Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F1 I2 R1 94 59.5 3027142 91.9% 2781943 108.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,474,356 G→A A109T (GCC→ACC)  yfdQ → CPS‑53 (KpLE1) prophage, uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,474,3560GA100.0% 191.2 / NA 55A109T (GCC→ACC) yfdQCPS‑53 (KpLE1) prophage, uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/26);  total (29/26)

TGACTTTACCCGTTATTCTAAAGATCTTGCAGATGAAGGCACCCGCTGCTTTATCGATGCTGATAATATGCGTGCCGTCAGTGTGCTTAACCTGGGTACTATTGATGAACCAGGTCACGCAGATAACACCGCCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATCACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGA  >  NC_000913/2474226‑2474480
                                                                                                                                  |                                                                                                                            
tGACTTTACCCGTTATTCTAAAGATCTTGCAGATGAAGGCACCCGCTGCTTTATCGATGCTGATAATATGCGTGCCGTCAGTGTGCTTAACCTGTGTACTATTGATGAACCAGGTCACGCAGATAACACCACCACtctc                                                                                                                      <  2:1064088/139‑1 (MQ=255)
        cccGTTATTCTAAAGATCTTGCAGATGAAGGCACCCGCTGCTTTATCGATGCTGATAATATGCGTGCCGTCAGTGTGCTTAACCTGGGTACTATTGATGAACCAGGTCACGCAGATAACACCACCACTCTCAAACTGaa                                                                                                              <  1:211946/139‑1 (MQ=255)
             tATTCTAAAGATCTTGCAGATGAAGGCACCCGCTGCTTTATCGATGCTGATAATATGCGTGCCGTCAGTGTGCTTAACCTGGGTACTATTGATGAACCAGGTCACGCAGATAACACCACCACTCTCAAACTGAAAAAGa                                                                                                         <  2:213416/139‑1 (MQ=255)
                  tAAAGATCTTGCAGATGAAGGCACCCGCTGCTTTATCGATGCTGATAATATGCGTGCCGTCAGTGTGCTTAACCTGGGTACTATTGATGAACCAGGTCACGCAGATAACACCACCACTCTCAAACTGAAAAAGACAGCa                                                                                                    <  1:460898/139‑1 (MQ=255)
                         cTTGCAGATGAAGGCACCCGCTGCTTTATCGATGCTGATAATATGCGTGCCGTCAGTGTGCTTAACCTGGGTACTATTGATGAACCAGGTCACGCAGATAACACCACCACTCTCAAACTGAAAAAGACAGCACCGTtct                                                                                             >  2:839618/1‑139 (MQ=255)
                                 tGAAGGCACCCGCTGCTTTATCGATGCTGATAATATGCGTGCCGTCAGTGTGCTTAACCTGGGTACTATTGATGAACCAGGTCACGCAGATAACACCACCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCtg                                                                                     >  2:659687/1‑139 (MQ=255)
                                 tGAAGGCACCCGCTGCTTTATCGATGCTGATAATATGCGTGCCGTCAGTGTGCTTAACCTGGGTACTATTGATGAACCAGGTCACGCAGATAACACCACCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCtg                                                                                     <  1:659687/139‑1 (MQ=255)
                                       cACCCGCTGCTTTATCGATGCTGATAATATGCGTGCCGTCAGTGTGCTTAACCTGGGTACTATTGATGAACCAGGTCACGCAGATAACACCACCACTCTCaaa                                                                                                                   >  2:341728/1‑103 (MQ=255)
                                       cACCCGCTGCTTTATCGATGCTGATAATATGCGTGCCGTCAGTGTGCTTAACCTGGGTACTATTGATGAACCAGGTCACGCAGATAACACCACCACTCTCaaa                                                                                                                   <  1:341728/103‑1 (MQ=255)
                                          ccGCTGCTTTATCGATGCTGATAATATGCGTGCCGTCAGTGTGCTTAACCTGGGTACTATTGATGAACCAGGTCACGCAGATAACAccacca                                                                                                                           <  2:236462/92‑1 (MQ=255)
                                          ccGCTGCTTTATCGATGCTGATAATATGCGTGCCGTCAGTGTGCTTAACCTGGGTACTATTGATGAACCAGGTCACGCAGATAACAccacca                                                                                                                           >  1:236462/1‑92 (MQ=255)
                                                     tCGATGCTGATAATATGCGTGCCGTCAGTGTGCTTAACCTGGGTACTATTGATGAACCAGGTCACGCAGATAACACCACCACTCTCAAACTGAAAAAGACa                                                                                                       <  2:83300/101‑1 (MQ=255)
                                                     tCGATGCTGATAATATGCGTGCCGTCAGTGTGCTTAACCTGGGTACTATTGATGAACCAGGTCACGCAGATAACACCACCACTCTCAAACTGAAAAAGACa                                                                                                       >  1:83300/1‑101 (MQ=255)
                                                     tCGATGCTGATAATATGCGTGCCGTCAGTGTGCTTAACCTGGGTACTATTGATGAACCAGGTCACGCAGATAACACCACCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCg                                                                 >  1:1317525/1‑139 (MQ=255)
                                                        aTGCTGATAATATGCGTGCCGTCAGTGTGCTTAACCTGGGTACTATTGATGAACCAGGTCACGCAGATAACACCACCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTaa                                                              >  2:1473802/1‑139 (MQ=255)
                                                           cTGATAATATGCGTGCCGTCAGTGTGCTTAACCTGGGTACTATTGATGAACCAGGTCACGCAGATAACACCACCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTc                                                           <  2:1230718/139‑1 (MQ=255)
                                                                       gTGCCGTCAGTGTGCTTAACCTGGGTACTATTGATGAACCAGGTCACGCAGATAACACCACCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATCACt                                               <  1:1473802/139‑1 (MQ=255)
