Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F1 I2 R1	77	34.8	1608548	95.0%	1528120	114.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,889,269	C→T	L277L (CTG→CTA)	cysI ←	sulfite reductase, beta subunit, NAD(P)‑binding, heme‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,889,269	0	C	T	100.0%	75.3 / NA	23	L277L (CTG→CTA)	cysI	sulfite reductase, beta subunit, NAD(P)‑binding, heme‑binding
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (11/12); total (11/12)

CCGCTTTAAACGTCTCAACCCCCACGCGCTCCAGCGTGTATTTGGTTTTGGCATTTTTACGATCGGTTCGGTTACCCCAGTCACGCTGAGTTGTCACGACGGCTTCGGCCACCGCCAGCGTATGCTCCAGCGGCAGATAGCCAAACTCACTCGCGGTGCGGGCGTAGGTTTTCTTGTTGCCGTGTTCGATGGAAAGCCCACCGCCCACCAACAGGTTAAAGCCCACCAGCTTGCCGTTTTCGGCGATCGCCACGAAGTTCA  >  NC_000913/2889136‑2889396

ccGCTTTAAACGTCTCAACCCCCACGCGCTCCAGCGTGTATTTGGTTTTGGCATTTTTACGATCGGTTCGGTTACCCCAGTCACGCTGAGTTGTCACGACGGCTTCGGCCACCGCCAGCGTATGCTCCAGCGGTAGATa                                                                                                                            >  1:621682/1‑139 (MQ=255)

  gCTTTAAACGTCTCAACCCCCACGCGCTCCAGCGTGTATTTGGTTTTGGCATTTTTACGATCGGTTCGGTTACCCCAGTCACGCTGAGTTGTCACGACGGCTTCGGCCACCGCCAGCGTATGCTCCAGCGGTAGATAGc                                                                                                                          >  2:253015/1‑139 (MQ=255)

              tcAACCCCCACGCGCTCCAGCGTGTATTTGGTTTTGGCATTTTTACGATCGGTTCGGTTACCCCAGTCACGCTGAGTTGTCACGACGGCTTCGGCCACCGCCAGCGTATGCTCCAGCGGTAGATAGCCAAACTCACTcg                                                                                                              <  2:621682/139‑1 (MQ=255)

                            cTCCAGCGTGTATTTGGTTTTGGCATTTTTACGATCGGTTCGGTTACCCCAGTCACGCTGAGTTGTCACGACGGCTTCGGCCACCGCCAGCGTATGCTCCAGCGGTAGATAACCAAACTCACTCGCGGTGCGGGCGTAg                                                                                                >  2:671118/1‑139 (MQ=255)

                                aGCGTGTATTTGGTTTTGGCATTTTTACGATCGGTTCGGTTACCCCAGTCACGCTGAGTTGTCACGACGGCTTCGGCCACCGCCAGCGTATGCTCCAGCGGTAGATAGCCAAACTCACTCGCGGTGCGGGCGTAGGttt                                                                                            <  2:632086/139‑1 (MQ=255)

                                                                 gttcggttACCCCAGTCACGCTGAGTTGTCACGACGGCTTCGGCCACCGCCAGCGTATGCTCCAGCGGTAGATAGCCAAACTCACTcg                                                                                                              <  1:307603/88‑1 (MQ=255)

                                                                 gttcggttACCCCAGTCACGCTGAGTTGTCACGACGGCTTCGGCCACCGCCAGCGTATGCTCCAGCGGTAGATAGCCAAACTCACTcg                                                                                                              >  2:307603/1‑88 (MQ=255)

                                                                        tACCCCAGTCACGCTGAGTTGTCACGACGGCTTCGGCCACCGCCAGCGTATGCTCCAGCGGTAGATAGCCAAACTCACTCGCGGTGCGGGCGTAGGTTTTCTTGTTGCCGTGTTCGATGGAAAGCCCACCGCCCACCaa                                                    <  1:253015/139‑1 (MQ=255)

                                                                            ccAGTCACGCTGAGTTGTCACGACGGCTTCGGCCACCGCCAGCGTATGCTCCAGCGGTAGATAGCCAAACTCACTCGCGGTGCGGGCGTAGGTTCTCTTGTTGCCGTGTTCGATGGAAAGCCCACCGCCCACCAACAgg                                                >  1:5143/1‑139 (MQ=255)

                                                                               gTCACGCTGAGTTGTCACGACGGCTTCGGCCACCGCCAGCGTATGCTCCAGCGGTAGATAGCCAAACTCACTCGCGGTGCGGGCGTAGGTTTTCTTGTTGCCGTGTTCGATGGAAAGCCCACCGCCCACCAACAGGTTa                                             >  2:172281/1‑139 (MQ=255)

