Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F1 I1 R1
|
82 |
28.6 |
1225488 |
97.4% |
1193625 |
116.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,562,984 |
G→A |
G154D (GGC→GAC) |
yffP → |
CPZ‑55 prophage, uncharacterized protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,562,984 | 0 | G | A | 100.0%
| 95.2
/ NA
| 29 | G154D (GGC→GAC) | yffP | CPZ‑55 prophage, uncharacterized protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (13/16); total (13/16) |
AGCAAGGTGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTACGACATGGAACGGCAAATCAACGCAAACCTGCCGGTTGGCGGCCTTCCCAACGTTGACACAGAGCGAACTGGCAAACTGTTCCAACGCGATGACTTTTCTGAGGATAGCCATGCCAACGATCCGAAGTTGGTTGGTGATGATTACGTCCCCCAGGCAC > NC_000913/2562860‑2563100
|
aGCAAGGTGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTACGACATGGAACGGCAAATCAACGCAAACCTGCCGGTTGGCGACCTTCCCAACGTTg > 1:578052/1‑139 (MQ=255)
aaGGTGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTACGACATGGAACGGCAAATCAACGCAAACCTGCCGGTTGGCGACCTTCCCAACGTTGaca < 2:492224/139‑1 (MQ=255)
ggTGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTACGACATGGAACGGCAAATCAACGCAAACCTGCCGGTTGGCGACCTTCCCAACGTTGaca < 1:221968/137‑1 (MQ=255)
ggTGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTACGACATGGAACGGCAAATCAACGCAAACCTGCCGGTTGGCGACCTTCCCAACGTTGaca > 2:221968/1‑137 (MQ=255)
tGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTACGACATGGAACGGCAAATCAACGCAAACCTGCCGGTTGGCGACCTTCCCAACGTTGACACaga > 2:399100/1‑139 (MQ=255)
gggTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTACGACATGGAACGGCAAATCAACGCAAACCTGCCGGTTGGCGACCTTccc < 1:263685/116‑1 (MQ=255)
gggTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTACGACATGGAACGGCAAATCAACGCAAACCTGCCGGTTGGCGACCTTccc > 2:263685/1‑116 (MQ=255)
cTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTACGACATGGAACGGCAAATCAACGCAAACCTGCCAGTTGGCGACCTTCCCAACGTTGACACAGAGCGAACTGGCAAACTGTTCCAACGCGATGACttt > 1:391802/1‑139 (MQ=255)
cACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTACGACATGGAACGGCAAATCAACGCAAACCTGCCGGTTGGCGACCTTCCCAACGTTGACACAGAGCGAACTGGCAAACTGTTCCAACGCGATGACTTTTCt < 2:391802/139‑1 (MQ=255)
aaCGGTTTCAATACTTCCAAACGATGTACGACATGGAACGGCAAATCAACGCAAACCTGCCGGTTGGCGACCTTCCCAACGTTGACACAGAGCGAACTGGCAAACTGTTCCAACGCGATGACTTTTCTGAGGATAGCCa < 1:556812/139‑1 (MQ=255)
tACTTCCAAACGATGTACGACATGGAACGGCAAATCAACGCAAACCTGCCGGTTGGCGACCTTCCCAACGTTGACACAGAGCGAACTGGCAAACTGTTCCAACGCGATGACTTTTCTGAGGATAGCCATGCCAACGATc < 1:85511/139‑1 (MQ=255)
ttCCAAACGATGTACGACATGGAACGGCAAATCAACGCAAACCTGCCGGTTGGCGACCTTCCCAACGTTGACACAGAGCGAACTGGCAAACTGTTCCAACGCGATGACTTTTCTGAGGATAGCCATGCCAACGATCCGa > 2:402982/1‑139 (MQ=255)
