Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F1 I1 R1	65	25.3	1027584	98.0%	1007032	120.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,250,649	C→T	H311H (CAC→CAT)	yeiH →	UPF0324 family inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,250,649	0	C	T	100.0%	111.1 / NA	32	H311H (CAC→CAT)	yeiH	UPF0324 family inner membrane protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (14/18); total (14/18)

TTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCATAAACCACTACATCTTGC  >  NC_000913/2250515‑2250781

tttGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCa                                                                                                                                  >  1:132616/1‑139 (MQ=255)

tttGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCa                                                                                                                                  >  1:350445/1‑139 (MQ=255)

              cATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGaaaaaa                                                                                                                    >  2:117081/1‑139 (MQ=255)

                   tAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTgg                                                                                                               >  1:278357/1‑139 (MQ=255)

                        gCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGa                                                                                                          <  2:264364/139‑1 (MQ=255)

                             cTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACct                                                                                                     <  2:303428/139‑1 (MQ=255)

                                tAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTctg                                                                                                  <  1:305083/139‑1 (MQ=255)

                                        tCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGc                                                                                          >  1:142327/1‑139 (MQ=255)

                                                          gCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTg                                                                        >  2:417801/1‑139 (MQ=255)

                                                                               cGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGc                                                   <  1:209203/139‑1 (MQ=255)

                                                                                gCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCg                                                  <  2:142327/139‑1 (MQ=255)

                                                                                 cTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTctg                                                                                                  <  2:444650/90‑1 (MQ=255)

                                                                                 cTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTctg                                                                                                  >  1:444650/1‑90 (MQ=255)

                                                                                 cTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGa                                                 >  1:55437/1‑139 (MQ=255)

                                                                                     aTACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTtggtggtg                                                       <  1:13797/129‑1 (MQ=255)

                                                                                     aTACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTtggtggtg                                                       >  2:13797/1‑129 (MQ=255)

                                                                                     aTACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTaa                                             <  2:53531/139‑1 (MQ=255)

                                                                                       aCCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACt                                           <  2:350445/139‑1 (MQ=255)

                                                                                         cTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTAt                                         <  1:193123/139‑1 (MQ=255)

                                                                                           tCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGAt                                                >  1:438466/1‑130 (MQ=255)

                                                                                           tCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGAt                                                <  2:438466/130‑1 (MQ=255)

                                                                                           tCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTAtgt                                       >  2:223535/1‑139 (MQ=255)

                                                                                              tgctgGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAgcgcg                                                                                                                             <  1:378934/50‑1 (MQ=255)

                                                                                              tgctgGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAgcgcg                                                                                                                             >  2:378934/1‑50 (MQ=255)

                                                                                                          cgatggcgGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCg                                                  <  1:513452/113‑1 (MQ=255)

                                                                                                          cgatggcgGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCg                                                  >  2:176068/1‑113 (MQ=255)

                                                                                                          cgatggcgGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCg                                                  <  1:176068/113‑1 (MQ=255)

                                                                                                          cgatggcgGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCg                                                  >  2:513452/1‑113 (MQ=255)

                                                                                                                  gcgcTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCATaaa                <  2:55437/139‑1 (MQ=255)

                                                                                                                            tGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCATAAACCACTACATc      <  1:417801/139‑1 (MQ=255)

                                                                                                                                cACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGACACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCATAAACCACTACATCTTGc  <  2:189548/139‑1 (MQ=255)

                                                                                                                                   tCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTtggtggtg                                                       <  1:342394/83‑1 (MQ=255)

TTTGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCATAAACCACTACATCTTGC  >  NC_000913/2250515‑2250781

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A