Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F1 I2 R1 78 65.3 3016648 94.6% 2853749 111.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,107,866 T→C Q155R (CAG→CGG)  glf ← UDP‑galactopyranose mutase, FAD/NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,107,8660TC100.0% 231.5 / NA 63Q155R (CAG→CGG) glfUDP‑galactopyranose mutase, FAD/NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/32);  total (31/32)

TCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAATCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATAAGTCCTCCCCAACTAATGAAATCGCCTGCTCCTCCAAATTTTCAGGTACCTTGTCACCATACTTTTTTTTCTGAGCATTAATGATATTTTGAG  >  NC_000913/2107734‑2107995
                                                                                                                                    |                                                                                                                                 
tCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAATCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACCGCTtct                                                                                                                             <  2:103483/139‑1 (MQ=255)
      aGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAATCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACCGCTTCTCCGtat                                                                                                                       <  1:1473488/139‑1 (MQ=255)
      aGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAATCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACCGCTTCTCCGtat                                                                                                                       <  1:1398385/139‑1 (MQ=255)
           aGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAATCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACCGCTTCTCCGTATAAccc                                                                                                                  <  2:1246812/139‑1 (MQ=255)
                  ccTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAATCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACCGCTTCTCCGTATAACCCTTTATCa                                                                                                           <  1:627604/139‑1 (MQ=255)
                  ccTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAATCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACCGCTTCTCCGTATAACCCTTTATCa                                                                                                           >  2:1401185/1‑139 (MQ=255)
                  ccTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAATCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACCGCTTCTCCGTATAACCCTTTATCa                                                                                                           <  2:1299420/139‑1 (MQ=255)
                     cccACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAATCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACCGCTTCTCCGTATAACCCTTTATCAATg                                                                                                        <  1:682990/139‑1 (MQ=255)
                     cccACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAATCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACCGCTTCTCCGTATAACCCTTTATCAATg                                                                                                        >  1:1031405/1‑139 (MQ=255)
                       cACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAATCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACCGCTTCTCCGTATAACCCTTTATCAATGCt                                                                                                      <  1:1401185/139‑1 (MQ=255)
                           ggAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAATCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACCGCTTCTCCGTATAACCCTTTATc                                                                                                            <  2:749908/129‑1 (MQ=255)
                           ggAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAATCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACCGCTTCTCCGTATAACCCTTTATc                                                                                                            >  1:749908/1‑129 (MQ=255)
                            gAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAATCTCACTTGGTTTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACCGCTTCTCCGTATAACCCTTTATCAATGCt                                                                                                      <  2:956597/134‑1 (MQ=255)
                            gAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAATCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTGGCACTTCTTCCCCACCGCTTCTCCGTATAACCCTTTATCAATGCt                                                                                                      >  1:956597/1‑134 (MQ=255)
                                      aTAGCGATCGGAAAAATAATTGTTATCAAACGTAAATCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACCGCTTCTCCGTATAAccc                                                                                                                  <  1:126040/112‑1 (MQ=255)
                                      aTAGCGATCGGAAAAATAATTGTTATCAAACGTAAATCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACCGCTTCTCCGTATAAccc                                                                                                                  >  2:126040/1‑112 (MQ=255)
                                      aTAGCGATCGGAAAAATAATTGTTATCAAACGTAAATCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACCGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATAAGtcctcc                                                                                       >  1:795986/1‑139 (MQ=255)
                                       tAGCGATCGGAAAAATAATTGTTATCAAACGTAAATCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACCGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATAAGTCCTccc                                                                                      >  1:1249068/1‑139 (MQ=255)
                                            aTCGGAAAAATAATTGTTATCAAACGTAAATCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACCGCTTCTCCGTATAAccc                                                                                                                  <  1:847221/106‑1 (MQ=255)
                                            aTCGGAAAAATAATTGTTATCAAACGTAAATCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACCGCTTCTCCGTATAAccc                                                                                                                  >  2:847221/1‑106 (MQ=255)
