Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F1 I2 R1
|
97 |
53.3 |
2484976 |
94.5% |
2348302 |
111.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,791,004 |
A→G |
G114G (GGT→GGC) |
tdh ← |
L‑threonine 3‑dehydrogenase, NAD(P)‑binding |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,791,004 | 0 | A | G | 100.0%
| 156.6
/ NA
| 42 | G114G (GGT→GGC) | tdh | L‑threonine 3‑dehydrogenase, NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (20/22); total (20/22) |
GACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCAATACCTACC > NC_000913/3790870‑3791139
|
gACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCaa < 2:1099305/139‑1 (MQ=255)
aGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGc > 2:294944/1‑139 (MQ=255)
gcgGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTa < 1:823656/139‑1 (MQ=255)
cGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGca < 1:748086/139‑1 (MQ=255)
tACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACaa > 1:468066/1‑139 (MQ=255)
aCCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGTGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACaaa < 1:682218/139‑1 (MQ=255)
gggATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTa > 1:807027/1‑139 (MQ=255)
aTCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGt < 1:930466/135‑1 (MQ=255)
aTCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGt > 2:930466/1‑135 (MQ=255)
gCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGAc > 1:100213/1‑139 (MQ=255)
cGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATg > 1:567229/1‑139 (MQ=255)
atatTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACcac > 1:549105/1‑139 (MQ=255)
atatTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACcac < 2:100213/139‑1 (MQ=255)
ggggATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACaaa > 1:928253/1‑94 (MQ=255)
ggggATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACaaa < 2:928253/94‑1 (MQ=255)
ggggATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGata > 2:1038713/1‑139 (MQ=255)
ggggATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGata > 2:277336/1‑139 (MQ=255)
ttttGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGAc > 1:874477/1‑123 (MQ=255)
ttttGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGcc < 2:567229/139‑1 (MQ=255)
ttttGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGcc < 1:639970/139‑1 (MQ=255)
ttttGAAGGCGTTGAATGCCGGGAACACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGAc < 2:874477/123‑1 (MQ=255)
ttGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCa > 2:355642/1‑107 (MQ=255)
ttGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCa < 1:355642/107‑1 (MQ=255)
cGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGcc > 1:36243/1‑120 (MQ=255)
cGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGcc < 2:36243/120‑1 (MQ=255)
aCCTGATATTCGGCAAAGCAGCCCGGGCGATTAACGcc > 2:1240242/1‑38 (MQ=255)
aCCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGcc < 1:1240242/38‑1 (MQ=255)
ccAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACaaa < 1:130737/62‑1 (MQ=255)
ccAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACaaa > 2:130737/1‑62 (MQ=255)
atatTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAg < 1:1117343/139‑1 (MQ=255)
tatTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGc > 1:646280/1‑139 (MQ=255)
tatTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGc < 2:468066/139‑1 (MQ=255)
atTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACg < 1:865044/64‑1 (MQ=255)
atTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACg > 2:865044/1‑64 (MQ=255)
atTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGcc > 1:196759/1‑139 (MQ=255)
tCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGacc > 1:918359/1‑65 (MQ=255)
tCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGacc < 2:918359/65‑1 (MQ=255)
gCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCa > 1:931260/1‑139 (MQ=255)
agcagcCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTc < 1:294944/139‑1 (MQ=255)
cccGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGAc < 1:437749/139‑1 (MQ=255)
gggCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCaa < 2:807027/139‑1 (MQ=255)
gATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCAAtacc < 1:277336/139‑1 (MQ=255)
aacgccaacgccTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCAAtacctacc < 2:644420/139‑1 (MQ=255)
|
GACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCAATACCTACC > NC_000913/3790870‑3791139
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
