Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F1 I1 R1 63 64.8 2934156 93.7% 2749304 111.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,161,267 G→A P468L (CCG→CTG)  ftsP ← septal ring component that protects the divisome from stress, multicopy suppressor of ftsI(Ts)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,161,2670GA100.0% 224.9 / NA 65P468L (CCG→CTG) ftsPseptal ring component that protects the divisome from stress, multicopy suppressor of ftsI(Ts)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (35/30);  total (35/30)

TAGAGCTCATTTTGCTTCCAGAAATGATAAAAAAATAATCAAAGGCCGGGGATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACAAGCAGCTCCACTTGTCCGTCAACCCAAAC  >  NC_000913/3161137‑3161400
                                                                                                                                  |                                                                                                                                     
tAGAGCTCATTTTGCTTCCAGAAATGATAAAAAAATAATCAAAGGCCGGGGATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCAGATTGAcc                                                                                                                               >  2:663688/1‑139 (MQ=255)
tAGAGCTCATTTTGCTTCCAGAAATGATAAAAAAATAATCAAAGGCCGGGGATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCAGATTGAcc                                                                                                                               <  1:663688/139‑1 (MQ=255)
   agCTCATTTTGCTTCCAGAAATGATAAAAAAATAATCAAAGGCCGGGGATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCAGATTGACCAAc                                                                                                                            >  1:553152/1‑139 (MQ=255)
     cTCATTTTGCTTCCAGAAATGATAAAAAAATAATCAAAGGCCGGGGATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCAGATTGACCAACAg                                                                                                                          >  2:790963/1‑139 (MQ=255)
        aTTTTGCTTCCAGAAATGATAAAAAAATAATCAAAGGCCGGGGATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCAGATTGACCAACAGTTg                                                                                                                       <  2:1217088/139‑1 (MQ=255)
         ttttGCTTCCAGAAATGATAAAAAAATAATCAAAGGCCGGGGATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCAGATTGAc                                                                                                                                <  2:608032/129‑1 (MQ=255)
         ttttGCTTCCAGAAATGATAAAAAAATAATCAAAGGCCGGGGATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCAGATTGAc                                                                                                                                >  1:608032/1‑129 (MQ=255)
          tttGCTTCCAGAAATGATAAAAAAATAATCAAAGGCCGGGGATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCAGATTGAcc                                                                                                                               <  1:1008023/129‑1 (MQ=255)
          tttGCTTCCAGAAATGATAAAAAAATAATCAAAGGCCGGGGATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCAGATTGAcc                                                                                                                               >  2:1008023/1‑129 (MQ=255)
                    gAAATGATAAAAAAATAATCAAAGGCCGGGGATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCAGATTGACCAACAGTTGCCCAATCGAGcc                                                                                                           <  2:553152/139‑1 (MQ=255)
                         gATAAAAAAATAATCAAAGGCCGGGGATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCAGATTGACCAACAGTTGCCCAATCGAGCCACGGt                                                                                                      <  1:850549/139‑1 (MQ=255)
                          aTAAAAAAATAATCAAAGGCCGGGGATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCAGAt                                                                                                                                    <  2:724507/108‑1 (MQ=255)
                          aTAAAAAAATAATCAAAGGCCGGGGATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCAGAt                                                                                                                                    >  1:724507/1‑108 (MQ=255)
                           tAAAAAAATAATCAAAGGCCGGGGATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCAGATTGACCAACAGTTGCCCAATCGAGCCACGGTcc                                                                                                    >  1:1395010/1‑139 (MQ=255)
                                          aGGCCGGGGATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCAGATTGACCAACAGTTGCTCaa                                                                                                                  <  1:998286/110‑1 (MQ=255)
                                          aGGCCGGGGATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCAGATTGACCAACAGTTGCCCaa                                                                                                                  >  2:998286/1‑110 (MQ=255)
                                                gggATTATAAGTCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCAGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTg                                                                               >  1:1363597/1‑139 (MQ=255)
                                                gggATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCAGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTg                                                                               >  1:104373/1‑139 (MQ=255)
                                                gggATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCAGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTg                                                                               <  1:790963/139‑1 (MQ=255)
                                                      ataAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCAGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAg                                                                         >  2:428337/1‑139 (MQ=255)
                                                       taAGTCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCAGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGt                                                                        <  2:1363597/139‑1 (MQ=255)
                                                       taAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCAGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGt                                                                        <  1:635572/139‑1 (MQ=255)
                                                       taAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCAGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGt                                                                        >  1:107005/1‑139 (MQ=255)
                                                         aGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCAGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAg                                                                      <  1:428337/139‑1 (MQ=255)
                                                          gCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCAGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGa                                                                     >  1:845341/1‑139 (MQ=255)
                                                           ccGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCAGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGaa                                                                    >  2:703118/1‑139 (MQ=255)
                                                            cGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCAGATTGACCAACAGTTGCCCAATCGAGCCa                                                                                                          >  1:359398/1‑100 (MQ=255)
                                                            cGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCAGATTGACCAACAGTTGCCCAATCGAGCCa                                                                                                          <  2:359398/100‑1 (MQ=255)
                                                             ggAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCAGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCg                                                                                                   >  1:1212962/1‑106 (MQ=255)
                                                             ggAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCAGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCg                                                                                                   <  2:1212962/106‑1 (MQ=255)
                                                             ggAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCAGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACg                                                                  >  1:615521/1‑139 (MQ=255)
