Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F1 I1 R1	64	69.7	3300380	93.4%	3082554	111.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,342,683	T→C	G137G (GGT→GGC)	kdsC →	3‑deoxy‑D‑manno‑octulosonate 8‑phosphate phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,342,683	0	T	C	84.2%	199.5 / 23.7	76	G137G (GGT→GGC)	kdsC	3‑deoxy‑D‑manno‑octulosonate 8‑phosphate phosphatase
Reads supporting (aligned to +/- strand): ref base T (6/6); new base C (34/30); total (40/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

ACTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTATGTCGGCGATGATCTCATCGACTGGCCGGTAATGGAAAAAGTGGGTTTAAGCGTCGCCGTGGCCGATGCGCATCCACTGTTGATCCCGCGCGCCGATTACGTGACGCGCATTGCTGGCGGTCGTGGCGCAGTGCGCGAAGTTTGCGACTTATTACTCCTGGCGCAGGGCAAACTGGATGAAG  >  NC_000913/3342549‑3342819

actcactTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTATGTCGGCGATGATCTCATCGACTGGCCGGTAATGGAAAAAGTGGGCTTaa                                                                                                                                      >  2:706215/1‑139 (MQ=255)

   cactTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTATGTCGGCGATGATCTCATCGACTGGCCGGTAATGGAAAAAGTGGGCTTAAGCg                                                                                                                                   <  1:158939/139‑1 (MQ=255)

        gTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTATGTCGGCGATGATCTCATCGACTGGCCGGTAATGGAAAAAGTGGGCTTAAGCg                                                                                                                                   >  1:825950/1‑134 (MQ=255)

        gTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCGGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTATGTCGGCGATGATCTCATCGACTGGCCGGTAATGGAAAAAGTGGGCTTAAGCg                                                                                                                                   <  2:825950/134‑1 (MQ=255)

         tATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTATGTCGGCGATGATCTCATCGACTGGCCGGTAATGGAAAAAGTGGGCTTAAGCg                                                                                                                                   >  2:1608976/1‑133 (MQ=255)

         tATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTATGTCGGCGATGATCTCATCGACTGGCCGGTAATGGAAAAAGTGGGCTTAAGCg                                                                                                                                   <  1:1608976/133‑1 (MQ=255)

           tCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTATGTCGGCGATGATCTCATCGACTGGCCGGTAATGGAAAAAGTGGGCTTAAGCGTCGCCGTg                                                                                                                           >  2:906002/1‑139 (MQ=255)

             aGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTATGTCGGCGATGATCTCATCGACTGGCCGGTAATGGAAAAAGTGGGCTTAAGCGTCGCCGTGGc                                                                                                                         <  2:22505/139‑1 (MQ=255)

              ggggCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTATGTCGGCGATGATCTCATCGACTGGCCGGTAATGGAAAAAGTGGGCTTAAGCGTCGCCGTGGcc                                                                                                                        >  2:67561/1‑139 (MQ=255)

              ggggCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTATGTCGGCGATGATCTCATCGACTGGCCGGTAATGGAAAAAGTGGGCTTAAGCGTCGCCGTGGcc                                                                                                                        >  1:817568/1‑139 (MQ=255)

                ggCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTATGTCGGCGATGATCTCATCGACTGGCCGGTAATGGAAAAAGTGGGCTTAAGCGTCGCCGTGGCCGa                                                                                                                      >  2:36858/1‑139 (MQ=255)

                   aGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTATGTCGGCGATGATCTCATCGACTGGCCGGTAATGGAAAAAGTGGGCTTAAGCGTCGCCGTGGCCGATgc                                                                                                                   >  2:106422/1‑139 (MQ=255)

                     tCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTATGTCGGCGATGATCTCATCGACTGGCCGGTAATGGAAAAAGTGGGCTTAAGCGTCGCCGTGGCCGATgcgc                                                                                                                 <  1:106422/139‑1 (MQ=255)

                      cagacaaacTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTATGTCGGCGATGATCTCATCGACTGGCCGGTAATGGAAAAAGTGGGCTTAAGCGTCGCCGTGGCCGATGCGCa                                                                                                                <  2:446837/136‑1 (MQ=255)

