Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A14 F1 I2 R1 70 59.9 2639336 94.5% 2494172 113.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,259,479 G→A G57G (GGC→GGT)  tdcG ← L‑serine dehydratase 3, anaerobic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,259,4790GA100.0% 193.3 / NA 55G57G (GGC→GGT) tdcGL‑serine dehydratase 3, anaerobic
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (33/22);  total (33/22)

ACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCTATAAATGCAGGGATCTCATCAATGACAACATCCTGCGGACTGTTTCCTGCCAGTCCCATGATGATGGCGACATCCGTGGCATGGCCTTTGCCCGTCAGTGACAACGACCCGTACAGATCGACCACAATATGGCTCGTCGCGGTTAATAAGCCGCTACTTTCCAGCCGATCAATAAAACTTTTTCCGGCATTCATTGGCCCCACGGTATGCG  >  NC_000913/3259347‑3259600
                                                                                                                                    |                                                                                                                         
aCCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCTATAAATGCAGGGATCTCATCAATGACAACATCCTGCGGACTGTTTCCTGCCAGTCCCATGATGATGGCGACATCCGTGGCATGGCCTTTACCCGTc                                                                                                                     >  2:638677/1‑139 (MQ=255)
         cACTGGCAGCCGCCCGCTGCGCGTTACTAACTCTATAAATGCAGGGATCTCATCAATGACAACATCCTGCGGACTGTTTCCTGCCAGTCCCATGATGATGGCGACATCCGTGGCATGGCCTTTACCCGTCAGTGACAAc                                                                                                            <  2:44726/139‑1 (MQ=255)
           cTGGCAGCCGCCCGCTGCGCGTTACTAACTCTATAAATGCAGGGATCTCATCAATGACAACATCCTGCGGACTGTTTCCTGCCAGTCCCATGATGATGGCGACATCCGTGGCATGGCCTTTACCCGTCAGTGACAACGa                                                                                                          <  1:99305/139‑1 (MQ=255)
                  ccgcccgcTGCGCGTTACTAACTCTATAAATGCAGGGATCTCATCAATGACAACATCCTGCGGACTGTTTCCTGCCAGTCCCATGATGATGGCGACATCCGTGGCATGGCCTTTACCCGTCAGTGACAACGACCCGTAc                                                                                                   >  2:1280400/1‑139 (MQ=255)
                          tGCGCGTTACTAACTCTATAAATGCAGGGATCTCATCAATGACAACATCCTGCGGACTGTTTCCTGCCAGTCCCATGATGATGGCGACATCCGTGGCATGGCCTTTACCCGTCAGTGACAACGACCCGTACAGATCGAc                                                                                           <  2:384725/139‑1 (MQ=255)
                                           ataAATGCAGGGATCTCATCAATGACAACATCCTGCGGACTGTTTCCTGCCAGTCCCATGATGATGGCGACATCCGTGGCATGGCCTTTACCCGTCAGTGACAACGACCCGTACAGATCGACCACAATATGGCtcgtcg                                                                          >  1:981511/1‑139 (MQ=255)
                                                  cAGGGATCTCATCAATGACAACATCCTGCGGACTGTTTCCTGCCAGTCCCATGATGATGGCGACATCCGTGGCATGGCCTTTACCCGTCAGTGACAACGAc                                                                                                         <  2:866531/101‑1 (MQ=255)
                                                  cAGGGATCTCATCAATGACAACATCCTGCGGACTGTTTCCTGCCAGTCCCATGATGATGGCGACATCCGTGGCATGGCCTTTACCCGTCAGTGACAACGAc                                                                                                         >  1:866531/1‑101 (MQ=255)
                                                  cAGGGATCTCATCAATGACAACATCCTGCGGACTGTTTCCTGCCAGTCCCATGATGATGGCGACATCCGTGGCATGGCCTTTACCCGTCAGTGACAACGACCCGTACAGATCGACCACAATATGGCTCGTCGCGGTtaa                                                                   >  1:250184/1‑139 (MQ=255)
                                                  cAGGGATCTCATCAATGACAACATCCTGCGGACTGTTTCCTGCCAGTCCCATGATGATGGCGACATCCGTGGCATGGCCTTTACCCGTCAGTGACAACGACCCGTACAGATCGACCACAATATGGCTCGTCGCGGTtaa                                                                   >  1:936860/1‑139 (MQ=255)
                                                  cAGGGATCTCATCAATGACAACATCCTGCGGACTGTTTCCTGCCAGTCCCATGATGATGGCGACATCCGTGGCATGGCCTTTACCCGTCAGTGACAACGACCCGTACAGATCGACCACAATATGGCTCGTCGCGGTtaa                                                                   >  2:431476/1‑139 (MQ=255)
                                                    gggATCTCATCAATGACAACATCCTGCGGACTGTTTCCTGCCAGTCCCATGATGATGGCGACATCCGTGGCATGGCCTTTACCCGTCAGTGACAACGACCCGTACAGa                                                                                                >  2:760994/1‑108 (MQ=255)
