Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F1 I2 R1	78	48.0	2243616	94.8%	2126947	114.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,141,143	A→G	L157P (CTG→CCG)	asmA ←	suppressor of OmpF assembly mutants, putative outer membrane protein assembly factor, inner membrane‑anchored periplasmic protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,141,143	0	A	G	100.0%	148.2 / NA	40	L157P (CTG→CCG)	asmA	suppressor of OmpF assembly mutants, putative outer membrane protein assembly factor, inner membrane‑anchored periplasmic protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (19/21); total (19/21)

TATCGTGAGATCGCGCTGATCGCGATTAACCCGCCCGGAGAACTCAAATGAACCACGATGTTGGGGATCTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCAGCACGCTGTCCGCCACCTTAAGACTGGATATATCAAACGACCATCCGCGATCGTCTGACAGATCCGGCAAGGTATTGTCGCGCGGTGCAACCGGAGCGTCTTCACTGCGCACCGCT  >  NC_000913/2141010‑2141259

tatCGTGAGATCGCGCTGATCGCGATTAAGCTGCCCGGAGAACTCAAATGAACCACGCTGTTGGGGATCTTGTTCCATTTGCAGGCGAATATTGCGTATTGCCACCTGCTCGTCATCTTCATCCTGGAACCcacgcacg                                                                                                                 >  1:912874/1‑139 (MQ=255)

tatCGTGAGATCGCGCTGATCGCGATTAACCCGCCCGGAGAACTCAAATGAACCACGATGTTGGGGATCTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACg                                                                                                                 <  1:804936/139‑1 (MQ=255)

tatCGTGAGATCGCGCTGATCGCGATTAACCCGCCCGGAGAACTCAAATGAACCACGATGTTGGGGATCTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACg                                                                                                                 <  2:30041/139‑1 (MQ=255)

   cGTGAGATCGCGCTGATCGCGATTAACCCGCCCGGAGAACTCAAATGAACCACGATGTTGGGGATCTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTg                                                                                                              >  2:1003669/1‑139 (MQ=255)

        gaTCGCGCTGATCGCGATTAACCCGCCCGGAGAACTCAAATGAACCACGATGTTGGGGATCTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGc                                                                                                         <  1:1003669/139‑1 (MQ=255)

           cgcgCTGATCGCGATTAACCCGCCCGGAGAACTCAAATGAACCACGATGTTGGGGATCTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCAc                                                                                                      >  2:247908/1‑139 (MQ=255)

                   tCGCGATTAACCCGCCCGGAGAACTCAAATGAACCACGATGTTGGGGATCTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCACCTTAAGAc                                                                                              >  1:853367/1‑139 (MQ=255)

                   tCGCGATTAACCCGCCCGGAGAACTCAAATGAACCACGATGTTGGGGATCTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCACCTTAAGAc                                                                                              <  1:442207/139‑1 (MQ=255)

                    cgcgATTAACCCGCCCGGAGAACTCAAATGAACCACGATGTTGGGGATCTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGc                                                                                                         <  1:94429/127‑1 (MQ=255)

                    cgcgATTAACCCGCCCGGAGAACTCAAATGAACCACGATGTTGGGGATCTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGc                                                                                                         >  2:94429/1‑127 (MQ=255)

                       gATTAACCCGCCCGGAGAACTCAAATGAACCACGATGTTGGGGATCTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCACCTTAAGa                                                                                               <  2:1099946/134‑1 (MQ=255)

                       gATTAACCCGCCCGGAGAACTCAAATGAACCACGATGTTGGGGATCTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCACCTTAAGa                                                                                               >  1:1099946/1‑134 (MQ=255)

                            aCCCGCCCGGAGAACTCAAATGAACCACGATGTTGGGGATCTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCACCTTAAGACTGGatatat                                                                                     >  1:532202/1‑139 (MQ=255)

                                 cccgGAGAACTCAAATGAACCACGATGTTGGGGATCTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCACCTTAAGa                                                                                               >  1:606484/1‑124 (MQ=255)

                                 cccgGAGAACTCAAATGAACCACGATGTTGGGGATCTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCACCTTAAGa                                                                                               <  2:606484/124‑1 (MQ=255)

                                   cgGAGAACTCAAATGAACCACGATGTTGGGGATCTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCACCTTAAGACTGGATATATCAAACGa                                                                              <  1:247908/139‑1 (MQ=255)

                                   cgGAGAACTCAAATGAACCACGATGTTGGGGATCTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCACCTTAAGACTGGATATATCAAACGa                                                                              <  2:630192/139‑1 (MQ=255)

                                             aaaTGAACCACGATGTTGGGGATCTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCACCTTAAGACTGGATATATCAAACGACCATCCGCGa                                                                    <  1:404284/139‑1 (MQ=255)

                                                  aaCCACGATGTTGGGGATCTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCACCTTAAGACTGGATATATCAAACGACCATCCGCGATCGTc                                                               >  2:950281/1‑139 (MQ=255)

                                                       cGATGTTGGGGATCTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCACCTTAAGa                                                                                               <  2:1033728/102‑1 (MQ=255)

                                                       cGATGTTGGGGATCTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCACCTTAAGa                                                                                               >  1:1033728/1‑102 (MQ=255)

                                                              ggggATCTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCACCTTAAGACTGGATATATCAAACGACCATCCGCGATCGTCTGACAGATCCgg                                                   <  2:853367/139‑1 (MQ=255)

                                                              ggggATCTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCACCTTAAGACTGGATATATCAAACGACCATCCGCGATCGTCTGACAGATCCgg                                                   >  2:612629/1‑139 (MQ=255)

                                                              ggggATCTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCACCTTAAGACTGGATATATCAAACGACCATCCGCGATCGTCTGACAGATCCgg                                                   <  1:410808/139‑1 (MQ=255)

                                                                      tGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCACCTTAAGACTGGATATATCAAACGACCATCCGCGATCGTCTGACAGATCCgg                                                   <  2:200594/131‑1 (MQ=255)

                                                                      tGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCACCTTAAGACTGGATATATCAAACGACCATCCGCGATCGTCTGACAGATCCgg                                                   >  1:200594/1‑131 (MQ=255)

                                                                      tGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCACCTTAAGACTGGATATATCAAACGACCATCCGCGATCGTCTGACAGATCCGGCAAGGTAt                                           >  2:116378/1‑139 (MQ=255)

                                                                      tGTTCCATTTGCAGGCGAATATTCCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCACCTTAAGACTGGATATATCAAACGACCATCCGCGATCGTCTGACAGATCCGGCAAGGTAt                                           >  1:672233/1‑139 (MQ=255)

                                                                           cATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCACCTTAAGACTGGATATATCAAACGACCATCCGCGATCGTCTGACAGATCCGGCAAGGTATTGTcg                                      <  1:995831/139‑1 (MQ=255)

                                                                             tttGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCACCTTAAGACTGGATATATCAAACg                                                                               >  1:76886/1‑96 (MQ=255)

                                                                             tttGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCACCTTAAGACTGGATATATCAAACg                                                                               <  2:76886/96‑1 (MQ=255)

                                                                             tttGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCACCTTAAGACTGGATATATCAAACGACCATCCGCGATCGTCTGACAGATCCGGCAAGGTATTGTcgcg                                    >  2:554762/1‑139 (MQ=255)

                                                                             tttGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCACCTTAAGACTGGATATATCAAACGACCATCCGCGATCGTCTGACAGATCCGGCAAGGTATTGTcgcg                                    <  1:612629/139‑1 (MQ=255)

                                                                                         tatTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCACCTTAAGACTGGATATATCAAACGACCATCCGCGATCGTCTGACAGATCCGGCAAGGTATTGTCGCGCGGTGCAACCgg                        <  2:579078/139‑1 (MQ=255)

                                                                                                   tGTCACCTGCTCATCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCACCTTAAGACTGGATATATCAAACGACCATCCGCGATCGTCTGACAGATCCGGCAAGGTATTTTCGCGCGGTGCAACCGGAGCGTCTTCa              <  1:667618/139‑1 (MQ=255)

                                                                                                      cACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCACCTTAAGACTGGATATATCAAACGACCATCCGCGATCGTCTGACAGATCCGGCAAGGTATTGTCGCGCGGTGCAACCGGAGCGTCTTCACTg           <  2:455504/139‑1 (MQ=255)

                                                                                                        ccTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCACCTTAAGACTGGATATATCAAACGACCATCCGCGATCGTCTGACAGATCCGGCAAGGTATTGTCGCGCGGTGCAACCGGAGCGTCTTCACTgcg         >  2:649001/1‑139 (MQ=255)

                                                                                                            cTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCACCTTAAGACTGGATATATCAAACGACCATCCGCGATCGTCTGACAGATCCGGCAAGGTATTGTCGCGCGGTGCAACCGGAGCGTCTTCACTGCGCAcc     >  2:584206/1‑139 (MQ=255)

                                                                                                               gTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCACCTTAAGACTGGATATATCAAACGACCATCCGCGATCGTCTGACAGATCCGGCAAGGTATTGTCGCGCGGTGCAACCGGAGCGTCTTCACTGCGCACCGCt  >  2:673055/1‑139 (MQ=255)

                                                                                                                     ttCATGCTGGAACACCGGCACGCTGTCCGCCACCTTAAGACTg                                                                                            >  2:119697/1‑43 (MQ=255)

                                                                                                                     ttCATGCTGGAACACCGGCACGCTGTACGCCACCTTAAGACTg                                                                                            <  1:119697/43‑1 (MQ=255)

TATCGTGAGATCGCGCTGATCGCGATTAACCCGCCCGGAGAACTCAAATGAACCACGATGTTGGGGATCTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCAGCACGCTGTCCGCCACCTTAAGACTGGATATATCAAACGACCATCCGCGATCGTCTGACAGATCCGGCAAGGTATTGTCGCGCGGTGCAACCGGAGCGTCTTCACTGCGCACCGCT  >  NC_000913/2141010‑2141259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A