Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F1 I2 R1	59	52.6	2411032	94.1%	2268781	113.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,297,084	C→T	Q187Q (CAG→CAA)	ccmA ←	heme export ABC transporter ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,297,084	0	C	T	100.0%	164.2 / NA	47	Q187Q (CAG→CAA)	ccmA	heme export ABC transporter ATPase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (18/29); total (18/29)

GGTTGGCGATTTCGGCGCTATGGCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAACGTGGCACGGGTC  >  NC_000913/2296955‑2297213

ggTTGGCGATTTCGGCGCTATGGCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGTTGGTGGGTa                                                                                                                          <  1:544444/139‑1 (MQ=255)

  ttGGCGATTTCGGCGCTATGGCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGTTGGTGGGTAGt                                                                                                                        <  1:970672/139‑1 (MQ=255)

   tGGCGATTTCGGCGCTATGGCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGTTGGTGGGTAGTc                                                                                                                       >  1:888745/1‑139 (MQ=255)

          ttCGGCGCTATGGCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGTTGGTGGGTAGTCAGAATca                                                                                                                <  2:360902/139‑1 (MQ=255)

             ggCGCTATGGCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGTTGGTGGGTAGTCAGAATCACaa                                                                                                             <  1:639243/139‑1 (MQ=255)

                    tGGCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGTTGGTGGGTAGTCAGAATCACAATcccccc                                                                                                      <  1:127594/139‑1 (MQ=255)

                    tGGCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGTTGGTGGGTAGTCAGAATCACAATcccccc                                                                                                      <  2:888745/139‑1 (MQ=255)

                     ggCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGTTGGTGGGTAGTCAGAATCACAATcccccct                                                                                                     >  2:99895/1‑138 (MQ=255)

                     ggCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGTTGGTGGGTAGTCAGAATCACAATccccccc                                                                                                     >  2:1055883/1‑139 (MQ=255)

                      gCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGTTGGTGGGTAGTCAGAATCACAATCCCCCCCt                                                                                                    <  1:1099924/139‑1 (MQ=255)

                           aCGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGTTGGTGGGTAGTCAGAATCACAATCCCCCCCTGCTcc                                                                                               <  1:818180/139‑1 (MQ=255)

                           aCGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGTTGGTGGGTAGTCAGAATCACAATCCCCCCCTGCTcc                                                                                               >  2:597355/1‑139 (MQ=255)

                              cTACACGCAGCTCAAGACTGAAAATGCGCCAGAACATCATGCGGCCATCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGTTGGTGGGTAGTCAGAATCACaa                                                                                                             <  1:728417/122‑1 (MQ=255)

                              cTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGTTGGTGGGTAGTCAGAATCACaa                                                                                                             >  2:728417/1‑122 (MQ=255)

                                       gCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGTTGGTGGGTAGTCAGAATCACAATCCCCCCCTGCTCCGTATGCTGCGcc                                                                                   >  1:302802/1‑139 (MQ=255)

                                            aGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGTTGGTGGGTAGTCAGAATCACAATCCCCCCCTGCTCCGTATGCTGCGCCATACg                                                                              <  1:1055883/139‑1 (MQ=255)

                                               cGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGAAACGTTGAGCGGTTGGTGGGTAGTCAGAATCACAATCCCCCCCTGCTCCGTATGCTGCGCCATACGCTg                                                                           <  1:648764/139‑1 (MQ=255)

                                                  aaaaTGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGTTGGTGGGTAGTCAGAATCACAATCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGt                                                                        >  2:154700/1‑139 (MQ=255)

                                                      tGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGTTGGTGGGTAGTCAGAATCACAATCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGa                                                                    <  1:587050/139‑1 (MQ=255)

                                                          ccAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGTTGGTGGGTAGTCAGAATCACAATCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGAt                                                                <  1:597355/139‑1 (MQ=255)

                                                            aGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGTTGGTGGGTAGTCAGAATCACAATCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCg                                                              >  2:806404/1‑139 (MQ=255)

                                                              aaCATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGTTGGTGGGTAGTCAGAATCACAATCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGac                                                            <  1:631505/139‑1 (MQ=255)

                                                                       cGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGTTGGTGGGTAGTCAGAATCACAATCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGaca                                                           <  1:1032402/131‑1 (MQ=255)

