Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F1 I2 R1	97	53.3	2484976	94.5%	2348302	111.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,636,673	G→A	G223R (GGA→AGA)	creB →	response regulator in two‑component regulatory system with CreC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,636,673	0	G	A	100.0%	159.9 / NA	47	G223R (GGA→AGA)	creB	response regulator in two‑component regulatory system with CreC
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (24/23); total (24/23)

CAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAG  >  NC_000913/4636538‑4636802

cAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGAGat                                                                                                                                >  2:496300/1‑139 (MQ=255)

     gATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGAGATATAGc                                                                                                                           <  1:676865/139‑1 (MQ=255)

         gATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGAGATATAGCCTGa                                                                                                                       <  1:496300/139‑1 (MQ=255)

              cGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGAGATATAGCCTGAGGGGc                                                                                                                  >  2:1183512/1‑139 (MQ=255)

                     gAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGAGATATAGCCTGAGGGGCCTGTAAt                                                                                                           <  1:158329/139‑1 (MQ=255)

                        gATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGAGATATAGCCTGAGGGGCCTGTAATGCg                                                                                                        >  1:811371/1‑139 (MQ=255)

                           gcgcAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGAGATATAGCCTGAGGGGCCTGTAATGCGTAt                                                                                                     <  2:338401/139‑1 (MQ=255)

                                           aTCGCACCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGAGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGttgttg                                                                                     <  2:982319/139‑1 (MQ=255)

                                             cGCACCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGAGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCt                                                                                   >  1:286633/1‑139 (MQ=255)

                                               cACCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGAGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTgg                                                                                 >  1:844972/1‑139 (MQ=255)

                                               cACCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGAGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTgg                                                                                 >  1:740193/1‑139 (MQ=255)

                                                aCCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGAGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTggg                                                                                >  1:1122594/1‑139 (MQ=255)

                                                aCCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGAGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTggg                                                                                <  1:246409/139‑1 (MQ=255)

                                                  cGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGAGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCt                                                                              <  2:227206/139‑1 (MQ=255)

                                                    tCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGAGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTAt                                                                            >  2:1020994/1‑139 (MQ=255)

                                                        tACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCCTGAGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGATAttttt                                                                        <  2:811371/139‑1 (MQ=255)

                                                              caTTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGAGATATAGCCTGAGGGGc                                                                                                                  >  2:274378/1‑91 (MQ=255)

                                                              caTTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGAGATATAGCCTGAGGGGc                                                                                                                  <  1:274378/91‑1 (MQ=255)

                                                                         tGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGAGATATAGCCTGAGGGGc                                                                                                                  <  1:1179803/80‑1 (MQ=255)

                                                                         tGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGAGATATAGCCTGAGGGGc                                                                                                                  >  2:1179803/1‑80 (MQ=255)

                                                                           cGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGAGAtata                                                                                                                             >  2:446670/1‑67 (MQ=255)

                                                                           cGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGAGAtata                                                                                                                             <  1:446670/67‑1 (MQ=255)

                                                                           cGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGAGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGc                                                     <  1:1020994/139‑1 (MQ=255)

                                                                           cGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGAGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGc                                                     <  2:1185799/139‑1 (MQ=255)

                                                                           cGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGAGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGc                                                     >  1:177627/1‑139 (MQ=255)

                                                                             tGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGAGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCt                                                   >  1:190676/1‑139 (MQ=255)

                                                                                cAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGAGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGt                                                <  2:1128635/139‑1 (MQ=255)

                                                                                cAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGAGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGt                                                <  1:382225/139‑1 (MQ=255)

                                                                                     tGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGAGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTa                                           <  2:190676/139‑1 (MQ=255)

                                                                                                      gATCTTTCACCGATTAATACTCATCGCGGCATGAGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGt                                                >  1:869623/1‑117 (MQ=255)

                                                                                                      gATCTTTCACCGATTAATACTCATCGCGGCATGAGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGt                                                <  2:869623/117‑1 (MQ=255)

                                                                                                             cACCGATTAATACTCATCGCGGCATGAGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGttgttg                                                                                     <  2:722814/73‑1 (MQ=255)

                                                                                                             cACCGATTAATACTCATCGCGGCATGAGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGttgttg                                                                                     >  1:722814/1‑73 (MQ=255)

                                                                                                             cACCGATTAATACTCATCGCGGCATGAGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGtt                   >  2:772049/1‑139 (MQ=255)

                                                                                                                  aTTAATACTCATCGCGGCATGAGATATAGCCTGAggg                                                                                                                    <  1:742803/37‑1 (MQ=255)

                                                                                                                  aTTAATACTCATCGCGGCATGAGATATAGCCTGAggg                                                                                                                    >  2:742803/1‑37 (MQ=255)

                                                                                                                  aTTAATACTCATCGCGGCATGAGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGc                                                     <  2:248266/100‑1 (MQ=255)

                                                                                                                  aTTAATACTCATCGCGGCATGAGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGc                                                     <  2:30759/100‑1 (MQ=255)

                                                                                                                  aTTAATACTCATCGCGGCATGAGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGc                                                     >  1:248266/1‑100 (MQ=255)

                                                                                                                  aTTAATACTCATCGCGGCATGAGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGc                                                     >  1:30759/1‑100 (MQ=255)

                                                                                                                  aTTAATACTCATCGCGGCATGAGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAAcc              >  2:362797/1‑139 (MQ=255)

                                                                                                                  aTTAATACTCATCGCGGCATGAGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAAcc              >  2:938885/1‑139 (MQ=255)

                                                                                                                           cATCGCGGCATGAGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCg     <  1:362797/139‑1 (MQ=255)

                                                                                                                            aTCGCGGCATGAGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGa    >  2:654920/1‑139 (MQ=255)

                                                                                                                              cgcgGCATGAGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTggg                                                                                <  2:1066109/61‑1 (MQ=255)

                                                                                                                              cgcgGCATGAGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTggg                                                                                >  1:1066109/1‑61 (MQ=255)

                                                                                                                              cgcgGCATGAGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAag  >  2:26859/1‑139 (MQ=255)

CAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAG  >  NC_000913/4636538‑4636802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A