                        aacaaacTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTATGTCGGCGATGATCTCATCGACTGGCCGGTAATGGAAAAAGTGGGCTTAAGCGTCGCCGTGGCCGATGCGCATc                                                                                                              <  2:1374996/139‑1 (MQ=255)

                         acaaacTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTATGTCGGCGATGATCTCATCGACTGGCCGGTAATGGAAAAAGTGGGCTTAAGCGTCGCCGTGGCCGATGCGCATcc                                                                                                             >  1:1024503/1‑139 (MQ=255)

                                 aTCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTATGTCGGCGATGATCTCATCGACTGGCCGGTAATGGAAAAAGTGGGCTTAAGCGTCGCCGTGGCCGATGCGCATCCACTGTTGa                                                                                                     >  2:1476089/1‑139 (MQ=255)

                                   cGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTATGTCGGCGATGATCTCATCGACTGGCCGGTAATGGAAAAAGTGGGCTTAAGCGTCGCCGTGGCCGATGCGCATCCACTGTTGATc                                                                                                   >  1:464130/1‑139 (MQ=255)

                                   cGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTATGTCGGCGATGATCTCATCGACTGGCCGGTAATGGAAAAAGTGGGCTTAAGCGTCGCCGTGGCCGATGCGCATCCACTGTTGATc                                                                                                   >  1:182708/1‑139 (MQ=255)

                                     ccTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTATGTCGGCGATGATCTCATCGACTGGCCGGTAATGGAAAAAGTGGGCTTAAGCGTCGCCGTGGCCGATGCGCATCCACTGTTGATccc                                                                                                 >  2:308502/1‑139 (MQ=255)

                                       tttAGCGCTCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTATGCCGGCGACGATCCCATCGACTGGCCGGTAATGGAAAAAGTGGGCTTAAGCGTCGCCGTGGCCGATGCGCATCCACTGTTGATCCcgc                                                                                               >  1:1453272/1‑139 (MQ=255)

                                        ttAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTATGTCGGCGATGATCTCATCGAATGGCCGGTAATGGAAAAAGTGGGCTTAAGCGTCGCCGTGGCCGATGCGCATCCACTGTTGATCCcgcg                                                                                              <  2:1024503/139‑1 (MQ=255)

                                           gCGATCTGCTGGAAAAACTGGCGATTGCCCGGGAAAATGTGGCTTATGTCGGCGATGATCTCATCGACTGGCCGGTAATGGAAAAAGTGGGCTTAAGCGTCGCCGTGGCCGATGCGCATCCACTGTTGATCCCgcgcgc                                                                                           <  1:36858/139‑1 (MQ=255)

                                           gCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTATGTCGGCGATGATCTCATCGACTGGCCGGTAATGGAAAAAGTGGGCTTAAGCGTCGCCGTGGCCAATGCGCATCCACTGTTGATCCCgcgcgc                                                                                           >  1:1368269/1‑139 (MQ=255)

                                                ctgctgGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTATGTCGGCGATGATCTCATCGACTGGCCGGTAATGGAAAAAGTGGGCTTAAGCGTCGCCGTGGCCGATGCGCATTCACTGTTg                                                                                                      <  2:1070652/123‑1 (MQ=255)

                                                ctgctgGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTATGTCGGCGATGATCTCATCGACTGGCCGGTAATGGAAAAAGTGGGCTTAAGCGTCGCCGTGGCCGATGCGCATCCACTGTTg                                                                                                      >  1:1070652/1‑123 (MQ=255)

                                                ctgctgGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTATGTCGGCGATGATCTCATCGACTGGCCGGTAATGGAAAAAGTGGGCTTAAGCGTCGCCGTGGCCGATGCGCATCCACTGTTGATCCCGCGCGCCGAtt                                                                                      >  2:17367/1‑139 (MQ=255)

                                                ctgctgGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTATGTCGGCGATGATCTCATCGACTGGCCGGTAATGGAAAAAGTGGGCTTAAGCGTCGCCGTGGCCGATGCGCATCCACTGTTGATCCCGCGCGCCGAtt                                                                                      >  2:1140937/1‑139 (MQ=255)

