Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A13 F1 I2 R1
|
92 |
50.6 |
2362856 |
94.3% |
2228173 |
111.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,351,093 |
G→A |
G190G (GGC→GGT) |
dcuS ← |
sensory histidine kinase in two‑component regulatory system with DcuR, regulator of anaerobic fumarate respiration |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,351,093 | 0 | G | A | 100.0%
| 152.1
/ NA
| 43 | G190G (GGC→GGT) | dcuS | sensory histidine kinase in two‑component regulatory system with DcuR, regulator of anaerobic fumarate respiration |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (21/22); total (21/22) |
ATAGACTGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATGCCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTAGATGGGGGTAAATA > NC_000913/4350971‑4351220
|
aTAGACTGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGAcc < 2:369549/139‑1 (MQ=255)
aCTGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGAt > 2:606810/1‑139 (MQ=255)
ggCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGGCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGAc < 2:726276/124‑1 (MQ=255)
ggCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTACAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGAc > 1:726276/1‑124 (MQ=255)
ccTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCg > 1:900803/1‑139 (MQ=255)
ttgCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGAc < 2:10482/137‑1 (MQ=255)
tGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGAc < 1:606810/139‑1 (MQ=255)
gCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCAtt > 2:1126556/1‑139 (MQ=255)
cTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGAc > 2:589370/1‑112 (MQ=255)
cTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGAc < 1:589370/112‑1 (MQ=255)
aGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGtt < 2:270256/139‑1 (MQ=255)
cGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGt < 1:1167189/125‑1 (MQ=255)
cGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGt > 2:1167189/1‑125 (MQ=255)
cGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCa < 2:229724/127‑1 (MQ=255)
cGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCa > 1:229724/1‑127 (MQ=255)
tCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGc > 2:633060/1‑139 (MQ=255)
ccAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAg > 1:406423/1‑139 (MQ=255)
cAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAgg > 1:392509/1‑139 (MQ=255)
aaaaGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGAc < 1:50787/94‑1 (MQ=255)
aaaaGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGAc > 2:50787/1‑94 (MQ=255)
aaaaGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCg > 2:949231/1‑139 (MQ=255)
aGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGcca < 2:1019173/139‑1 (MQ=255)
tACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGt < 1:1126556/139‑1 (MQ=255)
aCCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGtt > 1:864122/1‑139 (MQ=255)
ccTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGttt < 1:422827/139‑1 (MQ=255)
aGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGAtttt < 2:864122/139‑1 (MQ=255)
aaTGCAGGTGCCAATCAGTCCGACCAGCATACCAaataat < 2:1014493/40‑1 (MQ=255)
aaTGCAGGTGCCAATCAGTCCGACCAGCATACCAaataat > 1:1014493/1‑40 (MQ=255)
aaTGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATAc < 1:453002/42‑1 (MQ=255)
aaTGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATAc > 2:453002/1‑42 (MQ=255)
aaTGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTc < 1:1030678/58‑1 (MQ=255)
aaTGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTc > 2:1030678/1‑58 (MQ=255)
aTGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCAt > 1:976771/1‑139 (MQ=255)
gTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGAt > 2:135652/1‑127 (MQ=255)
gTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGAt < 1:135652/127‑1 (MQ=255)
tGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTAGAt < 2:406423/139‑1 (MQ=255)
aaTCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGa < 2:180259/52‑1 (MQ=255)
aaTCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGa > 1:180259/1‑52 (MQ=255)
gTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTAGATGGGGGTaaa < 1:214005/139‑1 (MQ=255)
tCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTAGATGGGGGTAAAt > 1:373891/1‑139 (MQ=255)
ccGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTAGATGGGGGTAAATa < 1:633060/139‑1 (MQ=255)
cATACCAAATAATACCGACCAGATAATGCTCCAGCGAc < 2:306950/38‑1 (MQ=255)
cATACCAAATAATACCGACCAGATAATGCTCCAGCGAc > 1:306950/1‑38 (MQ=255)
|
ATAGACTGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATGCCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTAGATGGGGGTAAATA > NC_000913/4350971‑4351220
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
