Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A13 F1 I1 R1
|
86 |
24.1 |
1002126 |
98.1% |
983085 |
121.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,544,191 |
A→G |
K155R (AAA→AGA) |
fimI → |
fimbrial protein involved in type 1 pilus biosynthesis |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,544,191 | 0 | A | G | 100.0%
| 89.1
/ NA
| 24 | K155R (AAA→AGA) | fimI | fimbrial protein involved in type 1 pilus biosynthesis |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (9/15); total (9/15) |
AGGGGCCAGGGATAGCCACCAATATTGGCGTAGCGTTGTTTGATGATGAAGGAAACCTCGTACCGATTAATCGTCCTCCAGCAAACTGGAAACGGCTTTATTCAGGCTCTACTTCGCTACATTTCATCGCCAAATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCAATGCCCAGGCCTGGTTCTCTTTAACCTATCAGTAATTGTTCAGCAGATAATGTGATAACAGGAACAGGACAGTGAGTAATAAAAACGTCAAT > NC_000913/4544059‑4544324
|
agGGGCCAGGGATAGCCACCAATATTGGCGTAGCGTTGTTTGATGATGAAGGAAACCTCGTACCGATTAATCGTCCTCCAGCAAACTGGAAACGGCTTTATTCAGGCTCTACTTCGCTACATTTCATCGCCAGATATCg > 1:127257/1‑139 (MQ=255)
agGGGCCAGGGATAGCCACCAATATTGGCGTAGCGTTGTTTGATGATGAAGGAAACCTCGTACCGATTAATCGTCCTCCAGCAAACTGGAAACGGCTTTATTCAGGCTCTACTTCGCTACATTTCATCGCCAGATATCg > 2:166066/1‑139 (MQ=255)
gggCCAGGGATAGCCACCAATATTGGCGTAGCGTTGTTTGATGATGAAGGAAACCTCGTACCGATTAATCGTCCTCCAGCAAACTGGATACGGCTTTATTCAGGCTCTACTTCGCTACATTTCATCGCCAGATATCGTg > 2:317775/1‑139 (MQ=255)
gATAGCCACCAATATTGGCGTAGCGTTGTTTGATGATGAAGGAAACCTCGTACCGATTAATCGTCCTCCAGCAAACTGGAAACGCCTTTATTCAGGCTCTACTTCGCTACATTTCATCGCCAGATATCGTGCTACCggg > 1:329457/1‑139 (MQ=255)
ttgttTGATGATGAAGGAAACCTCGTACCGATTAATCGTCCTCCAGCAAACTGGAAACGGCTTTATTCAGGCTCTACTTCGCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCa < 2:83977/139‑1 (MQ=255)
tgatgatgaAGGAAACCTCGTACCGATTAATCGTCCTCCAGCAAACTGGAAACGGCTTTATTCAGGCTCTACTTCGCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCAATGcc < 1:498982/139‑1 (MQ=255)
gatgatgaAGGAAACCTCGTACCGATTAATCGTCCTCCAGCAAACTGGAAACGGCTTTATTCAGGCTCTACTTCGCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCAATGccc < 1:231223/139‑1 (MQ=255)
atgaAGGAAACCTCGTACCGATTAATCGTCCTCCAGCAAACTGGAAACGGCTTTATTCAGGCTCTACTTCGCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCAATGCCCAGGc < 2:460984/139‑1 (MQ=255)
atgaAGGAAACCTCGTACCGATTAATCGTCCTCCAGCAAACTGGAAACGGCTTTATTCAGGCTCTACTTCGCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCAATGCCCAGGc < 1:317775/139‑1 (MQ=255)
aGGAAACCTCGTACCGATTAATCGTCCTCCAGCAAACTGGAAACGGCTTTATTCAGGCTCTACTTCGCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCAATGCCCAGGCCTgg < 2:92329/139‑1 (MQ=255)
ggAAACCTCGTACCGATTAATCGTCCTCCAGCAAACTGGAAACGGCTTTATTCAGGCTCTACTTCGCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCAATGCCCAGGCCTGGt < 1:166066/139‑1 (MQ=255)
gTACCGATTAATCGTCCTCCAGCAAACTGGAAACGGCTTTATTCAGGCTCTACTTCGCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCAATGCCCAGGCCTGGTTCTCTTTaa < 1:361210/139‑1 (MQ=255)
tACCGATTAATCGTCCTCCAGCAAACTGGAAACGGCTTTATTCAGGCTCTACTTCGCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCAATGCCCAGGCCTGGTTCTCTTTAAc < 1:372913/139‑1 (MQ=255)
tACCGATTAATCGTCCTCCAGCAAACTGGAAACGGCTTTATTCAGGCTCTACTTCGCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCAATGCCCAGGCCTGGTTCTCTTTAAc < 2:127257/139‑1 (MQ=255)
cGATTAATCGTCCTCCAGCAAACTGGAAACGGCTTTATTCAGGCTCTACTTCGCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCAATGCCCAGGCCTGGTTCTCTTTAACCTa < 1:179949/139‑1 (MQ=255)
ctccAGCAAACTGGAAACGGCTTTATTCAGGCTCTACTTCGCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCAATGCCCAGGcc > 1:46276/1‑110 (MQ=255)
ctccAGCAAACTGGAAACGGCTTTATTCAGGCTCTACTTCGCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCAATGCCCAGGcc < 2:46276/110‑1 (MQ=255)
cAGCAAACTGGAAACGGCTTTATTCAGGCTCTACTTCGCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCAATGCCCAGGCCTGGTTCTCTTTAACCTATCAGTAATTGTTcag > 1:245832/1‑139 (MQ=255)
gCAAACTGGAAACGGCTTTATTCAGGCTCTACTTCGCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCAATGCCCAGGCCTGGTTCTCTTTAACCTATCAGTAATTGTTcagca > 1:14165/1‑139 (MQ=255)
gCTTTATTCAGGCTCTACTTCGCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCAATGCCCAGGCCTGGTTCTCTTTAACCTATCAGTAATTGTTCAGCAGATAATGTGATaac < 2:245832/139‑1 (MQ=255)
tttACTCAGGCTCTACTTCGCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCAATGCCCAGGCCTGGTTCTCTTTAACCTATCAGTAATTGTTCAGCAGATAATGTGATaacag < 2:329457/139‑1 (MQ=255)
ggCTCTACTTCGCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCAATGCCCAGGCCTGGTTCTCTTTAACCTATCAGTAATTGTTCAGCAGATAATGTGATAacaggaacagga < 2:144310/139‑1 (MQ=255)
tACATTTCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCAATGCCCAGGCCTGGTTCTCTTTAACCTATCAGTAATTGTTCAGCAGATAATGTGATAACAGGAACAGGACAGTGAGtaataa > 2:280960/1‑139 (MQ=255)
cGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCAATGCCCAGGCCTGGTTCTCTTTAACCTATCAGTAATTGTTCAGCAGATAATGTGATAACAGGAACAGGACAGTGAGTAATAAAAACGTCAAt > 1:307466/1‑139 (MQ=255)
|
AGGGGCCAGGGATAGCCACCAATATTGGCGTAGCGTTGTTTGATGATGAAGGAAACCTCGTACCGATTAATCGTCCTCCAGCAAACTGGAAACGGCTTTATTCAGGCTCTACTTCGCTACATTTCATCGCCAAATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCAATGCCCAGGCCTGGTTCTCTTTAACCTATCAGTAATTGTTCAGCAGATAATGTGATAACAGGAACAGGACAGTGAGTAATAAAAACGTCAAT > NC_000913/4544059‑4544324
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
