Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F1 I1 R1 82 28.6 1225488 97.4% 1193625 116.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,286,815 T→C H182H (CAT→CAC)  agaI → galactosamine‑6‑phosphate isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,286,8150TC100.0% 61.8 / NA 17H182H (CAT→CAC) agaIgalactosamine‑6‑phosphate isomerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (5/12);  total (5/12)

GATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAACCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATGTTAAAAACCGCGGGTCGCCCCGTGACTCGTGGGATCACCTTAGGCCTGAAGGATATTCTCAATGCCCGCGAAGTTTTGTTACTGGTGACTGGCGAAGGAAAGCAGGATGCGA  >  NC_000913/3286680‑3286933
                                                                                                                                       |                                                                                                                      
gATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAACCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACACgag                                                                                                                     <  1:106545/139‑1 (MQ=255)
       cgcAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAACCGGGAGAAAGCCTGCAACCGGCATGCCATATCAGTCAACTTGATGCCAGAACACATCAACCAGAGATGTTaa                                                                                                              >  1:538997/1‑139 (MQ=255)
                        tttATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAACCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACACGAGATGTTAAAAACCGCGGGTCGcccc                                                                                             <  1:559030/139‑1 (MQ=255)
                          tATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAACCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACACGAGATGTTAAAAACCGCGGGTCGCCCCGt                                                                                           <  1:239221/139‑1 (MQ=255)
                            tGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAACCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACACGAGATGTTAAAAACCGCGGGTCGCCCCGTGa                                                                                         <  2:544790/139‑1 (MQ=255)
                               gTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAACCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACACGAGATGTTAAAAACCGCGGGTCGCCCCGTGACTc                                                                                      <  2:116182/139‑1 (MQ=255)
                               gTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAACCGGGAGAAAGCCTGCAACCGGCCTGCCAGATCAGTCAACTTGATGCCAGAACACAGCAACACGAGATGTTAAAAACCGCGGGTCGCCCCGTGACTc                                                                                      <  2:381225/139‑1 (MQ=255)
                                      gATTGGGGAAAAACGGTCATCTTGGGCTGAACGAACCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACACGAGATGTTAAAAACCGCGGGTCGCCCCGTGACTCGTGGGAt                                                                               <  2:12356/139‑1 (MQ=255)
                                                       cATCTTGGGCTGAACGAACCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACACGAGATGTTAAAAACCGCGGGTCGCCCCGTGACTCGTGGGATCACCTTAGGCCTGAAgg                                                              <  1:130130/139‑1 (MQ=255)
                                                        aTCTTGGGCTGAACGAACCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACACGAGATGTTAAAAACCGCGGGTCGCCCCGTGACTCGTGGGATCACCTTAGGCCTGa                                                                 >  1:551624/1‑135 (MQ=255)
                                                        aTCTTGGGCTGAACGAACCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACACGAGATGTTAAAAACCGCGGGTCGCCCCGTGACTCGTGGGATCACCTTAGGCCTGa                                                                 <  2:551624/135‑1 (MQ=255)
                                                                   aacgaacCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACACGAGATGTTAAAAACCGCGGGTCGCCCCGTGACTCGTGGGATCACCTTAGGCCTGAAGGATATTCTCAATg                                                  >  1:60904/1‑139 (MQ=255)
                                                                                       gCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACACGAGATGTTAAAAACCGCGGGTCGCCCCGTGACTCGTGGGATCACCTTAGGCCTGAAGGATATTCTCAATGCCCGCGAAGTTTTGTTACTg                              <  2:285599/139‑1 (MQ=255)
                                                                                                                cTTGATGCCAGAACACAGCAACACGAGATGTTAAAAACCGCGGGTc                                                                                                  >  1:476382/1‑46 (MQ=255)
                                                                                                                cTTGATGCCAGAACACAGCAACACGAGATGTTAAAAACCGCGGGTc                                                                                                  <  2:476382/46‑1 (MQ=255)
                                                                                                                cTTGATGCCAGAACACAGCAACACGAGATGTTAAAAACCGCGGGTCGCCCCGTGACTCGTGGGATCACCTTAGGCCTGAAgg                                                              >  2:151142/1‑82 (MQ=255)
                                                                                                                cTTGATGCCAGAACACAGCAACACGAGATGTTAAAAACCGCGGGTCGCCCCGTGACTCGTGGGATCACCTTAGGCCTGAAgg                                                              <  1:151142/82‑1 (MQ=255)
                                                                                                                   gATGCCAGAACACAGCAACACGAGATGTTAAAAACCGCGGGTCGCCCCGTGACTCGTGGGATCACCTTAGGCCTGAAGGATATTCTCAATGCCCGCGAAGTTTTGTTACTGGTGACTGGCGAAGGAAAGCAGGATGCGa  >  2:43873/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                      
GATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAACCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATGTTAAAAACCGCGGGTCGCCCCGTGACTCGTGGGATCACCTTAGGCCTGAAGGATATTCTCAATGCCCGCGAAGTTTTGTTACTGGTGACTGGCGAAGGAAAGCAGGATGCGA  >  NC_000913/3286680‑3286933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A