Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F1 I1 R1	83	73.5	3575638	92.3%	3300313	107.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,474,310	C→T	G30G (GGC→GGT)	ydbC →	putative NAD(P)‑binding oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,474,310	0	C	T	100.0%	307.0 / NA	89	G30G (GGC→GGT)	ydbC	putative NAD(P)‑binding oxidoreductase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (42/47); total (42/47)

CTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCAGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGCCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTT  >  NC_000913/1474176‑1474443

cTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCAGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGtcccc                                                                                                                                   <  2:16387/139‑1 (MQ=255)

     cTGATTTTTCTTAATCCGTTTTATTACAGGGCAGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACga                                                                                                                              <  2:1671660/139‑1 (MQ=255)

        aTTTTTCTTAATCCGTTTTATTACAGGGCAGGGTGCGATGAGCAGCAATACATTTA‑‑CTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCg                                                                                                                         >  1:745133/1‑139 (MQ=255)

              cTTAATCCGTTTTATTACAGGGCAGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCAc                                                                                                                     <  2:967010/139‑1 (MQ=255)

              cTTAATCCGTTTTATTACAGGGCAGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCAc                                                                                                                     <  2:1131255/139‑1 (MQ=255)

                   tCCGTTTTATTACAGGGCAGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCACGTCGc                                                                                                                <  2:1693846/139‑1 (MQ=255)

                       ttttATTACAGGGCAGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCACGTCGCtata                                                                                                            >  1:1119111/1‑139 (MQ=255)

                                 gggcagggTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCACGTCGCTATAACCGtgctgc                                                                                                  >  2:1258435/1‑139 (MQ=255)

                                 gggcagggTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCACGTCGCTATAACCGtgctgc                                                                                                  >  1:1437123/1‑139 (MQ=255)

                                 gggcagggTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCACGTCGCTATAACCGtgctgc                                                                                                  >  2:1411514/1‑139 (MQ=255)

                                  ggcagggTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCg                                                                                                 >  1:1457294/1‑139 (MQ=255)

                                    cagggTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACAGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCACGTCGCTTTAACCGTGCTGCGTg                                                                                               <  1:1192876/139‑1 (MQ=255)

                                      gggTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAg                                                                                             >  2:490577/1‑139 (MQ=255)

                                         tGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGgcg                                                                                          <  2:360848/139‑1 (MQ=255)

                                         tGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGgcg                                                                                          <  1:775819/139‑1 (MQ=255)

                                         tGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGgcg                                                                                          >  2:775819/1‑139 (MQ=255)

                                         tGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGgcg                                                                                          <  1:1263412/139‑1 (MQ=255)

                                            gATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTg                                                                                       >  1:1471442/1‑139 (MQ=255)

                                             aTGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTgg                                                                                      >  2:1507974/1‑139 (MQ=255)

                                              tGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGc                                                                                     >  1:1334364/1‑139 (MQ=255)

                                                 gcagcaATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCAtt                                                                                  >  1:1396278/1‑139 (MQ=255)

                                                       aTACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGt                                                                            <  2:1457294/139‑1 (MQ=255)

                                                       aTACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGt                                                                            <  1:490577/139‑1 (MQ=255)

                                                          cATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCaa                                                                         >  1:998109/1‑139 (MQ=255)

                                                            tttACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATc                                                                       <  1:596810/139‑1 (MQ=255)

                                                            tttACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATc                                                                       <  1:510080/139‑1 (MQ=255)

                                                              tACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCat                                                                     <  2:1119111/139‑1 (MQ=255)

                                                               aCTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGt                                                                            <  1:1292530/131‑1 (MQ=255)

                                                               aCTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGt                                                                            >  2:1292530/1‑131 (MQ=255)

                                                               aCTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAAt                                                                        >  2:1283504/1‑135 (MQ=255)

                                                               aCTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAAt                                                                        <  1:1283504/135‑1 (MQ=255)

                                                               aCTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCata                                                                    >  1:1609755/1‑139 (MQ=255)

                                                                    cGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGAt                                                               >  1:1327682/1‑139 (MQ=255)

                                                                    cGGTACAAAACCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGAt                                                               >  1:1296961/1‑139 (MQ=255)

                                                                     ggTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGa                                                                                              <  1:318608/107‑1 (MQ=255)

                                                                     ggTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGa                                                                                              >  2:318608/1‑107 (MQ=255)

                                                                     ggTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGgcg                                                                                          <  2:1682533/111‑1 (MQ=255)

                                                                     ggTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGgcg                                                                                          >  1:1682533/1‑111 (MQ=255)

                                                                        aCAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCa                                                           <  2:1471442/139‑1 (MQ=255)

                                                                          aaaaTCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGc                                                         <  1:1411514/139‑1 (MQ=255)

                                                                          aaaaTCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGc                                                         <  2:1334364/139‑1 (MQ=255)

                                                                               ccGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGc                                                         <  2:773620/134‑1 (MQ=255)

