Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F1 I2 R1
|
58 |
36.1 |
1832140 |
90.7% |
1661750 |
105.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,271,600 |
(C)5→6 |
coding (171/1146 nt) |
garK ← |
glycerate kinase I |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,271,595 | 1 | . | C | 100.0%
| 95.1
/ NA
| 26 | A59G (GCT→GGT) | garK | glycerate kinase I |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (13/13); total (13/13) |
CCGCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAG‑CCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAATTTCCCGAAATCCTTTTTCTATCGCCTGCGCA > NC_000913/3271467‑3271714
|
ccGCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAG‑cccccctggg < 2:182610/139‑6 (MQ=255)
gTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAGCGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATCCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTGCAGCCCCCCTGGGTGGCTg < 1:482530/139‑1 (MQ=255)
ccACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTcc > 2:563475/1‑139 (MQ=255)
tGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCg > 1:261458/1‑139 (MQ=255)
cgccATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTc > 1:42113/1‑139 (MQ=255)
cgccATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTc > 2:405568/1‑139 (MQ=255)
aCGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCaa > 1:833332/1‑139 (MQ=255)
cgcgGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAAc < 2:833332/139‑1 (MQ=255)
cgcgGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAAc < 2:261458/139‑1 (MQ=255)
gcgGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAatc > 2:422806/1‑103 (MQ=255)
gcgGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAatc < 1:422806/103‑1 (MQ=255)
ttttGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGaa < 2:172118/139‑1 (MQ=255)
ttttGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGaa < 1:146036/139‑1 (MQ=255)
aTCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAAc < 1:563475/139‑1 (MQ=255)
cGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGt > 1:133791/1‑139 (MQ=255)
gCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCAcc < 2:302708/101‑1 (MQ=255)
gCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCAcc > 1:302708/1‑101 (MQ=255)
ttCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAATTTCCCGAAAt < 2:133791/139‑1 (MQ=255)
tctcGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAATTTCCCGAAATc > 2:602365/1‑139 (MQ=255)
ctcGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAATTTCCCGAAATcc < 2:344983/139‑1 (MQ=255)
cGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAAc < 1:738938/92‑1 (MQ=255)
cGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAAc > 2:738938/1‑92 (MQ=255)
aGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCa < 1:771410/85‑1 (MQ=255)
aGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCa > 2:771410/1‑85 (MQ=255)
aCCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAATTTCCCGAAATCCTTTTTCTATCGCCTGCGCa > 2:288748/1‑139 (MQ=255)
gCGTGACGTTCAGCCCCCCTGGGTGGCTGTAATCATCGCTTCCa < 2:176668/44‑1 (MQ=255)
gCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCa > 1:176668/1‑44 (MQ=38)
|
CCGCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAG‑CCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAATTTCCCGAAATCCTTTTTCTATCGCCTGCGCA > NC_000913/3271467‑3271714
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
