Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F412 I2 R1 70 105.0 5244496 94.3% 4945559 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,650,949 G→A H447Y (CAC→TAC)  yfhM ← bacterial alpha2‑macroglobulin colonization factor ECAM; anti‑host protease defense factor; periplasmic inner membrane‑anchored lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,650,9490GA98.0% 333.2 / ‑4.5 102H447Y (CAC→TAC) yfhMbacterial alpha2‑macroglobulin colonization factor ECAM; anti‑host protease defense factor; periplasmic inner membrane‑anchored lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base A (55/45);  total (57/45)
Fisher's exact test for biased strand distribution p-value = 5.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 105 total aligned reads displayed.

ACGTGGAAATCCCACATCCGATACTGATTATCGCCCGTGTTAGCGCGAATATGCCACATACCGGTTGCCGCATTGCTATCGAGTGGCCAGGTAAAGTGGTAGAGGCCATTCTCCGGCTGACTAACGACGCTCCTGAGTACCTGCCCATCGGGTTTAATCACGTCTAACTTGATGGGTTGATTGGGCAACGCTTTACC  >  NC_000913/2650852‑2651048
                                                                                                 |                                                                                                   
aCGTGGAAATCCCACATCCGATACTGATTATCGCCCGTGTTAGCGCGAATATGCCACATACCGGTTGCCGCATTGCTATCGAGTGGCCAGGTAAagtagt                                                                                                   <  2:784384/100‑1 (MQ=255)
    ggAAATCCCACATCCGATACTGATTATCGCCCGTGTTAGCGCGAATATGCCACATACCGGTTGCCGCATTGCTATCGAGTGGCCAGGTAAAGTAGTagag                                                                                               >  1:1983873/1‑100 (MQ=255)
    ggAAATCCCACATCCGATACTGATTATCGCCCGTGTTAGCGCGAATATGCCACATACCCGTTGCCGCATTGCTATCGAGTGGCCAGGTAAAGTAGTagag                                                                                               >  2:1696812/1‑100 (MQ=255)
     gAAATCCCACATCCGATACTGATTATCGCCCGTGTTAGCGCGAATATGCCACATACCGGTTGCCGCATTGCTATCGAGTGGCCAGGTAAAGTAGTAGAgg                                                                                              <  1:2599034/100‑1 (MQ=255)
      aaaTCCCACATCCGATACTGATTATCGCCCGTGTTAGCGCGAATATGCCACATACCGGTTGCCGCATTGCTATCGAGTGGCCAGGTAAAGTAGTAGAGGc                                                                                             <  1:1269173/100‑1 (MQ=255)
      aaaTCCCACATCCGATACTGATTATCGCCCGTGTTAGCGCGAATATGCCACATACCGGTTGCCGCATTGCTATCGAGTGGCCAGGTAAAGTAGTAGAGGc                                                                                             <  2:1157486/100‑1 (MQ=255)
      aaaTCCCACATCCGATACTGATTATCGCCCGTGTTAGCGCGAATATGCCACATACCGGTTGCCGCATTGCTATCGAGTGGCCAGGTAAAGTAGCAGAGGc                                                                                             <  1:775349/100‑1 (MQ=255)
         tCCCACATCCGATACTGATTATCGCCCGTGTTAGCGCGAATATGCCACATACCGGTTGCCGCATTGCTATCGAGTGGCCAGGTAAAGTAGTAGAGGCCAt                                                                                          >  2:2093376/1‑100 (MQ=255)
           ccACATCCGATACTGATTATCGCCCGTGTTAGCGCGAATATGCCACATACCGGTTGCCGCATTGCTATCGAGTGGCCAGGTAAAGTAGTAGAGGCCATtc                                                                                        >  2:2486938/1‑100 (MQ=255)
             acaTCCGATACTGATTATCGCCCGTGTTAGCGCGAATATGCCACATACCGGTTGCCGCATTGCTATCGAGTGGCAAGGTAAAGTAGTAGAGGCCATtctc                                                                                      >  2:792092/1‑100 (MQ=255)
              caTCCGATACTGATTATCGCCCGTGTTAGCGCGAATATGCCACATACCGGTTGCCGCATTGCTATCGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTcc                                                                                     <  2:1534058/100‑1 (MQ=255)
                tCCGATACTGATTATCGCCCGTGTTAGCGCGAATATGCCACATACCGGTTGCCGCATTGCTATCGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTCCgg                                                                                   >  1:1237600/1‑100 (MQ=255)
                tCCGATACTGATTATCGCCCGTGTTAGCGCGAATATGCCACATACCGGTTGCCGCATTGCTATCGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTCCgg                                                                                   >  2:694282/1‑100 (MQ=255)