                                                ctgctgGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTATGTCGGCGATGATCTCATCGACTGGCCGGTAATGGAAAAAGTGGGCTTAAGCGTCGCCGTGGCCGATGCGCATCCACTGTTGATCCCGCGCGCCGAtt                                                                                      >  2:626254/1‑139 (MQ=255)

                                                    tgGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTATGTCGGCGATGATCTCATCGACTGGCCGGTAATGGAAAAAGTGGGCTTAAGCGTCGCCGTGGCCGATGCGCATCCACTGTTGATCCCGCGCGCCGATTACGt                                                                                  >  1:58389/1‑139 (MQ=255)

                                                             tGGCGATTGCCCCGGAAAATGTGGCTTATGTCGGCGATGATCTCATCGACTGGCCGGTAATGGAAAAAGTGGGTTTAAGCGTCGCCGTGGCCGATGCGCATCCATTGTTGATCCCGCGCGCCGATTACGTGACGCGCAt                                                                         >  1:688241/1‑139 (MQ=255)

                                                             tGGCGATTGCCCCGGAAAATGTGGCTTATGTCGGCGATGATCTCATCGACTGGCCGGTAATGGAAAAAGTGGGCTTAAGCGTCGCCGTGGCCGa                                                                                                                      >  2:1531629/1‑94 (MQ=255)

                                                             tGGCGATTGCCCCGGAAAATGTGGCTTATGTCGGCGATGATCTCATCGACTGGCCGGTAATGGAAAAAGTGGGCTTAAGCGTCGCCGTGGCCGa                                                                                                                      <  1:1531629/94‑1 (MQ=255)

                                                             tGGCGATTGCCCCGGAAAATGTGGCTTATGTCGGCGATGATCTCATCGACTGGCCGGTAATGGAAAAAGTGGGCTTAAGCGTCGCCGTGGCCGATGCGCAt                                                                                                               <  1:449133/101‑1 (MQ=255)

                                                             tGGCGATTGCCCCGGAAAATGTGGCTTATGTCGGCGATGATCTCATCGACTGGCCGGTAATGGAAAAAGTGGGCTTAAGCGTCGCCGTGGCCGATGCGCAt                                                                                                               >  2:449133/1‑101 (MQ=255)

                                                                    tGCCCCGGAAAATGTGGCTTATGTCGGCGATGATCTCATCGACTGGCCGGTAATGGAAAAAGTGGGCTTAAGCGTCGCCGTGGCCGATGCGCATCCACTGTTGATCCCGCGCGCCGATTACGTGACGCGCATTGCTGGc                                                                  <  1:557335/139‑1 (MQ=255)

                                                                        ccGGAAAATGTGGCTTATGTCGGCGATGATCTCATCGACTGGCCGGTAATGGAAAAAGTGGGTTTAAGCGTCGCCGTGGCCGATGCGCATCCATTGTTGATCCCGCGCGCCGATTACGTGACGCGCATTGCTGGCGGTc                                                              <  2:688241/139‑1 (MQ=255)

                                                                               aTGTGGCTTATGTCGGCGATGATCTCATCGACTGGCCGGTAATGGAAAAAGTGGGCTTAAGCGTCGCCGTGGCCGATGCGCATCCACTGTTGATCCCGCGCGCCGATTACGTGACGCGCATTGCTGGCGGTCGTGgcgc                                                       <  2:464130/139‑1 (MQ=255)

                                                                               aTGTGGCTTATGTCGGCGATGATCTCATCGACTGGCCGGTAATGGAAAAAGTGGGCTTAAGCGTCGCCGTGGCCGATGCGCATCCACTGTTGATACCGCGCGCCGATTACGTGACGCGCATTGCTGGCGGTCGTGgcgc                                                       <  1:1162833/139‑1 (MQ=255)

