Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F1 I2 R1	94	59.5	3027142	91.9%	2781943	108.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,551,631	T→C	V281V (GTA→GTG)	malP ←	maltodextrin phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,551,631	0	T	C	100.0%	162.6 / NA	46	V281V (GTA→GTG)	malP	maltodextrin phosphorylase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (21/25); total (21/25)

TGCTCATCGATCAGCACGCGCAGCAGTTCTGGAATCGCGATAGTTGGGTGGGTATCGTTCAGCTGAATAACTTCGTAATCCGCCAGTTCGTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCG  >  NC_000913/3551497‑3551766

tGCTCATCGATCAGCACGCGCAGCAGTTCTGGAATCGCGATAGTTGGGTGGGTATCGTTCAGCTGAATAACTTCGTAATCCGCCAGTTCGTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCCACCg                                                                                                                                     >  1:741065/1‑139 (MQ=255)

 gCTCATCGATCAGCACGCGCAGCAGTTCTGGAATCGCGATAGTTGGGTGGGTATCGTTCAGCTGAATAACTTCGTAATCCGCCAGTTCGTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCCACCGa                                                                                                                                    >  1:1162698/1‑139 (MQ=255)

  cTCATCTATCAGCACGCGCAGCAGTTCTGGAATCGCGATATTTCGGTGGGTATCGTTCAGCTGAATAACTTCGCAATCCGCCAGTTCGTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCCACCGaa                                                                                                                                   <  2:978762/139‑1 (MQ=255)

      tCGATCAGCACGCGCAGCAGTTCTGGAATCGCGATAGTTGGGTGGGTATCGTTCAGCTGAATAACTTCGTAATCCGCCAGTTCGTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCCACCGAACAgg                                                                                                                               <  2:741065/139‑1 (MQ=255)

         aTCAGCACGCGCAGCAGTTCTGGAATCGCGATAGTTGGGTGGGTATCGTTCAGCTGAATAACTTCGTAATCCGCCAGTTCGTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCCACCGAACAGGcac                                                                                                                            <  1:803313/139‑1 (MQ=255)

                 gcgcAGCAGTTCTGGAATCGCGATAGTTGGGTGGGTATCGTTCAGCTGAATAACTTCGTAATCCGCCAGTTCGTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCCACCGAACAGGCACACTGGAAg                                                                                                                    <  1:1081210/139‑1 (MQ=255)

                                    gcgATAGTTGGGTGGGTATCGTTCAGCTGAATAACTTCGTAATCCGCCAGTTCGTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCCACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCa                                                                                                 >  2:857013/1‑139 (MQ=255)

                                             gggtgggtATCGTTCAGCTGAATAACTTCGTAATCCGCCAGTTCGTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCCACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTAc                                                                                        <  2:414671/139‑1 (MQ=255)

                                             gggtgggtATCGTTCAGCTGAATAACTTCGTAATCCGCCAGTTCGTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCCACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTAc                                                                                        <  2:1162698/139‑1 (MQ=255)

                                                   gtATCGTTCAGCTGAATAACTTCGTAATCCGCCAGTTCGTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCCACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAg                                                                                  >  2:581104/1‑139 (MQ=255)

                                                     aTCGTTCAGCTGAATAACTTCGTAATCCGCCAGTTCGTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCCACCGAACAGGCACACt                                                                                                                         >  2:126254/1‑98 (MQ=255)

                                                     aTCGTTCAGCTGAATAACTTCGTAATCCGCCAGTTCGTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCCACCGAACAGGCACACt                                                                                                                         <  1:126254/98‑1 (MQ=255)

                                                     aTCGTTCAGCTGAATAACTTCGTAATCCGCCAGTTCGTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCCACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTAcc                                                                                       <  1:1299537/132‑1 (MQ=255)

                                                     aTCGTTCAGCTGAATAACTTCGTAATCCGCCAGTTCGTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCCACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTAcc                                                                                       >  2:1299537/1‑132 (MQ=255)

                                                     aTCGTTCAGCTGAATAACTTCGTAATCCGCCAGTTCGTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCCACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTa                                                                                <  1:743305/139‑1 (MQ=255)

                                                     aTCGTTCAGCTGAATAACTTCGTAATCCGCCAGTTCGTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCCACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTa                                                                                >  1:853458/1‑139 (MQ=255)

                                                       cGTTCAGCTGAATAACTTCGTAATCCGCCAGTTCGTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCCACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATg                                                                              >  2:1148361/1‑139 (MQ=255)

