Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F412 I2 R1
|
70 |
105.0 |
5244496 |
94.3% |
4945559 |
100.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,669,936 |
G→A |
R302H (CGC→CAC) |
hcaE → |
3‑phenylpropionate dioxygenase, large (alpha) subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,669,936 | 0 | G | A | 97.4%
| 244.6
/ NA
| 77 | R302H (CGC→CAC) | hcaE | 3‑phenylpropionate dioxygenase, large (alpha) subunit |
| Reads supporting (aligned to +/- strand): ref base G (0/1); major base A (42/32); minor base C (0/2); total (42/35) |
| Fisher's exact test for biased strand distribution p-value = 1.97e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GGATGCTAATGTCTGGGTCGATGGCGCAGTTTCAAGCTATTACCGCGAAACCTATGCCGAAGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCGCCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGGCCCTGATCAAGTTG > NC_000913/2669841‑2670031
|
ggATGCTAATGTCTGGGTCGATGGCGCAGTTTCAAGCTATTACCGCGAAACCTATGCCGAAGCAGAGCAACGTTTAGGTGAAGTTCGCGCCCTGCACCTg > 2:271323/1‑100 (MQ=255)
gCTAATGTCTGGGTCGATGGCGCAGTTTCAAGCTATTACCGCGAAACCTATGCCGAAGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCACCTGGCgg > 1:2150506/1‑100 (MQ=255)
cTAATGTCTGGGTCGATGGCGCAGTTTCAAGCTATTACCGCGAAACCTATGCCGAAGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCACCTGGCggg < 1:1250270/100‑1 (MQ=255)
cTAATGTCTGGGTCGATGGCGCAGTTTCAAGCTATTACCGCGAAACCTATGCCGAAGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCACCTGGCggg < 2:1895888/100‑1 (MQ=255)
cTAATGTCTGGGTCGAGTGCGCAGTTTCAAGCTATTACCGCTAAACCTATGCCGAAGCAGAACAACGTTTATGTTAAGTTCGCGCCCTGCACCTGGCggg < 2:1557960/100‑1 (MQ=255)
tAATGTTTGGGTCGGTGGCGCAGGTGAACGCTATTACCGCGAAACCTAGGCCGAAGCAGAACAACGGTTAGGTGAAGTTCGCGCCCTGCACCTGGCGGGt < 2:1668966/100‑1 (MQ=255)
tAATGTCTGGGTCGATGGCGCAGTTTCAAGCTATTACCGCGAAACCTATGCCGAAGCAGAACACCGTTTAGGTGACGTTCGCGCCCTGCACCTCGCGGGt > 2:2061799/1‑100 (MQ=255)
aaTGTCTGGGTCGATGGCGCAGTTTCAAGCTATTACCGCGAAACCTATGCCGAAGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCACCTGGCGGGTc > 2:964380/1‑100 (MQ=255)
aaTGTCTGGGTCGATGGCGCAGTTTCAAGCTATTACCGCGAAACCTATGCCGAAGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCACCTGGCGGGTc < 1:2028245/100‑1 (MQ=255)
aTGTCTGGGTCGATGGCGCAGTTTCAAGCTATTACCGCGAAACCTATGCCGAAGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCACCTGGCGGGTCa < 1:969563/100‑1 (MQ=255)
aTGTCTGGGTCGATGGCGCAGTTTCAAGCTATTACCGCGAAACCTATGCCGAAGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCACCTGGCGGGTCa < 2:263920/100‑1 (MQ=255)
tGTCTGGGTCGATGGCGCAGTTTCAAGCTATTACCGCGAAACCTATGCCGAAGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCACCTGGCGGGTCAt < 2:2131457/100‑1 (MQ=255)
tGTCTGGGTCGATGGCGCAGTTTCAAGCTATTACCGCGAAACCTATGCCGAAGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCACCTGGCGGGTCAt > 1:248475/1‑100 (MQ=255)
gTCTGGGTCGATGGCGCAGTTTCAAGCTATTACCGCGAAACCTATGCCGAAGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCACCTGGCGGGTCATa < 2:742572/100‑1 (MQ=255)
gTCTGGGTCGATGGCGCAGTTTCAAGCTATTACCGCGAAACCTATGCCGAAGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCACCTGGCGGGTCATa > 2:1780174/1‑100 (MQ=255)
tCTGGGTCGATGGCGCAGTTTCAAGCTATTACCGCGAAACCTATGCCGCCGGAGAACAAAGTTTAGGTGGAGTTCGCGACCTGCCTCTGGCGGGTAATac < 2:1296233/100‑2 (MQ=255)
tCTGGGTCGATGGCGCAGTTTCAAGCTATTACCGCGAAACCTATGCCGAAGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCACCTGGCGGGTCATaa < 2:2372586/100‑1 (MQ=255)
cTGGGTCGATGGCGCAGTTTCAAGCTATTACCGCGAAACCTATGCCGAAGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCACCTGGCGGGTCATAAc < 1:1136460/100‑1 (MQ=255)
