Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A0 F0 I1 R1
|
31 |
30.9 |
1275452 |
97.8% |
1247392 |
117.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,640,548 |
T→C |
intergenic (+3/‑314) |
uspA → / → dtpB |
universal stress global response regulator/dipeptide and tripeptide permease B |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,640,548 | 0 | T | C | 100.0%
| 94.8
/ NA
| 27 | intergenic (+3/‑314) | uspA/dtpB | universal stress global response regulator/dipeptide and tripeptide permease B |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (11/16); total (11/16) |
TTCTGGAGCAAACTGATGTCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCTTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGCCTTGTTACCTGATCAGCGTAAACACCTTATCTGGCCTACGGTCTGCGTACGCAATCAAAATCCCCAGCCAATACA > NC_000913/3640453‑3640682
|
ttCTGGAGCAAACTGATGTCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCCTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGtt < 1:235333/139‑1 (MQ=255)
gAGCAAACTGATGTCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCTGAGTGTTCCGCTGCGCGACGAAGAAGAATAATCCTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCt < 2:384255/139‑1 (MQ=255)
gTCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCCTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGcc < 1:182887/139‑1 (MQ=255)
gTCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCCTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGcc < 1:450243/139‑1 (MQ=255)
ttCCGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCCTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGCCtt < 2:248750/138‑1 (MQ=255)
ttCCGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCCTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGCCtt > 1:248750/1‑138 (MQ=255)
tCCGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCCTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGCCTTGt > 2:582814/1‑139 (MQ=255)
cAGCTCATCAACACGGTTCACGTTGGTATGCTGATTGTACCGCTGCGCGACGAAGAAAAATAAAGCCCCCTCTACGACGGGTGCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGCGTTGTTACCTGGTc < 2:617765/139‑1 (MQ=255)
ttCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCCTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGgcg > 1:208693/1‑106 (MQ=255)
ttCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCCTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGgcg < 2:208693/106‑1 (MQ=255)
ttCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCCTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGCCTTGTTACCTGATCAGCGTAAACACCTTAt < 2:491512/139‑1 (MQ=255)
ttCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCCTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGCCTTGTTACCTGATCAGCGTAAACACATTAt > 1:223066/1‑139 (MQ=255)
cACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCCTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGCCTTGTTACCTGATCAGCGTa > 1:344528/1‑127 (MQ=255)
cACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCCTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGCCTTGTTACCTGATCAGCGTa > 1:360097/1‑127 (MQ=255)
cACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCCTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGCCTTGTTACCTGATCAGCGTa < 2:360097/127‑1 (MQ=255)
cACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCCTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGCCTTGTTACCTGATCAGCGTa < 2:344528/127‑1 (MQ=255)
tGATTGTTCCGCTGCGCGACGAAGAAGAATAATCCTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCg > 2:550129/1‑76 (MQ=255)
tGATTGTTCCGCTGCGCGACGAAGAAGAATAATCCTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCg < 1:550129/76‑1 (MQ=255)
aTTGTTCCGCTGCGCGACGAAGAAGAATAATCCTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGcc < 2:101442/93‑1 (MQ=255)
aTTGTTCCGCTGCGCGACGAAGAAGAATAATCCTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGcc > 1:101442/1‑93 (MQ=255)
aTTGTTCCGCTGCGCGACGAAGAAGAATAATCCTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGcc < 1:483696/93‑1 (MQ=255)
aTTGTTCCGCTGCGCGACGAAGAAGAATAATCCTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGcc > 2:483696/1‑93 (MQ=255)
cGCTGCGCGACGAAGAAGAATAATCCTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGCCtt < 1:98406/88‑1 (MQ=255)
cGCTGCGCGACGAAGAAGAATAATCCTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGCCtt > 2:98406/1‑88 (MQ=255)
gacgaAGAAGAATAATCCTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGCCTTGTTACCTGATCAGCGTAAACACCTTATCTGGCCTACGGTCTGCGTACGCAATCAAAATc > 2:300065/1‑139 (MQ=255)
acgaAGAAGAATAATCCTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGCCTTGTTACCTGATCAGCGTAAACACCTTATCTGGCCTACGGTCTGCGTACGCAATCAAAATcc < 1:512404/139‑1 (MQ=255)
taatCCTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGCCTTGTTACCTGATCAGCGTAAACACCTTATCTGGCCTACGGTCTGCGTACGCAATCAAAATCCCCAGCCAATac < 1:140225/139‑1 (MQ=255)
aatCCTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGCCTTGTTACCTGATCAGCGTAAACACCTTATCTGGCCTACGGTCTGCGTACGCAATCAAAATCCCCAGCCAATaca < 1:86535/139‑1 (MQ=255)
|
TTCTGGAGCAAACTGATGTCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCTTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGCCTTGTTACCTGATCAGCGTAAACACCTTATCTGGCCTACGGTCTGCGTACGCAATCAAAATCCCCAGCCAATACA > NC_000913/3640453‑3640682
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
