Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F1 I1 R1	77	35.1	1569012	96.6%	1515665	112.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,785,021	(G)_6→7	intergenic (‑6/‑239)	yibN ← / → gpmM	putative rhodanese‑related sulfurtransferase/phosphoglycero mutase III, cofactor‑independent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,785,015	1	.	G	100.0%	95.5 / NA	26	M1M (ATG→CTG) †	yibN	putative rhodanese‑related sulfurtransferase
Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (14/12); total (14/12)

CGAGTAATCACCTTCACTTTCGAGGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGATCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCAT‑GGGGGGTAACAACTCCCGACGTAGTGATTAAAAAAACCAGGAAAGGAGTATACCTGCGCGGTGCGGCAAATACAGCCAGCGCGTTAACTGGAATGCAATTTTGCGGGGCGCGACGAAAAAAAAGCGTAAAT  >  NC_000913/3784891‑3785146

cGAGTAATCACCTTCACTTTCGAGGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGATCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCAT‑gggggggtaa                                                                                                                           >  2:681839/1‑131 (MQ=255)

cGAGTAATCACCTTCACTTTCGAGGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGATCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCAT‑gggggggtaa                                                                                                                           <  1:681839/135‑5 (MQ=255)

  aGTAATCACCTTCACTTTCGAGGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGATCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCATGGGGGGGTAACAACTc                                                                                                                      >  1:228113/1‑139 (MQ=255)

                 tttCGAGGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGATCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCATGGGGGGGTAACAACTCCCGACGTAGTGATTa                                                                                                       >  1:782388/1‑139 (MQ=255)

                      aGGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGATCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCATGGGGGGGTAACAACTCCCGACGTAGTGATTaaaaaa                                                                                                  <  2:497164/139‑1 (MQ=255)

                           aGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGATCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCATGGGGGGGTAACAACTCCCGACGTAGTGATTaaa                                                                                                     <  2:335939/131‑1 (MQ=255)

                           aGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGATCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCATGGGGGGGTAACAACTCCCGACGTAGTGATTaaa                                                                                                     >  1:335939/1‑131 (MQ=255)

                              ctctTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGATCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCATGGGGGGGTAACAACTCCCGACGTAGTGATTAAAAAAACCAGGaa                                                                                          <  2:764964/139‑1 (MQ=255)

                              ctctTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGATCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCATGGGGGGGTAACAACTCCCGACGTAGTGATTAAAAAAACCAGGaa                                                                                          <  2:228113/139‑1 (MQ=255)

                                         aGTCACAAGAACCGCCACCAGTAACGCGATCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCATGGGGGGGTAACAACTCCCGACGTAGTGATTAAAAAAACCAGGAAAGGAGTATAcc                                                                               >  1:114044/1‑139 (MQ=255)

                                             acaAGAACCGCCACCAGTAACGCGATCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCATGGGGGGGTAACAACTCCCGACGTAGTGATTAAAAAAACCAGGAAAGGAGTATACCTgcg                                                                           <  1:532688/139‑1 (MQ=255)

                                                     cGCCACCAGTAACGCGATCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCATGGGGGGGTAACAACTCCCGACGTAGTGATTAAAAAAACCAGGAAAGGAGTATACCTGCGCGGTGCgg                                                                   >  2:313716/1‑139 (MQ=255)

                                                     cGCCACCAGTAACGCGATCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCATGGGGGGGTAACAACTCCCGACGTAGTGATTAAAAAAACCAGGAAAGGAGTATACCTGCGCGGTGCgg                                                                   <  2:782388/139‑1 (MQ=255)

                                                         accaGTAACGCGATCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCAT‑gggg                                                                                                                                 >  1:544295/1‑72 (MQ=255)

                                                         accaGTAACGCGATCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCAT‑gggg                                                                                                                                 <  2:544295/72‑1 (MQ=255)

                                                           caGTAACGCGATCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCATGGGGGGGTAACAACTCCCGACGTAGTGATTAAAAAAACCAGGAAAGGAGTATACCTGCGCGGTGCGGCAAATa                                                             <  1:313716/139‑1 (MQ=255)

                                                                   cgATCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCATGGGGGGGTAACAACTCCCGACGTAGTGATTAAAAAAACCAGGAAAGGAGTATACCTGCGCGGTGCGGCAAATAcagccagc                                                     <  1:262542/139‑1 (MQ=255)

                                                                      tCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCAT‑gggggggtaa                                                                                                                           <  1:30704/65‑5 (MQ=255)

                                                                      tCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCAT‑gggggggtaa                                                                                                                           >  2:30704/1‑61 (MQ=255)

                                                                              aTACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCATGGGGGGGTAACAACTCCCGACGTAGTGATTAAAAAAACCAGGAAAGGAGTATACCTGCGCGGTGCGGCAAATACAGCCAGCGCGTTAACTgg                                          >  1:70535/1‑139 (MQ=255)

