Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A11 F1 I2 R1
|
59 |
52.6 |
2411032 |
94.1% |
2268781 |
113.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,762,338 |
T→C |
T59A (ACG→GCG) |
sufC ← |
SufBCD Fe‑S cluster assembly scaffold protein, ATP‑binding protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,762,338 | 0 | T | C | 100.0%
| 107.5
/ NA
| 29 | T59A (ACG→GCG) | sufC | SufBCD Fe‑S cluster assembly scaffold protein, ATP‑binding protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (17/12); total (17/12) |
CGTTTGCAGGAAAAACTGGTTACTGACACCTGGAATCTCCACCGGATACTGGAAGGCCATAAAGATGCCTTCGCCCGCGCGATCTTCCGGCGACAGCGCAAGCAAATCTTTGCCTTTGAACTCAACCGTGCCGCCCGTCACTTCATAATCTTCTCGCCCGGCAAGCGTTGCCGATAAGGTACTTTTGCCCGAACCGTTTGGCCCCATAATGGCGTGAACTTCGCCGGGATGAACGTCGAGGCTTAATCCGCGCAGGATAGCT > NC_000913/1762210‑1762471
|
cGTTTGCAGGAAAAACTGGTTACTGACACCTGGAATCTCCACCGGATACTGGAAGGCCATAAAGATGCCTTCGCCCGCGCGATCTTCCGGCGACAGCGCAAGCAAATCTTTGCCTTTGAACTCAACCGCGCCGCCCGTc < 1:907940/139‑1 (MQ=255)
cGTTTGCAGGAAAAACTGGTTACTGACACCTGGAATCTCCACCGGATACTGGAAGGCCATAAAGATGCCTTCGCCAGCGCGATCTTCCGGCGACAGCGCAAGCAAATCTTTGCCTTTGAACTCAACCGCGCCGCCCGTc > 2:776305/1‑139 (MQ=255)
ttGCAGGAAAAACTGGTTACTGACACCTGGAATCTCCACCGGATACTGGAAGGCCATAAAGATGCCTTCGCCCGCGCGATCTTCCGGCGACAGCGCAAGCAAATCTTTGCCTTTGAACTCAACCGCGCCGCCCGTCACt > 2:792001/1‑139 (MQ=255)
ccTGGAATCTCCACCGGATACTGGAAGGCCATAAAGATGCCTTCGCCCGCGCGATCTTCCGGCGACAGCGCAAGCAAATCTTTGCCTTTGAACTCAACCGCGCCGCCCGt > 1:602645/1‑110 (MQ=255)
ccTGGAATCTCCACCGGATACTGGAAGGCCATAAAGATGCCTTCGCCCGCGCGATCTTCCGGCGACAGCGCAAGCAAATCTTTGCCTTTGAACTCAACCGCGCCGCCCGt < 2:602645/110‑1 (MQ=255)
cTGGAATCTCCACCGGATACTGGAAGGCCATAAAGATGCCTTCGCCCGCGCGATCTTCCGGCGACAGCGCAAGCAAATCTTTGCCTTTGAACTCAACCGCGCCGCCCGTCACTTCATAATCTTCTCGCCCGGCAAGCGt < 2:964819/139‑1 (MQ=255)
ggCCATAAAGATGCCTTCGCCCGCGCGATCTTCCGGCGACAGCGCAAGCAAATCTTTGCCTTTGAACTCAACCGCGCCGCCCGTc > 1:288239/1‑85 (MQ=255)
ggCCATAAAGATGCCTTCGCCCGCGCGATCTTCCGGCGACAGCGCAAGCAAATCTTTGCCTTTGAACTCAACCGCGCCGCCCGTc < 2:288239/85‑1 (MQ=255)
ggCCATAAAGATGCCTTCGCCCGCGCGATCTTCCGGCGACAGCGCAAGCAAATCTTTGCCTTTGAACTCAACCGCGCCGCCCGTCACTTCATAATCTTCTCGCCCGGCAAGCGTTGCCGATAAGGTACTTTTGCCCGaa > 1:510155/1‑139 (MQ=255)
ccATAAAGATGCCTTCGCCCGCGCGATCTTCCGGCGACAGCGCAAGCAAATCTTTGCCTTTGAACTCAACCGCGCCGCCCGTCACTTCATAATCTTCTCGCCCGGCAAGCGTTGCCGATAAGGTACTTTTGCCCGAAcc > 2:739099/1‑139 (MQ=255)
ccATAAAGATGCCTTCGCCCGCGCGATCTTCCGGCGACAGCGCAAGCAAATCTTTGCCTTTGAACTCAACCGCGCCGCCCGTCACTTCATAATCTTCTCGCCCGGCAAGCGTTGCCGATAAGGTACTTTTGCCCGAAcc > 1:872846/1‑139 (MQ=255)
tAAAGATGCCTTCGCCCGCGCGATCTTCCGGCGACAGCGCAAGCAAATCTTTGCCTTTGAACTCAACCGCGCCGCCCGTCACTTCATAATCTTCTCGCCCGGCAAGCGTTGCCGATAAGGTACTTTTGCCCGAACCGtt < 2:872846/139‑1 (MQ=255)
