Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F1 I2 R1	70	59.9	2639336	94.5%	2494172	113.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,411,139	T→C	L287P (CTC→CCC)	dusB →	tRNA‑dihydrouridine synthase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,411,139	0	T	C	100.0%	182.9 / NA	49	L287P (CTC→CCC)	dusB	tRNA‑dihydrouridine synthase B
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (28/21); total (28/21)

ACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCAGTTTCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAAC  >  NC_000913/3411004‑3411269

acacTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGTATCCCCa                                                                                                                                 >  1:797643/1‑139 (MQ=255)

    tGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGTATCCCCAGGaa                                                                                                                             >  1:1098954/1‑139 (MQ=255)

                      gCTGCCTTTGGCAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGTATCCCCAGGAACACGCTCCAAATGACCAg                                                                                                           >  1:1221579/1‑139 (MQ=255)

                       cTGCCTTTGGCAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGTATCCCCa                                                                                                                                 >  1:804370/1‑116 (MQ=255)

                       cTGCCTTTGGCAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGCATCCCCa                                                                                                                                 <  2:804370/116‑1 (MQ=255)

                                       ttAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGTATCCCCAGGAACACGCTCCAAATGACCAGTTTCGGCGCACATTCaa                                                                                          <  2:1098954/139‑1 (MQ=255)

                                         aaGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGTATCCCCAGGAACACGCTCCAAATGACCAGTTTCGGCGCACATTCAACg                                                                                        >  2:981811/1‑139 (MQ=255)

                                          aGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGTATCCCCAGGAACACGCTCCAAATGACCAGTTTCGGCGca                                                                                                 <  2:928499/129‑1 (MQ=255)

                                          aGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATAGTCCGGCAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGTATCCCCAGGAACACGCTCCAAATGACCAGTTTCGGCGca                                                                                                 >  1:928499/1‑129 (MQ=255)

                                              cttgcttTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGTATCCCCAGGAac                                                                                                                            >  1:1302581/1‑98 (MQ=255)

                                              cttgcttTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGTATCCCCAGGAac                                                                                                                            <  2:1302581/98‑1 (MQ=255)

                                                tgcttTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGTATCCCCAGGAACACGCTCCaaa                                                                                                                  >  1:77067/1‑106 (MQ=255)

                                                tgcttTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGTATCCCCAGGAACACGCTCCaaa                                                                                                                  <  2:77067/106‑1 (MQ=255)

                                                tgcttTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGTATCCCCAGGAACACGCTCCAAATGACCAGTTTCGGCGCACATTCAACGCCATTGa                                                                                 >  2:1136281/1‑139 (MQ=255)

                                                               ttCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGTATCCCCAGGAACACGCTCCAAATGACCAGTTTCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACa                                                                  >  1:676007/1‑139 (MQ=255)

                                                                   ggAACTGCATGACTTTTATGGTCCGGCAAAAGGGTTCCGAATTGCACGTAAACACGTTTCCTGGTATCCCCAGGAACACGCTCCAAATGACCAGTTTCGGCGCACATTCAACGCCATTGGGGATGCCAGCGAACAGCTg                                                              <  2:877589/139‑1 (MQ=255)

                                                                    gAACTGCATGACTTTTATGGTCCGGCAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGTATCCCCAGGAACACGCTCCAAATGACCAGTTTCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTgg                                                             <  2:797643/139‑1 (MQ=255)

                                                                      aCTGCATGACTTTTATGGTCCGGCAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGTATCCCCAGGAACACGCTCCAAATGACCAGTTTCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGAg                                                           <  1:996528/139‑1 (MQ=255)

                                                                          caggtcTTTTATGGTCCGGCAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGTATCCCCAGGAACACGCTCCAAATGACCAGTTTCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGAGGCGt                                                       >  1:764729/6‑139 (MQ=255)

                                                                          cATGACTTTTATGGTCCGGCAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGTATCCCCAGGAACACGCTCCAAATGACCAGTTTCGGCGca                                                                                                 >  1:686292/1‑97 (MQ=255)

                                                                          cATGACTTTTATGGTCCGGCAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGTATCCCCAGGAACACGCTCCAAATGACCAGTTTCGGCGca                                                                                                 <  2:686292/97‑1 (MQ=255)

                                                                          cATGACTTTTATGGTCCGGCAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGTATCCCCAGGAACACGCTCCAAATGACCAGTTTCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGAGGCGt                                                       >  1:526591/1‑139 (MQ=255)

                                                                          cATGACTTTTATGGTCCGGCAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGTATCCCCAGGAACACGCTCCAAATGACCAGTTTCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGAGGCGt                                                       >  2:158349/1‑139 (MQ=255)

                                                                          cATGACTTTTATGGTCCGGCAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGTATCCCCAGGAACACGCTCCAAATGACCAGTTTCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGAGGCGt                                                       >  2:956017/1‑139 (MQ=255)

                                                                          cATGACTTTTATGGTCCGGCAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGTATCCCCAGGAACACGCTCCAAATGACCAGTTTCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGAGGCGt                                                       >  1:1185838/1‑139 (MQ=255)

