Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F1 I1 R1 65 25.3 1027584 98.0% 1007032 120.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,973,169 G→A G57G (GGC→GGT)  cheW ← purine‑binding chemotaxis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,973,1690GA100.0% 68.7 / NA 21G57G (GGC→GGT) cheWpurine‑binding chemotaxis protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/10);  total (11/10)

TCCGAGATTCAGGACGATAACTACCGTGTTGTCGTTATAGTCCACATCCACCTGGCTGAACTTAATTCGTAAGTCAACAATCGGCACAATAACGCCGCGCAGATTCGTGACGCCTTTGATAAACGCTGGCGTGTTCGCAATCCGTGTTACCTGATCGTAGCCACGGATCTCCTGCACTTTCAGGATATCAATACCGTACTCTTCATCACCAAGGGTAAATACCAGAAATTCCTGGCCTGAC  >  NC_000913/1973058‑1973298
                                                                                                               |                                                                                                                                 
tCCGAGATTCAGGACGATAACTACCGTGTTGTCGTTATAGTCCACATCCACCTGGCTGAACTTAATTCGTAAGTCAACAATCGGCACAATAACGCCGCGCAGATTCGTGACACCTTTGATAAACGCTGGCGTGTTCGCa                                                                                                        >  1:474543/1‑139 (MQ=255)
 ccGACATTCAGGACGATAACTACCGTGTTGTCGTTACAGTCCACATCCACCTGGCTGAACTTAATTCGTAAGCCAACAATCGGCACAATAACGCCGCGCAGATTCGTGACACCTTTGATAAACGCTGGCGTGTTCGCaa                                                                                                       <  1:321061/139‑1 (MQ=255)
   gagaTTCAGGACGATAACTACCGTGTTGTCGTTATAGTCCACATCCACCTGGCTGAACTTAATTCGTAAGTCAACAATCGGCACAATAACGCCGCGCAGATTCGTGACACCTTTGATAAACGCTGGCGTGTTCGCAATc                                                                                                     <  2:118104/139‑1 (MQ=255)
    agaTTCAGGACGATAACTACCGTGTTGTCGTTATAGTCCACATCCACCTGGCTGAACTTAATTCGTAAGTCAACAATCGGCACAATAACGCCGCGCAGATTCGTGACACCTTTGATAAACGCTGGCGTGTTCGCAATcc                                                                                                    >  1:76012/1‑139 (MQ=255)
                                        tCCACATCCACCTGGCTGAACTTAATTCGTAAGTCAACAATCGGCACAATAACGCCGCGCAGATTCGTGACACCTTTGATAAACGCTGGCGTGTTCGCAATCCGTGTTACCTGATCGTAGCCACGGATCTCCTGCACtt                                                                >  2:66170/1‑139 (MQ=255)
                                         ccACATCCACCTGGCTGAACTTAATTCGTAAGTCAACAATCGGCACAATAACGCCGCGCAGATTCGTGACACCTTTGATAAACGCTGGCGTGTTCGCAATCCGTGTTACCTGATCGTAGCCACGGATCTCCTGCACttt                                                               >  2:491477/1‑139 (MQ=255)
                                            caTCCACCTGGCCGAACTTAATTCGTAAGTCAACAATCGGCACAATAACGCCGCGCAGATTCGTGACACCTTTGATAAACGCTGGCGTGTTCGCAATCCGTGTTACCTGATCGTAGCCACGGATCTCCTGCACTTTCAg                                                            >  1:265625/1‑139 (MQ=255)
                                                 aCCTGGCTGAACTTAATTCGTAAGTCAACAATCGGCACAATAACGCCGCGCAGATTCGTGACACCTTTGATAAACGCTGGCGTGTTCGCAATCCGTGTTACCTGATCGTAGCCACGGATCTCTTGCACTTTCAGGatat                                                       <  2:63407/139‑1 (MQ=255)
                                                 aCCTGGCTGAACTTAATTCGTAAGTCAACAATCGGCACAATAACGCCGCGCAGATTCGTGACACCTTTGATAAACGCTGGCGTGTTCGCAATCCGTGTTACCTGATCGTAGCCACGGATCTCCTGCACTTTCAGGatat                                                       <  1:350822/139‑1 (MQ=255)
                                                  ccTGGCTGAACTTAATTCGTAAGTCAACAATCGGCACAATAACGCCGCGCAGATTCGTGACACCTTTGATAAACGCTGGCGTGTTCGCAATCCGTGTTACCTGATCGTAGCCACGGATCTCCTGCACTTTCAGGATATc                                                      >  1:352223/1‑139 (MQ=255)
