Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F1 I2 R1	78	48.0	2243616	94.8%	2126947	114.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,432,289	G→A	G1081S (GGT→AGT)	stfR →	Rac prophage, putative tail fiber protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,432,289	0	G	A	100.0%	220.7 / NA	63	G1081S (GGT→AGT)	stfR	Rac prophage, putative tail fiber protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (31/32); total (31/32)

GTGCTGGTAGTGCATCAACAAGATTGTCTGTTGTGCATAATCAAAACTATGCAACATCATCTGCTGGCGCACATACCCACTCACTGTCCGGCACTGCTGCAAGCGCAGGTGCACACGCGCATACTGTCGGTATTGGTGCTCATACGCACTCCGTTGCGATTGGTTCACATGGACACACCATCACCGTTAACGCTGCTGGTAACGCGGAAAACACCGTCAAAAACATCGCATTTAACTATATTGTGAGGCTTGCA  >  NC_000913/1432155‑1432408

gTGCTGGTAGTGCATCAACAAGATTGTCTGTTGTGCATAATCAAAACTATGCAACATCATCTGCTGGCGCACATACCCACTCACTGTCCGGCACTGCTGCAAGCGCAGGTGCACACGCGCATACTGTCGGTATTAGTGc                                                                                                                     >  1:568563/1‑139 (MQ=255)

 tGCTGGTAGTGCATCAACAAGATTGTCTGTTGTGCATAATCAAAACTATGCAACATCATCTGCTGGCGCACATACCCACTCACTGTCCGGCACTGCTGCAAGCGCAGGTGCACACGCGCATACTGTCGGTATTAGTGCt                                                                                                                    <  1:469855/139‑1 (MQ=255)

         gTGCATCAACAAGATTGTCTGTTGTGCATAATCAAAACTATGCAACATCATCTGCTGGCGCACATACCCACTCACTGTCCGGCACTGCTGCAAGCGCAGGTGCACACGCGCATACTGTCGGTATTAGTGCTCATACGCa                                                                                                            <  2:568563/139‑1 (MQ=255)

           gCATCAACAAGATTGTCTGTTGTGCATAATCAAAACTATGCAACATCATCTGCTGGCGCACATACCCACTCACTGTCCGGCACTGCTGCAAGCGCAGGTGCACACGCGCATACTGTCGGTATTAGTGCTCATACGCACt                                                                                                          >  2:141993/1‑139 (MQ=255)

                    aGATTGTCTGTTGTGCATAATCAAAACTATGCAACATCATCTGCTGGCGCACATACCCACTCACTGTCCGGCACTGCTGCAAGCGCAGGTGCACACGCGCATACTGTCGGTATTAGTGCTCATACGCACTCCGTTGCGa                                                                                                 >  2:532674/1‑139 (MQ=255)

                     gATTGTCTGTTGTGCATAATCAAAACTATGCAACATCATCTGCTGGCGCACATACCCACTCACTGTCCGGCACTGCTGCAAGCGCAGGTGCACACGCGCATACCGTCGGTATTAGTGCTCATACGCACTCCGTTGCGAt                                                                                                <  2:826627/139‑1 (MQ=255)

                        tGTCTGTTGTGCATAATCAAAACTATGCAACATCATCTGCTGGCGCACATACCCACTCACTGTCCGGCACTGCTGCAAGCGCAGGTGCACACGCGCATACTGTCGGTATTAGTGCTCATACGCACTCCGTTGCGATTgg                                                                                             <  2:135892/139‑1 (MQ=255)

                         gTCTGTTGTGCATAATCAAAACTATGCAACATCATCTGCTGGCGCACATACCCACTCACTGTCCGGCACTGCTGCAAGCGCAGGTGCACACGCGCATACTGTCGGTATTAGTGCTCATACGCACTCCGTTGCGATTGGt                                                                                            >  2:123951/1‑139 (MQ=255)

                               tgtgCATAATCAAAACTATGCAACATCATCTGCTGGCGCACATACCCACTCACTGTCCGGCACTGCTGCAAGCGCAGGTGCACACGCGCATACTGTCGGTATTAGTGCt                                                                                                                    >  1:756349/1‑109 (MQ=255)

                               tgtgCATAATCAAAACTATGCAACATCATCTGCTGGCGCACATACCCACTCACTGTACGGCACTGCTGCAAGCGCAGGTGCACACGCGCATACTGTCGGTATTAGTGCt                                                                                                                    <  2:756349/109‑1 (MQ=255)

