Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F1 I1 R1	81	47.9	2225048	94.8%	2109345	113.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,401,103	G→A	T151T (ACC→ACT)	nuoF ←	NADH:ubiquinone oxidoreductase, chain F

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,401,103	0	G	A	100.0%	129.9 / NA	38	T151T (ACC→ACT)	nuoF	NADH:ubiquinone oxidoreductase, chain F
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (19/19); total (19/19)

GGTTAGCACGACGTCCTTCCAGGGAGTTGATTAACGCTGTTTCTTCCCCGCAGATGTAGCGCCCTGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCGGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCACGGTAAGCTTTCAGCGCAAACGCGGAGATGAGCATACCTTCCACCAGCAGGTGCGGCAGTTG  >  NC_000913/2400967‑2401237

ggTTAGCACGACGTCCTTCCAGGGAGTTGATTAACGCTGTTTCTTCCCCGCAGATGTAGCGCCCTGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGt                                                                                                                                      <  2:336161/139‑1 (MQ=255)

    aGCACGACGTCCTTCCAGGGAGTTGATTAACGCTGTTTCTTCCCCGCAGATGTAGCGCCCTGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGGCt                                                                                                                                  <  2:319519/139‑1 (MQ=255)

                ttCCAGGGAGTTGATTAACGCTGTTTCTTCCCCGCAGATGTAGCGCCCTGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCa                                                                                                                      <  2:823656/139‑1 (MQ=255)

                          ttGATTAACGCTGTTTCTTCCCCGCAGATGTAGCGCCCTGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGAt                                                                                                            >  1:859259/1‑139 (MQ=255)

                                  cGCTGTTTCTTCCCCGCAGATGTAGCGCCCTGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGcc                                                                                                    >  1:9214/1‑139 (MQ=255)

                                     tGTTTCTTCCCCGCAGATGTAGCGCCCTGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCt                                                                                                 <  1:1006981/139‑1 (MQ=255)

                                     tGTTTCTTCCCCGCAGATGTAGCGCCCTGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCt                                                                                                 <  2:859259/139‑1 (MQ=255)

                                       tttCTTCCCCGCAGATGTAGCGCCCTGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTc                                                                                               >  1:1068151/1‑139 (MQ=255)

                                            tcCCCGCAGATGTAGCGCCCTGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGatat                                                                                          >  1:735433/1‑139 (MQ=255)

                                                             cccTGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAg                                                                         >  1:657355/1‑139 (MQ=255)

                                                                tGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACgg                                                                                                                   >  2:953356/1‑94 (MQ=255)

                                                                tGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACgg                                                                                                                   <  1:953356/94‑1 (MQ=255)

                                                                tGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATg                                                                      <  2:613627/139‑1 (MQ=255)

                                                                  ccccGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCGGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTa                                                                    >  2:567864/1‑139 (MQ=255)

                                                                  ccccGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTa                                                                    >  1:841261/1‑139 (MQ=255)

                                                                       gTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCAc                                                               <  1:567864/139‑1 (MQ=255)

                                                                            gACGAACAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGt                                                                                                                                      <  2:1099033/63‑1 (MQ=255)

                                                                            gACGAACAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCt                                                                                                 <  2:299602/100‑1 (MQ=255)

                                                                            gACGAACAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCt                                                                                                 >  1:299602/1‑100 (MQ=255)

                                                                            gACGAACAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCACGGTaa                                                          >  2:68321/1‑139 (MQ=255)

                                                                                 aCAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCACGGTAAGCttt                                                     >  1:851936/1‑139 (MQ=255)

                                                                                 aCAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCACGGTAAGCttt                                                     >  2:946352/1‑139 (MQ=255)

                                                                                  cAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGGCtt                                                                                                                                 <  2:99860/62‑1 (MQ=255)

                                                                                  cAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGGCtt                                                                                                                                 >  1:99860/1‑62 (MQ=255)

                                                                                  cAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGgc                                                                                                                  >  2:612499/1‑77 (MQ=255)

                                                                                  cAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGgc                                                                                                                  <  1:612499/77‑1 (MQ=255)

                                                                                  cAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGcc                                                                                   <  2:316273/108‑1 (MQ=255)

                                                                                  cAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGcc                                                                                   >  1:316273/1‑108 (MQ=255)

                                                                                  cAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCACGGTAAGCTTTc                                                    >  2:103256/1‑139 (MQ=255)

                                                                                               aaaCCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCACGGTAAGCTTTCAGCGCAAACGCgg                                       >  2:405925/1‑139 (MQ=255)

                                                                                                aaCCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCACGGTAAGCTTTCAGCGCAAACGCGga                                      <  1:946352/139‑1 (MQ=255)

                                                                                                 aCCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCACGGTAAGCTTTCAGCGCAAACGCGgag                                     <  1:940941/139‑1 (MQ=255)

                                                                                                 aCCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCACGGTAAGCTTTCAGCGCAAACGCGgag                                     <  2:851936/139‑1 (MQ=255)

                                                                                                             aaTGTTTTTGCCAAGCAGACCCGCTTCAGTGGCtt                                                                                                                                 >  2:1103405/1‑35 (MQ=255)

                                                                                                             aaTGTTTTTGCCAAGCAGACCCGCTTCAGTGGCtt                                                                                                                                 <  1:1103405/35‑1 (MQ=255)

                                                                                                              aTGTTTTTGCCAAGCAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCACGGTAAGCTTTCAGCGCAAACGCGGAGATGAGCATACCtt                        <  1:103256/139‑1 (MQ=255)

                                                                                                                            cAGACCCGCTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCACGGTAAGCTTTCAGCGCAAACGCGGAGATGAGCATACCTTCCACCAGCAGGTGc          <  2:21594/139‑1 (MQ=255)

                                                                                                                                    cTTCAGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCACGGTAAGCTTTCAGCGCAAACGCGGAGATGAGCATACCTTCCACCAGCAGGTGCGGCAGTTg  >  2:759283/1‑139 (MQ=255)

GGTTAGCACGACGTCCTTCCAGGGAGTTGATTAACGCTGTTTCTTCCCCGCAGATGTAGCGCCCTGCCCCGGTATGGACGAACAGTTCGAAATCGAAACCTGTTCCCATAATGTTTTTGCCAAGCAGACCCGCTTCGGTGGCTTCGGCAATGGCACGGCGCAGATTAACTGCCGCTTCGATATATTCGCCACGCAGGAAGATGTAGCCACGGTAAGCTTTCAGCGCAAACGCGGAGATGAGCATACCTTCCACCAGCAGGTGCGGCAGTTG  >  NC_000913/2400967‑2401237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A