Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I2 R1
|
78 |
65.3 |
3016648 |
94.6% |
2853749 |
111.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,256,048 |
G→A |
R202C (CGC→TGC) |
yhaM ← |
putative L‑serine dehydratase alpha chain |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,256,048 | 0 | G | A | 100.0%
| 248.8
/ NA
| 70 | R202C (CGC→TGC) | yhaM | putative L‑serine dehydratase alpha chain |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (33/37); total (33/37) |
ATGAAGAGAGATCTTTCGCCAGCAAACCGCGCTCGCACTGTTTTTCCAGCGTCGCGCCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACC > NC_000913/3255913‑3256174
|
aTGAAGAGAGATCTTTCGCCAGCAAACCGCGCTCGCACTGTTTTTCCAGCGTCGCGCCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGAt < 1:366392/139‑1 (MQ=255)
agagagATCTTTCGCCAGCAAACCGCGCTCGCACTGTTTTTCCAGCGTCGCGCCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGATcgcc > 1:79152/1‑139 (MQ=255)
cTTTCGCCAGCAAACCGCGCTCGCACTGTTTTTCCAGCGTCGCGCCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGATCGCCGCAAACgg < 1:747443/139‑1 (MQ=255)
cTTTCGCCAGCAAACCGCGCTCGCACTGTTTTTCCAGCGTCGCGCCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGATCGCCGCAAACgg < 1:269131/139‑1 (MQ=255)
tttCGCCAGCAAACCGCGCTCGCACTGTTTTTCCAGCGTCGCGCCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGATCGCCGCAAACggg > 1:1458285/1‑139 (MQ=255)
ttCGCCAGCAAACCGCGCTCGCACTGTTTTTCCAGCGTCGCGCCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGATCGCCGCAAACGGGa > 1:557229/1‑139 (MQ=255)
ttCGCCAGCAAACCGCGCTCGCACTGTTTTTCCAGCGTCGCGCCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGATCGCCGCAAACGGGa < 2:557229/139‑1 (MQ=255)
ccAGCAAACCGCGCTCGCACTGTTTTTCCAGCGTCGCGCCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGATCGCCGCAAACGGGACTTc > 2:340523/1‑139 (MQ=255)
aGCAAACCGCGCTCGCACTGTTTTTCCAGCGTCGCGCCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGATcgccg > 1:74811/1‑124 (MQ=255)
aGCAAACCGCGCTCGCACTGTTTTTCCAGCGTCGCGCCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGATcgccg < 2:74811/124‑1 (MQ=255)
aGCAAACCGCGCTCGCACTGTTTTTCCAGCGTCGCGCCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGATCGCCGCAAACGGGACTTCAt > 2:869032/1‑139 (MQ=255)
cAAACCGCGCTCGCACTGTTTTTCCAGCGTCGCGCCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGATCGCCGCAAACGGGACTTCATTg > 1:989503/1‑139 (MQ=255)
aCCGCGCTCGCACTGTTTTTCCAGCGTCGCGCCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGATCGCCGCAAACGGGACTTCATTGACg < 1:121175/139‑1 (MQ=255)
cTCGCACTGTTTTTCCAGCGTCGCGCCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGATCGCCGCAAACGGGACTTCATTGACGAACTTc > 2:1388485/1‑139 (MQ=255)
tCGCACTGTTTTTCCAGCGTCGCGCCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGATCGCCGCAAACGGGACTTCATTGACGAACTTCa < 1:869032/139‑1 (MQ=255)
aCTGTTTTTCCAGCGTCGCGCCAATATGCAGCCCCCACTTGCCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGATCGCAGCAAACGGGACTTCATTGACGAACTTCAGGAt < 1:665130/139‑1 (MQ=255)
aCTGTTTTTCCAGCGTCGCGCCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGAt < 1:1388485/139‑1 (MQ=255)
tttttCCAGCGTCGCGCCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTca > 2:1042931/1‑139 (MQ=255)
ccAGCGTCGCGCCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTcagccag < 1:1477193/139‑1 (MQ=255)
ccAGCGTCGCGCCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTcagccag < 1:1392204/139‑1 (MQ=255)
cAGCGTCGCGCCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGATCGCCGCAAACGGGa < 1:375371/107‑1 (MQ=255)
cAGCGTCGCGCCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGATCGCCGCAAACGGGa > 2:375371/1‑107 (MQ=255)
aGCGTCGCGCCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGcg < 2:424598/139‑1 (MQ=255)
aGCGTCGCGCCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGcg < 2:675257/139‑1 (MQ=255)
aGCGTCGCGCCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGcg < 2:1411466/139‑1 (MQ=255)
aGCGTCGCGCCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGcg < 2:171064/139‑1 (MQ=255)
gCGTCGCGCCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGATcgcc < 1:551812/95‑1 (MQ=255)
gCGTCGCGCCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGATcgcc > 2:551812/1‑95 (MQ=255)
cgcgCCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGATcgcc < 1:566167/91‑1 (MQ=255)
cgcgCCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGATcgcc > 2:566167/1‑91 (MQ=255)
cgcCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCt > 1:1266563/1‑139 (MQ=255)
atGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGc < 2:79152/139‑1 (MQ=255)
aGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCg > 1:395443/1‑139 (MQ=255)
gCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGATCGCCGCAAACGGGACCTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGt < 1:747420/139‑1 (MQ=255)
cccccACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTc > 2:732650/1‑139 (MQ=255)
cccACTTACTGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGATCGCCGCAAACGGGACTTCATTGACGAACTTAAGGATCTCAGACAGCGTCGTTCTGGAAAGAACCGTCAg > 2:1286338/1‑139 (MQ=255)
cccACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGATCGCCGCAAACGGGACTTCATTGACg > 2:1221714/1‑95 (MQ=255)
cccACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGATCGCCGCAAACGGGACTTCATTGACg < 1:1221714/95‑1 (MQ=255)
aCCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGATCGCCGCaaa < 2:647767/72‑1 (MQ=255)
aCCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGATCGCCGCaaa > 1:647767/1‑72 (MQ=255)
ccGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGAct > 1:43678/1‑139 (MQ=255)
gCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGATCGCCGCAAACGGGACTTCATTGACGAAc < 1:822309/88‑1 (MQ=255)
gCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGATCGCCGCAAACGGGACTTCATTGACGAAc > 2:822309/1‑88 (MQ=255)
aaaCCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGTAAAGCACCGTCAGCGGAGACTCTTGCt < 1:1453788/139‑1 (MQ=255)
aaaCCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCt < 2:823273/139‑1 (MQ=255)
ccTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCctc > 2:1287535/1‑139 (MQ=255)
gATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCca < 2:989503/139‑1 (MQ=255)
tAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCcaca > 1:352349/1‑139 (MQ=255)
aaCGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGATCGCCGCAAACGGGACTTCATTGACGAACTTCa > 1:913375/1‑73 (MQ=255)
aaCGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGATCGCCGCAAACGGGACTTCATTGACGAACTTCa < 2:913375/73‑1 (MQ=255)
cGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCacacac > 2:868376/1‑139 (MQ=255)
cGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCacacac > 2:892346/1‑139 (MQ=255)
cacaATTTAGCTTCGCGGAATCGAGAATAAAGCAGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGc < 2:176227/139‑1 (MQ=255)
caATTTAGCTTCGCGGAATCGAGAATAAAGCAGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCct < 2:1458285/139‑1 (MQ=255)
aTTTAGCTTCGCGGAATCGAGAATAAAGCAGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCctgc < 1:868376/139‑1 (MQ=255)
cgcgGAATCGAGAATAAAGCAGATCGCCGCAAACGGGAc > 2:303943/1‑39 (MQ=255)
cgcgGAATCGAGAATAAAGCAGATCGCCGCAAACGGGAc < 1:303943/39‑1 (MQ=255)
gcgGAATCGAGAATAAAGCAGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAaca < 1:732650/139‑1 (MQ=255)
cgGAATCGAGAATAAAGCAGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGcgtcg > 2:491146/1‑72 (MQ=255)
cgGAATCGAGAATAAAGCAGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGcgtcg < 1:491146/72‑1 (MQ=255)
cgGAATCGAGAATAAAGCAGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGaaa > 2:1357425/1‑81 (MQ=255)
cgGAATCGAGAATAAAGCAGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGaaa < 1:1357425/81‑1 (MQ=255)
cgGAATCGAGAATAAAGCAGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAacac > 1:1312675/1‑139 (MQ=255)
cgGAATCGAGAATAAAGCAGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAacac > 1:828026/1‑139 (MQ=255)
cgGAATCGAGAATAAAGCAGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTCGGTAAacac > 2:1030165/1‑139 (MQ=255)
gAATCGAGAATAAAGCAGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAacacca < 1:892346/139‑1 (MQ=255)
agaATAAAGCAGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAacaccacacc > 1:925383/1‑137 (MQ=255)
agaATAAAGCAGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAacaccacacc < 2:925383/137‑1 (MQ=255)
aGCAGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGt < 1:242833/73‑1 (MQ=255)
aGCAGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGt > 2:242833/1‑73 (MQ=255)
|
ATGAAGAGAGATCTTTCGCCAGCAAACCGCGCTCGCACTGTTTTTCCAGCGTCGCGCCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACC > NC_000913/3255913‑3256174
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