                                gtgCATAATCAAAACTATGCAACATCATCTGCTGGCGCACATACCCACTCACTGTCCGGCACTGCTGCAAGCGCAGGTGCACACGCGCATACTGTCGGTATTAGTGCTCATACGCACTCCGTTGCGATTGGTTCACATg                                                                                     >  1:399286/1‑139 (MQ=255)

                                      aaTCAAAACTATGCAACATCATCTGCTGGCGCACATACCCACTCACTGTCCGGCACTGCTGCAAGCGCAGGTGCACACGCGCATACTGTCGGTATTAGTGCTCATACGCACTCCGTTGCGATTGGTTCACATGGacaca                                                                               >  1:94103/1‑139 (MQ=255)

                                        tCAAAACTATGCAACATCATCTGCTGGCGCACATACCCACTCACTGTCCGGCACTGCTGCAAGCGCAGGTGCACACGCGCATACTGTCGGTATTAGTGCTCATACGCACTCCGTTGCGATTGGTTCACATGGACACAcc                                                                             >  2:564487/1‑139 (MQ=255)

                                          aaaaCTATGCAACATCATCTGCTGGCGCACATACCCACTCACTGTCCGGCACTGCTGCAAGCGCAGGTGCACACGCGCATACTGTCGGTATTAGTGCTCATACGCACTCCGTTGCGATTGGTTCACATGGACACACCAt                                                                           <  2:94103/139‑1 (MQ=255)

                                           aaaCTATGCAACATCATCTGCTGGCGCACATACCCACTCACTGTCCGGCACTGCTGCAAGCGCAGGTGCACACGCGCATACTGTCGGTATTAGTGCTCATACGCACTCCGTTGCGATTGGTTCACATGGACACACCATc                                                                          >  1:129699/1‑139 (MQ=255)

                                                aTGCAACATCATCTGCTGGCGCACATACCCACTCACTGTCCGGCACTGCTGCAAGCGCAGGTGCACACGCGCATACTGTCGGTATTAGTGCTCATACGCACTCCGTTGCGATTGGTTCACATGGACACACCATCACCGt                                                                     >  2:376895/1‑139 (MQ=255)

                                                 tGCAACATCATCTGCTGGCGCACATACCCACTCACTGTCCGGCACTGCTGCAAGCGCAGGTGCACACGCGCATACTGTCGGTATTAGTGCTCATACGCACTCCGTTGCGATTGGtt                                                                                           <  1:981071/116‑1 (MQ=255)

                                                 tGCAACATCATCTGCTGGCGCACATACCCACTCACTGTCCGGCACTGCTGCAAGCGCAGGTGCACACGCGCATACTGTCGGTATTAGTGCTCATACGCACTCCGTTGCGATTGGtt                                                                                           >  2:981071/1‑116 (MQ=255)

                                                  gCAACATCATCTGCTGGCGCACATACCCACTCACTGTCCGGCACTGCTGCAAGCGCAGGTGCACACGCGCATACTGTCGGTATTAGTg                                                                                                                      >  1:175709/1‑88 (MQ=255)

                                                  gCAACATCATCTGCTGGCGCACATACCCACTCACTGTCCGGCACTGCTGCAAGCGCAGGTGCACACGCGCATACTGTCGGTATTAGTg                                                                                                                      <  2:175709/88‑1 (MQ=255)

                                                  gCAACATCATCTGCTGGCGCACATACCCACTCACTGTCCGGCACTGCTGCAAGCGCAGGTGCACACGCGCATACTGTCGGTATTAGTGCTCATACGCACTCCGTTGCGATTGGTTCACATGGACACACCATCACCGTTa                                                                   >  2:898645/1‑139 (MQ=255)

                                                   cAACATCATCTGCTGGCGCACATACCCACTCACTGTCCGGCACTGCTGCAAGCGCAGGTGCACACGCGCATACTGTCGGTATTAGTGCTCATACGCACTCCGTTGCGATTGGTTCACATGGACACACCATCACCGTTaa                                                                  <  2:226202/139‑1 (MQ=255)

                                                     aCATCATCTGCTGGCGCACATACCCACTCACTGTCCGGCACTGCTGCAAGCGCAGGTGCACACGCGCATACTGTCGGTATTAGTGCTCATACGCACTCCGTTGCGATTGGTTCACATGGACACACCATCACCGTTAACg                                                                <  1:564487/139‑1 (MQ=255)

                                                           tcTGCTGGCGCACATACCCACTCACTGTCCGGCACTGCTGCAAGCGCAGGTGCACACGCGCATACTGTCGGTATTAGTGCTCATACGCACTCCGTTGCGATTgg                                                                                             <  2:194056/104‑1 (MQ=255)