                                                          tCAGCTGAATAACTTCGTAATCCGCCAGTTCGTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCCACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGtt                                                                           <  1:1262580/139‑1 (MQ=255)

                                                          tCAGCTGAATAACTTCGTAATCCGCCAGTTCGTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCCACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGtt                                                                           <  1:1205514/139‑1 (MQ=255)

                                                              cTGAATAACTTCGTAATCCGCCAGTTCGTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCCACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCg                                                                       >  1:1192241/1‑139 (MQ=255)

                                                              cTGAATAACTTCGTAATCCGCCAGTTCGTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCCACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCg                                                                       >  1:1375406/1‑139 (MQ=255)

                                                               tGAATAACTTCGTAATCCGCCAGTTCGTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCCACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGt                                                                      <  1:77534/139‑1 (MQ=255)

                                                               tGAATAACTTCGTAATCCGCCAGTTCGTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCCACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGt                                                                      <  2:1404173/139‑1 (MQ=255)

                                                                   tAACTTCGTAATCCGCCAGTTCGTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCCACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGtt                                                                           >  1:943593/1‑130 (MQ=255)

                                                                   tAACTTCGTAATCCGCCAGTTCGTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCCACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGtc                                                                           <  2:943593/130‑2 (MQ=255)

                                                                        tCGTAATCCGCCAGTTCGTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCCACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATaga                                                             <  1:1215495/139‑1 (MQ=255)

                                                                        tCGTAATCCGCCAGTTCGTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCCACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATaga                                                             >  1:208484/1‑139 (MQ=255)

                                                                                 gCCAGTTCGTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCCACCGAACAGGgaca                                                                                                                           <  2:715873/68‑1 (MQ=255)

                                                                                 gCCAGTTCGTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCCACCGAACAGGcaca                                                                                                                           >  1:715873/1‑68 (MQ=255)

                                                                                             aGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCCACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCg                                        <  1:998178/139‑1 (MQ=255)

                                                                                               tttACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCCAc                                                                                                                                       <  1:1281522/42‑1 (MQ=255)

                                                                                               tttACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCCAc                                                                                                                                       >  2:1281522/1‑42 (MQ=255)

                                                                                                ttACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCCACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCAt                                     >  1:575534/1‑139 (MQ=255)

                                                                                                         gCCAGATGATGGCGACGCAAAATATCCGCCACCGAACAgg                                                                                                                               >  2:911404/1‑40 (MQ=255)

                                                                                                         gCCAGATGATGGCGACGCAAAATATCCGCCACCGAACAgg                                                                                                                               <  1:911404/40‑1 (MQ=255)

                                                                                                                   ggCGACGCAAAATATCCGCCACCGAACAGGCACACTGGAAGTATTGCTGCa                                                                                                          >  1:966954/1‑51 (MQ=255)

                                                                                                                   ggCGACGCAAAATATCCGCCACCGAACAGGCACACTGGAAGTATTGCTGCa                                                                                                          <  2:966954/51‑1 (MQ=255)

                                                                                                                    gCGACGCAAAATATCCGCCACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCgg                   >  2:301116/1‑137 (MQ=255)

                                                                                                                    gCGACGCAAAATATCCGCCACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCgg                   <  1:301116/137‑1 (MQ=255)

                                                                                                                       aCGCAAAATATCCGCCACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCg                                        <  2:1087618/113‑1 (MQ=255)

                                                                                                                       aCGCAAAATATCCGCCACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCg                                        >  1:1087618/1‑113 (MQ=255)

                                                                                                                        cGCAAAATATCCGCCACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTa                                                                                >  2:728176/1‑72 (MQ=255)

                                                                                                                        cGCAAAATATCCGCCACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTa                                                                                <  1:728176/72‑1 (MQ=255)

                                                                                                                        cGCAAAATATCCGCCACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTg                                                     <  2:753061/99‑1 (MQ=255)

                                                                                                                        cGCAAAATATCCGCCACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTg                                                     >  1:753061/1‑99 (MQ=255)

                                                                                                                                   cGCCACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACcg  <  1:857013/139‑1 (MQ=255)

TGCTCATCGATCAGCACGCGCAGCAGTTCTGGAATCGCGATAGTTGGGTGGGTATCGTTCAGCTGAATAACTTCGTAATCCGCCAGTTCGTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCG  >  NC_000913/3551497‑3551766

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A