                                                                        tGCCGTCAGTGTGCTTAACCTGGGTACTATTGATGAACCAGGTCACGCAGATAACACCACCACTCTCAAACTGaa                                                                                                              >  2:347217/1‑75 (MQ=255)
                                                                        tGCCGTCAGTGTGCTTAACCTGGGTACTATTGATGAACCAGGTCACGCAGATAACACCACCACTCTCAAACTGaa                                                                                                              <  1:347217/75‑1 (MQ=255)
                                                                        tGCCGTCAGTGTGCTTAACCTGGGTACTATTGATGAACCAGGTCACGCAGATAACACCACCACTCTCAAACTGAAAAAGa                                                                                                         >  1:1466174/1‑80 (MQ=255)
                                                                        tGCCGTCAGTGTGCTTAACCTGGGTACTATTGATGAACCAGGTCACGCAGATAACACCACCACTCTCAAACTGAAAAAGa                                                                                                         <  2:1466174/80‑1 (MQ=255)
                                                                        tGCCGTCAGTGTGCTTAACCTGGGTACTATTGATGAACCAGGTCACGCAGATAACACCACCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACg                                                                        >  2:165131/1‑113 (MQ=255)
                                                                        tGCCGTCAGTGTGCTTAACCTGGGTACTATTGATGAACCAGGTCACGCAGATAACACCACCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACg                                                                        <  1:165131/113‑1 (MQ=255)
                                                                        tGCCGTCAGTGTGCTTAACCTGGGTACTATTGATGAACCAGGTCACGCAGATAACACCACCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATCACTg                                              >  2:901199/1‑139 (MQ=255)
                                                                         gCCGTCAGTGTGCTTAACCTGGGTACTATTGATGAACCAGGTCACGCAGATAACACCACCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATCACTgg                                             <  1:839618/139‑1 (MQ=255)
                                                                          ccGTCAGTGTGCTTAACCTGGGTACTATTGATGAACCAGGTCACGCAGATAACACCACCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATCACTGGc                                            >  1:1189123/1‑139 (MQ=255)
                                                                                gtgtGCTTAACCTGGGTACTATTGATGAACCAGGTCACGCAGATAACACCACCACTCTCAAACTGAAAAAGACAg                                                                                                      <  2:427206/75‑1 (MQ=255)
                                                                                gtgtGCTTAACCTGGGTACTATTGATGAACCAGGTCACGCAGATAACACCACCACTCTCAAACTGAAAAAGACAg                                                                                                      >  1:427206/1‑75 (MQ=255)
                                                                                   tgCTTAACCTGGGTACTATTGATGAACCAGGTCACGCAGATAACACCACCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATCACTGGCAGAATGGAt                                   >  1:311613/1‑139 (MQ=255)
                                                                                     cTTAACCTGGGTACTATTGATGAACCAGGTCACGCAGATAACACCACCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATCACTGGCAGAATGGATTg                                 >  2:89259/1‑139 (MQ=255)
                                                                                        aaCCTGGGTACTATTGATGAACCAGGTCACGCAGATAACACCACCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATCACTGGCAGAATGGATTGAAg                              >  2:1056953/1‑139 (MQ=255)
                                                                                        aaCCTGGGTACTATTGATGAACCAGGTCACGCAGATAACACCACCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATCACTGGCAGAATGGATTGAAg                              >  2:111051/1‑139 (MQ=255)
                                                                                         aCCTGGGTACTATTGATGAACCAGGTCACGCAGATAACACCACCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATCACTGGCAGAATg                                      <  1:569673/130‑1 (MQ=255)
                                                                                         aCCTGGGTACTATTGATGAACCAGGTCACGCAGATAACACCACCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATCACTGGCAGAATg                                      >  2:569673/1‑130 (MQ=255)
                                                                                           cTGGGTACTATTGATGAACCAGGTCACGCAGATAACACCACCACTCTCAAACTGAAAAAGACAGCa                                                                                                    >  2:163138/1‑66 (MQ=255)
                                                                                           cTGGGTACTATTGATGAACCAGGTCACGCAGATAACACCACCACTCTCAAACTGAAAAAGACAGCa                                                                                                    <  1:163138/66‑1 (MQ=255)
                                                                                           cTGGGTACTATTGATGAACCAGGTCACGCAGATAACACCACCACTCTCAAACTGAAAAAGACAGCACCGTTctct                                                                                           <  1:337258/75‑1 (MQ=255)
                                                                                           cTGGGTACTATTGATGAACCAGGTCACGCAGATAACACCACCACTCTCAAACTGAAAAAGACAGCACCGTTctct                                                                                           >  2:337258/1‑75 (MQ=255)
