Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F1 I2 R1	70	59.9	2639336	94.5%	2494172	113.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,211,078	C→T	intergenic (+82/‑29)	rpsU → / → dnaG	30S ribosomal subunit protein S21/DNA primase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,211,078	0	C	T	100.0%	182.1 / NA	52	intergenic (+82/‑29)	rpsU/dnaG	30S ribosomal subunit protein S21/DNA primase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (27/25); total (27/25)

ACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATTCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTATTTTCGTTTATGAATTGCTAAAAATCGGGGCCTATGGCTGGACGAATCCCACGCGTATTCATTAATGATCTGCTGGCACGCACTGACATCGTCGATCTGATCGATGCCCGTGTGAAGCTGAAAAAGCAGGG  >  NC_000913/3210942‑3211204

aCGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATTCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTATTTTTGt                                                                                                                              >  2:243208/1‑139 (MQ=255)

       gcgAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATTCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTATTTTTGTTTATGaa                                                                                                                       <  1:608275/139‑1 (MQ=255)

        cgAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATTCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTATTTTTGTTTATGAAt                                                                                                                      >  1:1008746/1‑139 (MQ=255)

                     tCGCGAAAACGCACGCCGCACTCGTCTGTACTAATTCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTATTTTTGTTTATGAATTGCTAAAAATCgg                                                                                                         >  2:1286490/1‑139 (MQ=255)

                       gcgAAAACGCACGCCGCACTCGTCTGTACTAATTCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTATTTTTGTTTATGAATTGCTAAAAATCgggg                                                                                                       >  1:587059/1‑139 (MQ=255)

                        cgAAAACGCACGCCGCACTCGTCTGTACTAATTCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTATTTTTGTTTATGAATTGCTAAAAATCg                                                                                                          >  1:388539/1‑135 (MQ=255)

                        cgAAAACGCACGCCGCACTCGTCTGTACTAATTCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTATTTTTGTTTATGAATTGCTAAAAATCg                                                                                                          <  2:388539/135‑1 (MQ=255)

                        cgAAAACGCACGCCGCACTCGTCTGTACTAATTCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTATTTTTGTTTATGAATTGCTAAAAATCGGGGc                                                                                                      <  2:587059/139‑1 (MQ=255)

                             acgcacgcCGCACTCGTCTGTACTAATTCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTATTTTTGTTTATGAATTGCTAAAAATCGGGGCCTATg                                                                                                 >  1:321070/1‑139 (MQ=255)

                                cacgcCGCACTCGTCTGTACTAATTCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTATTTTTGTTTATGAATTGCTAAAAATCGGGGCCTATGGCt                                                                                              <  2:294401/139‑1 (MQ=255)

                                     cgcACTCGTCTGTACTAATTCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTATTTTTGTTTATGAATTGCTAAAAATCGGGGCCTATGGCTGGACg                                                                                         <  2:321070/139‑1 (MQ=255)

                                          tCGTCTGTACTAATTCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTATTTTTGTTTATGAATTGCTAAAAATCGGGGCCTATGGCTGGACGAATcc                                                                                    <  1:243208/139‑1 (MQ=255)

                                                  aCTAATTCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTATTTTTGTTTATGAATTGCTAAAAATCGGGGCCTATGGCTGGACGAATCCCACGCGTa                                                                            <  2:1031973/139‑1 (MQ=255)

                                                         ccccGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTATTTTTGTTTATGAATTGCTAAAAATc                                                                                                           >  1:6166/1‑101 (MQ=255)

                                                         ccccGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTATTTTTGTTTATGAATTGCTAAAAATc                                                                                                           <  2:6166/101‑1 (MQ=255)

                                                                     tGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTATTTTTGTTTATGAATTGCTAAAAATCGGGGCCTATGGCTGGACGAATCCCACGCGTATTCATTAATGATCTGCTgg                                                         <  2:1008746/139‑1 (MQ=255)

                                                                     tGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTATTTTTGTTTATGAATTGCTAAAAATCGGGGCCTATGGCTGGACGAATCCCAC‑CGTATTCATTAATGATCTGCTGgc                                                        >  1:580511/1‑139 (MQ=255)

                                                                      gCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTATTTTTGTTTATGAATTGCTAAAAATCGGGGCCTATGGCTGGACGAATCCCAC‑CGTATTCATTAATGATCTGCTGgca                                                       <  2:580511/139‑1 (MQ=255)

                                                                               tCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTATTTTTGTTTATGAATTGCTAAAAATCGGGGCCTATGGCTGGCCGAATCCCACGCGTATTCATGAATGATCTGCTGGCACGCACTGa                                               <  1:925690/139‑1 (MQ=255)

                                                                               tCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTATTTTTGTTTATGAATTGCTAAAAATCGGGGCCTATGGCTGGACGAATCCCACGCGTATTCATTAATGATCTGCTGGCACGCACTGa                                               >  1:262935/1‑139 (MQ=255)