                                            aTCGGAAAAATAATTGTTATCAAACGTAAATCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACCGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATAAGTCCTCCCCAACt                                                                                 >  1:1301444/1‑139 (MQ=255)
                                            aTCGGAAAAATAATTGTTATCAAACGTAAATCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACCGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATAAGTCCTCCCCAACt                                                                                 >  2:520951/1‑139 (MQ=255)
                                                  aaaaTAATTGTTATCAAACGTAAATCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACCGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATAAGTCCTCCCCAACTAATGaa                                                                           <  2:1217342/139‑1 (MQ=255)
                                                                 aaaCGTAAATCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACCGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATAAGTCCTCCCCAACTAATGAAATCGCCTGCtcctcc                                                            <  1:916335/139‑1 (MQ=255)
                                                                        aaTCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACCGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATAAGTCCTCCCCAACTAATGAAATCGCCTGCTCCTCCAAAtttt                                                     >  2:983318/1‑139 (MQ=255)
                                                                          tctcACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACCGCTTCTCCGTATAACCCTTTAt                                                                                                             >  2:1474779/1‑81 (MQ=255)
                                                                          tctcACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACCGCTTCTCCGTATAACCCTTTAt                                                                                                             <  1:1474779/81‑1 (MQ=255)
                                                                                    aTTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACCGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATAAGTCCTCCCCAACTAATGAAATCGCCTGCTCCTCCAAATTTTCAGGTACCTTGt                                         >  2:258554/1‑139 (MQ=255)
                                                                                    aTTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACCGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATAAGTCCTCCCCAACTAATGAAATCGCCTGCTCCTCCAAATTTTCAGGTACCTTGt                                         >  2:611271/1‑139 (MQ=255)
                                                                                    aTTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACCGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATAAGTCCTCCCCAACTAATGAAATCGCCTGCTCCTCCAAATTTTCAGGTACCTTGt                                         <  1:258554/139‑1 (MQ=255)
                                                                                                  aaaTGCTGGCAATTCTTTTGCACTTCTTCCCCACCGCTTCTCCGTATAAccc                                                                                                                  <  2:871027/52‑1 (MQ=255)
                                                                                                  aaaTGCAGGCAATTCTTTTGCACTTCTTCCCCACCGCTTCTCCGTATAAccc                                                                                                                  >  1:871027/1‑52 (MQ=255)
                                                                                                   aaTGCAGGCAATTCTTTTGCACTTCTTCCCCACCGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATAAGTCCTCCCCAACTAATGAAATCGCCTGCTCCTCCAAATTTTCAGGTACCTTGTCACCATACttttttt                          >  1:1206079/1‑139 (MQ=255)
                                                                                                    aTGCAGGCAATTCTTTTGCACTTCTTCCCCACCGCTTCTCCGTATAACCCTTTATCAATGc                                                                                                       <  2:922157/61‑1 (MQ=255)
                                                                                                    aTGCAGGCAATTCTTTTGCACTTCTTCCCCACCGCTTCTCCGTATAACCCTTTATCAATGc                                                                                                       >  1:1163887/1‑61 (MQ=255)
                                                                                                    aTGCAGGCAATTCTTTTGCACTTCTTCCCCACCGCTTCTCCGTATAACCCTTTATCAATGc                                                                                                       <  2:1163887/61‑1 (MQ=255)
                                                                                                    aTGCAGGCAATTCTTTTGCACTTCTTCCCCACCGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATAAGTCCTCCCCAACTAATGAAATCGCCTGCTCCTCCAAATTTTCAGGTACCTTGTCACCATACtttttttt                         <  1:520951/139‑1 (MQ=255)
                                                                                                    aTGCAGGCAATTCTTTTGCACTTCGTCCCCACCGCTTCTCCGTATAACCCTTTATCAATGc                                                                                                       >  1:922157/1‑61 (MQ=255)
                                                                                                       cAGGCAATTCTTTTGCACTTCTTCCCCACCGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATAAGTCCTCCCCAACTAATGAAATCGCCTGCTCCTCCAAATTTTCAGGTACCTTGTCACCATACTTTTTTTTCt                       >  1:19285/1‑138 (MQ=255)
                                                                                                       cAGGCAATTCTTTTGCACTTCTTCCCCACCGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATAAGTCCTCCCCAACTAATGAAATCGCCTGCTCCTCCAAATTTTCAGGTACCTTGTCACCATACTTTTTTTTCt                       <  2:19285/138‑1 (MQ=255)
                                                                                                       cAGGCAATTCTTTTGCACTTCTTCCCCACCGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATAAGTCCTCCCCAACTAATGAAATCGCCTGCTCCTCCAAATTTTCAGGTACCTTGTCACCATACTTTTTTTTCTg                      <  2:1188781/139‑1 (MQ=255)
                                                                                                        aGGCAATTCTTTTGCACTTCTTCCCCACCGCTTCTCCGtat                                                                                                                       >  1:1319722/1‑41 (MQ=255)
                                                                                                        aGGCAATTCTTTTGCACTTCTTCCCCACCGCTTCTCCGtat                                                                                                                       <  2:1319722/41‑1 (MQ=255)