gCCTTCGCCCGCGCGATCTTCCGGCGACAGCGCAAGCAAATCTTTGCCTTTGAACTCAACCGCGCCGCCCGTCACTTCATAATCTTCTCGCCCGGCAAGCGTTGCCGATAAGGTACTTTTGCCCGAACCGTTTGGcccc > 1:456520/1‑139 (MQ=255)
ccTTCGCCCGCGCGATCTTCCGGCGACAGCGCAAGCAAATCTTTGCCTTTGAACTCAACCGCGCCGCCCGTCACTTCATAATCTTCTCGCCCGGCAAGCGTTGCCGATAAGGTACTTTTGCCCGAACCGTTTgg > 1:394399/1‑134 (MQ=255)
ccTTCGCCCGCGCGATCTTCCGGCGACAGCGCAAGCAAATCTTTGCCTTTGAACTCAACCGCGCCGCCCGTCACTTCATAATCTTCTCGCCCGGCAAGCGTTGCCGATAAGGTACTTTTGCCCGAACCGTTTgg < 2:394399/134‑1 (MQ=255)
cGCCCGCGCGATCTTCCGGCGACAGCGCAAGCAAATCTTTGCCTTTGAACTCAACCGCGCCGCCCGTCACTTCATAATCTTCTCGCCCGGCAAGCGTTGCCGATAAGGTACTTTTGCCCGAACCGTTTGGCCCCATAAt > 2:119865/1‑139 (MQ=255)
gCCCGCGCGATCTTCCGGCGACAGCGCAAGCAAATCTTTGCCTTTGAACTCAACCGCGCCGCCCGTCACTTCATAATCTTCTCGCCCGGCAAGCGTTGCCGATAAGGTACTTTTGCCCGAACCGTTTGGCCCCATAATg > 2:597636/1‑139 (MQ=255)
cccGCGCGATCTTCCGGCGACAGCGCAAGCAAATCTTTGCCTTTGAACTCAACCGCGCCGCCCGTCACTTCATAATCTTCTCGCCCGGCAAGCGTTGc > 1:888721/1‑98 (MQ=255)
cccGCGCGATCTTCCGGCGACAGCGCAAGCAAATCTTTGCCTTTGAACTCAACCGCGCCGCCCGTCACTTCATAATCTTCTCGCCCGGCAAGCGTTGc < 2:888721/98‑1 (MQ=255)
aGCGCAAGCAAATCTTTGCCTTTGAACTCAACCGCGCCGCCCGTCACTTCATAATCTTCTCGCCCGGCAAGCGTTGCCGATAAGGTACTTTTGCCCGAACCGTTTGGCCCCATAATGGCGTGAACTTCGCCGGGATGaa > 2:961229/1‑139 (MQ=255)
cgcAAGCAAATCTTTGCCTTTGAACTCAACCGCGCCGCCCGTCACTTCATAATCTTCTCGCCCGGCAAGCGTTGCCg < 2:335974/77‑1 (MQ=255)
cgcAAGCAAATCTTTGCCTTTGAACTCAACCGCGCCGCCCGTCACTTCATAATCTTCTCGCCCGGCAAGCGTTGCCg > 1:335974/1‑77 (MQ=255)
caagcaaATCTTTGCCTTTGAACTCAACCGCGCCGCCCGTCACTTCATAATCTTCTCGCCCGGCAAGCGTTGCCGATAAGGTACTTTTGCCCGAACCGTTTGGCCCCATAATGGCGTGAACTTCGCCGGGATGAACGTc < 1:961229/139‑1 (MQ=255)
gCCTTTGAACTCAACCGCGCCGCCCGTCACTTCATAATCTTCTCGCCCGGCAAGCGTTGCCGATAAGGTACTTTTGCCCGAACCGTTTGGCCCCATAATGGCGTGAACTTCGCCGGGATGAACGTCGAGGCTTAATCcg > 1:382408/1‑139 (MQ=255)
cTTTGAACTCAACCGCGCCGCCCGTCACTTCATAGTCTTCTCGCCCGGCAAGCGTTGCCGATAAGGTACTTTTGCCCGAACCGTTTGGCCCCATAATGGCGTGAACTTCGCCGGGATGAACGTCGAGGCTTAATCcgcg < 1:776305/139‑1 (MQ=255)
gAACTCAACCGCGCCGCCCGTCACTTCATAATCTTCTCGCCCGGCAAGCGTTGCCGATAAGGTACTTTTGCCCGAACCGTTTGGCCCCATAATGGCGTGAACTTCGCCGGGATGAACGTCGAGGCTTAATCCGCGCAgg < 2:456520/139‑1 (MQ=255)
aaCCGCGCCGCCCGTCACTTCATAATCTTCTCGCCCGGCAAGCGTTGCCGATAAGGTACTTTTGCCCGAACCGTTTGGCCCCATAATGGCGTGAACTTCGCCGGGATGAACGTCGAGGCTTAATCCGCGCAGGATAGCt > 1:816023/1‑139 (MQ=255)
ccGCGCCGCCCGTCACTTCATAATCTTCTCGCCCGGCAAGCGTTGCCGATAAGGTACTTTTGCCCGaa > 2:765324/1‑68 (MQ=255)
ccGCGCCGCCCGTCACTTCATAATCTTCTCGCCCGGCAAGCGTTGCCGATAAGGTACTTTTGCCCGaa < 1:765324/68‑1 (MQ=255)
|
CGTTTGCAGGAAAAACTGGTTACTGACACCTGGAATCTCCACCGGATACTGGAAGGCCATAAAGATGCCTTCGCCCGCGCGATCTTCCGGCGACAGCGCAAGCAAATCTTTGCCTTTGAACTCAACCGTGCCGCCCGTCACTTCATAATCTTCTCGCCCGGCAAGCGTTGCCGATAAGGTACTTTTGCCCGAACCGTTTGGCCCCATAATGGCGTGAACTTCGCCGGGATGAACGTCGAGGCTTAATCCGCGCAGGATAGCT > NC_000913/1762210‑1762471
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