                                                           tcTGCTGGCGCACATACCCACTCACTGTCCGGCACTGCTGCAAGCGCAGGTGCACACGCGCATACTGTCGGTATTAGTGCTCATACGCACTCCGTTGCGATTgg                                                                                             >  1:194056/1‑104 (MQ=255)

                                                           tcTGCTGGCGCACATACCCACTCACTGTCCGGCACTGCTGCAAGCGCAGGTGCACACGCGCATACTGTCGGTATTAGTGCTCATACGCACTCCGTTGCGATTGGTTCACATGGACACACCATCACCGTTAACGctgctg                                                          >  2:908756/1‑139 (MQ=255)

                                                              gctgGCGCACATACCCACTCACTGTCCGGCACTGCTGCAAGCGCAGGTGCACACGCGCATACTGTCGGTATTAGTGCTCATACGCACTCCGTTGCGATTGGTTCACATGGACACACCATCACCGTTAACGCTGCTGGTa                                                       <  1:318901/139‑1 (MQ=255)

                                                                tgGCGCACATACCCACTCACTGTCCGGCACTGCTGCAAGCGCAGGTGCACACGCGCATACTGTCGGTATTAGTGCTCATACGCACTCCGTTGCGATTGGTTCACATGGACACACCATCACCGTTAACGCTGCTGGTAAc                                                     >  2:670850/1‑139 (MQ=255)

                                                                 ggCGCACATACCCACTCACTGTCCGGCACTGCTGCAAGCGCAGGTGCACACGCGCATACTGTCGGTATTAGTGCTCATACGCACTCCGTTGCGATTGGTTCACATGGACACACCATCACCGTTAACGGTGCTGGTAAcg                                                    >  2:726500/1‑139 (MQ=255)

                                                                   cgcACATACCCACTCACTGTCCGGCACTGCTGCAAGCGCAGGTGCACACGCGCATACTGTCGGTATTAGTGCTCATACGCACTCCGTTGCGATTGGTTCACATg                                                                                     <  1:437427/104‑1 (MQ=255)

                                                                   cgcACATACCCACTCACTGTCCGGCACTGCTGCAAGCGCAGGTGCACACGCGCATACTGTCGGTATTAGTGCTCATACGCACTCCGTTGCGATTGGTTCACATg                                                                                     >  2:437427/1‑104 (MQ=255)

                                                                         tACCCACTCACTGTCCGGCACTGCTGCAAGCGCAGGTGCACACGCGCATACTGTCGGTATTAGTGCTCATACGCACTCCGTTGCGATTGGTTCACATGGACACACCATCACCGTTAACGCTGCTGGTAACGCGGAAAac                                            <  1:958566/139‑1 (MQ=255)

                                                                               ctcactGTCCGGCACTGCTGCAAGCGCAGGTGCACACGCGCATACTGTCGGTATTAGTGCTCATACGCACTCCGTTGCGATTGGTTCACATGGACACACCATCACCGTTAACGCTGCTGGTAACGCGGAAAACACCGTc                                      >  1:890824/1‑139 (MQ=255)

                                                                                 cactGTCCGGCACTGCTGCAAGCGCAGGTGCATACGCGCATACTGTCGGTATTAGTGCTCATACGCACTCCGTTGCGATTGGTTCACATGGACACACCATCACCGTTAACGCTGCTGGTAACGCGGAAAACACCGTCaa                                    >  1:664646/1‑139 (MQ=255)

                                                                                    tGTCCGGCACTGCTGCAAGCGCAGGTGCACACGCGCATACTGTCGGTATTAGTGCTCATACGCACTCCGTTGCGATTGGTTCACATGGACACACCATCAc                                                                        <  1:162022/100‑1 (MQ=255)

                                                                                    tGTCCGGCACTGCTGCAAGCGCAGGTGCACACGCGCATACTGTCGGTATTAGTGCTCATACGCACTCCGTTGCGATTGGTTCACATGGACACACCATCAc                                                                        >  2:162022/1‑100 (MQ=255)

                                                                                    tGTCCGGCACTGCTGCAAGCGCAGGTGCACACGCGCATACTGTCGGTATTAGTGCTCATACGCACTCCGTTGCGATTGGTTCACATGGACACACCATCACCGTTAACGCTGCTGGTAAc                                                     >  1:196148/1‑119 (MQ=255)

                                                                                    tGTCCGGCACTGCTGCAAGCGCAGGTGCACACGCGCATACTGTCGGTATTAGTGCTCATACGCACTCCGTTGCGATTGGTTCACATGGACACACCATCACCGTTAACGCTGCTGGTAAc                                                     <  2:196148/119‑1 (MQ=255)