                tCCGATACTGATTATCGCCCGTGTTAGCGCGAATATGCCACATACCGGTTGCCGCATTGCTATCGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTCCgg                                                                                   >  1:2227933/1‑100 (MQ=255)
                tCCGATACTGATTATCGCCCGTGTTAGCGCGAATATGCCACATACCGGTTGCCGCATTGCTATCGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTCCgg                                                                                   >  2:24947/1‑100 (MQ=255)
                 ccGATACTGATTATCGCCCGTGTTAGCGCGAATATGCCACATACCGGTTGCCGCATTGCTATCGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTCCGGc                                                                                  >  2:1457183/1‑100 (MQ=255)
                 ccGATACTGATTATCGCCCGTGTTAGCGCGAATATGCCACATACCGGTTGCCGCATTGCTATCGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTCCGGc                                                                                  <  1:115957/100‑1 (MQ=255)
                 ccGATACTGATTATCGCCCGTGTTAGCGCGAATATGCCACATACCGGTTGCCGCATTGCTATCGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTCCGGc                                                                                  >  2:2568486/1‑100 (MQ=255)
                 atcttACTGATTATCGCCCGTGTTAGCGCGAATATGCCACATACCGGTTGCCGCATTGCTATCGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTCCGGc                                                                                  <  2:2384682/96‑1 (MQ=255)
                  cGATACTGATTATCGCCCGTGTTAGCGCGAATATGCCACATACCGGTTGCCGCATTGCTATCGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTCCGGCt                                                                                 >  1:1334490/1‑100 (MQ=255)
                  cGATACTGATTATCGCCCGTGTTAGCGCGAATATGCCACATACCGGTTGCCGCATTGCTATCGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTCCGGCt                                                                                 <  2:1775496/100‑1 (MQ=255)
                  cGATACTGATTATCGCCCGTGTTAGCGCGAATATGCCAAAGAGCGGTTGCCGCATTGATAACGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTCAGGCt                                                                                 >  2:327463/1‑100 (MQ=255)
                   gATACTGATTATCGCCCGTGTTAGCGCGAATATGCCACATACCGGTTGCCGCATTGCTATCGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTCCGGCTg                                                                                <  1:1769585/100‑1 (MQ=255)
                   gATACTGATTATCGCCCGTGTTAGCGCGAATATGCCACATACCGGTTGCCGCATTGCTATCGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTCCGGCTg                                                                                <  1:1310886/100‑1 (MQ=255)
                   gATACTGATTATCGCCCGTGTTAGCGCGAATATGCCACATACCGGTTGCCGCATTGCTATCGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTCCGGCTg                                                                                <  2:2018438/100‑1 (MQ=255)
                     tACTGATTATCGCCCGTGTTAGCGCGAATATGCCACATACCGGTTGCCGCATTGCTATCGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTCCGGcagat                                                                              >  1:2430348/1‑96 (MQ=255)
                     tACTGATTATCGCCCGTGTTAGCGCGAATATGCCACATACCGGTTGCCGCATTGCTATCGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTCCGGCTGAc                                                                              >  1:1728725/1‑100 (MQ=255)
                     tACTGATTATCGCCCGTGTTAGCGCGAATATGCCACATACCGGTTGCCGCATTGCTATCGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTCCGGCTGAc                                                                              <  2:2048351/100‑1 (MQ=255)
                        tGATTATCGCCCGTGTTAGCGCGAATATGCCACATACCGGTTGCCGCATTGCTATCGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTCCGGCTGACTaa                                                                           >  1:2147069/1‑100 (MQ=255)
                         gATTATCGCCCGTGTTAGCGCGAATATGCCATATACCGGTTGCCGCATTGCTATCGAGTGGTCAGGTAAAGTAGTAGAGGCCATTCTCCGGCTGACTAac                                                                          <  2:451696/100‑1 (MQ=255)
                             aTCGCCCGTGTTAGCGCGAATATGCCACATACCGGTTGCCGCATTGCTATCGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTCCGGCTGACTAacgacg                                                                      >  1:1581305/1‑100 (MQ=255)