                                                                            tGACTTTTATGGTCCGGCAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGTATCCCCAGGAACACGCTCCAAATGACCAGTTTCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGAGGCGTTg                                                     >  1:905970/1‑139 (MQ=255)

                                                                                  ttATGGTCCGGCAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGTATCCCCAGGAACACGCTCCAAATGACCAGTTTCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGAGGCGTTGGAGGCa                                               <  2:840679/139‑1 (MQ=255)

                                                                                    aTGGTCCGGCAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGTATCCCCAGGAACACGCTCCAAATGACCAGTTTCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGAGGCGTTGGAGGCATa                                             >  1:494305/1‑139 (MQ=255)

                                                                                         ccGGCAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGTATCCCCAGGAACACGCTCCAAATGACCAGTTTCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGAGGCGTTGGAGGCATACTTCg                                        >  2:851574/1‑139 (MQ=255)

                                                                                                      aCCGAATTGCACGTAAACACGTTTCCTGGTATCCCCAGGAACACGCTCCAAATGACCAGTTTCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTa                           <  1:956017/139‑1 (MQ=255)

                                                                                                      aCCGAATTGCACGTAAACACGTTTCCTGGTATCCCCAGGAACACGCTCCAAATGACCAGTTTCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTa                           <  2:233070/139‑1 (MQ=255)

                                                                                                      aCCGAATTGCACGTAAACACGTTTCCTGGTATCCCCAGGAACACGCTCCAAATGACCAGTTTCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTa                           <  1:1136281/139‑1 (MQ=255)

                                                                                                           aTTGCACGTAAACACGTTTCCTGGTATCCCCAGGAACACGCTCCAAATGACCAGTTTCGGCGCACATTCAACGCCATAGAGGATGCCAGTGAACAGCTGGAGGCGTTGGATGCATACTTCGAAAATTTTGCGTAAACAg                      <  1:851574/139‑1 (MQ=255)

                                                                                                            ttGCACGTAAACACGTTTCCTGGTATCCCCAGGAACACGCTCCAAATGACCAGTTTCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGa                     >  1:903143/1‑139 (MQ=255)

                                                                                                             tGCACGTAAACACGTTTCCTGGTATCCCCAGGAACACGCTCCAAATGACCAGTTTCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGAGGCGTTGGAGGCATACTTCGATAATTTTGCGTAAACAGaa                    >  2:128008/1‑139 (MQ=255)

                                                                                                               cACGTAAACACGTTTCCTGGTATCCCCAGGAACACGCTCCAAATGACCAGTTTCGGCGCACATTCaa                                                                                          <  2:1023247/67‑1 (MQ=255)

                                                                                                               cACGTAAACACGTTTCCTGGTATCCCCAGGAACACGCTCCAAATGACCAGTTTCGGCGCACATTCaa                                                                                          >  1:1023247/1‑67 (MQ=255)

                                                                                                               cACGTAAACACGTTTCCTGGTATCCCCAGGAACACGCTCCAAATGACCAGTTTCGGCGCACATTCaa                                                                                          <  1:776625/67‑1 (MQ=255)

                                                                                                               cACGTAAACACGTTTCCTGGTATCCCCAGGAACACGCTCCAAATGACCAGTTTCGGCGCACATTCaa                                                                                          >  2:776625/1‑67 (MQ=255)

                                                                                                                 cGTAAGCACGTTTCCTGGTATCCCCAGGAACACGCTCCAAATGACCAGTTTCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATaa                >  2:994213/1‑139 (MQ=255)

                                                                                                                 cGTAAACACGTTTCCTGGTATCCCCAGGAGCACGCTCCAAATGACCAGTTTCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATaa                >  2:638359/1‑139 (MQ=255)

                                                                                                                      acacGTTTCCTGGTATCCCCAGGAACACGCTCCAAATGACCAGTTTCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGc           <  2:905970/139‑1 (MQ=255)

                                                                                                                              ccTGGTATCCCCAGGAACACGCTCCAAATGACCAGTTTCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGa        >  2:680340/1‑134 (MQ=255)

                                                                                                                              ccTGGTATCCCCAGGAACACGCTCCAAATGACCAGTTTCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGa        <  1:680340/134‑1 (MQ=255)

                                                                                                                              ccTGGTATCCCCAGGAACACGCTCCAAATGACCAGTTTCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGaa   >  2:161171/1‑139 (MQ=255)

                                                                                                                               cTGGTATCCCCAGGAACACGCTCCAAATGACCAGTTTCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAAc  <  2:75282/139‑1 (MQ=255)

                                                                                                                               cTGGTATCCCCAGGAACACGCTCCAAATGACCAGTTTCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAAc  <  2:399591/139‑1 (MQ=255)

                                                                                                                                  gTATCCCCAGGAACACGCTCCAAATGACCAGTTTCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGAGGCGt                                                       >  2:674894/1‑83 (MQ=255)

                                                                                                                                  gTATCCCCAGGAACACGCTCCAAATGACCAGTTTCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGAGGCGt                                                       <  1:674894/83‑1 (MQ=255)

ACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCAGTTTCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAAC  >  NC_000913/3411004‑3411269

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A