                                                                                aCTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCATGGGGGGGTAACAACTCCCGACGTAGTGATTAAAAAAACCAGGAAAGGAGTATACCTGCGCGGTGCGGCAAATACAGCCAGCGCGTTAACTGGaa                                        >  1:696541/1‑139 (MQ=255)

                                                                                         gggATGACGGCCAACAAATTGCATAATTTCTTGCATGGGGGGGTAACAACTCCCGACGTAGTGATTAAAAAAACCAGGAAAGGAGTATACCTGCGCGGTGCGGCAAATACAGCCAGCGCGTTAACTGGAATGCAAtttt                               >  1:612202/1‑139 (MQ=255)

                                                                                           gATGACGGCCAACAAATTGCATAATTTCTTGCATGGGGGGGTAACAACTCCCGACGTAGTGATTAAAAAAACCAGGAAAGGAGTATACCTGCGCGGTGCGGCAAATACAGCCAGCGCGTTAACTGGAATGCAATTTTGc                             >  1:412094/1‑139 (MQ=255)

                                                                                              gACGGCCAACAAATTGCATAATTTCTTGCATGGGGGGGTAACAACTCCCGACGTAGTGATTAAAAAAACCAGGAAAGGAGTATACCTGCGCGGTGCGGCAAATACAGCCAGCGCGTTAACTGGAATGCAATTTTGCggg                          >  1:55030/1‑139 (MQ=255)

                                                                                              gACGGCCAACAAATTGCATAATTTCTTGCAT‑gggggggtaa                                                                                                                           <  2:438452/41‑5 (MQ=255)

                                                                                              gACGGCCAACAAATTGCATAATTTCTTGCAT‑gggggggtaa                                                                                                                           >  1:438452/1‑37 (MQ=255)

                                                                                               aCGGCCAACAAATTGCATAATTTCTTGCATGGGGGGGTAACAACTCCCGACGTAGTGATTAAAAAAACCAGGAAAGGAGTATACCTGCGCGGTGCGGCAAATACAGCCAGCGCGTTAACTGGAATGCAATTTTGCgggg                         >  1:102475/1‑139 (MQ=255)

                                                                                                cGGCCAACAAATTGCATAATTTCTTGCATGGGGGGGTAACAACTCCCGACGTAGTGATTAAAAAAACCAGGAAAGGAGTATACCTGCGCGGTGCGGCAAATAcagccagc                                                     <  2:153036/110‑1 (MQ=255)

                                                                                                cGGCCAACAAATTGCATAATTTCTTGCATGGGGGGGTAACAACTCCCGACGTAGTGATTAAAAAAACCAGGAAAGGAGTATACCTGCGCGGTGCGGCAAATAcagccagc                                                     >  1:153036/1‑110 (MQ=255)

                                                                                                cGGCCAACAAATTGCATAATTTCTTGCATGGGGGGGTAACAACTCCCGACGTAGTGATTAAAAAAACCAGGAAAGGAGTATACCTGCGCGGTGCGGCAAATACAGCCAGCGCGTTAACTGGAATGCAATTTTGCGGGgc                        >  2:305722/1‑139 (MQ=255)

                                                                                                cGGCCAACAAATTGCATAATTTCTTGCATGGGGGGGTAACAACTCCCGACGTAGTGATTAAAAAAACCAGGAAAGGAGTATACCTGCGCGGTGCGGCAAATACAGCCAGCGCGTTAACTGGAATGCAATTTTGCGGGgc                        >  2:575402/1‑139 (MQ=255)

                                                                                                    caacaaATTGCATAATTTCTTGCATGGGGGGGTAACAACTCCCGACGTAGTGATTAAAAAAACCAGGAAAGGAGTATACCTGCGCGGTGCGGCAAATACAGCCAGCGCGTTAACTGGAATGCAATTTTGCGGGGCGcga                    <  2:70535/139‑1 (MQ=255)

                                                                                                                  aTTTCTTGCATGGGGGGGTAACAACTCCCGACGTAGTGATTAAAAAAACCAGGAAAGGAGTATACCTGCGCGGTGCGGCAAATACAGCCAGCGCGTTAACTGGAATGCAATTTTGCGGGGCGCGACGAAAAAAAAGCGt      <  2:612202/139‑1 (MQ=255)

                                                                                                                      cTTGCATGGGGGGGTAACAACTCCCGACGTAGTGATTAAAAAAACCAGGAAAGGAGTATACCTGCGCGGTGCGGCAAATACAGCCAGCGCGTTAACTGGAATGCAATTTTGCGGGGCGCGACGAAAAAAAAGCGTAAAt  <  2:55030/139‑1 (MQ=255)

CGAGTAATCACCTTCACTTTCGAGGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGATCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCAT‑GGGGGGTAACAACTCCCGACGTAGTGATTAAAAAAACCAGGAAAGGAGTATACCTGCGCGGTGCGGCAAATACAGCCAGCGCGTTAACTGGAATGCAATTTTGCGGGGCGCGACGAAAAAAAAGCGTAAAT  >  NC_000913/3784891‑3785146

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A