                                                                               aTGTGGCTTATGTCGGCGATGATCTCATCGACTGGCCGGTAATGGAAAAAGTGGGCTTAAGCGTCGCCGTGGCCAATGCGCATCCACTGTTGATCCCGCGCGCCGATTACGTGACGCGCATTGCTGGCGGTCGTGgcgc                                                       <  2:1368269/139‑1 (MQ=255)

                                                                               aTGTGGCTTATGTCGGCGATGATCTCATCGACTGGCCGGTAATGGAAAAAGTGGGCTTAAGCGTCGCCGAGGCCGATGCGCATCCACTGTTGATCCCGCGCGCCGATTACGTGACGCGCATTGCTGGCGGTCGTGtcgc                                                       <  1:1476089/139‑1 (MQ=255)

                                                                               aTGTGGCTTATGTCGGCGATGATCTCATCGACTGGCCGGTAATGGAAAAAGTGGGCTTAAGCGTCGCCGAGGCCGATGCGCATCCACTGTTGATCCCGCGCGCCGATTACGTGACGCGCATTGCTGGCGGTCGTGgcgc                                                       <  1:645625/139‑1 (MQ=255)

                                                                                        aTGTCGGCGATGATCTCATCGACTGGCCGGTAATGGAAAAAGTGGGCTTAAGCGTCGCCGTGGCCGa                                                                                                                      >  2:735840/1‑67 (MQ=255)

                                                                                        aTGTCGGCGATGATCTCATCGACTGGCCGGTAATGGAAAAAGTGGGCTTAAGCGTCGCCGTGGCCGa                                                                                                                      <  1:735840/67‑1 (MQ=255)

                                                                                          gTCGGCGATGATCTCATCGACTGGCCGGTAATGGAAAAAGTGGGCTTAAGCGTCGCCGTGGCCGATGCGCATCCACTGTTGATCCCGCGCGCCGATTACGTGACGCGCATTGCTGGCGGTCGTGGCGCAGTGCGCGAAg                                            >  2:1461265/1‑139 (MQ=255)

                                                                                              gCGATGATCTCATCGACTGGCCGGTAATGGAAAAAGTGGGTTTAAGCGTCGCCGTGGCCGATGCGCATCCACTGTTGATCCCGCGCGCCGATTACGTGACGCGCATTGCTGGCGGTCGTGGCGCAGTGCGCGAAGTTTg                                        <  1:1159212/139‑1 (MQ=255)

                                                                                              gCGATGATCTCATCGACTGGCCGGTAATGGAAAAAGTGGGCTTAAGCGTCGCCGTGGCCGATGCGCATCCACTGTTGATCCCGCGCGCCGATTACGTGACGCGCATTGCTGGCGGTCGTGGCGCAGTGCGCGAAGTTTg                                        >  2:1346247/1‑139 (MQ=255)

                                                                                                 atgatCTCATCGACTGGCCGGTAATGGAAAAAGTGGGCTTAAGCGTCGCCGTGGCCGATGCGCATCCACTGTTGATCCCGCGCGCCGATTACGTGACGCGCATTGCTGGCGGTCGTGGCGCAGTGCGCGAAGTTTGCGa                                     >  1:345305/1‑139 (MQ=255)

                                                                                                  tgatCTCATCGACTGGCCGGTAATGGAAAAAGTGGGCTTAAGCGTCGCCGTGGCCGATGCGCAt                                                                                                               >  2:1033899/1‑64 (MQ=255)

                                                                                                  tgatCTCATCGACTGGCCGGTAATGGAAAAAGTGGGCTTAAGCGTCGCCGTGGCCGATGCGCAt                                                                                                               <  1:1033899/64‑1 (MQ=255)

                                                                                                   gatCTCATCGACTGGCCGGTAATGG‑AAAAGTGGGCTTAAGCGTCGCCGTGGCCGATGCGCATCCACTGTTGGTCCCGCGCGCCGATTACGTGACGCGCATTGCTGGCGGTCGTGGCGCAGTGCGCGAAGTTTGCGACtt                                  >  1:946806/1‑139 (MQ=255)

                                                                                                              cTGGCCGGTAATGGAAAAAGTGGGCTTAAGCGTCGCCGTGGCCGATgcgc                                                                                                                 >  1:1332019/1‑50 (MQ=255)