                                                    gggATCTCATCAATGACAACATCCTGCGGACTGTTTCCTGCCAGTCCCATGATGATGGCGACATCCGTGGCATGGCCTTTACCCGTCAGTGACAACGACCCGTACAGATCGACCACAATATGGCTCGTCGCGGTtaata                                                                 >  2:378137/1‑139 (MQ=255)
                                                    gggATCTCATCAATGACAACATCCTGCGGACTGTTTCCTGCCAGTCCCATGATGATGGCGACATCCGTGGCATGGCCTCTACCCGTCAGTGACAACGACCCGTACAGa                                                                                                <  1:760994/108‑1 (MQ=255)
                                                       aTCTCATCAATGACAACATCCTGCGGACTGTTTCCTGCCAGTCCCATGATGATGGCGACATCCGTGGCATGGCCTTTACCCGTCAGTGACAACGACCCGTACAGATCGACCACAATATGGCTCGTCGCGGTTAATAAGc                                                              <  1:431476/139‑1 (MQ=255)
                                                          tcatcaATGACAACATCCTGCGGACTGTTTCCTGCCAGTCCCATGATGATGGCGACATCCGTGGCATGGCCTTTACCCGTCAGTGACAACGACCCGTACAGATCGACCACAATATGGCTCGTCGCGGTTAATAAGCCGc                                                           >  2:532083/1‑139 (MQ=255)
                                                          tcatcaATGACAACATCCTGCGGACTGTTTCCTGCCAGTCCCATGATGATGGCGACATCCGTGGCATGGCCTTTACCCGTCAGTGACAACGACCCGTACAGATCGACCACAATATGGCTCGTCGCGGTTAATAAGCCGc                                                           <  2:250184/139‑1 (MQ=255)
                                                          tcatcaATGACAACATCCTGCGGACTGTTTCCTGCCAGTCCCATGATGATGGCGACATCCGTGGCATGGCCTTTACCCGTCAGTGACAACGACCCGTACAGATCGACCACAATATGGCTCGTCGCGGTTAATAAGCCGc                                                           <  1:80819/139‑1 (MQ=255)
                                                            atcaATGACAACATCCTGCGGACTGTTTCCTGCCAGTCCCATGATGATGGCGACATCCGTGGCATGGCCTTTACCCGTCAGTGACAACGACCCGTACAGATCGACCACAATATGGCTCGTCGCGGTTAATAAGCCGCTa                                                         >  1:90389/1‑139 (MQ=255)
                                                                   acaacaTCCTGCGGACTGTTTCCTGCCAGTCCCATGATGATGGCGACATCCGTGGCATGGCCTTTACCCGTCAGTGACAACGACCCGTACAGATCGACCACAATATGGCTCGTCACGGTTAATAAGCCGCTACTTTCCa                                                  >  1:648264/1‑139 (MQ=255)
                                                                    caacaTCCTGCGGACTGTTTCCTGCCAGTCCCATGATGATGGCGACATCCGTGGCATGGCCTTTACCCGTCAGTGACAACGACCCGTACAGATCGACCACAATATGGCTCGTCGCGGTTAATAAGCCGCTACTTTCCAg                                                 >  2:1030912/1‑139 (MQ=255)
                                                                      acaTCCTGCGGACTGTTTCCTGCCAGTCCCATGATGATGGCGACATCCGTGGCATGGCCTTTACCCGTCAGTGACAACGACCCGTACAGATCGACCACAATATGGCTCGTCGCGGTTAATAAGCCGCTACTTTCCAGcc                                               >  1:917962/1‑139 (MQ=255)
                                                                       caTCCTGCGGACTGTTTCCTGCCAGTCCCATGATGATGGCGACATCCGTGGCATGGCCTTTACCCGTCAGTGACAACGACCCGTACAGATCGACCACAATATGGCTCGTCGCGGTTAATAAGCCGCTACTTTCCAGCCg                                              <  1:532083/139‑1 (MQ=255)
                                                                       caTCCTGCGGACTGTTTCCTGCCAGTCCCATGATGATGGCGACATCCGTGGCATGGCCTTTACCCGTCAGTGACAACGACCCGTACAGATCGACCACAATATGGCTCGTCGCGGTTAATAAGCCGCTACTTTCCAGCCg                                              <  2:34454/139‑1 (MQ=255)
                                                                              cGGACTGTTTCCTGCCAGTCCCATGATGATGGCGACATCCGTGGCATGGCCTTTACCCGTCAGTGACAACGACCCGTACAGATCGACCACAATATGGCTCGTCGCGGTTAATAAGCCGCTACTTTCCAGCCGATCAATa                                       >  2:285453/1‑139 (MQ=255)
                                                                                     tttCCTGCCAGTCCCATGATGATGGCGACATCCGTGGCATGGCCTTTACCCGTCAGTGACa                                                                                                              >  2:280156/1‑61 (MQ=255)
                                                                                     tttCCTGCCAGTCCCATGATGATGGCGACATCCGTGGCATGGCCTTTACCCGTCAGTGACa                                                                                                              <  1:280156/61‑1 (MQ=255)