                                                                       cGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGTTGGTGGGTAGTCAGAATCACAATCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGaca                                                           >  2:1032402/1‑131 (MQ=255)

                                                                       cGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGTTGGTGGGTAGTCAGAATCACAATCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAAc                                                   >  2:478412/1‑139 (MQ=255)

                                                                          cccTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGTTGGTGGGTAGTCAGAATCACAATCCCCCCCTGCTCCGTATGCTGCGCCa                                                                                  <  1:250354/105‑1 (MQ=255)

                                                                          cccTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGTTGGTGGGTAGTCAGAATCACAATCACCCCCTGCTCCGTATGCTGCGCCa                                                                                  >  2:250354/1‑105 (MQ=255)

                                                                              cGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGTTGGTGGGTAGTCAGAATCACAATCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATc                                            <  2:217854/139‑1 (MQ=255)

                                                                                tttGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGTTGGTGGGTAGTCAGAATCACAATCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATcgc                                          >  2:1127852/1‑139 (MQ=255)

                                                                                         gTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGTTGGTGGGTAGTCAGAATCACAATCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAgg                                 >  1:100847/1‑139 (MQ=255)

                                                                                          tGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGTTGGTGGGTAGTCAGAATCACAATCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGc                                >  2:1105597/1‑139 (MQ=255)

                                                                                           gAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGTTGGTGGGTAGTCAGAATCACAATCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCt                               <  2:302802/139‑1 (MQ=255)

                                                                                                 cggcgAATTTTACTTTCAGCAACGTTGAGCGGTTGGTGGGTAGTCAGAATCACAATCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGa                         <  2:449774/139‑1 (MQ=255)

                                                                                                 cggcgAATTTTACTTTCAGCAACGTTGAGCGGTTGGTGGGTAGTCAGAATCACAATCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGa                         <  2:15379/139‑1 (MQ=255)

                                                                                                 cggcgAATTTTACTTTCAGCAACGTTGAGCGGTTGGTGGGTAGTCAGAATCACAATCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGa                         <  1:868037/139‑1 (MQ=255)

                                                                                                       aTTTTACTTTCAGCAACGTTGAGCGGTTGGTGGGTAGTCAGAATCACAAT‑CCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCa                  <  1:99895/139‑1 (MQ=255)

                                                                                                                tCAGCAACGTTGAGCGGTTGGTGGGTAGTCAGAATCACAATCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATaa               <  1:1028955/134‑1 (MQ=255)

                                                                                                                tCAGCAACGTTGAGCGGTTGGTGGGTAGTCAGAATCACAATCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATaa               >  2:1028955/1‑134 (MQ=255)

                                                                                                                tCAGCAACGTTGAGCGGTTGGTGGGTAGTCAGAATCACAATCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAACGTgg          >  1:635319/1‑139 (MQ=255)

                                                                                                                 cAGCAACGTTGAGCGGTTGGTGGGTAGTCAGAATCACAATCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAACGTGGc         <  1:806404/139‑1 (MQ=255)

                                                                                                                  aGCAACGTTGAGCGGTTGGTGGGTAGTCAGAATCACAATCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGa                         >  1:951186/1‑122 (MQ=255)

                                                                                                                  aGCAACGTTGAGCGGTTGGTGGGTAGTCAGAATCACAATCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGa                         <  2:951186/122‑1 (MQ=255)

                                                                                                                     aaCGTTGAGCGGTTGGTGGGTAGTCAGAATCACAATCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAACGTGGCACgg     <  1:1105597/139‑1 (MQ=255)

                                                                                                                      aCGTTGAGCGGTTGGTGGGTAGTCAGAATCACAATCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAACGTGGCACggg    <  2:100847/139‑1 (MQ=255)

                                                                                                                        gTTGAGCGGTTGGTGGGTAGTCAGAATCACAATCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAACGTGGCACGGGTc  <  1:478412/139‑1 (MQ=255)

                                                                                                                            aGCGGTTGGTGGGTAGTCAGAATCACAATCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAg                                                                     >  2:33510/1‑68 (MQ=255)

                                                                                                                            aGCGGTTGGTGGGTAGTCAGAATCACAATCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAg                                                                     <  1:33510/68‑1 (MQ=255)

GGTTGGCGATTTCGGCGCTATGGCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAACGTGGCACGGGTC  >  NC_000913/2296955‑2297213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A