Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F1 I2 R1	97	53.3	2484976	94.5%	2348302	111.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,765,794	(C)_7→8	intergenic (‑18/‑124)	yfjM ← / → rnlA	CP4‑57 prophage, uncharacterized protein/CP4‑57 prophage, RNase LS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,765,787	1	.	C	90.5%	202.8 / 14.0	63	intergenic (‑11/‑131)	yfjM/rnlA	CP4‑57 prophage, uncharacterized protein/CP4‑57 prophage, RNase LS
Reads supporting (aligned to +/- strand): ref base . (2/4); new base C (26/31); total (28/35)
Fisher's exact test for biased strand distribution p-value = 6.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.18e-01

CTCTGCTCGTTCTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGA  >  NC_000913/2765654‑2765916

ctctGCTCGTTCTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑‑ccccc                                                                                                                              <  2:28511/139‑1 (MQ=255)

 tctGCTCGTTCTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑‑cccccc                                                                                                                             >  1:924290/1‑139 (MQ=255)

       cGTTCTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑‑ccccccccggta                                                                                                                       <  2:322665/139‑6 (MQ=255)

        gTTCTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑‑CCCCCCCGGTAGa                                                                                                                      <  2:1175543/139‑1 (MQ=255)

           cTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑‑cccccccccggtagac                                                                                                                   >  2:1057552/1‑130 (MQ=255)

              tttCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATc                                                                                                                 <  2:736151/139‑1 (MQ=255)

                tCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGGATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCt                                                                                                               <  2:592627/139‑1 (MQ=255)

                  gACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑‑CCCCCCCGGTAGACGATCCTGcc                                                                                                            >  1:422672/1‑139 (MQ=255)

                    cGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGccc                                                                                                           >  2:92396/1‑139 (MQ=255)

                      tAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑‑cccc                                                                                                                               >  2:114553/1‑116 (MQ=255)

                      tAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑‑cccc                                                                                                                               <  1:114553/116‑1 (MQ=255)

                                tCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAg                                                                                                >  2:734255/1‑139 (MQ=255)

                                tCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGt                                                                                               <  1:92396/139‑1 (MQ=255)

                                 cTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGt                                                                                               <  1:734255/139‑1 (MQ=255)

                                            gCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTCAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATGTAATGGACTGC‑CCCCCCCGGTAGCCGATCCTGCCATATAGTTGGAGCGCCGCCTATTCa                                                                                   >  2:159119/1‑139 (MQ=255)

                                                          aGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTa                                                                     >  1:769867/1‑139 (MQ=255)

                                                          aGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAg                                                                    >  2:324914/1‑139 (MQ=255)

                                                            tATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAg                                                                    <  1:1057552/139‑1 (MQ=255)

                                                            tATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGc                                                                   >  2:115762/1‑139 (MQ=255)

                                                             aTCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCa                                                                  <  1:115762/139‑1 (MQ=255)

                                                               cGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGa                                                                                                 <  1:910019/106‑1 (MQ=255)

                                                               cGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGa                                                                                                 >  2:910019/1‑106 (MQ=255)

                                                               cGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑‑cc                                                                                                                                 >  1:1175735/1‑73 (MQ=255)

                                                               cGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑‑cc                                                                                                                                 <  2:1175735/73‑1 (MQ=255)

                                                               cGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCAT‑CCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGcc                                                                                            >  2:1004563/1‑110 (MQ=255)

                                                               cGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCAT‑CCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGcc                                                                                            <  1:1004563/110‑1 (MQ=255)

                                                                 aGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATc                                                              >  1:747073/1‑139 (MQ=255)

                                                                 aGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATc                                                              >  1:180493/1‑139 (MQ=255)

                                                                  gTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCaa                                                            <  1:324914/139‑1 (MQ=255)

                                                                    tgctgcTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAAt                                                           <  1:1220841/139‑1 (MQ=255)

                                                                    tgctgcTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATa                                                          <  2:422672/139‑1 (MQ=255)

                                                                     gctgctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑‑ccccccc                                                                                                                            <  1:474569/72‑1 (MQ=255)

                                                                     gctgctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑‑ccccccc                                                                                                                            >  2:474569/1‑72 (MQ=255)

                                                                      ctgctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTg                                                                                                              >  1:266268/1‑86 (MQ=255)

                                                                      ctgctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTg                                                                                                              <  2:266268/86‑1 (MQ=255)

                                                                      ctgctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCa                                                                  >  1:978449/1‑130 (MQ=255)