                                                                                     tttCCTGCCAGTCCCATGATGATGGCGACATCCGTGGCATGGCCTTTACCCGTCAGTGACAACGACCCGTACAGATCGAc                                                                                           >  2:1072718/1‑80 (MQ=255)
                                                                                     tttCCTGCCAGTCCCATGATGATGGCGACATCCGTGGCATGGCCTTTACCCGTCAGTGACAACGACCCGTACAGATCGAc                                                                                           <  1:1072718/80‑1 (MQ=255)
                                                                                           gCCAGTCCCATGATGATGGCGACATCCGTGGCATGGCCTTTACCCGTCAGTGACAACGACCCGTACAGATCGACCACAATATGGCTCGTCGCGGTTAATAAGCCGCTACTTTCCAGCCGATCAATAAAACTTTTTCCgg                          <  1:1280400/139‑1 (MQ=255)
                                                                                               gTCCCATGATGATGGCGACATCCGTGGCATGGCCTTTACCCGTCAGTGACAACGACCCGTACAGATCGACCACAATATGGCTCGTCGCGGTTAATAAGCCGCTACTTTCCAGCCGATCAATAAAACTTTTTCCGGcatt                      >  2:431114/1‑139 (MQ=255)
                                                                                                tCCCATGATGATGGCGACATCCGTGGCATGGCCTTTACCCGTCAGTGACAACGACCCGTACAGATCGACCACAATATGGCTCGTCGCGGTTAATAAGCCGCTACTTTCCAGCCGATCAATAAAACTTTTTCCGGcattc                     >  2:222144/1‑139 (MQ=255)
                                                                                                tCCCATGATGATGGCGACATCCGTGACATGGCCTTTACCCGTCAGTGACAACGACCCGTACAGATCGACCACAATATGGCTCGTCGCGGTTAATAAGCCGCTACTTTCCAGCCGATCAATAAAACTTTTTCCGGcattc                     >  2:108453/1‑139 (MQ=255)
                                                                                                  ccATGATGATGGCGACATCCGTGGCATGGCCTTTACCCGTCAGTGACAACGACCCGTACAGATCGACCACAATATGGCTCGTCGCGGTTAATAAGCCGCTACTTTCCAGCCGATCAATAAAACTTTTTCCGGcattcat                   <  2:89166/139‑1 (MQ=255)
                                                                                                     tgatgatgGCGACATCCGTGGCATGGCCTTTACCCGTCAGTGACAACGACCCGTACAGATCGACCACAATATGGCtcgtcg                                                                          <  2:478056/81‑1 (MQ=255)
                                                                                                     tgatgatgGCGACATCCGTGGCATGGCCTTTACCCGTCAGTGACAACGACCCGTACAGATCGACCACAATATGGCtcgtcg                                                                          >  1:478056/1‑81 (MQ=255)
                                                                                                      gatgatgGCGACATCCGTGGCATGGCCTTTACCCGTCAGTGACAACGACCCGTACAGATCGACCACAATATGGCTCGTCGCGGTTAATAAGCCg                                                            <  1:331976/94‑1 (MQ=255)
                                                                                                      gatgatgGCGACATCCGTGGCATGGCCTTTACCCGTCAGTGACAACGACCCGTACAGATCGACCACAATATGGCTCGTCGCGGTTAATAAGCCg                                                            >  2:331976/1‑94 (MQ=255)
                                                                                                          atgGCGACATCCGTGGCATGGCCTTTACCCGTCAGTGACAACGACCCGTACAGATCGACCACAATATGGCtcgtc                                                                           >  1:781084/1‑75 (MQ=255)
                                                                                                          atgGCGACATCCGTGGCATGGCCTTTACCCGTCAGTGACAACGACCCGTACAGATCGACCACAATATGGCtcgtc                                                                           <  2:781084/75‑1 (MQ=255)
                                                                                                          atgGCGACATCCGTGGCATGGCCTTTACCCGTCAGTGACAACGACCCGTACAGATCGACCACAATATGGCTCGTCGCGGTTAATAAGCCGCTACTTTCCAGCCGATCAATAAAACTTTTTCCGGCATTCATTGGCCCCa           >  1:470025/1‑139 (MQ=255)
                                                                                                            ggCGACATCCGTGGCATGGCCTTTACCCGTCAGTGACAACGACCCGTACAGATCGACCACAATATGGCTCGTCGCGGTTAATAAGCCGCTACTTTCCAGCCGATCAATAAAACTTTTTCCGGCATTCATTGGCCCCACg         >  1:206834/1‑139 (MQ=255)