                                                                                             gggTACTATTGATGAACCAGGTCACGCAGATAACACCACCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATCACTGGCAGAATGGATTGAAGACTgg                         >  1:175988/1‑139 (MQ=255)
                                                                                             gggTACTATTGATGAACCAGGTCACGCAGATAACACCACCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATCACTGGCAGAATGGATTGAAGACTgg                         >  1:1397448/1‑139 (MQ=255)
                                                                                             gggTACTATTGATGAACCAGGTCACGCAGATAACACCACCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATCACTGGCAGAATGGATTGAAGACTgg                         <  1:901199/139‑1 (MQ=255)
                                                                                               gTACTATTGATGAACCAGGTCACGCAGATAACACCACCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATCACTGGCAGAATGGATTGAAGACTGGGc                       <  1:892269/139‑1 (MQ=255)
                                                                                               gTACTATTGATGAACCAGGTCACGCAGATAACACCACCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATCACTGGCAGAATGGATTGAAGACTGGGc                       >  2:726288/1‑139 (MQ=255)
                                                                                               gTACTATTGATGAACCAGGTCACGCAGATAACACCACCACTCTCAAAATGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCTAGAAATCACTGGCAGAATGGATTGAAGACTGGGc                       <  2:311613/139‑1 (MQ=255)
                                                                                                   tATTGATGAACCAGGTCACGCAGATAACACCACCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATCACTGGCAGAATGGATTGAAGACTGGGCCGAc                   >  2:385626/1‑139 (MQ=255)
                                                                                                   tATTGATGAACCAGGTCACGCAGATAACACCACCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATCACTGGCAGAATGGATTGAAGACTGGGCCGAc                   >  2:724614/1‑139 (MQ=255)
                                                                                                     ttGATGAACCAGGTCACGCAGATAACACCACCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATCACTGGCAGAATGGATTGAAGACTGGGCCGACTa                 >  1:368714/1‑139 (MQ=255)
                                                                                                             ccAGGTCACGCAGATAACACCACCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATCACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTgg         >  2:1405269/1‑139 (MQ=255)
                                                                                                               aGGTCACGCAGATAACACCACCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAAc                                                             <  1:1403708/85‑1 (MQ=255)
                                                                                                               aGGTCACGCAGATAACACCACCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAAc                                                             >  2:1403708/1‑85 (MQ=255)
                                                                                                                ggTCACGCAGATAACACCACCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATCACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCt      <  1:1405269/139‑1 (MQ=255)
                                                                                                                 gTCACGCAGATAACACCACCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATCACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCtt     >  1:448789/1‑139 (MQ=255)
                                                                                                                  tCACGCAGATAACACCACCACTCTCAAACTGAAAAAGACTGCACTGTTCCCTGCGCTGTTGACTGTTAACGGCGAGCGTAACGCCCCGGAATAACTGACAGAATGGATTGAAGACTGGGCCGACTACGTTGTGGGCttt    <  2:643784/139‑1 (MQ=255)
                                                                                                                  tCACGCAGATAACACCACCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATCACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCttt    <  2:448789/139‑1 (MQ=255)
                                                                                                                    aCGCAGATAACACCACCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATCACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGa  <  2:368714/139‑1 (MQ=255)
                                                                                                                            aaCACCACCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATCACTg                                              >  1:1396030/1‑87 (MQ=255)
                                                                                                                            aaCACCACCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATCACTg                                              <  2:1396030/87‑1 (MQ=255)
                                                                                                                                  |                                                                                                                            
TGACTTTACCCGTTATTCTAAAGATCTTGCAGATGAAGGCACCCGCTGCTTTATCGATGCTGATAATATGCGTGCCGTCAGTGTGCTTAACCTGGGTACTATTGATGAACCAGGTCACGCAGATAACACCGCCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATCACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGA  >  NC_000913/2474226‑2474480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A