Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I2 R1
|
67 |
27.9 |
1239648 |
95.3% |
1181384 |
112.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,032,253 |
G→A |
R296H (CGT→CAT) |
uacT → |
uric acid permease |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,032,253 | 0 | G | A | 100.0%
| 101.6
/ NA
| 31 | R296H (CGT→CAT) | uacT | uric acid permease |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (15/16); total (15/16) |
CCGTTTCCATTCTGACCATGACTGCCGTGTTAATCATCGTGTTTATCGAGTCAATGGGGATGTTCCTGGCACTGGGTGAAATAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCGTGTCGATGGCGTAGGGACAATGATAGGCGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCGTGACGCGCGTTCATAGCCGCTGGGTGTGTATTTCTTCGGGAATTA > NC_000913/3032127‑3032384
|
ccGTTTCCATTCTGACCATGACTGCCGTGTTAATCATCGTGTTTATCGAGTCAATGGGGATGTTCCTGGCACTGGGTGAAATAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCATGTCGATGGCGt < 2:11837/139‑1 (MQ=255)
cGTTTCCATTCTGACCATGACTGCCGTGTTAATCATCGTGTTTATCGAGTCAATGGGGATGTTCCTGGCACTGGGTGAAATAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCATGTCGATGGCGTa < 1:149200/139‑1 (MQ=255)
cGTTTCCATTCTGACCATGACTGCCGTGTTAATCATCGTGTTTATCGAGTCAATGGGGATGTTCCTGGCACTGGGTGAAATAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCATGTCGATGGCGTa < 1:527255/139‑1 (MQ=255)
tGACCATGACTGCCGTGTTAATCATCGTGTTTATCGAGTCAATGGGGATGTTCCTGGCACTGGGTGAAATAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCATGTCGATGGCGTAGGGACAATGAt > 2:337639/1‑139 (MQ=255)
gcaagtcTGGCACTGGGTGAAAAAGGCGGTCGGAAACTCTCTTCGCACGATATTATTCGCGGGCTGCATGTCGATGGCGTAGGGACAATGATAGGCGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACg < 1:314525/133‑1 (MQ=255)
aTGTTCCTGGCGCTGGGTGAAATAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCATGTCGATGGCGTAGGGACAATGATAGGCGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACg < 1:208256/139‑1 (MQ=255)
aTGTTCCTGGCACTGGGTGAAATAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCATGTCGATGGCGTAGGGACAATGATAGGCGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACg < 1:113350/139‑1 (MQ=255)
tCCTGGCACTGGGTGAAATAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCATGTCGATGGCGTAGGGACAATGATAGGCGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTgg < 2:397045/139‑1 (MQ=255)
ccTGGCACTGGGTGAAATAGTCGGGCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCATGTCGATGGCGTAGGGACAATGATAGGCGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGc < 2:389866/139‑1 (MQ=255)
ggCACTGGGTGAAATAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCATGTCGATGGCGTAGGGACAATGATAGGCGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTg > 2:58741/1‑139 (MQ=255)
gggTGAAATAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCATGTCGATGGCGTAgg > 1:373601/1‑69 (MQ=255)
gggTGAAATAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCATGTCGATGGCGTAgg < 2:373601/69‑1 (MQ=255)
ggTGAAATAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCATTTCGATGGCTTAGGGACATGGATAGGCGGCACGTTTAAAGCCTTCCCCCACACGCCATTTTTTCAAACCTTGGGCCGGGttctgt > 2:314525/1‑135 (MQ=255)
ggTGAAATAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCATGTCGATGGCGTAGGGACAATGATAGGCGGCa < 1:612626/85‑1 (MQ=255)
ggTGAAATAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCATGTCGATGGCGTAGGGACAATGATAGGCGGCa > 2:612626/1‑85 (MQ=255)
ggTGAAATAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCATGTCGATGGCGTAGGGACAATGATAGGCGGCACGTTTAACa < 1:68437/94‑1 (MQ=255)
ggTGAAATAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCATGTCGATGGCGTAGGGACAATGATAGGCGGCACGTTTAACa > 2:68437/1‑94 (MQ=255)
tcggtcgTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCATGTCGATGGCGTAGGGACAATGATAggcggc < 1:587558/74‑1 (MQ=255)
tcggtcgTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCATGTCGATGGCGTAGGGACAATGATAggcggc > 2:587558/1‑74 (MQ=255)
tcggtcgTAAACTCTCTTCGCACGATATTATTCGCGGACTGCACGTCGATGGCGTAGGGACAATGATAGGCGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCGTGACGCGCGt > 2:412472/1‑139 (MQ=255)
tAAACTCTCTTCGCACGATATTATTCGCGGGCTGCATGTCGATGGCGTAGGGACAATGATAGGCGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAACACGTTGGCCTGGTTAGCGTGACGCGCGTTCATAGc > 2:617779/1‑139 (MQ=255)
tctctTCGCACGATATTATTCGCGGGCTGCATGTCGATGGCGTAGGGACAATGATAGGCGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCGTGACGCGCGTTCATAGCCGCTg < 1:337639/139‑1 (MQ=255)
tctTCGCACGATATTATTCGCGGGCTGCATGTCGATGGCGTAGGGACAATGATAGGCGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCaaaa > 2:536696/1‑98 (MQ=255)
tctTCGCACGATATTATTCGCGGGCTGCATGTCGATGGCGTAGGGACAATGATAGGCGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCaaaa < 1:536696/98‑1 (MQ=255)
tattCGCGGGCTGCATGTCGATGGCGTAGGGACAATGATAGGCGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCGTGACGCGCGTTCATAGCCGCTGGGTGTGTATTTCTTCg > 1:8161/1‑139 (MQ=255)
ttCGCGGGCTGCATGTCGATGGCGTAGGGACAATGATAGGCGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCGTGACGCGCGTTCATAGCCGCTGGGTGTGTATTTCTTCggg > 1:85119/1‑139 (MQ=255)
gcgGGCTGCATGTCGATGGCGTAGGGACAATGATAGGCGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCGTGACGCGCGTTCATAGCCGCTGGgt > 1:95720/1‑121 (MQ=255)
gcgGGCTGCATGTCGATGGCGTAGGGACAATGATAGGCGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCGTGACGCGCGTTCATAGCCGCTGGgt < 2:95720/121‑1 (MQ=255)
gggCTGCATGTCGATGGCGTAGGGACAATGATAGGCGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACg < 2:513270/79‑1 (MQ=255)
gggCTGCATGTCGATGGCGTAGGGACAATGATAGGCGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACg > 1:513270/1‑79 (MQ=255)
gggCTGCATGTCGATGGCGTAGGGACAATGATAGGCGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCGTGACGCGCGTTCATAGCCGCTGGGTGTGTATTTCTTCGGGAATta > 2:352331/1‑139 (MQ=255)
|
CCGTTTCCATTCTGACCATGACTGCCGTGTTAATCATCGTGTTTATCGAGTCAATGGGGATGTTCCTGGCACTGGGTGAAATAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCGTGTCGATGGCGTAGGGACAATGATAGGCGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCGTGACGCGCGTTCATAGCCGCTGGGTGTGTATTTCTTCGGGAATTA > NC_000913/3032127‑3032384
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