                                                                                                            ggCGACATCCGTGGCATGGCCTTTACCCGTCAGTGACAACGACCCGTACAGATCGACCACAATATGGCTCGTCGCGGTTAATAAGCCGCTACTTTCCAGCCGATCAATAAAAC‑TTTTCCGGCATTCATTGGCCCCACgg        >  1:609953/1‑139 (MQ=255)
                                                                                                              cGACATCCGTGGCATGGCCTTTACCCGTCAGTGACAACGACCCGTACAGATCGACCACAATATGGCtcg                                                                             >  2:959863/1‑69 (MQ=255)
                                                                                                              cGACATCCGTGGCATGGCCTTTACCCGTCAGTGACAACGACCCGTACAGATCGACCACAATATGGCtcg                                                                             <  1:959863/69‑1 (MQ=255)
                                                                                                              cGACATCCGTGGCATGGCCTTTACCCGTCAGTGACAACGACCCGTACAGATCGACCACAATATGGCTCGTCGCGGTTAATAAGCCGCTACTTTCCAGCCGATCAATAAAACTTTTTCCGGCTTTCATTGGCCCCACGGt       >  1:1119095/1‑139 (MQ=255)
                                                                                                                aCATCCGTGGCATGGCCTTTACCCGTCAGTGACAACGACCCGTACAGATCGAc                                                                                           >  2:1137377/1‑53 (MQ=255)
                                                                                                                aCATCCGTGGCATGGCCTTTACCCGTCAGTGACAACGACCCGTACAGATCGAc                                                                                           <  1:1137377/53‑1 (MQ=255)
                                                                                                                aCATCCGTGGCATGGCCTTTACCCGTCAGTGACAACGACCCGTACAGATCGACCACAATATGGCTCGTCGCGGTTAATAAGCCGCTACTTTCCAGCCGATCAATAAAACTTTTTCCGGCATTCATTGGCCCCACGGTAt     >  1:1070401/1‑139 (MQ=255)
                                                                                                                 cATCCGTGGCATGGCCTTTACCCGTCAGTGACAACGACCCGTACAGATCGACCACAATATGGCTCGTCGCGGTTAATAAGCCGCTACTTTCCAGCCGATCAATAAAACTTTTTCCGGCATTCATTGGCCCCACGGTATg    >  2:1002940/1‑139 (MQ=255)
                                                                                                                   tCCGTGGCATGGCCTTTACCCGTCAGTGACAACGACCCGTACAGATCGACCACAATATGGCTCGTCGCGGTTAATAAGCCGCTACTTTCCAGCCGATCAATAAAACTTTTTCCGGCATTCATTGGCCCCACGGTATGCg  <  1:1030912/139‑1 (MQ=255)
                                                                                                                     cGTGGCATGGCCTTTACCCGTCAGTGACAACGACCCGTACAGATCGACCACAATATGGCTCGTCGCGGTTAATAAGCCg                                                            <  2:1189468/79‑1 (MQ=255)
                                                                                                                     cGTGGCATGGCCTTTACCCGTCAGTGACAACGACCCGTACAGATCGACCACAATATGGCTCGTCGCGGTTAATAAGCCg                                                            >  1:1189468/1‑79 (MQ=255)
                                                                                                                       tGGCATGGCCTTTACCCGTCAGTGACAACGACCCGTACAGATCGACCACAATATGGCtcgt                                                                            <  1:935400/61‑1 (MQ=255)
                                                                                                                       tGGCATGGCCTTTACCCGTCAGTGACAACGACCCGTACAGATCGACCACAATATGGCtcgt                                                                            >  2:935400/1‑61 (MQ=255)
                                                                                                                                    |                                                                                                                         
ACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCTATAAATGCAGGGATCTCATCAATGACAACATCCTGCGGACTGTTTCCTGCCAGTCCCATGATGATGGCGACATCCGTGGCATGGCCTTTGCCCGTCAGTGACAACGACCCGTACAGATCGACCACAATATGGCTCGTCGCGGTTAATAAGCCGCTACTTTCCAGCCGATCAATAAAACTTTTTCCGGCATTCATTGGCCCCACGGTATGCG  >  NC_000913/3259347‑3259600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A