Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F1 I2 R1	58	36.1	1832140	90.7%	1661750	105.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,182,791	A→G	D516G (GAC→GGC)	rpoB →	RNA polymerase, beta subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,182,791	0	A	G	100.0%	142.1 / NA	38	D516G (GAC→GGC)	rpoB	RNA polymerase, beta subunit
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (20/18); total (20/18)

GCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGA  >  NC_000913/4182660‑4182901

gCGTGCGGTGAAAGAGCATCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGGCCAGaac                                                                                                         >  2:571262/1‑139 (MQ=255)

 cGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGACAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGGCCAGaaca                                                                                                        >  2:899749/1‑139 (MQ=255)

   tGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGGCCAGaacaac                                                                                                      >  2:411905/1‑139 (MQ=255)

     cGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGGCCAGAACAAccc                                                                                                    >  2:236320/1‑139 (MQ=255)

      ggTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGGCCAGAACAACCCg                                                                                                   <  2:898736/139‑1 (MQ=255)

      ggTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGGCCAGAACAACCCg                                                                                                   <  1:236320/139‑1 (MQ=255)

      ggTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGGCCAGAACAACCCg                                                                                                   <  1:95317/139‑1 (MQ=255)

                  tctgtctCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGGCCAGAACAACCCg                                                                                                   >  2:515705/1‑127 (MQ=255)

                  tctgtctCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGGCCAGAACAACCCg                                                                                                   <  1:515705/127‑1 (MQ=255)

                                aTCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGGCCAGAACAACCCGCTGTCTGAGATTACGcaca                                                                                <  1:134751/132‑1 (MQ=255)

                                aTCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGGCCAGAACAACCCGCTGTCTGAGATTACGcaca                                                                                >  2:134751/1‑132 (MQ=255)

                                      aTACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGGCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTAtctc                                                                   <  2:553189/139‑1 (MQ=255)

                                               tGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGGCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCgg                                                          >  1:589999/1‑139 (MQ=255)

                                               tGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGGCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCgg                                                          >  2:3783/1‑139 (MQ=255)

                                                 ccACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGGCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGcc                                                        >  2:531271/1‑139 (MQ=255)

                                                    caGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGGCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAg                                                     >  2:778867/1‑139 (MQ=255)

                                                    caGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGGCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAg                                                     >  2:699548/1‑139 (MQ=255)

                                                    caGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGGCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAg                                                     <  1:411905/139‑1 (MQ=255)

                                                          atGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGGCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTc                                               <  1:3783/139‑1 (MQ=255)

                                                              tCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGGCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGAc                                           <  2:482996/139‑1 (MQ=255)

                                                                 aCGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGGCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGt                                                >  1:716068/1‑131 (MQ=255)

                                                                 aCGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGGCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGt                                                <  2:716068/131‑1 (MQ=255)

                                                                 aCGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGGCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCg                                        >  1:526104/1‑139 (MQ=255)

                                                                 aCGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGGCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCg                                        >  1:100923/1‑139 (MQ=255)

                                                                  cGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGGCCAGAACAACCCGCTGTCTGAGAt                                                                                        >  1:93716/1‑90 (MQ=255)

                                                                  cGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGGCCAGAACAACCCGCTGTCTGAGAt                                                                                        <  2:93716/90‑1 (MQ=255)

                                                                   gCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGGCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGAcc                                          >  2:269079/1‑135 (MQ=255)

                                                                   gCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGGCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGAcc                                          <  1:269079/135‑1 (MQ=255)

                                                                       aGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGGCCAGAACAACCCGCTGTCTGAGATTa                                                                                      <  2:202404/87‑1 (MQ=255)

                                                                       aGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGGCCAGAACAACCCGCTGTCTGAGATTa                                                                                      >  1:202404/1‑87 (MQ=255)

                                                                                                gTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGGCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTa         >  2:77055/1‑139 (MQ=255)

                                                                                                   cttcGGTTCCAGCCAGCTGTCTCAGTTTATGGGCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGc                               <  1:90835/114‑1 (MQ=255)

                                                                                                   cttcGGTTCCAGCCAGCTGTCTCAGTTTATGGGCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGc                               >  2:90835/1‑114 (MQ=255)

                                                                                                    ttcGGTTCCAGCCAGCTGTCTCAGTTTATGGGCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTAGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTCCAcc     <  1:531271/139‑1 (MQ=255)

                                                                                                       ggTTCCAGCCATCTGTATCAGTTTATGGGCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGa  <  1:899749/139‑1 (MQ=255)

                                                                                                       ggTTCCAGCCAGCTGTCTCAGTTTATGGGCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGa  <  2:526104/139‑1 (MQ=255)

                                                                                                              gccagcTGTCTCAGTTTATGGGCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGt                   <  2:338553/115‑1 (MQ=255)

                                                                                                              gccagcTGTCTCAGTTTATGGGCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGt                   >  1:338553/1‑115 (MQ=255)

GCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGA  >  NC_000913/4182660‑4182901

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A