                                                                                                              cTGGCCGGTAATGGAAAAAGTGGGCTTAAGCGTCGCCGTGGCCGATgcgc                                                                                                                 <  2:1332019/50‑1 (MQ=255)

                                                                                                              cTGGCCGGTAATGGAAAAAGTGGGCTTAAGCGTCGCCGTGGCCGATGCGCATCCACTGTTGATCCCGCGCGCCGATTACGTg                                                                                 >  1:1448721/1‑82 (MQ=255)

                                                                                                              cTGGCCGGTAATGGAAAAAGTGGGCTTAAGCGTCGCCGTGGCCGATGCGCATCCACTGTTGATCCCGCGCGCCGATTACGTg                                                                                 <  2:1448721/82‑1 (MQ=255)

                                                                                                              cTGGCCGGTAATGGAAAAAGTGGGCTTAAGCGTCGCCGTGGCCGATGCGCATCCACTGTTGATCCCGCGCGCCGATTACGTGACGCGCATTGCTGGCGGTCGTGGCGCAGTGCGCGAAGTTTGCGACTTATTACTCCTg                        >  2:1177228/1‑139 (MQ=255)

                                                                                                               tGGCCGGTAATGGAAAAAGTGGGTTTAAGCGTCGCCg                                                                                                                             >  2:931417/1‑37 (MQ=255)

                                                                                                               tGGCCGGTAATGGAAAAAGTGGGTTTAAGCGTCGCCg                                                                                                                             <  1:931417/37‑1 (MQ=255)

                                                                                                                 gCCGGTAATGGAAAAAGTGGGTTTAAGCGTCGCCGTGGCCGATGCGCATCCACTGTTGATCCCGCGCGCCGATTACGTGACGCGCATTGCTGGCgg                                                                <  2:343002/96‑1 (MQ=255)

                                                                                                                 gCCGGTAATGGAAAAAGTGGGTTTAAGCGTCGCCGTGGCCGATGCGCATCCACTGTTGATCCCGCGCGCCGATTACGTGACGCGCATTGCTGGCgg                                                                >  1:343002/1‑96 (MQ=255)

                                                                                                                 gCCGGTAATGGAAAAAGTGGGCTTAAGCGTCGCCGTGGCCGATGCGCATcc                                                                                                             <  2:1381568/51‑1 (MQ=255)

                                                                                                                 gCCGGTAATGGAAAAAGTGGGCTTAAGCGTCGCCGTGGCCGATGCGCATcc                                                                                                             >  1:1381568/1‑51 (MQ=255)

                                                                                                                 gCCGGTAATGGAAAAAGTGGGCTTAAGCGTCGCCGTGGCCGATGCGCATCCACTGTTGATCCCGCGCGCCGATTACGTGACGCGCATTGCTGGCGGTCGTGGCGCAGTGCGCGAAGTTTGCGACTTATTACTCCTGgcg                     <  2:345305/139‑1 (MQ=255)

                                                                                                                 gCCGGTAATGGAAAAAGTGGGCTTAAGCGTCGCCGTGGCCGATGCGCATCCACTGTTGATCCCGCGCGCCGATTACGTGACGCGCATTGCTGGCGGTCGTGGCGCAGTGCGCGAAGTTTGCGACTTATTACTCCTGgcg                     >  1:268763/1‑139 (MQ=255)

                                                                                                                 gCCGGTAATGGAAAAAGTGGGCTTAAGCGTCGCCGTGGCCGATGCGCATCCACTGTTGATCCCGCGCGCCGATTACGTGACGCGCATTGCTGGCGGTCGTGGCGCAGTGCGCGAAGTTTGCGACTTATTACTCCTGgcg                     <  2:1453272/139‑1 (MQ=255)

                                                                                                                 gCCGGTAATGGAAAAAGTGGGCTTAAGCGTCGCCGTGGCCGATGCGCATCCACTGTTGATCCCGCGCGCCGATTACGTGACGCGCATTGCTGGCGGTCGTGGCGCAGTGCGCGAAGTTTGCGACTTATTACTCCTGgcg                     <  1:67561/139‑1 (MQ=255)

