                                       tCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGGGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAg                            <  1:27616/139‑1 (MQ=255)

                                                                                          ccAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGGGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCAc                         <  2:1114671/139‑1 (MQ=255)

                                                                                           cAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGGGCCAGCACCACCGCTTCATACCATTTCGCCAGCg                                                                                            <  1:1131854/71‑1 (MQ=255)

                                                                                           cAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGGGCCAGCACCACCGCTTCATACCATTTCGCCAGCg                                                                                            >  2:1131854/1‑71 (MQ=255)

                                                                                           cAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGGGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCCGCCAGCAGATAttgtt                                                              <  2:473046/101‑1 (MQ=255)

                                                                                           cAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGGGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCCGCCAGCAGATAttgtt                                                              >  1:473046/1‑101 (MQ=255)

                                                                                           cAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGGGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGt                                    <  1:173923/127‑1 (MQ=255)

                                                                                           cAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGGGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGt                                    >  2:173923/1‑127 (MQ=255)

                                                                                           cAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGGGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCAcc                        >  2:116894/1‑139 (MQ=255)

                                                                                             gTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGGGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCCGCca                                                                          <  2:943132/87‑1 (MQ=255)

                                                                                             gTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGGGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCCGCca                                                                          >  1:943132/1‑87 (MQ=255)

                                                                                             gTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGGGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGCa                                                      >  1:396861/1‑107 (MQ=255)

                                                                                             gTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGGGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGCa                                                      <  2:396861/107‑1 (MQ=255)

                                                                                                 cACGGTTAGCGGCAACGTTTCTGGTTGCGGGGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTccacc                              <  1:701292/127‑1 (MQ=255)

                                                                                                 cACGGTTAGCGGCAACGTTTCTGGTTGCGGGGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTccacc                              >  2:701292/1‑127 (MQ=255)

                                                                                                 cACGGTTAGCGGCAACGTTTCTGGTTGCGGGGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATaaaa                  >  2:1013979/1‑139 (MQ=255)

                                                                                                                cGTTTCTGGTTGCGGGGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAg                            >  1:854621/1‑114 (MQ=255)

                                                                                                                cGTTTCTGGTTGCGGGGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAg                            <  2:854621/114‑1 (MQ=255)

                                                                                                                cGTTTCTGGTTGCGGGGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGaaa   >  1:366600/1‑139 (MQ=255)

                                                                                                                           gCGGGGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGCATTGAGGATAAtct                                         <  1:549578/90‑1 (MQ=255)

                                                                                                                           gCGGGGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGCATTGAGGATAAtct                                         >  2:549578/1‑90 (MQ=255)

                                                                                                                            cGGGGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCCGCcagc                                                                        <  1:132946/58‑1 (MQ=255)

                                                                                                                            cGGGGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCCGCca                                                                          >  2:184580/1‑56 (MQ=255)

                                                                                                                            cGGGGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCCGCca                                                                          <  1:184580/56‑1 (MQ=255)

                                                                                                                            cGGGGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGaaaa  <  2:619863/128‑1 (MQ=255)

ATTGCGTTAACTGTTGATTTTGCTGATCGCTCAGCGCCTGTTCGCTACTGTTTAACAAGACGTCGCGAATGGCTTGCGCATTATTGATCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAA  >  NC_000913/632796‑633047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A