                                                             ggAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCAGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACg                                                                  >  1:703087/1‑139 (MQ=255)
                                                               aaCGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCAGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACggg                                                                >  1:816780/1‑139 (MQ=255)
                                                                   aaaGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCAGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGt                                                            <  2:107005/139‑1 (MQ=255)
                                                                      gagaAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCAGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGCAGTAGAACGGGAAGTgcg                                                         <  2:816780/139‑1 (MQ=255)
                                                                                        ccATACTCGCCCTCCTCGGGCGAGTATCAAGATTACGGTACCAGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGa                                       <  2:845341/139‑1 (MQ=255)
                                                                                           tACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCAGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAAt                                    >  1:868970/1‑139 (MQ=255)
                                                                                             cTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCAGATTGAcc                                                                                                                               >  2:897820/1‑46 (MQ=255)
                                                                                             cTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCAGATTGAcc                                                                                                                               <  1:897820/46‑1 (MQ=255)
                                                                                             cTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCAGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATaa                                  >  1:900824/1‑139 (MQ=255)
                                                                                             cTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCAGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATaa                                  >  2:1164693/1‑139 (MQ=255)
                                                                                              tCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCAGATTGACCAACAGTTGCCCAAt                                                                                                                 <  2:298078/59‑1 (MQ=255)
                                                                                              tCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCAGATTGACCAACAGTTGCCCAAt                                                                                                                 >  1:298078/1‑59 (MQ=255)
                                                                                              tCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCAGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGt                                                                                      <  2:141693/86‑1 (MQ=255)
                                                                                              tCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCAGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGt                                                                                      >  1:141693/1‑86 (MQ=255)
                                                                                              tCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCAGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTg                                                                                   >  2:1346706/1‑89 (MQ=255)
                                                                                              tCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCAGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTg                                                                                   <  1:1346706/89‑1 (MQ=255)
                                                                                                gCCCTCCTCGGGCGAGTATGAAGATTACGGTACCAGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGAc                                                                                 >  1:1326813/1‑89 (MQ=255)
                                                                                                gCCCTCCTCGGGCGAGTATGAAGATTACGGTACCAGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGAc                                                                                 <  2:1326813/89‑1 (MQ=255)
                                                                                                gCCCTCCTCGGGCGAGTATGAAGATTACGGTACCAGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACa                               >  1:794960/1‑139 (MQ=255)
                                                                                                gCCCTCCTCGGGCGAGTATGAAGATTACGGTACCAGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACa                               >  2:184959/1‑139 (MQ=255)
                                                                                                 cccTCCTCGGGCGAGTATGAAGATTACGGTACCAGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGt                                                            >  1:681425/1‑109 (MQ=255)
                                                                                                 cccTCCTCGGGCGAGTATGAAGATTACGGTACCAGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGt                                                            <  2:681425/109‑1 (MQ=255)
                                                                                                         gggCGAGTATGAAGATTACGGTACCAGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTgcg                                                         >  2:1244474/1‑104 (MQ=255)
                                                                                                         gggCGAGTATGAAGATTACGGTACCAGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTgcg                                                         <  1:1244474/104‑1 (MQ=255)
                                                                                                                   gAAGATTACGGTACCAGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACAAGCAGCTCCACTTGTCCGt            <  2:1090716/139‑1 (MQ=255)
                                                                                                                        ttACGGTACCAGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACAAGCAGCTCCACTTGTCCGTCAAcc       <  2:794960/139‑1 (MQ=255)
                                                                                                                         tACGGTACCAGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACAAGCAGCTCCACTTGTCCGTCAAccc      >  2:454983/1‑139 (MQ=255)
                                                                                                                          aCGGTACCAGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGa                                           >  1:180674/1‑101 (MQ=255)
                                                                                                                          aCGGTACCAGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGa                                           <  2:180674/101‑1 (MQ=255)
                                                                                                                          aCGGTACCAGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACAAGCAGCTCCACTTGTCCGTCAACCCa     >  2:1087215/1‑139 (MQ=255)
                                                                                                                           cGGTACCAGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGATGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACAagcagc                         >  2:22413/1‑118 (MQ=255)
                                                                                                                           cGGTACCAGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACAagcagc                         <  1:22413/118‑1 (MQ=255)
                                                                                                                             gTACCAGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACAAGCAGCTCCACTTGTCCGTCAACCCaaat  <  1:184959/139‑2 (MQ=255)
                                                                                                                             gTACCAGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACAAGCAGCTCCACTTGTCCGTCAACCCAAAc  <  2:552495/139‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                     
TAGAGCTCATTTTGCTTCCAGAAATGATAAAAAAATAATCAAAGGCCGGGGATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACAAGCAGCTCCACTTGTCCGTCAACCCAAAC  >  NC_000913/3161137‑3161400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A