Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F1 I1 R1	77	35.1	1569012	96.6%	1515665	112.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	638,399	A→G	Y59H (TAC→CAC)	dsbG ←	thiol:disulfide interchange protein, periplasmic

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	638,399	0	A	G	100.0%	133.3 / NA	36	Y59H (TAC→CAC)	dsbG	thiol:disulfide interchange protein, periplasmic
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (18/18); total (18/18)

GTTCCATCCGTTGCCACATTTCGCGTCCGGCTGGTGCGTAAATTTCTTTTTCGATAAGTGTGTTACTCAGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGCGTGCTTACCATCTGGAGTCAGGTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTCAATCGCTTTTACTGGAGCAGG  >  NC_000913/638267‑638513

gTTCCATCCGTTGCCACATTTCGCGTCCGGCTGGTGCGTAAATTTCTTTTTCGATAAGTGTGTTACTCAGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGCGTGCTTACCATCTGGAGTCAGGTGGATGGt                                                                                                              <  1:671193/139‑1 (MQ=255)

 ttCCATCCGTTGCCACATTTCGCGTCCGGCTGGTGCGTAAATTTCTTTTTCGATAAGTGTGTTACTCAGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGCGTGCTTACCATCTGGAGTCAGGTGGATGGTg                                                                                                             >  1:225072/1‑139 (MQ=255)

           tGCCACATTTCGCGTCCGGCTGGTGCGTAAATTTCTTTTTCGATAAGTGTGTTACTCAGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGCGTGCTTACCATCTGGAGTCAGGTGGATGGTGACGCCCatat                                                                                                   >  1:655344/1‑139 (MQ=255)

                caTTTCGCGTCCGGCTGGTGCGTAAATTTCTTTTTCGATAAGTGTGTTACTCAGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGCGTGCTTACCATCTGGAGTCAGGTGGATGGTGACGCCCATATCCTGa                                                                                              <  2:114233/139‑1 (MQ=255)

                       cgTCCGGCTGGTGCGTAAATTTCTTTTTCGATAAGTGTGTTACTCAGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGCGTGCTTACCATCTGGAGTCAGGTGGa                                                                                                                  >  1:744254/1‑112 (MQ=255)

                       cgTCCGGCTGGTGCGTAAATTTCTTTTTCGATAAGTGTGTTACTCAGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGCGTGCTTACCATCTGGAGTCAGGTGGATGGTGACGCCCATATCCTGATACTTTc                                                                                       <  2:477840/139‑1 (MQ=255)

                             gCTGGTGCGTAAATTTCTTTTTCGATAAGTGTGTTACTCAGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGCGTGCTTACCATCTGGAGTCAGGTGGATGGTGACGCCCATATCCTGATACTTTCCGAGAt                                                                                 <  2:779526/139‑1 (MQ=255)

                                    cGTAAATTTCTTTTTCGATAAGTGTGTTACTCAGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGCGTGCTTACCATCTGGAGTCAGGTGGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCttt                                                                          >  2:281871/1‑139 (MQ=255)

                                                        aGTGTGTTACTCAGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGCGTGCTTACCATCTGGAGTCAGGTGGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGCATCGa                                                      <  1:281871/139‑1 (MQ=255)

                                                            tgtTACTCAGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGCGTGCTTACCATCTGGAGTCAGGTGGATGGTGACg                                                                                                          >  1:15820/1‑83 (MQ=255)

                                                            tgtTACTCAGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGCGTGCTTACCATCTGGAGTCAGGTGGATGGTGACg                                                                                                          <  2:15820/83‑1 (MQ=255)

                                                               tACTCAGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGCGTGCTTACCATCTGGAGTCAGGTGGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGCATCGAATGtttt                                               <  2:648002/139‑1 (MQ=255)

                                                                    aGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGCGTGCTTACCATCTGGAGTCAGGTGGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGCATCGAATGTTTtgat                                            <  1:674736/137‑1 (MQ=255)

                                                                    aGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGCGTGCTTACCATCTGGAGTCAGGTGGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGCATCGAATGTTTtgat                                            >  2:674736/1‑137 (MQ=255)

                                                                         ttCACCTTTCTCGTTGTACATGTAACCAGAGATAGCGTGCTTACCATCTGGAGTCAGGTGGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGCATCGAAt                                                    <  2:361554/124‑1 (MQ=255)