                                gCCCGTGTTAGCGCGAATATGCCACATACCGGTTGCCGCATTGCTATCGAGTGGCCAGGTAAAGTGGTAGAGGCCATTCTCCGGCTGACTAACGACGCTc                                                                   >  1:1286127/1‑100 (MQ=255)
                                gCCCGTGTTAGCGCGAATATGCCACATACCGGTTGCCGCATTGCTATCGAGTGGCCAGGGAAAGTAGTAGAGGCCATTCTCCGGCTGACTAACGACGCTc                                                                   >  1:1975318/1‑100 (MQ=255)
                                 ctcGTGTTAGCGCGAATATGCCACATACCGGTTGCCGCATTGCTATCGAGTTGCCAGGTAAAGTAGTAGAGGTCATTCTCCGGCTGACTAACGACGCTcc                                                                  <  2:2582036/98‑1 (MQ=255)
                                 cccGTGTTAGCGCGAATATGCCACATACCGGTTGCCGCATTGCTATCGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTCCGGCTGACTAACGACGCTcc                                                                  <  2:814386/100‑1 (MQ=255)
                                 cccGTGTTAGCGCGAATATGCCACATACCGGTTGCCGCATTGCTATCGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTCCGGCTGACTAACGACGCTcc                                                                  <  1:318588/100‑1 (MQ=255)
                                   cGTGTTAGCGCGAATATGCCACATACCGGTTGCCGCATTGCTATCGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTCCGGCTGACTAACGACGCTCCTg                                                                <  2:463716/100‑1 (MQ=255)
                                    gtgtTAGCGCGAATATGCCACATACCGGTTGCCGCATTGCTATCGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTCCGGCTGACTAACGACGCTCCTGa                                                               >  1:497550/1‑100 (MQ=255)
                                     tgtTAGCGCGAATATGCCACATGCCGGTTGCCGCATTGCTATCGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTCCGGCTGACTAACGACGCTCCTGAg                                                              <  2:2157770/100‑1 (MQ=255)
                                     tgtTAGCGCGAATATGCCACATACCGGTTGCCGCATTGCTATCGAGTGGCCAGTTAAAGTGGTAGAGGCCAGTCTCCGGCTGCCTAATGGCGGTACTGAg                                                              >  2:601267/1‑100 (MQ=255)
                                     tgtTAGCGCGAATATGCCACATACCGGTTGCCGCATTGCTATCGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTCCGGCTGACTAACGACGCTCCTGAg                                                              <  1:481208/100‑1 (MQ=255)
                                      gtTAGCGCGAATATGCCACATACCGGTTGCCGCATTGCTATCGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTCCGGCTGACTAACGACGCTCCTGAGt                                                             >  1:1631373/1‑100 (MQ=255)
                                        tAGCGCGAATATGCCACATACCGGTTGCCGCATTGCTATCGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTCCGGCTGACTAACGACGCTCCTGAGTAc                                                           <  2:176400/100‑1 (MQ=255)
                                         aGCGCGAATATGCCACATGCCGGTTGCCGCATTGCTATCGAGTGGCCAGGTAAAGTAGTAGAGGCAATTATCCGGCTGACTAACGACGCTCCTGACTAcc                                                          >  2:386465/1‑100 (MQ=255)
                                         aGCGCGAATATGCCACATACCGGTTGCCGCATTGCTATCGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTCCGGCTGACTAACGACGCTCCTGAGTAcc                                                          <  2:767909/100‑1 (MQ=255)
                                            gcgAATATGCCACATACCGGTTGCCGCATTGCTATCGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTCCGGCTGACTAACGACGCTCCTGAGTACCTGc                                                       >  1:305389/1‑100 (MQ=255)
                                             cgAATATGCCACATACCGGTTGCCGCATTGCTATCGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTCCGGCTGACTAACGACGCTCCTGAGTACCTGcc                                                      <  1:1980749/100‑1 (MQ=255)
                                             cgAATATGCCACATACCGGTTGCCGCATTGCTATCGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTCCGGCTGACTAACGACGCTCCTGAGTACCTGcc                                                      <  2:2141682/100‑1 (MQ=255)
                                             cgAATATGCCACATACCGGTTGCCGCATTGCTATCGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTCCGGCTGACTAACGACGCTCCTGAGTACCTGcc                                                      <  1:1389228/100‑1 (MQ=255)
