Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F1 I2 R1
|
97 |
53.3 |
2484976 |
94.5% |
2348302 |
111.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,347,002 |
C→T |
G109S (GGC→AGC) |
fumB ← |
anaerobic class I fumarate hydratase (fumarase B) |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,347,002 | 0 | C | T | 100.0%
| 84.3
/ NA
| 26 | G109S (GGC→AGC) | fumB | anaerobic class I fumarate hydratase (fumarase B) |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (13/13); total (13/13) |
GCGCCGCATTCTGTGAATAGCGCAGGTTATCTTCGATATAGGTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCTTTTTTACCGACGATGATCGCGGTGCCGGTATCCTGGCAGGTCGGCAGCACGCCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTCGTTTTCGCTGGCTTCTGGATCGTGAAGAATAGCCGCAACCTGTTTCTGGT > NC_000913/4346873‑4347130
|
gcgcCGCATTCTGTGAATAGCGCAGGTTATCTTCGATATAGGTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCTTTTTTACCGACGATGATCGCGGTGCTGGTATCCTg > 2:998507/1‑139 (MQ=255)
cgccgcATTCTGTGAATAGCGCAGGTTATCTTCGATATAGGTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCTTTTTTACCGACGATGATCGCGGTGCTGGTATCCTgg < 2:9389/139‑1 (MQ=255)
gcgcAGGTTATCTTCGATATAGGTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCTTTTTTACCGACGATGATCGCGGTGCTGGTATCCTGGCAGGTCGGCAGCACGCCt > 2:95186/1‑139 (MQ=255)
ggtATCTTCGATATAGGTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCTTTTTTACCGACGATGATCACGGTGCTGGTATCCTGGCAGGTCGGCAGCACGCCTTTggcg > 2:742237/3‑139 (MQ=255)
ttATCTTCGATATAGGTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCTTTTTTACCGACGATGATCGCGGTGCTGGTATCCTGGCAGGTCGGCAGCACGCCTTTggcgg < 2:902822/139‑1 (MQ=255)
aGGTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCTTTTTTACCGACGATGATCGCGGTGCTGGTATCCTGGCAGGTCGgcag < 2:97813/112‑1 (MQ=255)
aGGTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCTTTTTTACCGACGATGATCGCGGTGCTGGTATCCTGGCAGGTCGgcag > 1:97813/1‑112 (MQ=255)
ataGACGCCATTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCTTTTTTACCGACGATGATCGCGGTGCTGGTATCCTGGCAGGTCGGCAGCACGCCTTTGGCGGCGATTTCGGAGTTTCTTAAg > 2:989770/1‑139 (MQ=255)
gACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCTTTTTTACCGACGATGATCGCGGTGCTGGTATCCTGGCAGGTCGGCAGCACGCCTTTGGCGGCGATTTCGGAGTTTCTTAAGAAt > 1:1154719/1‑139 (MQ=255)
cGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCTTTTTTACCGACGATGATCGCGGTGCTGGTATCCTGGCAGGTCGGCAGCACGCCTTTGGCGGCGATTTCGGAGt > 1:409720/1‑126 (MQ=255)
cGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCTTTTTTACCGACGATGATCGCGGTGCTGGTATCCTGGCAGGTCGGCAGCACGCCTTTGGCGGCGATTTCGGAGt < 2:409720/126‑1 (MQ=255)
cTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCTTTTTTACCGACGATGATCGCGGTGCTGGTATCCTGGCAGGTCGGCAGCACGCCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAg > 1:1171898/1‑139 (MQ=255)
cTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCTTTTTTACCGACGATGATCGCGGTGCTGGTATCCTGGCAGGTCGGCAGCACGCCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAg > 2:503687/1‑139 (MQ=255)
cGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCTTTTTTACCGACGATGATCGCGGTGCTGGTATCCTGGCAGGTCGGCAGCACGCCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTAc > 1:294231/1‑139 (MQ=255)
cgccgccgGTCCACACGCGCTGGCCTTTTTTACCGACGATGATCGCGGTGCTGGTATCCTGGCAGGTCGGCAGCACGCCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTCGTTTTCGCt < 2:309886/139‑1 (MQ=255)
cgccgccgGTCCACACGCGCTGGCCTTTTTTACCGACGATGATCGCGGTGCTGGTATCCTGGCAGGTCGGCAGCACGCCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTCGTTTTCGCt < 2:1171898/139‑1 (MQ=255)
cgccgccgGTCCACACGCGCTGGCCTTTTTTACCGACGATGATCGCGGTGCTGGTATCCTGGCAGGTCGGCAGCACGCCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTCGTTTTCGCt < 1:503687/139‑1 (MQ=255)
cgccgccgGTCCACACGCGCTGGCCTTTATTACCGACGATGATCGCGGTGCTGGTATCCTGGCAGGTCGGCAGCACGCCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTCGTTTTCGCt < 1:742237/139‑1 (MQ=255)
ccgccgGTCCACACGCGCTGGCCTTTTTTACCGACGATGATCGCGGTGCTGGTATCCTGGCAGGTCGGCAGCACGCCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTCGTTTTCGCTgg < 2:294231/139‑1 (MQ=255)
ccgGTCCACACGCGCTGGCCTTTTTTACCGACGATGATCGCGGTGCTGGTATCCTGGCAGGTCGGCAGCACGCCTTTGGCAGCGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTCGTTTTCGCTGGCtt < 2:458407/139‑1 (MQ=255)
gcTGGCCTTTTTTACCGACGATGATCGCGGTGCTGGTATCCTGGCAGGTCGGCAGCACGCCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTCGTTTTCGCTGGCTTCTGGATCGTgaag > 2:194902/1‑139 (MQ=255)
cTGGCCTTTTTTACCGACGATGATCGCGGTGCTGGTATCCTGGCAGGTCGGCAGCACGCCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTCGTTTTCGCTGGCTTCTGGATCGTgaaga > 1:586515/1‑139 (MQ=255)
ttACCGACGATGATCGCGGTGCTGGTATCCTGGCAGGTCGGCAGCACGCCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTCGTTTTCGCTGGCTTCTGGATCGTGAAGAATAGCCGCaa < 2:52796/139‑1 (MQ=255)
gacgaTGATCGCGGTGCTGGTATCCTGGCAGGTCGGCAGCACGCCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTCGTTTTCGCTGGCTTCTGGATCGTGAAGAATAGCCGCAACCTGt < 1:989770/139‑1 (MQ=255)
gatgatCGCGGTGCTGGTATCCTGGCAGGTCGGCAGCACGCCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTCGTTTTCGCTGGCTTCTGGATCGTGAAGAATAGCCGCAACCTGTTTc > 2:724503/1‑139 (MQ=255)
atCGCGGTGCTGGTATCCTGGCAGGTCGGCAGCACGCCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTCGTTTTCGCTGGCTTCTGGATCGTGAAGAATAGCCGCAACCTGTTTCTGgt < 1:95186/139‑1 (MQ=255)
|
GCGCCGCATTCTGTGAATAGCGCAGGTTATCTTCGATATAGGTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCTTTTTTACCGACGATGATCGCGGTGCCGGTATCCTGGCAGGTCGGCAGCACGCCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTCGTTTTCGCTGGCTTCTGGATCGTGAAGAATAGCCGCAACCTGTTTCTGGT > NC_000913/4346873‑4347130
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