                                                                                         ttgtagttgtaAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTATTTTTGTTTATGAATTGCTAAAAATCGGGGCCTATGGCTGGACGAATCCCACGCGTATTCATTAATGATCTGCTGGCACGCACTGACATCGTCGAt                                     >  1:607585/1‑139 (MQ=255)

                                                                                                 gtaAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTATTTTTGTTTATGAATTGCTAAAAATCGGGGCCTATGGCTGGACGAATCCCACGCGTATTCATTAATGATCTGCTGgca                                                       <  2:934345/113‑1 (MQ=255)

                                                                                                 gtaAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTATTTTTGTTTATGAATTGCTAAAAATCGGGGCCTATGGCTGGACGAATCCCACGCGTATTCATTAATGATCTGCTGgca                                                       >  1:934345/1‑113 (MQ=255)

                                                                                                  taAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTATTTTTGTTTATGAATTGCTAAAAATCGGGGCCTATGGCTGGACGAATCCCACGCGTATTCATTAATGATCTGCTGGCACGCACTGACATCGTCGATCTGATCGAt                            >  1:169282/1‑139 (MQ=255)

                                                                                                    aGGCCGTGCTTCCGAAAGGAATGCGCGGCTTATTTTTGTTTATGAATTGCTAAAAATCGGGGCCTATGGCTGGACGAATCCCACGCGTATTCATTAATGATctg                                                             >  1:556268/1‑104 (MQ=255)

                                                                                                    aGGCCGTGCTTCCGAAAGGAATGCGCGGCTTATTTTTGTTTATGAATTGCTAAAAATCGGGGCCTATGGCTGGACGAATCCCACGCGTATTCATTAATGATctg                                                             <  2:556268/104‑1 (MQ=255)

                                                                                                      gCCGTGCTTCCGAAAGGAATGCGCGGCTTATTTTTGTTTATGAATTGCTAAAAATCGGGGCCTATGGCTGGACGAATCCCACGCGTAtt                                                                          >  2:690739/1‑89 (MQ=255)

                                                                                                      gCCGTGCTTCCGAAAGGAATGCGCGGCTTATTTTTGTTTATGAATTGCTAAAAATCGGGGCCTATGGCTGGACGAATCCCACGCGTAtt                                                                          <  1:690739/89‑1 (MQ=255)

                                                                                                      gCCGTGCTTCCGAAAGGAATGCGCGGCTTATTTTTGTTTATGAATTGCTAAAAATCGGGGCCTATGGCTGGACGAATCCCACGCGTATTCATTAATGATCTGCTGgcacgcac                                                  >  2:948065/1‑113 (MQ=255)

                                                                                                      gCCGTGCTTCCGAAAGGAATGCGCGGCTTATTTTTGTTTATGAATTGCTAAAAATCGGGGCCTATGGCTGGACGAATCCCACGCGTATTCATTAATGATCTGCTGgcacgcac                                                  <  1:948065/113‑1 (MQ=255)

                                                                                                        cGTGCTTCCGAAAGGAATGCGCGGCTTATTTTTGTTTATGaa                                                                                                                       >  2:223065/1‑42 (MQ=255)

                                                                                                        cGTGCTTCCGAAAGGAATGCGCGGCTTATTTTTGTTTATGaa                                                                                                                       <  1:223065/42‑1 (MQ=255)

                                                                                                        cGTGCTTCCGAAAGGAATGCGCGGCTTATTTTTGTTTATGAATTGCTAAAAATCGGGGCCTATGGCTGGACGAATCCCa                                                                                  >  2:18063/1‑79 (MQ=255)

                                                                                                        cGTGCTTCCGAAAGGAATGCGCGGCTTATTTTTGTTTATGAATTGCTAAAAATCGGGGCCTATGGCTGGACGAATCCCa                                                                                  <  1:18063/79‑1 (MQ=255)

                                                                                                         gTGCTTCCGAAAGGAATGCGCGGCTTATTTTTGTTTATGaa                                                                                                                       >  1:410759/1‑41 (MQ=255)

                                                                                                         gTGCTTCCGAAAGGAATGCGCGGCTTATTTTTGTTTATGaa                                                                                                                       <  2:410759/41‑1 (MQ=255)

                                                                                                         gTGCTTCCGAAAGGAATGCGCGGCTTATTTTTGTTTATGAATTGCTAAAAATCGGGGCCTATGGc                                                                                               <  2:288247/65‑1 (MQ=255)

                                                                                                         gTGCTTCCGAAAGGAATGCGCGGCTTATTTTTGTTTATGAATTGCTAAAAATCGGGGCCTATGGc                                                                                               >  1:288247/1‑65 (MQ=255)

