Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F1 I1 R1 67 31.4 1402038 96.1% 1347358 109.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,714,326 (T)9→8 intergenic (+55/‑51) dtpA → / → gstA dipeptide and tripeptide permease A/glutathionine S‑transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,714,3180T.100.0% 86.2 / NA 21intergenic (+47/‑59)dtpA/gstAdipeptide and tripeptide permease A/glutathionine S‑transferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (10/11);  total (10/11)

ATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCGCTT  >  NC_000913/1714191‑1714416
                                                                                                                               |                                                                                                  
aTGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCtttttt                                                                                               <  1:111361/133‑1 (MQ=255)
aTGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCtttttt                                                                                               >  2:111361/1‑133 (MQ=255)
     gctgACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAg                                                                                      >  1:28703/1‑136 (MQ=255)
     gctgACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAg                                                                                      <  2:28703/136‑1 (MQ=255)
     gctgACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCaa                                                                                   >  1:319609/1‑139 (MQ=255)
              gcCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTAcc                                                                          <  2:486141/139‑1 (MQ=255)
                            gCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACg                                                                                 <  2:590783/118‑1 (MQ=255)
                            gCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACg                                                                                 >  1:590783/1‑118 (MQ=255)
                                               tGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGa                                         <  2:319609/139‑1 (MQ=255)
                                                       aaGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTg                                 <  2:127214/139‑1 (MQ=255)
                                                                         tAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCa                                                                               >  2:592879/1‑75 (MQ=255)
                                                                         tAGCGTAAAAGTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCa                                                                               <  1:592879/75‑1 (MQ=255)
                                                                                  aTTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTAc                                            <  2:314215/101‑1 (MQ=255)
                                                                                  aTTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTAc                                            >  1:314215/1‑101 (MQ=255)
                                                                                      aGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCGCtt  >  1:339906/1‑139 (MQ=255)
                                                                                                        gCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGa                                         >  2:28192/1‑82 (MQ=255)
                                                                                                        gCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGa                                         <  1:28192/82‑1 (MQ=255)
                                                                                                        gCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGa                                      <  1:513730/85‑1 (MQ=255)
                                                                                                        gCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGa                                      >  2:513730/1‑85 (MQ=255)
                                                                                                          cGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGctctct       >  1:364673/1‑114 (MQ=255)
                                                                                                          cGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGctctct       <  2:364673/114‑1 (MQ=255)
                                                                                                                    tCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTa                            <  2:322270/83‑1 (MQ=255)
                                                                                                                    tCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTa                            >  1:322270/1‑83 (MQ=255)
                                                                                                                               |                                                                                                  
ATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCGCTT  >  NC_000913/1714191‑1714416

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A