Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I1 R1
|
67 |
31.4 |
1402038 |
96.1% |
1347358 |
109.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,980,232 |
C→T |
R135H (CGC→CAC) |
ygeA ← |
Asp/Glu_racemase family protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,980,232 | 0 | C | T | 100.0%
| 44.4
/ NA
| 14 | R135H (CGC→CAC) | ygeA | Asp/Glu_racemase family protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (9/5); total (9/5) |
CGCGTGACGCTTCGGTAAATTGCCCCAGACACAGTTCTTCAAAAATAATCTGATTAATTTTCGCCCGTTCATCCGCTTCAGGAATAAGACAATTGATGGAAAATTGTTCCGTCAGCCGCCCGCGATAAAAATCCTGTTCCATGGTGTAACGCGTACCCAGCAGCGCCACACGAGTCATTCCGGCCCCGGTAATTGCACGTCCGGTGGCATCCGCAATGTGTAAGAAAGGCAGAGTGCAACGTGACTCAATGGCATCCG > NC_000913/2980110‑2980367
|
cgcgTGACGCTTCGGTAAATTGCCCCAGACACAGTTCTTCAAAAATAATCTGATTAATTTTCGCCCGTTCATCCGCTTCAGGAATAAGACAATTGATGGAAAATTGTTCCGTCAGCCGCCCGTGATaaaaa < 1:639339/131‑1 (MQ=255)
cgcgTGACGCTTCGGTAAATTGCCCCAGACACAGTTCTTCAAAAATAATCTGATTAATTTTCGCCCGTTCATCCGCTTCAGGAATAAGACAATTGATGGAAAATTGTTCCGTCAGCCGCCCGTGATaaaaa > 2:639339/1‑131 (MQ=255)
taatCTGATTAATTTTCGCCCGTTCATCCGCTTCAGGAATAAGACAATTGATGGAAAATTGTTCCGTCAGCCGCCCGTGATAAAAATCCTGTTCCATGGTGTAACGCGTACCCAGCAGCGCCACACGAGTCATTCCGGc < 2:71894/139‑1 (MQ=255)
gTTCATCCGCTTCAGGAATAAGACAATTGATGGAAAATTGTTCCGTCAGCCGCCCGTGATAAAAATCCTGTTCCATGGTGTAACGCGTACCCAGCAGCGCCACACGAGTCATTCCGGCCCCGGTAATTGCACGTCCGGt < 2:163324/139‑1 (MQ=255)
tCAGGAATAAGACAATTGATGGAAAATTGTTCCGTCAGCCGCCCGTGATAAAAATCCTGTTCCATGGTGTAACGCGTACCCAGCAGCGCCACACGAGTCATTCCGGCCCCGGTAATTGCACGTCCGGTGGCATCCGCaa > 1:318374/1‑139 (MQ=255)
cAGGAATAAGACAATTGATGGAAAATTGTTCCGTCAGCCGCCCGTGATAAAAATCCTGTTCCATGGTGTAACGCGTACCCAGCAGCGCCACACGAGTCATTCCGGCCCCGGTAATTGCACGTCCGGTGGCATCCGCAAt > 1:20156/1‑139 (MQ=255)
gAATAAGACAATTGATGGAAAATTGTTCCGTCAGCCGCCCGTGATAAAAATCCTGTTCCATGGTGTAACGCGTACCCAGCAGCGCCACACGAGTCATTCCGGCCCCGGTAATTGCACGTCCGGTGGCATCCGCAAtgtg > 1:305716/1‑139 (MQ=255)
ttGATGGAAAATTGTTCCGTCAGCCGCCCGTGATAAAAATCCTGTTCCATGGTGTAACGCGTACCCAGCAGCGCCACACGAGTCATTCCGGCCCCGGTAATTGCACGTCCGGTGGCATCCGCAATGTGTAAGAAAGGCa > 1:289445/1‑139 (MQ=255)
aTGGAAAATTGTTCCGTCAGCCGCCCGTGATAAAAATCCTGTTCCATGGTGTAAc > 1:366847/1‑55 (MQ=255)
aTGGAAAATTGTTCCGTCAGCCGCCCGTGATAAAAATCCTGTTCCATGGTGTAAc < 2:366847/55‑1 (MQ=255)
ggAAAATTGTTCCGTCAGCCGCCCGTGATAAAAATCCTGTTCCATGGTGTAACGCGTACCCAGCAGCGCCACACGAGTCATTCCGGCCCCGGTAATTGCACGTCCGGTGGCATCCGCAATGTGTAAGAAAGGCAGAGTg > 1:154/1‑139 (MQ=255)
ggAAAATTGTTCCGTCAGCCGCCCGTGATAAAAATCCTGTTCCATGGTGTAACGCGTACCCAGCAGCGCCACACGAGTCATTCCGGCCCCGGTAATTGCACGTCCGGTGGCATCCGCAATGTGTAAGAAAGGCAGAGTg < 2:318374/139‑1 (MQ=255)
aGCCGCCCGTGATAAAAATCCTGTTCCATGGTGTAACGCGTACCCAGCAGCGCCACACGAGTCATTCCGGCCCCGGTAATTGCACGTCCGGTGGCATCCGCAATGTGTAAGAAAGGCAGAGTGCAACTTGACTCAATgg > 2:45246/1‑139 (MQ=255)
aGCCGCCCGTGATAAAAATCCTGTTCCATGGTGTAACGCGTACCCAGCAGCGCCACACGAGTCATTCCGGCCCCGGTAATTGCACGTCCGGTGGCATCCGCAATGTGTAAGAAAGGCAGAGTGCAACGTGACTCAATgg > 1:538891/1‑139 (MQ=255)
ccgtGATAAAAATCCTGTTCCATGGTGTAACGCGTACCCAGCAGAGACAAGGGAGTCCGTCGGGCCCCGGTAATTTCTAGAACGGAGGCATACGCATTGTGTAAACAAGGCAGAGTGCAATATGGCTCAATTGCATCCg < 1:13194/139‑1 (MQ=255)
|
CGCGTGACGCTTCGGTAAATTGCCCCAGACACAGTTCTTCAAAAATAATCTGATTAATTTTCGCCCGTTCATCCGCTTCAGGAATAAGACAATTGATGGAAAATTGTTCCGTCAGCCGCCCGCGATAAAAATCCTGTTCCATGGTGTAACGCGTACCCAGCAGCGCCACACGAGTCATTCCGGCCCCGGTAATTGCACGTCCGGTGGCATCCGCAATGTGTAAGAAAGGCAGAGTGCAACGTGACTCAATGGCATCCG > NC_000913/2980110‑2980367
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
