Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F1 I1 R1 82 28.6 1225488 97.4% 1193625 116.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,073,237 T→C N152S (AAC→AGC)  epd ← D‑erythrose 4‑phosphate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,073,2370TC100.0% 162.4 / NA 45N152S (AAC→AGC) epdD‑erythrose 4‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/18);  total (27/18)

TATGCATCAATAACCTGTTGATCGTGCATGGCGGAGTGAATTGTGGTCACAGTGCCGGACTCAATACCGTACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGTTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACTTTTTTGGCCCCGGCGGCAATATGCGCTTCGCCATG  >  NC_000913/3073111‑3073370
                                                                                                                              |                                                                                                                                     
tATGCATCAATAACCTGTTGATCGTGCATGGCGGAGTGAATTGTGGTCACAGTGCCGGACTCAATACCGTACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGc                                                                                                                           >  1:183734/1‑139 (MQ=255)
tATGCATCAATAACCTGTTGATCGTGCATGGCGGAGTGAATTGTGGTCACAGTGCCGGACTCAATACCGTACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGc                                                                                                                           >  1:340925/1‑139 (MQ=255)
tATGCATCAATAACCTGTTGATCGTGCATGGCGGAGTGAATTGTGGTCACAGTGCCGGACTCAATACCGTACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGc                                                                                                                           >  2:468031/1‑139 (MQ=255)
     aTCAATAACCTGTTGATCGTGCATGGCGGAGTGAATTGTGGTCACAGTGCCGGACTCAATACCGTACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGgtgt                                                                                                                      >  1:578938/1‑139 (MQ=255)
         aTAACCTGTTGATCGTGCATGGCGGAGTGAATTGTGGTCACAGTGCCGGACTCAATACCGTACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCg                                                                                                                          >  2:413163/1‑131 (MQ=255)
         aTAACCTGTTGATCGTGCATGGCGGAGTGAATTGTGGTCACAGTGCCGGACTCAATACCGTACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCg                                                                                                                          <  1:413163/131‑1 (MQ=255)
                 ttGATCGTGCCTGGCGGAGTGAATTGTGGTCACAGTGCCGGACTCAATACCGTACGTATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGACGt                                                                                                          >  1:403520/1‑139 (MQ=255)
                 ttGATCGTGCATGGCGGAGTGAATTGTGGTCACAGTGCCGGACTCAATACCGTACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGt                                                                                                          >  2:554185/1‑139 (MQ=255)
                 ttGATCGTGCATGGCGGAGTGAATTGTGGTCACAGTGCCGGACTCAATACCGTACGCAGCAGCTAACAATTTGATGACGGGAATTAGGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGt                                                                                                          >  1:422186/1‑139 (MQ=255)
                        tGCATGGCGGAGTGAATTGTGGTCACAGTGCCGGACTCAATACCGTACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCt                                                                                                   <  2:340925/139‑1 (MQ=255)
                               cGGAGTGAATTGTGGTCACAGTGCCGGACTCAATACCGTACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGAc                                                                                            >  2:349240/1‑139 (MQ=255)
                                  aGTGAATTGTGGTCACAGTGCCGGACTCAATACCGTACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCATGATTGACGcc                                                                                         >  2:301924/1‑139 (MQ=255)
                                   gTGAATTGTGGTCACAGTGCCGGACTCAATACCGTACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCg                                                                                        >  2:260434/1‑139 (MQ=255)
                                        ttGTGGTCACAGTGCCGGACTCAATACCGTACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAaac                                                                                   >  1:269041/1‑139 (MQ=255)
                                              tCACAGTGCCGGACTCAATACCGTACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGt                                                                             <  2:248786/139‑1 (MQ=255)
                                              tCACAGTGCCGGACTCAATACCGTACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGt                                                                             <  2:183734/139‑1 (MQ=255)
                                                              aaTACCGTACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGTCGCGTCGAGATCGTTa                                                             >  1:501548/1‑139 (MQ=255)
                                                                tACCGTACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGTCGCGTCGAGATCGTTACt                                                           >  2:598628/1‑139 (MQ=255)
                                                                   cGTACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGTCGCGTCGAGATCGTTACTGcc                                                        <  1:494505/139‑1 (MQ=255)
                                                                     tACGCATCATCTAACAATTTTATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGt                                                                             <  1:224249/116‑1 (MQ=255)
                                                                     tACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGt                                                                             >  2:224249/1‑116 (MQ=255)
                                                                     tACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGTCGCGTCga                                                                     >  2:331730/1‑124 (MQ=255)