                                                  atGCCACATACCGGTTGCCGCATTGCTATCGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTCCGGCTGACTAACGACGCTCCTGAGTACCTGCCCATCg                                                 <  2:1712448/100‑1 (MQ=255)
                                                   tGCCACATACCGGTTGCCGCATTGCTATCGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTCCGGCTGACTAACGACGCTCCTGAGTACCTGCCCATCgg                                                >  1:1145075/1‑100 (MQ=255)
                                                   tGCCACATACCGGTTGCCGCATTGCTATCGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTCCGGCTGACTAACGACGCTCCTGAGTACCTGCCCATCgg                                                <  1:1206354/100‑1 (MQ=255)
                                                    gCCACATACCGGTTGCCGCATTGCTATCGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTCCGGCTGACTAACGACGCGCCTGAGTACCTGCCCATCggg                                               <  2:27218/100‑1 (MQ=255)
                                                     ccACATACCGGTTGCCGCATTGCTATCGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTCCGGCTGACTAACGACGCTCCTGAGTACCTGCCCATCggct                                              >  2:1010084/1‑98 (MQ=255)
                                                      cacaTACCGGTTGCCGCATTGCTATCGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTCCGGCTGACTAACGACGCTCCTGAGTACCTGCCCATCGGGtt                                             <  2:131718/100‑1 (MQ=255)
                                                      cacaTACCGGTTGCCGCATTGCTATCGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTCCGGCTGACTAACGACGCTCCTGAGTACCTGCCCATCGGGtt                                             <  2:229748/100‑1 (MQ=255)
                                                      cacaTACCGGTTGCCGCATTGCTATCGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTCCGGCTGACTAACGACGCTCCTGAGTACCTGCCCATCGGGtt                                             >  1:221047/1‑100 (MQ=255)
                                                      cacaTACCGGTTGCCGCATTGCTATCGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTCCGGCTGACTAACGACGCTCCTGAGTACCTGCCCATCGGGtt                                             <  2:1866485/100‑1 (MQ=255)
                                                       acaTACCGGTTGCCGCATTGCTATCGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTCCGGCTGACTAACGACGCTCCTGAGTACCTGCCCATCGGGttt                                            >  1:2571400/1‑100 (MQ=255)
                                                        caTACCGGTTGCCGCATTGCTATCGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTCCGGCTGACTAACGACGCTCCTGAGTACCTGCCCATCGGGTTTa                                           >  1:77588/1‑100 (MQ=255)
                                                         aTACCGGTTGCCGCATTGCTATCGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTCCGGCTGACTAACGACGCTCCTGAGTACCTGCCCATCGGGTTTaa                                          >  2:2293699/1‑100 (MQ=255)
                                                         aTACCGGTTGCCGCATTGCTATCGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTCCGGCTGACTAACGACGCTCCTGAGTACCTGCCCATCGGGTTTaa                                          <  1:708024/100‑1 (MQ=255)
                                                           aCCGGTTGCCGCATTGCTATCGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTCCGGCTGACTAACGACGCTCCTGAGTACCTGCCCATCGGGTTTAATc                                        <  1:86308/100‑1 (MQ=255)
                                                              ggTTGCCGCATTGCTATCGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTCCGGCTGACTAACGACGCTCCTGAGTACCTGCCCATCGGGTTTAATCACg                                     >  1:1856971/1‑100 (MQ=255)
                                                              ggTTGCCGCATTGCTATCGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTCCGGCTGACTAACGACGCTCCTGAGTACCTGCCCATCGGGTTTAATCACg                                     <  1:2407371/100‑1 (MQ=255)
                                                              ggTTGCCGCATTGCTATCGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTCCGGCTGACTAACGACGCTCCTGAGTACCTGCCCATCGGGTTTAATCACg                                     <  2:1165582/100‑1 (MQ=255)
                                                              ggTTGCCGCATTGCTATCGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTCCGGCTGACTAACGACGCTCCTGAGTACCTGCCCATCGGGTTTAATCACg                                     >  1:939582/1‑100 (MQ=255)
                                                                 tGCCGCATTGCTATCGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTCCGGCTGACTAACGACGCTCCTGAGTACCTGCCCATCGGGTTTAATCACGTCt                                  <  2:1698119/100‑1 (MQ=255)
                                                                   ccGCATTGCTATCGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTCCGGCTGACTAACGACGCTCCTGAGTACCTGCCCATCGGGTTTAATCACGTCTaa                                >  2:2312023/1‑100 (MQ=255)
                                                                   ccGCATTGCTATCGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTCCGGCCGACTAACGACGCTCCTCAGTACCTGCCCACCGGGTTTAATCACGTCTaa                                >  2:722911/1‑100 (MQ=255)
                                                                    cGCATTGCTATCGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTCCGGCTGACTAACGACGCTCCTGAGTACCTGCCCATCGGGTTTAATCACGTCTAAc                               >  1:2554773/1‑100 (MQ=255)
                                                                     gCATTGCTATCGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTCCGGCTGACTAACGACGCTCCTGAGTACCTGCCCATCGGGTTTAATCACGTCTAACt                              >  1:400130/1‑100 (MQ=255)
                                                                         tGCTATCGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTCCGGCTGACTAACGACGCTCCTGAGTACCTGCCCATCGGGTTTAATCACGTCTAACTTGAt                          >  1:2440542/1‑100 (MQ=255)
                                                                          gCTATCGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTCCGGCTGACTAACGACGCTCCTGAGTACCTGCCCATCGGGTTTAATCACGTCTAACTTGATg                         <  1:1209047/100‑1 (MQ=255)
                                                                          gCTATCGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTCCGGCTGACTAACGACGCTCCTGAGTACCTGCCCATCGGGTTTAATCACGTCTAACTTGATg                         <  1:1209119/100‑1 (MQ=255)
                                                                          gCTATCGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTCCGGCTGACTAACGACGCTCCTGAGTACCTGCCCATCGGGTTTAATCACGTCTAACTTGATg                         >  2:1401934/1‑100 (MQ=255)
                                                                           cTATCGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTCCGGCTGACTAATGACGCTCCTGAGTACCTGCCCATCGGGTTTAATCACGTCTAACTTGATgg                        >  2:265497/1‑100 (MQ=255)
                                                                           cTATCGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTCCGGCTGACTAATGACGCTCCTGAGTACCTGCCCATCGGGTTTAATCACGTCTAACTTGATgg                        >  2:266234/1‑100 (MQ=255)
                                                                           cTATCGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTCCGGCTGACTAACGACGCTCCTGAGTACCTGCCCATCGGGTTTAATCACGTCTAACTTGATgg                        <  1:759377/100‑1 (MQ=255)
                                                                           cTATCGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTCCGGCTGACTAACGACGCTCCTGAGTACCTGCCCATCGGGTTTAATCACGTCTAACTTGATgg                        <  1:394264/100‑1 (MQ=255)
                                                                           cTATCGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTCCGGCTGACTAACGACGCTCCTGAGTACCTGCCCATCGGGTTTAATCACGTCTAACTTGATgg                        >  1:1829911/1‑100 (MQ=255)
                                                                           cTATCGAGTGGCCAGGGAAAGTAGCAGAGGCCCTACTCCGGCTGACTAACGACACGCCTGAGTACCTGCCCATCGGGTTTAATCACGTCTAAGTTTATgg                        >  2:1131054/1‑100 (MQ=255)
                                                                            tATCGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTCCGGCTGACTAACGACGCTCCTGAGTACCTGCCCATCGGGTTTAATCACGTCTAACTTGATggg                       >  2:2023392/1‑100 (MQ=255)