                                                                                                         gTGCTTCCGAAAGGAATGCGCGGCTTATTTTTGTTTATGAATTGCTAAAAATCGGGGCCTATGGCTGGACGAATCCCACGCGTATTCATTAATGATCTGCTGGCACGCACTGACATCGTCGATCTGATCGATGCCCgtg                     >  2:298884/1‑139 (MQ=255)

                                                                                                          tGCTTCCGAAAGGAATGCGCGGCTTATTTTTGTTTATGAATTGCTAAAAATCGGGGCCTATGGCTGGACGAATCCCACGCGTa                                                                            <  1:745553/83‑1 (MQ=255)

                                                                                                          tGCTTCCGAAAGGAATGCGCGGCTTATTTTTGTTTATGAATTGCTAAAAATCGGGGCCTATGGCTGGACGAATCCCACGCGTa                                                                            >  2:745553/1‑83 (MQ=255)

                                                                                                          tGCTTCCGAAAGGAATGCGCGGCTTATTTTTGTTTATGAATTGCTAAAAATCGGGGCCTATGGCTGGACGAATCCCACGCGTATTCATTAATGATCTGCTGGCACGCACTGACATCGTCGATCTGATCGATGCCCgtgt                    >  1:1093615/1‑139 (MQ=255)

                                                                                                             ttCCGAAAGGAATGCGCGGCTTATTTTTGTTTATGAATTGCTAAAAATCGGGGCCTATGGCTGGACGAATCCCACGCGTATTCATTAATGATCTGCTGGCACGCACTGACATCGTCGATCTGATCGATGCCCGTGTGaa                 <  2:169282/139‑1 (MQ=255)

                                                                                                             ttCCGAAAGGAATGCGCGGCTTATTTTTGTTTATGAATTGCTAAAAATCGGGGCCTATGGCTGGACGAATCCCACGCGTATTCATTAATGATCTGCTGGCACGCACTGACATCGTCGATCTGATCGATGCCCGTGTGaa                 <  1:1286490/139‑1 (MQ=255)

                                                                                                                cGAAAGGAATGCGCGGCTTATTTTTGTTTATGAATTGCTAAAAATCGGGGCCTATGGCTGGACGAATCCCACGCGTATTCATTAATGATCTGCTGGCACGCACTGACATCGTCGATCTGATCGATGCCCGTGTGAAGCt              >  2:1139171/1‑139 (MQ=255)

                                                                                                                    aGGAATGCGCGGCTTATTTTTGTTTATGAATTGCTAAAAATCg                                                                                                          >  1:1156048/1‑43 (MQ=255)

                                                                                                                    aGGAATGCGCGGCTTATTTTTGTTTATGAATTGCTAAAAATCg                                                                                                          <  2:1156048/43‑1 (MQ=255)

                                                                                                                         tGCGCGGCTTATTTTTGTTTATGAATTGCTAAAAATCGGGGCCTATGGCTGGACGAATCCCACGCGTATTCATTAATGATCTGCTGGCACGCACTGACATCGTCGATc                                    <  1:326988/108‑1 (MQ=255)

                                                                                                                         tGCGCGGCTTATTTTTGTTTATGAATTGCTAAAAATCGGGGCCTATGGCTGGACGAATCCCACGCGTATTCATTAATGATCTGCTGGCACGCACTGACATCGTCGATc                                    >  2:326988/1‑108 (MQ=255)

                                                                                                                         tGCGCGGCTTATTTTTGTTTATGAATTGCTAAAAATCGGGGCCTATGGCTGGACGAATCCCACGCGTATTCATTAATGATCTGCTGGCACGCACTGACATCGTCGATCTGATCGATGCCCGTGTGAAGCTGAAAAAGCa     >  1:389761/1‑139 (MQ=255)

                                                                                                                           cgcgGCTTATTTTTGTTTATGAATTGCTAAAAATCGGGGCCTATGGCTGGACGAATCCCACGCGTATTCATTAATGATCTGCTGGCACGCACTGACATCGTCGATCTGATCGATGCCCGTGTGAAGCTGAAAAAGCAgg   <  1:1139171/139‑1 (MQ=255)

                                                                                                                            gcgGCTTATTTTTGTTTATGAATTGCTAAAAATCGGGGCCTATGGCTGGACGAATCCCACGCGTATTCATTAATGATCTGCTGGCACGCACTGACATCGTCGATCTGATCGATGCCCGTGTGAAGCTGAAAAAGCAggg  >  1:378715/1‑139 (MQ=255)

ACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATTCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTATTTTCGTTTATGAATTGCTAAAAATCGGGGCCTATGGCTGGACGAATCCCACGCGTATTCATTAATGATCTGCTGGCACGCACTGACATCGTCGATCTGATCGATGCCCGTGTGAAGCTGAAAAAGCAGGG  >  NC_000913/3210942‑3211204

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A