ttCCAAACGATGTACGACATGGAACGGCAAATCAACGCAAACCTGCCGGTTGGCGACCTTCCCAACGTTGACACAGAGCGAACTGGCAAACTGTTCCAACGCGATGACTTTTCTGAGGATAGCCATGCCAACGATCCGa > 2:226434/1‑139 (MQ=255)
cGATGTACGACATGGAACGGCAAATCAACGCAAACCTGCCGGTTGGCGACCTTCCCAACGTTGACACAGAGCGAACTGGCAAACTGTTCCAACGCGATGACTTTTCTGAGGATAGCCATGCCAACGATCCGAAgttggt < 2:14227/139‑1 (MQ=255)
tGTACGACATGGAACGGCAAATCAACGCAAACCTGCCGGTTGGCGACCTTCCCAACGTTGACACAGAGCGAACTGGCAAACTGTTCCAACGCGATGACTTTTCTGAGGATAGCCATGCCAACGATCCGAAGttggttgg > 2:236468/1‑139 (MQ=255)
ggAACGGCAAATCAACGCAAACCTGCCGGTTGGCGACCTTccc > 2:389418/1‑43 (MQ=255)
ggAACGGCAAATCAACGCAAACCTGCCGGTTGGCGACCTTccc < 1:389418/43‑1 (MQ=255)
ggAACGGCAAATCAACGCAAACCTGCCGGTTGGCGACCTTCCCAACGTTGACACAGAGCGAACTGGCAAACTGTTCCAACGCGATGACTTTTCTGAGGATAGCCATGCCAACGATCCGAAGTTGGTTGGTGATGATTAc > 2:268556/1‑139 (MQ=255)
ggAACGGCAAATCAACGCAAACCTGCCGGTTGGCGACCTTCCCAACGTTGACACAGAGCGAACTGGCAAACTGTTCCAACGCGATGACTTTTCTGAGGATAGCCATGCCAACGATCCGAAGTTGGTTGGTGATGATTAc > 2:54652/1‑139 (MQ=255)
ggAACGGCAAATCAACGCAAACCTGCCGGTTGGCGACCTTCCCAACGTTGACACAGAGCGAACTGGCAAACTGTTCCAACGCGATGACTTTTCTGAGGATAGCCATGCCAACGATCCGAAGTTGGTTGGTGATGATTAc < 2:578052/139‑1 (MQ=255)
aCGGCAAATCAACGCAAACCTGCCGGTTGGCGACCTTCCCAACGTTGACACAGAGCGAACTGGCAAACTGTTCCAACGCGATGACTTTTCTGAGGATAGCCATGCCAACGATCCGAAGTTGGTTGGTGATGATTACGTc < 1:402982/139‑1 (MQ=255)
aCGGCAAATCAACGCAAACCTGCCGGTTGGCGACCTTCCCAACGTTGACACAGAGCGAACTGGCAAACTGTTCCAACGCGATGACTTTTCTGAGGATAGCCATGCCAACGATCCGAAGTTGGTTGGTGATGATTACGTc < 1:226434/139‑1 (MQ=255)
aaaTCAACGCAAACCTGCCGGTTGGCGACCTTCCCAACGTTGACACAGAGCGAACTGGCAAACTGTTCCAACGCGATGACTTTTCTGAGGATAGCCATGCCAACGATCCGAAGTTGGTTGGTGATGATTACGTCCCCCa < 1:399100/139‑1 (MQ=255)
aaaTCAACGCAAACCTGCCGGTTGGCGACCTTCCCAACGTTGACACAGAGCGAACTGGCAAACTGTTCCAACGCGATGACTTTTCTGAGGATAGCCATGCCAACGATCCGAAGTTGGTTGGTGATGATTACGTCCCCCa < 1:236468/139‑1 (MQ=255)
aaCGCAAACCTGCCGGTTGGCGACCTTCCCAACGTTGACACAGAGCGAACTGGCAAACTGTTCCAACGCGATGACTTTTCTGAGGATAGCCATGCCAACGATCCGAAGTTGGTTGGTGATGATTACGTCCCCCAGGCAc < 1:54652/139‑1 (MQ=255)
ttGGCGACCTTCCCAACGTTGACACAGAGCGACCTGGCAAACTGTTCCAACGCGATGACTTTTCTGAGGATAGCCATGCCAACGAt > 1:14282/1‑86 (MQ=255)
ttGGCGACCTTCCCAACGTTGACACAGAGCGAACGGGCAAACTGTTCCAACGCGATGACTTTTCTGAGGATAGCCATGCCAACGAt < 2:14282/86‑1 (MQ=255)
tGGCGACCTTCCCAACGTTGACACAGAGCGAACTGGCAAACTGTTCCAACGCGATGACTTTTCTGAGGa < 1:450502/69‑1 (MQ=255)
tGGCGACCTTCCCAACGTTGACACAGAGCGAACTGGCAAACTGTTCCAACGCGATGACTTTTCTGAGGa > 2:450502/1‑69 (MQ=255)
|
AGCAAGGTGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTACGACATGGAACGGCAAATCAACGCAAACCTGCCGGTTGGCGGCCTTCCCAACGTTGACACAGAGCGAACTGGCAAACTGTTCCAACGCGATGACTTTTCTGAGGATAGCCATGCCAACGATCCGAAGTTGGTTGGTGATGATTACGTCCCCCAGGCAC > NC_000913/2562860‑2563100
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