                                                                                    tGTCCGGCACTGCTGCAAGCGCAGGTGCACACGCGCATACTGTCGGTATTAGTGCTCATACGCACTCCGTTGCGATTGGTTCACATGGACACACCATCACCGTTAACGCTGCTGGTAACGCGGAAAACACCGTCaaaaa                                 >  2:172627/1‑139 (MQ=255)

                                                                                    tGTCCGGCACTGCTGCAAGCGCAGGTGCACACGCGCATACTGTCGGTATGAGTGCTCATACACACTCCGTTGCGATTGGTTCACATGGACACACCATCACCGTTAACGCAGCTGGTAACGCGGAAAACACCGTCaaaaa                                 >  1:723424/1‑139 (MQ=255)

                                                                                      tCCGGCACTGCTGCAAGCGCAGGTGCACACGCGCATACTGTCGGTATTAGTGCTCATACGCACTCCGTTGCGATTGGTTCACATGGACACACCATCACCGTTAACGCTGCTGGTAACGCGGAAAACACCGTCAAAAACa                               >  1:26343/1‑139 (MQ=255)

                                                                                      tCCGGCACTGCTGCAAGCGCAGGTGCACACGCGCATACTGTCGGTATTAGTGCTCATACGCACTCCGTTGCGATTGGTTCACATGGACACACCATCACCGTTAACGCTGCTGGTAACGCGGAAAACACCGTCAAAAACa                               <  1:532674/139‑1 (MQ=255)

                                                                                      tCCGGCACTGCTGCAAGCGCAGGTGCACACGCGCATACTGTCGGTATTAGTGCTCATACGCACTCCGTTGCGATTGGTTCACATGGACACACCATCACCGTTAACGCTGCTGGTAACGCGGAAAACACCGTCAAAAACa                               >  1:1046822/1‑139 (MQ=255)

                                                                                        cGGCACTGCTGCAAGCGCAGGTGCACACGCGCATACTGTCGGTATTAGTGCTCATACGCACTCCGTTGCGATTGGTTCACATGGACACACCATCACCGTTAACGCTGCTGGTAACGCGGAAAACACCGTCAAAAACATc                             >  2:335788/1‑139 (MQ=255)

                                                                                         ggCACTGCTGCAAGCGCAGGTGCACACGCGCATACTGTCGGTATTAGTGCTCATACGCACTCCGTTGCGATTGGTTAACATGGACACACCATCACCGTTAACGCTGCTGGTAACGCGGAAAACACCGTCAAAAACATCg                            <  2:664646/139‑1 (MQ=255)

                                                                                          gCACTGCTGCAAGCGCAGGTGCACACGCGCATACTGTCGGTATTAGTGCTCATACGCACTCCGTTGCGATTGGTTCACATGGACACACCATCACCGTTAACGCTGCTGGTAACG‑GGAAAACACCGTCAAAAACATCGCa                          <  1:726500/139‑1 (MQ=255)

                                                                                           cACTGCTGCAAGCGCAGGTGCACACGCGCATACTGTCGGTATTAGTGCTCATACGCACTCCGTTGCGATTGGTTCACATGGACACACCATCACCGTTAACGCTGCTGGTAACGCGGAAAACACCGTCAAAAACATCGCa                          <  2:26343/139‑1 (MQ=255)

                                                                                           cACTGCTGCAAGCGCAGGTGCACACGCGCATACTGTCGGTATTAGTGCTCATACGCACTCCGTTGCGATTGGTTCACATGGACACACCATCACCGTTAACGCTGCTGGTAACGCGGAAAACACCGTCAAAAACATCGCa                          <  2:129699/139‑1 (MQ=255)

                                                                                             ctgctgCAAGCGCAGGTGCACACGCGCATACTGTCGGTATTAGTGCTCATACGCACTCCGTTGCGATTGGTTCACATGGACACACCATCACCGTTAACGCTGCTGGTAACGCGGAAAACACCGTCAAAAACATCGCAtt                        <  1:172627/139‑1 (MQ=255)

                                                                                             ctgctgCAAGCGCAGGTGCACACGCGCATACTGTCGGTATTAGTGCTCATACGCACTCCGTTGCGATTGGTTCACATGGACACACCATCACCGTTAACGCTGCTGGTAACGCGGAAAACACCGTCAAAAACATCGCAtt                        <  1:141993/139‑1 (MQ=255)

