Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F1 I1 R1	81	47.9	2225048	94.8%	2109345	113.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,709,561	(C)_6→7	coding (915/1692 nt)	eptB ←	KDO phosphoethanolamine transferase, Ca(2+)‑inducible

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,709,555	1	.	C	100.0%	170.3 / NA	46	G307G (GGC→GGG)	eptB	KDO phosphoethanolamine transferase, Ca(2+)‑inducible
Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (21/25); total (21/25)

ATGTTGTCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CG‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGAAAATAC  >  NC_000913/3709421‑3709685

aTGTTGTCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTACTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CG‑cccc                                                                                                                                >  1:518852/1‑139 (MQ=255)

     gtCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CG‑ccccccctg                                                                                                                           <  2:1106425/139‑4 (MQ=255)

       ccGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CG‑ccccccctgac                                                                                                                         >  1:370508/1‑134 (MQ=255)

       ccGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CG‑ccccccctg                                                                                                                           <  1:53579/137‑4 (MQ=255)

       ccGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CG‑ccccccctg                                                                                                                           >  2:53579/1‑134 (MQ=255)

        cGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCcg‑cg                                                                                                                                     <  1:933956/127‑1 (MQ=255)

        cGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCcg‑cg                                                                                                                                     >  2:933956/1‑127 (MQ=255)

        cGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACg                                                                                                                         <  1:798943/139‑1 (MQ=255)

        cGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACg                                                                                                                         <  2:370508/139‑1 (MQ=255)

                        tAGAAGCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCCAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTTTCTTCCG‑CGCCCCCCCTGGCGAACAACCATGCAACGc                                                                                                         <  1:742795/139‑1 (MQ=255)

                             ccACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTga                                                                                                    <  2:475540/139‑1 (MQ=255)

                                         ctGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGc                                                                                        <  2:822280/139‑1 (MQ=255)

                                              tAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTAt                                                                                   <  2:76723/139‑1 (MQ=255)

                                                 cGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATcac                                                                                >  2:925144/1‑139 (MQ=255)

                                                    agagGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAgg                                                                             >  1:109766/1‑139 (MQ=255)

                                                      agGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACg                                                                                                          <  1:1100127/108‑1 (MQ=255)

                                                      agGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACg                                                                                                          >  2:1100127/1‑108 (MQ=255)

                                                      agGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAg                                                                           >  1:81927/1‑139 (MQ=255)

                                                           aGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAAcc                                                                      >  1:326199/1‑139 (MQ=255)

                                                           aGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAAcc                                                                      >  2:207877/1‑139 (MQ=255)

                                                                  aaTCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGc                                                                                                              <  2:197153/92‑1 (MQ=255)

                                                                  aaTCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGc                                                                                                              >  1:197153/1‑92 (MQ=255)

                                                                  aaTCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGt                                                                                                      >  2:751919/1‑100 (MQ=255)

                                                                  aaTCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGt                                                                                                      <  1:751919/100‑1 (MQ=255)

                                                                  aaTCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAAc                                                               >  2:618145/1‑139 (MQ=255)

                                                                  aaTCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAAc                                                               >  2:980315/1‑139 (MQ=255)

                                                                  aaTCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CG‑ccccccct                                                                                                                            >  2:1079877/1‑75 (MQ=255)

                                                                  aaTCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CG‑ccccccct                                                                                                                            <  1:1079877/77‑3 (MQ=255)

                                                                       tAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAAcgccgc                                                          >  1:402850/1‑139 (MQ=255)

                                                                         aCTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGTAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCa                                                        <  1:388121/139‑1 (MQ=255)

                                                                            gCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTg                                                                                         <  1:260695/103‑1 (MQ=255)

                                                                            gCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTg                                                                                         >  2:260695/1‑103 (MQ=255)

                                                                                    aCCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTcc                                             <  1:340126/139‑1 (MQ=255)

                                                                                        cgAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTggg                                         >  1:898915/1‑139 (MQ=255)

                                                                                         gAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGc                                        <  2:783761/139‑1 (MQ=255)

                                                                                               gttctgttctTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGttt                                  <  1:980315/139‑1 (MQ=255)

                                                                                                    gttctTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCg                             <  1:618145/139‑1 (MQ=255)

                                                                                                    gttctTTTAATGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCg                             <  1:698041/139‑1 (MQ=255)

                                                                                                            aaTGTGCGCTGCGGATTATCTTCCGCCA‑CCCCCCTGACGTACAAACATGCAACGCAGGGAGAGTTTGCTTGCGGTGTCACAGGTGTAACTACGGAACGACGCCAAACTTTCCTCCTGGGCGAGTTTCGGCGGGATATTa                     <  1:420484/139‑1 (MQ=255)

                                                                                                            aaTGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTa                     <  1:925144/139‑1 (MQ=255)

                                                                                                             aTGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCg                             <  1:590673/130‑1 (MQ=255)

                                                                                                             aTGTGCGCTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCg                             >  2:590673/1‑130 (MQ=255)

                                                                                                                 gcgcTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTc                <  2:898915/139‑1 (MQ=255)

                                                                                                                  cgcTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATTCAACGCTGTGAAGTTTTGGTTGCGGTATCACAGTAGTAACCACGTAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCa               >  1:944789/1‑139 (MQ=255)

                                                                                                                  cgcTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGc                                                                                                              >  1:411012/1‑44 (MQ=255)

                                                                                                                  cgcTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGc                                                                                                              <  2:411012/44‑1 (MQ=255)

                                                                                                                  cgcTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATc                                                                                  <  2:135827/72‑1 (MQ=255)

                                                                                                                  cgcTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATc                                                                                  >  1:135827/1‑72 (MQ=255)

                                                                                                                  cgcTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCa               >  2:188269/1‑139 (MQ=255)

                                                                                                                  cgcTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCa               >  2:862264/1‑139 (MQ=255)

                                                                                                                  cgcTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCa               >  1:841799/1‑139 (MQ=255)

                                                                                                                  cgcTGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAATGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCa               >  2:650772/1‑139 (MQ=255)

                                                                                                                     tGCGGATTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAg            <  1:188269/139‑1 (MQ=255)

                                                                                                                          aTTATCTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGaa       <  2:633528/139‑1 (MQ=255)

                                                                                                                               cTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGAAAATAc  <  2:402850/139‑1 (MQ=255)

                                                                                                                               cTTCCG‑CGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGAAAATAc  <  2:81927/139‑1 (MQ=255)

ATGTTGTCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCG‑CG‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGAAAATAC  >  NC_000913/3709421‑3709685

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A