                                                                      ctgctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCa                                                                  <  2:978449/130‑1 (MQ=255)

                                                                      ctgctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATac                                                         >  1:1027279/1‑139 (MQ=255)

                                                                         ctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACt                                                      >  2:1106291/1‑139 (MQ=255)

                                                                         ctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑‑cccccccc                                                                                                                           >  1:1137556/1‑68 (MQ=255)

                                                                         ctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑‑cccccccc                                                                                                                           <  2:1137556/69‑2 (MQ=255)

                                                                              tGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAggg                                                <  1:537246/139‑1 (MQ=255)

                                                                               gagaTCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAggg                                                <  1:90520/139‑1 (MQ=255)

                                                                               gagaTCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAggg                                                <  1:159119/139‑1 (MQ=255)

                                                                               gagaTCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAggg                                                <  2:1104132/139‑1 (MQ=255)

                                                                                agaTCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAgggg                                               <  2:924290/139‑1 (MQ=255)

                                                                                 gaTCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATa                                                          >  2:1050825/1‑127 (MQ=255)

                                                                                 gaTCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATa                                                          <  1:1050825/127‑1 (MQ=255)

                                                                                  aTCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAgggggg                                             <  2:769867/139‑1 (MQ=255)

                                                                                    cAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGta                                           <  2:747073/139‑1 (MQ=255)

                                                                                     aGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGtat                                          <  1:1106291/139‑1 (MQ=255)

                                                                                        aaGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGtattat                                       <  1:383126/139‑1 (MQ=255)

                                                                                            ggTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCa                                                                  >  1:592302/1‑109 (MQ=255)

                                                                                            ggTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCa                                                                  <  2:592302/109‑1 (MQ=255)

                                                                                            ggTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGccc                                                                                                           <  1:1002517/67‑1 (MQ=255)

                                                                                            ggTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGccc                                                                                                           >  2:1002517/1‑67 (MQ=255)

                                                                                            ggTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑‑cccc                                                                                                                               <  2:151453/46‑1 (MQ=255)

                                                                                            ggTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑‑cccc                                                                                                                               >  1:151453/1‑46 (MQ=255)

                                                                                             gTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTag                                  >  2:1052830/1‑139 (MQ=255)

                                                                                               tACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGTAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTagag                                >  1:119341/1‑139 (MQ=255)

                                                                                                aCCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATc                                                                                                                 <  1:876837/57‑1 (MQ=255)

                                                                                                aCCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATc                                                                                                                 >  2:876837/1‑57 (MQ=255)

                                                                                                aCCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGta                                           <  1:1103356/127‑1 (MQ=255)

                                                                                                aCCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGta                                           >  2:1103356/1‑127 (MQ=255)

                                                                                                aCCGAAACGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGt                               <  2:1027279/139‑1 (MQ=255)

                                                                                                     aaCGATGGACATCATCCCCCATTTTGTGGACTG‑‑ccccc                                                                                                                              >  2:663867/1‑38 (MQ=255)

                                                                                                     aaCGATGGACATCATCCCCCATTTTGTGGACTG‑‑ccccc                                                                                                                              <  1:663867/38‑1 (MQ=255)

                                                                                                      aCGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCa                                                                                   >  1:1113450/1‑81 (MQ=255)

                                                                                                      aCGATGGACATCATCCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCa                                                                                   <  2:1113450/81‑1 (MQ=255)

                                                                                                                 catcCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAg                                                  >  2:1023277/1‑103 (MQ=255)

                                                                                                                 catcCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAg                                                  <  1:1023277/103‑1 (MQ=255)

                                                                                                                  atcCCCCTTTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACCCTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTAt             <  1:192565/139‑1 (MQ=255)

                                                                                                                  atcCCCCATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTAt             <  2:180493/139‑1 (MQ=255)

                                                                                                                        cATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCa                                                                  >  1:322888/1‑80 (MQ=255)

                                                                                                                        cATTTTGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCa                                                                  <  2:322888/80‑1 (MQ=255)

                                                                                                                           tttGTGGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAg    >  1:589779/1‑139 (MQ=255)

                                                                                                                             tgtgGACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGa  >  2:533804/1‑139 (MQ=255)

                                                                                                                                ggACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGcccc                                                                                          <  2:835762/48‑1 (MQ=255)

                                                                                                                                ggACTGC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGcccc                                                                                          >  1:835762/1‑48 (MQ=255)

CTCTGCTCGTTCTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGA  >  NC_000913/2765654‑2765916

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A