Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F1 I1 R1
|
82 |
28.6 |
1225488 |
97.4% |
1193625 |
116.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,842,591 |
T→C |
L151L (TTA→TTG) |
hycI ← |
protease involved in processing C‑terminal end of HycE |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,842,591 | 0 | T | C | 100.0%
| 86.5
/ NA
| 26 | L151L (TTA→TTG) | hycI | protease involved in processing C‑terminal end of HycE |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (15/11); total (15/11) |
CGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGGTAGTAAAAGCCGACGATATCCGGCTGAATGCCGAGGAAAATCACTTCG > NC_000913/2842461‑2842726
|
cGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTacgc > 2:435578/1‑139 (MQ=255)
cGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAg < 1:518753/139‑1 (MQ=255)
cccGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATttcctt > 2:162142/1‑139 (MQ=255)
acgcaTCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAg < 2:316058/138‑1 (MQ=255)
cgcaTCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGt < 2:74484/139‑1 (MQ=255)
aTTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCt < 1:110229/139‑1 (MQ=255)
gtgcACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCTCCAACTGCTCTATCCCCCAATTTCCTTCTCAGCCTCCCAGTCGTTGATAAGGGGTTCCACCGGCATCTTTATTCgc > 1:160523/4‑138 (MQ=255)
tCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCt < 2:551835/139‑1 (MQ=255)
ccccATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCGAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTggg > 1:396321/1‑139 (MQ=255)
ccccATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGt < 1:478047/99‑1 (MQ=255)
ccccATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGt > 2:478047/1‑99 (MQ=255)
cccTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTgc < 1:541380/51‑1 (MQ=255)
cccTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTgc > 2:541380/1‑51 (MQ=255)
cccTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAgccgcc > 1:251460/1‑62 (MQ=255)
cccTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAgccgcc < 2:251460/62‑1 (MQ=255)
tCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGGTAGTAAAAGCcg > 1:391219/1‑139 (MQ=255)
aTTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAg < 2:491929/65‑1 (MQ=255)
aTTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAg > 1:491929/1‑65 (MQ=255)
aTTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGGTAGTAAAAGCCGACGat > 1:586034/1‑139 (MQ=255)
aTTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGGTAGTAAAAGCCGACGat > 1:564106/1‑139 (MQ=255)
aTTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGCGTCATCGGGTAGTAAAAGCCGACGat > 2:469279/1‑139 (MQ=255)
ttAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTCTTCGGgtagta < 2:141512/122‑1 (MQ=255)
ttAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGgtagta > 1:141512/1‑122 (MQ=255)
atactCCTCCTCCACCGCCATCTGCGCGAAGCCGCCCTTTACTTCCCGGCCTTCCAGTAGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGGTAGTAAAAGCCGACGATATCCGGCTGAATGCCg < 2:160523/138‑1 (MQ=255)
aCCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGGTAGTAAAAGCCGACGATATCCGGCTGAATGCCGAGGAAAATCACtt > 2:135942/1‑139 (MQ=255)
cGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGGTAGTAAAAGCCGACGATATCCGGCTGAATGCCGAGGAAAATCACTTCg > 2:100965/1‑139 (MQ=255)
|
CGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGGTAGTAAAAGCCGACGATATCCGGCTGAATGCCGAGGAAAATCACTTCG > NC_000913/2842461‑2842726
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
