Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F412 I2 R1
|
70 |
105.0 |
5244496 |
94.3% |
4945559 |
100.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,874,193 |
(C)5→6 |
intergenic (‑11/‑159) |
yoaI ← / → yeaL |
uncharacterized protein/UPF0756 family putative inner membrane protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,874,188 | 1 | . | C | 91.8%
| 234.5
/ 15.3
| 73 | intergenic (‑6/‑164) | yoaI/yeaL | uncharacterized protein/UPF0756 family putative inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base . (0/6); new base C (27/40); total (27/46) |
| Fisher's exact test for biased strand distribution p-value = 7.86e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01 |
TTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATG‑CC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGATTTTTTATGCAAAAAACGTAAAG > NC_000913/1874090‑1874282
|
ttCGATAAGAAGAACGGACAACACCTGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATG‑c < 2:2455241/100‑1 (MQ=255)
ttCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATG‑c < 1:925424/100‑1 (MQ=255)
ttCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATG‑c < 1:416451/100‑1 (MQ=255)
tCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATG‑cc > 1:1952661/1‑100 (MQ=255)
gATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATG‑cc‑cc > 2:990009/1‑100 (MQ=255)
gaagaaCGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATG‑CC‑CC‑Ccggg < 2:1324397/100‑2 (MQ=255)
aagaaCGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATG‑CC‑CC‑Ccggga < 1:629401/100‑3 (MQ=255)
aagaaCGGACAAAACCAGTACAAAAAAAAAAAAAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATG‑CC‑CC‑Ccggga < 2:452610/100‑3 (MQ=255)
aagaaCGGACAAAACCAGTACAAAAAAAAAAAAAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATG‑CC‑CC‑Ccggga < 2:1046888/100‑3 (MQ=255)
agaaCGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGgag < 1:540949/100‑1 (MQ=255)
agaaCGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGgag < 1:241501/100‑1 (MQ=255)
aaCGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGAt < 1:1958578/100‑1 (MQ=255)
cGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAg < 1:606569/100‑1 (MQ=255)
ggACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGc > 2:2122845/1‑100 (MQ=255)
cAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGt > 1:452317/1‑100 (MQ=255)
cAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGt > 1:1826295/1‑100 (MQ=255)
aaaaCCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGtt < 1:1162646/100‑1 (MQ=255)
aaaCCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGttt > 2:173582/1‑100 (MQ=255)
aaaCCAGTACAACAGAAAAAGAAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGttt < 2:776455/100‑1 (MQ=255)
taCCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGtttt < 2:409216/99‑1 (MQ=255)
aCCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGtttta > 1:417643/1‑99 (MQ=255)
ccAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCa > 2:2438605/1‑100 (MQ=255)
cAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCAc < 1:596722/100‑1 (MQ=255)
aGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACg < 2:678660/100‑1 (MQ=255)
gTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGt < 2:869898/100‑1 (MQ=255)
gTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGt < 2:564122/100‑1 (MQ=255)
acaacaGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTa < 2:776267/100‑1 (MQ=255)
cAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAg < 1:1606943/100‑1 (MQ=255)
aaTGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGc > 2:1083400/1‑100 (MQ=255)
aTGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCg < 2:1467110/100‑1 (MQ=255)
aTGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCg > 1:443199/1‑100 (MQ=255)
aTGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCg < 2:1342371/100‑1 (MQ=255)
aTGGCAAAAAACGATGACGTGATAACCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCg < 1:2166663/100‑1 (MQ=255)
tGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGt > 2:452654/1‑100 (MQ=255)
ggCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTg < 1:1342726/100‑1 (MQ=255)
cAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGca > 1:204531/1‑100 (MQ=255)
cAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGca < 1:947972/100‑1 (MQ=255)
aaaaaaCGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGcac < 2:2559772/100‑1 (MQ=255)
aaaaaaCGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGcac < 2:2400036/100‑1 (MQ=255)
aaaaaaCGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGACATAGCTGTTTTCACGTTATTGTTACCGTCcac > 2:2512628/1‑100 (MQ=255)
aaaaaCGATCACGTGATAATCAGTGTCTCGACAAACATTTGAGCGTTCATAGCACG‑CC‑CCTCGGGAGATAGCTGTTTTCACGTTCTTGTTAGCGTGcaca < 1:2300244/100‑1 (MQ=255)
aaaCGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACaaa < 1:608994/100‑1 (MQ=255)
aaaCGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACaaa > 1:92404/1‑100 (MQ=255)
cGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATgg > 2:1310345/1‑100 (MQ=255)
aCGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGtt < 1:1569520/100‑1 (MQ=255)
cGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGttt > 2:886690/1‑100 (MQ=255)
gTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTtg < 2:1266171/100‑1 (MQ=255)
gTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTtg < 2:421092/100‑1 (MQ=255)
gTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTtg > 1:1677488/1‑100 (MQ=255)
taTAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGTGATAGCTGTTTTCACGATATTGTTAGAGCACACTCATCGCAGttgctc < 2:599167/99‑5 (MQ=255)
aaTCAGTGTCTCGACAAACATTTGCTCGTCCATAGCATG‑CCACC‑CGGGAGATAGCTGTGTTCACGTTATTGTTAGCGTGCACAAATGGCAGTCTGATGAc < 1:2242022/100‑1 (MQ=255)
aaTCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGAc < 1:1683430/100‑1 (MQ=255)
aaTCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGAc < 1:736141/100‑1 (MQ=255)
aTCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGCTAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACa > 2:2493316/1‑100 (MQ=255)
tCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAg < 1:741729/100‑1 (MQ=255)
tgtCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCg < 1:160621/100‑1 (MQ=255)
tctcGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGcc < 2:91033/100‑1 (MQ=255)
ctcGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCt > 1:1467399/1‑100 (MQ=255)
cGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGa > 2:514843/1‑100 (MQ=255)
cAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGAttt < 2:105739/100‑1 (MQ=255)
cAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGAttt > 1:1580241/1‑100 (MQ=255)
aaCATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGAttttt > 2:786621/1‑100 (MQ=255)
aaCATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGAttttt < 1:2207091/100‑1 (MQ=255)
cATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGATTTTTTa < 1:1724191/100‑1 (MQ=255)
cATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGATTTTTTa < 1:1724183/100‑1 (MQ=255)
tttGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGATTTTTTATg > 1:1275799/1‑100 (MQ=255)
gATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGATTTTTTATGCaa > 2:1434726/1‑100 (MQ=255)
gATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGATTTTTTATGCaa > 2:1414376/1‑100 (MQ=255)
gATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGATTTTTTATGCaa > 2:98798/1‑100 (MQ=255)
gTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGATTTTTTATGCaaaaaa > 1:495964/1‑100 (MQ=255)
gTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGATTTTTTATGCaaaaaa > 1:1175796/1‑100 (MQ=255)
ttCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGATTTTTTATGCAAAAAAc < 1:423224/100‑1 (MQ=255)
ttCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGATTTTTTATGCAAAAAAc < 2:115152/100‑1 (MQ=255)
tCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGATTTTTTATGCAAAAAACg < 1:401564/100‑1 (MQ=255)
cATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGATTTTTTATGCAAAAAACGt < 1:1767867/100‑1 (MQ=255)
aTAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGATTTTTTATGCAAAAAACGTa < 2:1724783/100‑1 (MQ=255)
aGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGATTTTTTATGCAAAAAACGTaaa < 1:2179403/100‑1 (MQ=255)
gCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGATTTTTTATGCAACAAACGTAAAg > 2:370878/1‑100 (MQ=255)
|
TTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATG‑CC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGATTTTTTATGCAAAAAACGTAAAG > NC_000913/1874090‑1874282
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