                                                                     tACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGTCGCGTCga                                                                     <  1:331730/124‑1 (MQ=255)
                                                                     tACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGTCGCGTCGAGATCGTTACTGcc                                                        <  2:210114/137‑1 (MQ=255)
                                                                     tACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGTCGCGTCGAGATCGTTACTGcc                                                        >  1:210114/1‑137 (MQ=255)
                                                                     tACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGTCGCGTCGAGATCGTTACTGCCAg                                                      <  2:578938/139‑1 (MQ=255)
                                                                     tACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTACGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGTCGCGTCGAGATCGTTACTGCCAg                                                      >  1:437129/1‑139 (MQ=255)
                                                                          atcatcTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGTCGCGTCGAGATCGTTACTGCCAGTAtgt                                                 >  1:380047/1‑139 (MQ=255)
                                                                               cTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGTCGCGTCGAGATCGTTACTGCCAGGATGTGaaaa                                            <  1:260434/139‑1 (MQ=255)
                                                                                 aaCAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAaga                                          >  1:444417/1‑139 (MQ=255)
                                                                                          atgaCGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGa                                                                                                             <  2:261969/63‑1 (MQ=255)
                                                                                          atgaCGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGa                                                                                                             >  1:261969/1‑63 (MQ=255)
                                                                                                  aaTTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACTTTTTTGGCCCcg                         <  2:422186/139‑1 (MQ=255)
                                                                                                       tGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACTTTTTTGGCCCCggcggc                    >  1:7643/1‑139 (MQ=255)
                                                                                                        gCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACTTTTTTGGCCCCGGCGGCa                   <  2:501548/139‑1 (MQ=255)
                                                                                                            ttCGTGGTACAGGAAGCGCAAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACTTTTTTGGCCCCGGCGGCAatat               >  2:406821/1‑139 (MQ=255)
                                                                                                              cGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACTTTTTTGGCCCCGGCGGCAATATgc             >  2:557944/1‑139 (MQ=255)
                                                                                                               gTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACTTTTTTGGCCCCGGCGGCAATATgcg            <  1:598628/139‑1 (MQ=255)
                                                                                                                 ggTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACTTTTTTGGCCCCGGCGGCAATATGCGCt          >  1:155780/1‑139 (MQ=255)
                                                                                                                    aCAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGAtt                                                                                               >  2:406230/1‑51 (MQ=255)
                                                                                                                    aCAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGAtt                                                                                               <  1:406230/51‑1 (MQ=255)
                                                                                                                        gAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGTCGCGTCGAGATCGTTACt                                                           <  1:275028/83‑1 (MQ=255)
                                                                                                                        gAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGTCGCGTCGAGATCGTTACt                                                           >  2:275028/1‑83 (MQ=255)
                                                                                                                         aaGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACTTTTTTGGCCCCGGCGGCAATATGCGCTTCGCCATg  <  2:269041/139‑1 (MQ=255)
                                                                                                                         aaGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACTTTTTTGGCCCCGGCGGCAATATGCGCTTCGCCATg  <  1:557944/139‑1 (MQ=255)
                                                                                                                              |                                                                                                                                     
TATGCATCAATAACCTGTTGATCGTGCATGGCGGAGTGAATTGTGGTCACAGTGCCGGACTCAATACCGTACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGTTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACTTTTTTGGCCCCGGCGGCAATATGCGCTTCGCCATG  >  NC_000913/3073111‑3073370

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A