                                                  ccTGGCTGAACTTAATTCGTAAGTCAACAATCGGCACAATAACGCCGCGCAGATTCGTGACACCTTTGATAAACGCTGGCGTGTTCGCAATCCGTGTTACCTGATCGTAGCCACGGATCTCCTGCACTTTCAGGATATc                                                      >  2:259304/1‑139 (MQ=255)
                                                         gAACTTAATTCGTAAGTCAACAATCGGCACAATAACGCCGCGCAGATTCGTGACACCTTTGATAAACGCTGGCGTGTTCGCAATCCGTGTTACCTGATCGTAGCCACGGATCTCCTGCACTTTCAGGATATCAATACCg                                               >  2:254526/1‑139 (MQ=255)
                                                            cTTAATTCGTAAGTCAACAATCGGCACAATAACGCCGCGCAGATTCGTGACACCTTTGATAAACGCTGGCGTGTTCGCAATCCGTGTTACCTGATCGTAGCCACGGATCTCCTGCACTTTCAGGATATCAATACCGTAc                                            >  1:47645/1‑139 (MQ=255)
                                                                aTTCGTAAGTCAACAATCGGCACAATAACGCCGCGCAGATTCGTGACACCTTTGATAAACGCTGGCGTGTTCGCAATCCGTGTTACCTGATCGTAGCCACGGATCTCCTGCACTTTCAGGATATCAATa                                                  >  2:378724/1‑129 (MQ=255)
                                                                aTTCGTAAGTCAACAATCGGCACAATAACGCCGCGCAGATTCGTGACACCTTTGATAAACGCTGGCGTGTTCGCAATCCGTGTTACCTGATCGTAGCCACGGATCTCCTGCACTTTCAGGATATCAATa                                                  <  1:378724/129‑1 (MQ=255)
                                                                  tCGTAAGTCAACAATCGGCACAATAACGCCGCGCAGATTCGTGACACCTTTGATAAACGCTGGCGTGTTCGCAATCCGTGTTACCTGATCGTATCCACGGATCTCCTGCACTTTCAGGATATCAATACCGTACTCTtca                                      <  1:378277/139‑1 (MQ=255)
                                                                       aGTCAACAATCGGCACAATAACGCCGCGCAGATTCGTGACACCTTTGATAAACGCTGGCGTGTTCGCAATCCGTGTTACCTGATCGTAGCCACGGATCTCCTGCACTTTCAGGATATCAATACCGTACTCTTCATCAcc                                 <  1:322638/139‑1 (MQ=255)
                                                                             caaTCGGCGCAATAACGCCGCGCAGATTCGTGACACCTTTGGTAAACGCTGGCGTGTTCGCAATCCGTGTTACCTGATCGTAGCCACGGATCTCCTGCACTTTc                                                              <  1:333126/104‑1 (MQ=255)
                                                                             caaTCGGCGCAATAACGCCGCGCAGATTCGTGACACCTTTGATAAACGCTGGCGTGTTCGCAATCCGTGTTACCTGATCGTAGCCACGGATCTCCTGCACTTTc                                                              >  2:333126/1‑104 (MQ=255)
                                                                                               cgcgCAGATTCGTGACACCTTTGATAAACGCTGGCGTGTTCGCAATCCGTGTTACCTGATCGTAGCCACGGATCTCCTGCACTTTCAGGATATCAATACCGTACTCTTCATCACCAAGGGTAAATACCAGAAATTCCTg         <  2:265625/139‑1 (MQ=255)
                                                                                                      aTTCGTGACACCTTTGATAAACGCTGGCGTGTTCGCAATCCGTGTTACCTGATCGTAGCCACGGATCTCCTGCACTTTCAGGATATCAATACCGTACTCTTCATCACCAAGGGTAAATACCAGAAATTCCTGGCCTGAc  <  2:474543/139‑1 (MQ=255)
                                                                                                               |                                                                                                                                 
TCCGAGATTCAGGACGATAACTACCGTGTTGTCGTTATAGTCCACATCCACCTGGCTGAACTTAATTCGTAAGTCAACAATCGGCACAATAACGCCGCGCAGATTCGTGACGCCTTTGATAAACGCTGGCGTGTTCGCAATCCGTGTTACCTGATCGTAGCCACGGATCTCCTGCACTTTCAGGATATCAATACCGTACTCTTCATCACCAAGGGTAAATACCAGAAATTCCTGGCCTGAC  >  NC_000913/1973058‑1973298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A