                                                                                                                      tAATGGAAAAAGTGGGCTTAAGCGTCGCCGTGGCCGATGCGCATCCACTGTTGATCCCGCGCGCCGATTACGTGACGCGCATTGCTGGCGGTCGTGGCGCAGTGCGCGAAGTTTGCGACTTATTACTCCTGGCGCAggg                <  2:817568/139‑1 (MQ=255)

                                                                                                                           gAAAAAGTGGGCTTAAGCGTCGCCGTGGCCGATGCGCATCCACTGTTGATCCCGCGCGCCGATTACGTGACGCGCATTGCTGGCGGTCGTGGCGCAGTGCGCGAAGTTTGCGACTTATTACTCCTGGCGCAGGGCAAAc           <  1:308502/139‑1 (MQ=255)

                                                                                                                            aaaaaGTGGGTTTAAGCGTCGCCGTGGCCGATGCGCATCCACTGTTGATCCCGCGCGCCGATTACGTGACGCGCATTGCTGGCGGTCGTGGCGCAGTGCGCGAAGTTTGCGa                                     >  1:1024148/1‑112 (MQ=255)

                                                                                                                            aaaaaGTGGGTTTAAGCGTCGCCGTGGCCGATGCGCATCCACTCTTGATCCCGCGCGCCGATTACGTGACGCGCATTGCTGGCGGTCGTGGCGCAGTGCGCGAAGTTTGCGa                                     <  2:1024148/112‑1 (MQ=255)

                                                                                                                               aaGTGGGCTTAAGCGTCGCCGTGGCCGATGCGCATCCACTGTTGATCCCGCGCGCCGATTACGTGACGCGCATTGCTGGCGGTCGTGGCGCAGTGCGCGAAGTTTGCGACTTATTACTCCTGGCGCAGGGCAAACTGGa       <  2:946806/139‑1 (MQ=255)

                                                                                                                                aGTGGGTTTAAGCGTCGCCGTGGCCGATGCGCATCCACTGTTGATCCCGCGCGCCGATTACGTGACGCGCATTGCTGGCGGTCGTGGCGCAGTGCGCGAAGttt                                         >  1:365151/1‑104 (MQ=255)

                                                                                                                                aGTGGGTTTAAGCGTCGCCGTGGCCGATGCGCATCCACTGTTGATCCCGCGCGCCGATTACGTGACGCGCATTGCTGGCGGTCGTGGCGCAGTGCGCGAAGttt                                         <  2:365151/104‑1 (MQ=255)

                                                                                                                                aGTGGGCTTAAGCGTCGCCGTGGCCGATGCGCATCCACTGTTGATCCCGCGCGCCGATTACGTGACGCGCATTGCTGGCGGTCGTGGCGCAGTGCGCGAAGTTTGCGa                                     >  1:847329/1‑108 (MQ=255)

                                                                                                                                aGTGGGCTTAAGCGTCGCCGTGGCCGATGCGCATCCACTGTTGATCCCGCGCGCCGATTACGTGACGCGCATTGCTGGCGGTCGTGGCGCAGTGCGCGAAGTTTGCGa                                     <  2:847329/108‑1 (MQ=255)

                                                                                                                                    ggTTTAAGCGTCGCCGTGGCCGATGCGCATCCACTGTTGATCCCGCGCGCCGATTACGTGACGCGCATTGCTGGCGGTCGTGGCGCAGTGCGCGAAGTTTGCGACTTATTACTCCTGGCGCAGGGCAAACTGGATGAAg  >  1:985407/1‑139 (MQ=255)

ACTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTATGTCGGCGATGATCTCATCGACTGGCCGGTAATGGAAAAAGTGGGTTTAAGCGTCGCCGTGGCCGATGCGCATCCACTGTTGATCCCGCGCGCCGATTACGTGACGCGCATTGCTGGCGGTCGTGGCGCAGTGCGCGAAGTTTGCGACTTATTACTCCTGGCGCAGGGCAAACTGGATGAAG  >  NC_000913/3342549‑3342819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A