Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F1 I1 R1
|
81 |
47.9 |
2225048 |
94.8% |
2109345 |
113.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,606,215 |
(G)8→9 |
noncoding (72/87 nt) |
leuP ← |
tRNA‑Leu |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,606,207 | 1 | . | G | 89.5%
| 119.9
/ 7.5
| 38 | noncoding (80/87 nt) | leuP | tRNA‑Leu |
| Reads supporting (aligned to +/- strand): ref base . (2/2); new base G (16/18); total (18/20) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.38e-01 |
GTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑G‑GGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTG > NC_000913/4606081‑4606335
|
gTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑g‑ggg‑gggggact > 1:309327/1‑135 (MQ=255)
tGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑g‑ggg‑gggggactt < 2:125211/139‑6 (MQ=255)
gggggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGG‑GGG‑GGGGACTTGAAccccc < 2:309327/139‑1 (MQ=255)
ggggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGG‑GGG‑GGGGACTTGAACCCCCa > 1:11385/1‑139 (MQ=255)
ggggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑g‑ggg‑gg < 2:436713/123‑1 (MQ=255)
ggggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑g‑ggg‑gg > 1:436713/1‑123 (MQ=255)
gggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGG‑GGG‑GGGGACTTGAACCCCCAc > 2:771523/1‑139 (MQ=255)
ggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGG‑GGG‑GGGGACTTGAACCCCCACg < 1:771523/139‑1 (MQ=255)
ttGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑g‑ggg‑gg > 2:920240/1‑117 (MQ=255)
ttGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑g‑ggg‑gg < 1:920240/117‑1 (MQ=255)
tGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGG‑GGG‑GGGGACTTGAACCCCCACGTCCGt < 2:532697/139‑1 (MQ=255)
acacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGG‑GGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGc < 2:491852/139‑1 (MQ=255)
acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGG‑GGG‑GGGGACTTGAACCCCCACGTCCGTAAGAac > 2:905209/1‑122 (MQ=255)
acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGG‑GGG‑GGGGACTTGAACCCCCACGTCCGTAAGAac < 1:905209/122‑1 (MQ=255)
tAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGG‑GGG‑GGGGCCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgc > 2:699361/1‑139 (MQ=255)
aaCACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGG‑GGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgcg < 2:218041/139‑1 (MQ=255)
aaCACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTAGCACAGTCGTCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGG‑GGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgcg < 1:246932/139‑1 (MQ=255)
acCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑G‑GGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTc > 2:728290/1‑139 (MQ=255)
ccTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGG‑GGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTc > 2:1093629/1‑139 (MQ=255)
gAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGG‑GGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTAc > 2:8978/1‑139 (MQ=255)
cgcgTCTACCAATTCCGCCACCTTCGCACAGTCCTCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGG‑GGG‑GGGGCCTTGAACCCCCACGTCCGTAAAAAAACTAACACCTGAAGCTAGCGCGTCTACCAATTCCg > 1:914987/1‑139 (MQ=25)
gTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑g‑ggg‑gggggactt > 1:866745/1‑74 (MQ=255)
gTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑g‑ggg‑gggggactt < 2:866745/79‑6 (MQ=255)
cTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑g‑ggg‑gggggac > 2:708810/1‑72 (MQ=255)
cTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑g‑ggg‑gggggac < 1:708810/75‑4 (MQ=255)
aTTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGG‑GGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgcgc > 1:851461/1‑114 (MQ=255)
aTTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGG‑GGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgcgc < 2:851461/114‑1 (MQ=255)
aTTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGG‑GGG‑GGGGACTTGAACCCCAACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc < 1:1093629/139‑1 (MQ=255)
aTTCCGCCAACTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGG‑GGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc < 2:1096606/139‑1 (MQ=255)
gCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGG‑GGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTg < 2:11385/139‑1 (MQ=255)
ccACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGG‑GGG‑GGGGACTTGAACCCCCACGCCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGtac > 1:295151/1‑137 (MQ=255)
cACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGG‑GGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCg < 1:194348/139‑1 (MQ=255)
tCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑G‑GGGTGGGGACTTGAACCCCCACg > 2:981769/1‑70 (MQ=255)
tCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑G‑GGGTGGGGACTTGAACCCCCACg < 1:981769/70‑1 (MQ=255)
caGTCATCATACTTTTTTTGATATCGCCTCGTTTGGTGCGAGG‑GGG‑GGGGACTTGAACCCCCATGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCa < 2:263176/139‑1 (MQ=255)
aGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑G‑GGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAAc < 1:728290/139‑1 (MQ=255)
ttCATCTTACTTTGTTTGATATCGACTCGTTTGGTGCGA‑GTGCG‑GGGGACTTGTACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAAc < 1:699361/138‑1 (MQ=21)
gTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGG‑GGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACAGATGAATCAAGAGCGTCTACCAATTCCGCCACATTCGCAAAGTGCGAGCAATATAAAc > 1:80201/1‑139 (MQ=18)
aTCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGG‑GGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCg < 1:907787/124‑1 (MQ=255)
aTCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGG‑GGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCg > 2:907787/1‑124 (MQ=255)
tCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGG‑GGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACAcc > 1:360211/1‑79 (MQ=255)
tCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGG‑GGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACAcc < 2:360211/79‑1 (MQ=255)
ttttttGATATCGCCTCGTTTGGTGCGAGG‑GGG‑GGGGACTTGa > 1:137238/1‑43 (MQ=37)
ttttttGATATCGCCTCGTTTGGTGCGAGG‑GGG‑GGGGACTTGa < 2:137238/43‑1 (MQ=37)
tttGGTGCGAGG‑GGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTg > 1:104699/1‑139 (MQ=255)
tGGTGCGAGG‑GGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTg > 1:832513/1‑94 (MQ=255)
tGGTGCGAGG‑GGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTg < 2:832513/94‑1 (MQ=255)
gTGCGAGG‑GGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTg > 2:114121/1‑92 (MQ=255)
gTGCGAGG‑GGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTg < 1:114121/92‑1 (MQ=255)
|
GTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑G‑GGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTG > NC_000913/4606081‑4606335
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