                                                                             aTCGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTCCGGCTGACTAACGACGCTCCTGAGTACCTGCCCATCGGGTTTAATCACGTCTAACTTGATGGGt                      >  2:1620617/1‑100 (MQ=255)
                                                                              tCGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTCCGGCTGACTAACGACGCTCCTGAGTACCTGCCCATCGGGTTTAATCACGTCTAACTTGATGGGtt                     <  2:1539371/100‑1 (MQ=255)
                                                                               cGAGTGGCCAGGTAAAGTAGTAGAGGCCATTCTCCGGCTGACTAACGACGCTCCTGAGTACCTGCCCATCGGGTTTAATCACGTCTAACTTGATGGGTTg                    >  1:1538087/1‑100 (MQ=255)
                                                                                  gTGGCCAGGTAAAGTAGTAGAGCCTCTTCTCCGGCTGACTAACGACGCTCCTGAGTAACTGCACATCGGGTTTAATCACGCCTAACGCGAGGGGTTGAtt                 >  2:200812/1‑100 (MQ=255)
                                                                                   tGGCCAGGTAAAGTAGTAGAGGCCATTCTCCGGCTGACTAACGACGCTCCTGAGTACCTGCCCATCGGGTTTAATCACGTCTAACTTGATGGGTTGATTg                <  2:31329/100‑1 (MQ=255)
                                                                                    ggCCAGGTAAAGTAGTAGAGGCCATTCTCCGGCTGACTAACGACGCTCCTGAGTACCTGCCCATCGGGTTTACTCACGTCTAACTTGATGGGTTGATTgg               >  1:1579267/1‑100 (MQ=255)
                                                                                    ggCCAGGTAAAGTAGTAGAGGCCATTCTCCGGCTGACTAACGACGCTCCTGAGTACCTGCCCATCGGGTTTAATCACGTCTAACTTGATGGGTTGATTgg               >  2:259767/1‑100 (MQ=255)
                                                                                     gCCAGGTTAAGTAGTAGAGGCCATTCTCCGGCTGACTAACGACGCTCCTGAGTACCTGCCCATCGGGTTTAATCACGTCTAACTTGATGGGTTGATTggg              >  2:2045970/1‑100 (MQ=255)
                                                                                     gCCAGGTAAAGTAGTAGAGGCCATTCTCCGGCTGACTAACGACGCTCCTGAGTACCTGCCCATCGGGTTTAATCACGTCTAACTTGATGGGTTGATTggg              <  2:2429211/100‑1 (MQ=255)
                                                                                     gCCAGGTAAAGTAGTAGAGGCCATTCTCCGGCTGACTAACGACGCTCCTGAGTACCTGCCCATCGGGTTTAATCACGTCTAACTTGATGGGTTGATTggg              >  1:2552317/1‑100 (MQ=255)
                                                                                       cAGGTAAAGTAGTAGAGGCCATTCTCCGGCTGACTAACGACGCTCCTGAGTACCTGCCCATCGGGTTTAATCACGTCTAACTTGATGGGTTGATTGGGCa            >  2:1034131/1‑100 (MQ=255)
                                                                                       cAGGTAAAGTAGTAGAGGCCATTCTCCGGCTGACTAACGACGCGCCTGAGTACCTGCCCATCGGGTTTAATCACGTCTAACTTGATGGGTTGATTGGGCa            >  2:1592334/1‑100 (MQ=255)
                                                                                          gTAAAGTAGTAGAGGCCATTCTCCGGCTGACTAACGACGCTCCTGAGTACCTGCCCATCGGGTTTAATCACGTCTAACTTGATGGGTTGATTGGGCAACg         >  1:1251165/1‑100 (MQ=255)
                                                                                          gTAAAGTAGTAGAGGCCATTCTCCGGCTGACTAACGACGCGCCTGAGTACCTGCCCATCGGGTTTAATCACGTCTAACTTGATGGGTTGATTGGGCAACg         >  2:451356/1‑100 (MQ=255)
                                                                                             aaGTAGTAGAGGCCATTCTCCGGCTGACTAACGACGCTCCTGAGTACCTGCCCATCGGGTTTAATCACGTCTAACTTGATGGGTTGATTGGGCAACGCtt      <  2:1507923/100‑1 (MQ=255)
                                                                                              agtagtAGAGGCCATTCTCCGGCTGACTAACGACGCTCCTGAGTACCTGCCCATCGGGTTTAATCACGTCTAACTTGATGGGTTGATTGGGCAACGCttt     >  2:1474687/1‑100 (MQ=255)
                                                                                                 ggTAGAGGCCATTCTCCGGCTGACTAACGACGCTCCTGAGTACCTGCCCATCGGGTTTAATCACGTCTAACTTGATGGGTTGATTGGGCAACGCTTTAcc  <  2:1267564/100‑1 (MQ=255)
                                                                                                 |                                                                                                   
ACGTGGAAATCCCACATCCGATACTGATTATCGCCCGTGTTAGCGCGAATATGCCACATACCGGTTGCCGCATTGCTATCGAGTGGCCAGGTAAAGTGGTAGAGGCCATTCTCCGGCTGACTAACGACGCTCCTGAGTACCTGCCCATCGGGTTTAATCACGTCTAACTTGATGGGTTGATTGGGCAACGCTTTACC  >  NC_000913/2650852‑2651048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A