                                                                         ttCACCTTTCTCGTTGTACATGTAACCAGAGATAGCGTGCTTACCATCTGGAGTCAGGTGGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGCATCGAAt                                                    >  1:361554/1‑124 (MQ=255)

                                                                              cTTTCTCGTTGTACATGTAACCAGAGATAGCGTGCTTACCATCTGGAGTCAGGTGGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGCATCGAATGTTTTGATGATTGTAATGcc                                <  2:655344/139‑1 (MQ=255)

                                                                               tttCTCGTTGTACATGTAACCAGAGATAGCGTGCTTACCATCTGGAGTCAGGTGGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGCATCGAATGTTTTGATGATTGTAATGccc                               <  2:225072/139‑1 (MQ=255)

                                                                                           cATGTAACCAGAGATAGCGTGCTTACCATCTGGAGTCAGGTGGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTCAATc                   >  1:340404/1‑139 (MQ=255)

                                                                                                 aCCAGAGATAGCGTGCTTACCATCTGGAGTCAGGTGGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGCATCGAATGtt                                                 >  1:700479/1‑103 (MQ=255)

                                                                                                 aCCAGAGATAGCGTGCTTACCATCTGGAGTCAGGTGGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGCATCGAATGtt                                                 <  2:700479/103‑1 (MQ=255)

                                                                                                 aCCAGAGATAGCGTGCTTACCATCTGGAGTCAGGTGGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCATCCGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTCAATCGCtttt             >  1:124938/1‑139 (MQ=255)

                                                                                                      agaTAGCGTGCTTACCATCTGGAGTCAGGTGGATGGTGa                                                                                                            >  1:770034/1‑39 (MQ=255)

                                                                                                      agaTAGCGTGCTTACCATCTGGAGTCAGGTGGATGGTGa                                                                                                            <  2:770034/39‑1 (MQ=255)

                                                                                                          aGCGTGCTTACCATCTGGAGTCAGGTGGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTCAATCGCTTTTACTGGAGCa    >  2:304432/1‑139 (MQ=255)

                                                                                                            cGTGCTTACCATCTGGAGTCAGGTGGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTCAATCGCTTTTACTGGAGCAgg  >  2:582410/1‑139 (MQ=255)

                                                                                                                cTTACCATCTGGAGTCAGGTGGATGGTGACGCCCATATCCTGATACTTTCCgaga                                                                                  <  1:777974/55‑1 (MQ=255)

                                                                                                                cTTACCATCTGGAGTCAGGTGGATGGTGACGCCCATATCCTGATACTTTCCgaga                                                                                  >  2:777974/1‑55 (MQ=255)

                                                                                                                   aCCATCTGGAGTCAGGTGGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTCAATCGCTTTTACTGGa       >  2:257176/1‑127 (MQ=255)

                                                                                                                   aCCATCTGGAGTCAGGTGGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTCAATCGCTTTTACTGGa       <  1:257176/127‑1 (MQ=255)

                                                                                                                           gAGTCAGGTGGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCAt                                                                       <  2:686501/55‑1 (MQ=255)

                                                                                                                           gAGTCAGGTGGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCAt                                                                       >  1:686501/1‑55 (MQ=255)

                                                                                                                             gTCAGGTGGATGGTGACGCCCATATCCTGATACTTTCCgaga                                                                                  >  1:87329/1‑42 (MQ=255)

                                                                                                                             gTCAGGTGGATGGTGACGCCCATATCCTGATACTTTCCgaga                                                                                  <  2:87329/42‑1 (MQ=255)

                                                                                                                               cAGGTGGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTCAATCGCTTTTACTGGAg      <  1:432414/116‑1 (MQ=255)

                                                                                                                               cAGGTGGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTCAATCGCTTTTACTGGAg      >  2:432414/1‑116 (MQ=255)

GTTCCATCCGTTGCCACATTTCGCGTCCGGCTGGTGCGTAAATTTCTTTTTCGATAAGTGTGTTACTCAGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGCGTGCTTACCATCTGGAGTCAGGTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTCAATCGCTTTTACTGGAGCAGG  >  NC_000913/638267‑638513

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A