                                                                                                        aGGCAATTCTTTTGCACTTCTTCCCCACCGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATAAGTCCTCCCCAACTAATGAAATCGCCTGCTCCTCCAAATTTTCAGGTACCTTGTCACCAt                                   <  1:1189498/125‑1 (MQ=255)
                                                                                                        aGGCAATTCTTTTGCACTTCTTCCCCACCGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATAAGTCCTCCCCAACTAATGAAATCGCCTGCTCCTCCAAATTTTCAGGTACCTTGTCACCAt                                   >  2:1189498/1‑125 (MQ=255)
                                                                                                        aGGCAATTCTTTTGCACTTCTTCCCCACCGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATAAGTCCTCCCCAACTAATGAAATCGCCTGCTCCTCCAAATTTTCAGGTACCTTGTCACCATACTTTTTTTTCTGa                     >  2:1178257/1‑139 (MQ=255)
                                                                                                         ggCAATTCTTTTGCACTTCTTCCCCACCGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATAAGtcctc                                                                                        <  1:73868/71‑1 (MQ=255)
                                                                                                         ggCAATTCTTTTGCACTTCTTCCCCACCGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATAAGtcctc                                                                                        >  2:73868/1‑71 (MQ=255)
                                                                                                         ggCAATTCTTTTGCACTTCTTCCCCACCGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATAAGTCCTCCCCAACTAATGAAATCGCCTGCTCCTCCAAATTTTCAGGTACCTTGTCACCATACTTTTTTTTCTGAg                    >  2:576820/1‑139 (MQ=255)
                                                                                                          gCAATTCTTTTGCACTTCTTCCCCACCGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATAAGTCCTCCCCAACTAATGAAATCGCCTGCTCCTCCAAATTTTCAgg                                                 >  1:879135/1‑109 (MQ=255)
                                                                                                          gCAATTCTTTTGCACTTCTTCCCCACCGCTTCTCCGTATAAACCTTTATCAATGCTTGGTATAAGTCCTCCCCAACTAATGAAATCGCCTGCTCCTCCAAATTTTCAgg                                                 <  2:879135/109‑1 (MQ=255)
                                                                                                            aaTTCTTTTGCACTTCTTCCCCACCGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATAAGTCCTCCCCAACTAATGAAATCGCCTGCTCCTCCAAATTTTCAgg                                                 <  2:349359/107‑1 (MQ=255)
                                                                                                            aaTTCTTTTGCACTTCTTCCCCACCGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATAAGTCCTCCCCAACTAATGAAATCGCCTGCTCCTCCAAATTTTCAgg                                                 >  1:349359/1‑107 (MQ=255)
                                                                                                                 ttttGCACTTCTTCCCCACCGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATAAGTCCTCCCCAACTAATGAAATCGCCTGCTCCTCCAAATTTTCAGGTACCTTGTCACCATACTTTTTTTTCTGAGCATTAATg            <  2:795986/139‑1 (MQ=255)
                                                                                                                    tGCACTTCTTCCCCACCGCTTCTCCGTATAACCCTTTATCAATGCtt                                                                                                     >  1:1305120/1‑47 (MQ=255)
                                                                                                                    tGCACTTCTTCCCCACCGCTTCTCCGTATAACCCTTTATCAATGCtt                                                                                                     <  2:1305120/47‑1 (MQ=255)
                                                                                                                      cACTTCTTCCCCACCGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATAAGTCCTCCCCAACTAATGAAATCGCCTGCTCCTCCAAATTTTCAGGTACCTTGTCACCATACTTTTTTTTCTGAGCATTAATGATAtt       >  1:413455/1‑139 (MQ=255)
                                                                                                                           cttcCCCACCGCTTCTCCGTATGACCCTTTATCAATGCTTGGTATAAGTCCTCCCCAACTAATGAAATCGCCTGCTCCTCCAAATTTTCAGGTACCTTGTCACCATACTTTTTTTTCTGAGCATTAATGATATTTTGAg  >  1:604936/1‑139 (MQ=255)
                                                                                                                           cttcCCCACCGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATAAGTCCTCCCCAACTAATGAAATCGCCTGCTCCTCCAAATTTTCAgg                                                 <  2:260850/92‑1 (MQ=255)
                                                                                                                           cttcCCCACCGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATAAGTCCTCCCCAACTAATGAAATCGCCTGCTCCTCCAAATTTTCAGGTACCTTGTCACCATACTTTTTTTTCTGAGCATTAATGATATTTTGAg  <  2:1224608/139‑1 (MQ=255)
                                                                                                                           cttcCCCACCGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATAAGTCCTCCCCAACTAATGAAATCGCCTGCTCCTCCAAATTTTCAGGTACCTTGTCACCATACTTTTTTTTCTGAGCATTAATGATATTTTGAg  <  1:423977/139‑1 (MQ=255)
                                                                                                                           cttcCCCACCGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATAAGTCCTCCCCAACTAATGAAATCGCCTGCTCCTCCAAATTTTCAGGTACCTTGTCACCATACTTTTTTTTCTGAGCATTAATGATATTTTGAg  >  1:708836/1‑139 (MQ=255)
                                                                                                                           cttcCCCACCGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATAAGTCCTCCCCAACTAATGAAATCGCCTGCTCCTCCAAATTTCCAgg                                                 >  1:260850/1‑92 (MQ=255)
                                                                                                                                    |                                                                                                                                 
TCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAATCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATAAGTCCTCCCCAACTAATGAAATCGCCTGCTCCTCCAAATTTTCAGGTACCTTGTCACCATACTTTTTTTTCTGAGCATTAATGATATTTTGAG  >  NC_000913/2107734‑2107995

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A