                                                                                             ctgctgCAAGCGCAGGTGCACACGCGCATACTGTCGGTATTAGTGCTCATACGCACTCCGTTGCGATTGGTTCACATGGACACACCATCACCGTTAACGCTGCTGGTAACGCGGAAAACACCGTCAAAAACATCGCAtt                        <  2:844416/139‑1 (MQ=255)

                                                                                                ctgcAAGCGCAGGTGCACACGCGCATACTGTCGGTATTAGTGCTCATACGCACTCCGTTGCGATTGGTTCACATGGACACACCATCACCGTTAACGCTGCTGGTAACGCGGAAAACACCGTCAAAAACATCGCATTTaa                     >  1:354397/1‑139 (MQ=255)

                                                                                                  gcAAGCGCAGGTGCACACGCGCATACTGTCGGTATTAGTGCTCATACGCACTCCGTTGCGATTGGTTCACATGGACACACCATCACCGTTAACGCTGCTGGTAACGCGGAAAACACCGTCAAAAACATCGCATTTAACt                   <  2:491692/139‑1 (MQ=255)

                                                                                                     aGCGCAGGTGCACACGCGCATACTGTCGGTATTAGTGCTCATACGCACTCCGTTGCGATTGGTTCACATGGacaca                                                                               <  2:382269/76‑1 (MQ=255)

                                                                                                     aGCGCAGGTGCACACGCGCATACTGTCGGTATTAGTGCTCATACGCACTCCGTTGCGATTGGTTCACATGGacaca                                                                               >  1:382269/1‑76 (MQ=255)

                                                                                                     aGCGCAGGTGCACACGCGCATACTGTCGGTATTAGTGCTCATACGCACTCCGTTGCGATTGGTTCACATGGacaca                                                                               <  2:920500/76‑1 (MQ=255)

                                                                                                     aGCGCAGGTGCACACGCGCATACTGTCGGTATTAGTGCTCATACGCACTCCGTTGCGATTGGTTCACATGGacaca                                                                               >  1:920500/1‑76 (MQ=255)

                                                                                                         cAGGTGCACACGCGCATACTGTCGGTATTAGTGCTCATACGCACTCCGTTGCGATTGGTTCACATGGACACACCATCACCGTTAACGCTGCTGGTAACGCGGAAAACACCGTCAAAAACATCGCATTTAACTATATtgt            <  1:123951/139‑1 (MQ=255)

                                                                                                         cAGGTGCACACGCGCATACTGTCGGTATTAGTGCTCATACGCACTCCGTTGCGATTGGTTCACATGGACACACCATCACCGTTAACGCTGCTGGTAACGCGGAAAACACCGTCAAAAACATCGCATTTAACTATATtgt            <  2:1046822/139‑1 (MQ=255)

                                                                                                          aGGTGCACACGCGCATACTGTCGGTATTAGTGCTCATACGCACTCCGTTGCGATTGGTTCACATGGACACACCATCACCGTTAACGCTGCTGGTAACGCGGAAAACACCGTCAAAAACATCGCATTTAACTATATtgtg           >  1:545148/1‑139 (MQ=255)

                                                                                                             tGCACACGCGCATACTGTCGGTATTAGTGCTCATACGCACTCCGTTGCGATTGGTTCACATGGACACACCATCACCGTTAACGCTGCTGGTAACGCGGAAAACACCGTCAAAAACATCGCATTTAACTATATTGTGAgg        <  2:1111061/139‑1 (MQ=38)

                                                                                                              gCACACGCGCATACTGTCGGTATTAGTGCTCATACGCACTCCGTTGCGATTGGTTCACATGGACACACCATCACCGTTAACGCTGCTGGTAACGCGGAAAACACCGTCAAAAACATCGCATTTAACTATATTGTGAGGc       <  2:890824/139‑1 (MQ=37)

                                                                                                                   cgcgCATACTGTCGGTATTAGTGCTCATACGCACTCCGTTGCGATTGGTTCACATGGACACACCATCACCGTTAACGCTGCTGGTAACGCGGAAAACACCGTCAAAAACATCGCATTTAACTATATTGTGAGGCTTGCa  <  2:1052404/139‑1 (MQ=18)

GTGCTGGTAGTGCATCAACAAGATTGTCTGTTGTGCATAATCAAAACTATGCAACATCATCTGCTGGCGCACATACCCACTCACTGTCCGGCACTGCTGCAAGCGCAGGTGCACACGCGCATACTGTCGGTATTGGTGCTCATACGCACTCCGTTGCGATTGGTTCACATGGACACACCATCACCGTTAACGCTGCTGGTAACGCGGAAAACACCGTCAAAAACATCGCATTTAACTATATTGTGAGGCTTGCA  >  NC_000913/1432155‑1432408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A