                                                                               ccGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGc                                                         >  1:773620/1‑134 (MQ=255)

                                                                               ccGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACtt                                                    >  2:903108/1‑139 (MQ=255)

                                                                                 gTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACtttt                                                  >  1:1099004/1‑139 (MQ=255)

                                                                                       cGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTc                                            <  2:1327682/139‑1 (MQ=255)

                                                                                         tCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCAc                                                                                                                     >  2:1731780/1‑64 (MQ=255)

                                                                                         tCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCAc                                                                                                                     <  1:1731780/64‑1 (MQ=255)

                                                                                         tCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCACg                                                                                                                    <  1:713669/65‑1 (MQ=255)

                                                                                         tCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCACg                                                                                                                    >  2:713669/1‑65 (MQ=255)

                                                                                          cTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGc                                         >  1:1246084/1‑139 (MQ=255)

                                                                                           ttGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCa                                        <  2:998109/139‑1 (MQ=255)

                                                                                           ttGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCa                                        <  1:1258435/139‑1 (MQ=255)

                                                                                           ttGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCa                                        <  1:1507974/139‑1 (MQ=255)

                                                                                              gTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGt                                     <  2:1609755/139‑1 (MQ=255)

                                                                                               ttATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTc                                    <  1:702420/139‑1 (MQ=255)

                                                                                                   ggCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTgg                                                                                >  2:234299/1‑91 (MQ=255)

                                                                                                   ggCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTgg                                                                                <  1:234299/91‑1 (MQ=255)

                                                                                                   ggCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATc                                                                       <  1:141377/100‑1 (MQ=255)

                                                                                                   ggCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATc                                                                       >  2:141377/1‑100 (MQ=255)

                                                                                                    gcgcGATGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTg                                                                                               <  2:339579/75‑1 (MQ=255)

                                                                                                    gcgcGATGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTg                                                                                               >  1:339579/1‑75 (MQ=255)

                                                                                                       cgATGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCACGt                                                                                                                   <  2:733486/52‑1 (MQ=255)

                                                                                                       cgATGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCACGt                                                                                                                   >  1:733486/1‑52 (MQ=255)

                                                                                                         aTGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCAc                                                                                                                     <  2:86738/48‑1 (MQ=255)

                                                                                                         aTGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCAc                                                                                                                     >  1:86738/1‑48 (MQ=255)

                                                                                                         aTGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGgcg                                                                                          <  2:1091933/75‑1 (MQ=255)

                                                                                                         aTGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGgcg                                                                                          >  1:1091933/1‑75 (MQ=255)

                                                                                                         aTGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTa                                                 >  2:1526395/1‑116 (MQ=255)

                                                                                                         aTGCAACTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTa                                                 <  1:1526395/116‑1 (MQ=255)

                                                                                                              aCTGGCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATc                     >  2:1441906/1‑139 (MQ=255)

                                                                                                                  gCAGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCcgcg                 >  1:255265/1‑139 (MQ=255)

                                                                                                                    aGGTCCTGGAGTTTTTGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGaa               <  1:861010/139‑1 (MQ=255)

                                                                                                                       tCCTGGAGTTTTTGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGc                                                         <  2:1730425/94‑1 (MQ=255)

                                                                                                                       tCCTGGAGTTTTTGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGc                                                         >  1:1730425/1‑94 (MQ=255)

                                                                                                                         cTGGAGTTTTTGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGt                                                                                                <  2:1229466/53‑1 (MQ=255)

                                                                                                                         cTGGAGTTTTTGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGt                                                                                                >  1:1229466/1‑53 (MQ=255)

                                                                                                                         cTGGAGTTTTTGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAgcgcc          <  2:1296961/139‑2 (MQ=255)

                                                                                                                          tGGAGTTTTTGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCtt         <  1:1718522/139‑1 (MQ=255)

                                                                                                                          tGGAGTTTTTGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCtt         <  1:903108/139‑1 (MQ=255)

                                                                                                                          tGGAGTTTTTGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCtt         <  2:255265/139‑1 (MQ=255)

                                                                                                                                ttttGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGt                                                                            <  1:327092/66‑1 (MQ=255)

                                                                                                                                ttttGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGt                                                                            >  2:327092/1‑66 (MQ=255)

                                                                                                                                 tttGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGt                                                                            <  2:843358/65‑1 (MQ=255)

                                                                                                                                 tttGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGt                                                                            >  1:843358/1‑65 (MQ=255)

                                                                                                                                 tttGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCa                                        <  2:26291/101‑1 (MQ=255)

                                                                                                                                 tttGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCa                                        >  1:26291/1‑101 (MQ=255)

                                                                                                                                 tttGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCtt  >  1:991665/1‑139 (MQ=255)

                                                                                                                                  ttGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGt                                     >  2:1332882/1‑103 (MQ=255)

                                                                                                                                  ttGGTCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGt                                     <  1:1332882/103‑1 (MQ=255)

CTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCAGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGCCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTT  >  NC_000913/1474176‑1474443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A