                                                                                    gCTGAGTTGTCACGACGGCTTCGGCCACCGCCAGCGTATGCTCCAGCGGTAGATAGCCAAACTCACTCGCGGTGCGGGCGTAGGTTTTCTTGTTGCCGTGTTCGATGGAAAGCCCACCGCCCACCAACAGGTTAAAGcc                                        >  1:389438/1‑139 (MQ=255)

                                                                                     cTGAGTTGTCACGACGGCTTCGGCCACCGCCAGCGTATGCTCCAGCGGTAGa                                                                                                                              >  1:293619/1‑52 (MQ=255)

                                                                                     cTGAGTTGTCACGACGGCTTCGGCCACCGCCAGCGTATGCTCCAGCGGTAGa                                                                                                                              <  2:293619/52‑1 (MQ=255)

                                                                                        aGTTGTCACGACGGCTTCGGCCACCGCCAGCGTATGCTCCAGCGGTAGATAGCCAAACTCACTCGCGGTGCGGGCGTAGGttt                                                                                            <  1:754403/83‑1 (MQ=255)

                                                                                        aGTTGTCACGACGGCTTCGGCCACCGCCAGCGTATGCTCCAGCGGTAGATAGCCAAACTCACTCGCGGTGCGGGCGTAGGttt                                                                                            >  2:754403/1‑83 (MQ=255)

                                                                                          ttGTCACGACGGCTTCGGCCACCGCCAGCGTATGCTCCAGCGGTAGATAGCCAAACTCACTCGCGGTGCGGGCGTAGGTTTTCTTGTTGCCGTGTTCGATGGAAAgcccaccgcccac                                                       <  2:232061/118‑1 (MQ=255)

                                                                                          ttGTCACGACGGCTTCGGCCACCGCCAGCGTATGCTCCAGCGGTAGATAGCCAAACTCACTCGCGGTGCGGGCGTAGGTTTTCTTGTTGCCGTGTTCGATGGAAAgcccaccgcccac                                                       >  1:232061/1‑118 (MQ=255)

                                                                                              cACGACGGCTTCGGCCACCGCCAGCGTATGCTCCAGCGGTAGATAGCCAAACTCACTCGCGGTGCGGGCGTAGGTTTTCTTGTTGCCGTGTTCGATGGAAAGCCCACCGCCCACCAACAGGTTAAAGCCCACCAGCTTg                              <  1:172281/139‑1 (MQ=255)

                                                                                                  acgGCTTCGGCCACCGCCAGCGTATGCTCCAGCGGTAGATAGCCAAACTCACTCGCGGTGCGGGCGTAGGTTTTCTTGTTGCCGTGTTCGATGGAAAGCCCACCGCCCACCAACAGGTTAAAGCCCACCAGCTTGCCGt                          >  1:611353/1‑139 (MQ=255)

                                                                                                           gCCACCGCCAGCGTATGCTCCAGCGGTAGATAACCAAACTCACTCGCGGTGCGGGCGTAGGTTTTCTTGTTGCCGTGTTCGATGGAAAGCCCACCGCCCACCAACAGGTTAAAGCCCACCAGCTTGCCGTTTTCGGCGa                 <  1:671118/139‑1 (MQ=255)

                                                                                                                  ccAGCGTATGCTCCAGCGGTAGATAGCCAAACTCACTCGCGGTGCGGGCGTAGGTTTTCTTGTTGCCGTGTTCGATGGAAAGCCCACCGCCCACCAACAGGTTAAAGCCCACCAGCTTGCCGTTTTCGGCGATCGCCAc          <  2:611353/139‑1 (MQ=255)

                                                                                                                   cAGCGTATGCTCCAGCGGTAGATAGCCAAACTCACTCGCGGTGCGGGCGTAGGTTTTCTTGTTGCCGTGTTCGATGGAAAGCCCACCGCCCACCAACAGGTTAAAGCCCACCAGCTTGCCGTTTTCGGCGATCGCCACg         <  1:373990/139‑1 (MQ=255)

                                                                                                                          tGCTCCAGCGGTAGATAGCCAAACTCACTCGCGGTGCGGGCGTAGGTTTTCTTGTTGCCGTGTTCGATGGAAAGCCCACCGCCCACCAACAGGTTAAAGCCCACCAGCTTGCCGTTTTCGGCGATCGCCACGAAGTTCa  <  2:389438/139‑1 (MQ=255)

CCGCTTTAAACGTCTCAACCCCCACGCGCTCCAGCGTGTATTTGGTTTTGGCATTTTTACGATCGGTTCGGTTACCCCAGTCACGCTGAGTTGTCACGACGGCTTCGGCCACCGCCAGCGTATGCTCCAGCGGCAGATAGCCAAACTCACTCGCGGTGCGGGCGTAGGTTTTCTTGTTGCCGTGTTCGATGGAAAGCCCACCGCCCACCAACAGGTTAAAGCCCACCAGCTTGCCGTTTTCGGCGATCGCCACGAAGTTCA  >  NC_000913/2889136‑2889396

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A