ggTCGATGGCGCAGTTTCAAGCTATTACCGCGAAACCTATGCCGAAGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCACCTGGCGGGTCATAACAat > 2:1866350/1‑100 (MQ=255)
ggTCGATGGCGCAGTTTCAAGCTATTACCGCGAAACCTATGCAGAAGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCACCTGGCGGGTCATAACAat > 2:355180/1‑100 (MQ=255)
ggCGCAGTTTCAAGCTATTACCGCGAAACCTCTGCCGAAGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCACCTGGCGGGTCATAACAATATTTTcc < 1:2483490/100‑1 (MQ=255)
ggCGCAGTTTCAAGCTATTACCGCGAAACCTATGCCGAAGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCACCTGGCGGGTCATAACAATATTTTcc < 1:744328/100‑1 (MQ=255)
tcgcAGTTTCAAGCTATTACCGCGAAACCTATGCCGAAGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCACCTGGCGGGTCATAACAATATTTTccc < 1:750593/99‑1 (MQ=255)
cgcAGTTTCAAGCTATTACCGCGAAACCTATGCCGAAGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCACCTGGCGGGTCATAACAATATTTTccca > 2:736580/1‑99 (MQ=255)
aGTTTCAAGCTATTACCGCGAAACCTATGCCGAAGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCACCTGGCGGGTCATAACAATATTTTCCCCACg < 1:172672/100‑1 (MQ=255)
gTTTCAAGCTATTACCGCGAAACCTATGCCGAAGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCACCTGGCGGGTCATAACAATATTTTCCCCACGc < 1:2447843/100‑1 (MQ=255)
ttCAAGCTATTACCGCGAAACCTATGCCGAAGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCACCTGGCGGGTCATAACAATATTTTCCCCACGCtt > 1:1078044/1‑100 (MQ=255)
ttCAAGCTATTACCGCGAAACCTATGCCGAAGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCACCTGGCGGGTCATAACAATATTTTCCCCACGCtt > 1:5304/1‑100 (MQ=255)
aaGCTATTACCGCGAAACCTATGCCGAAGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCACCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCa > 1:234321/1‑100 (MQ=255)
gCTATTACCGCGAAACCTATGCCGAAGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCACCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATg > 1:1475497/1‑100 (MQ=255)
gCTATTACCGCGAAACCTATGCCGAAGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCACCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATg < 1:1657421/100‑1 (MQ=255)
gCTATTACCGCGAAACCTATGCCGAAGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCACCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATg > 1:376162/1‑100 (MQ=255)
ttATTACCTCTAAACCTATGCCGAAGCAGAACAAAGTTTAGGTGTAGTTCGCGCCCTGCACCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATgg < 2:64512/99‑1 (MQ=255)
aTTACCGCGAAACCTATGCCGAAGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCACCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCt > 2:78413/1‑100 (MQ=255)
ttACCGCGAAACCTATGCCGAAGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCACCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTa < 2:58015/100‑1 (MQ=255)
ttACCGCGAAACCTATGCCGAAGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCACCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTa > 1:579200/1‑100 (MQ=255)
ttACCGCGAAACCTATGCCGAAGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCACCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTa < 1:327343/100‑1 (MQ=255)
ttACCGCGAAACCTATGCCGAAGCAGAACAACGTATAGGTGAAGTACCCGCCCTGCACCTGGCGTGTAATACCAATATTTGCCCCACGCTTTCATGGCTa > 2:2170584/1‑100 (MQ=255)
tACCGCGAAACCTATGCCGAAGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCACCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTaa > 2:388963/1‑100 (MQ=255)
cgcgAAACCTATGCCGAAGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCACCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACg > 1:182864/1‑100 (MQ=255)
ccTATGCCGAAGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCACCTGGCGGGTCATAACAATATTTTCCCCAAGCTTTCATGGCTAAACGGCACTGc > 2:1141227/1‑100 (MQ=255)
cTATGCCGAAGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCACCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGcc > 1:1425620/1‑100 (MQ=255)
cTATGCCGAAGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCACCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGcc < 1:1736754/100‑1 (MQ=255)
cTATGCCGAAGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCACCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGACTAAACGGCACTGcc < 2:1383240/100‑1 (MQ=255)
tATGCCGAAGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCACCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCca > 2:110155/1‑100 (MQ=255)
tATGCCGAAGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCACCTGGCGGGACATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGccc > 1:1287815/1‑99 (MQ=255)
aTGCCGAAGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCACCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCcac < 1:190977/100‑1 (MQ=255)
gCCGAAGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCACCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCacac > 2:1066074/1‑100 (MQ=255)
gCCGAAGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCACCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCacac > 2:741749/1‑100 (MQ=255)
ccGAAGCAGAACAACGTTTAGGTGAAGTTCGCGCGCTGCACCTGGCGGGTCATGACAATATTCTCCCCACGCTTGCATGGCTAAACGGCACTGCCACACt > 1:2457889/1‑100 (MQ=255)
aaGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCACCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCcg > 1:793835/1‑100 (MQ=255)
aaGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCACCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCcg < 1:1724012/100‑1 (MQ=255)
gCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCACCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCcgcg < 1:2584027/100‑1 (MQ=255)
gCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCACCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCcgcg > 2:452217/1‑100 (MQ=255)
gCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCACCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCcgcg > 1:48218/1‑100 (MQ=255)
cAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCACCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGt > 1:2607086/1‑100 (MQ=255)
cAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCACCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGt > 1:547709/1‑100 (MQ=255)
aGAACAACGTTTAGGTGAAGTTCGCGCCCTGCACCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTc > 2:1852145/1‑100 (MQ=255)
acaacGTTTAGGTGAAGTTCGCGCCCTGCGCCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTgg < 2:794834/100‑1 (MQ=255)
tAGGTGAAGTTCGCGCCCTGCACCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCcgcg < 1:17653/100‑1 (MQ=255)
tAGGTGAAGTGCGCGCCCTGTATCGGGCGGGTCATAAGAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCAGCcgcg < 2:888773/100‑1 (MQ=255)
aGGTGAAGTTCGCGCCCTGCACCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCcgcgc > 2:1220485/1‑100 (MQ=255)
ggTGAAGTTCGCGCCCTGCACCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCcgcgcg > 2:662865/1‑100 (MQ=255)
gTGAAGTTCGCGCCCTGCACCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCgg > 2:265940/1‑100 (MQ=255)
gTGAAGTTCGCGCCCTGCACCTGGCGGGTCATAACAATATTTTACCAACGCTTTCATGGCTCAACGGCCCTGCCACACTCCGCGTATGGCATCCGAGCgg > 1:2515523/1‑100 (MQ=255)
tGAAGTTCGCGCCCTGCACCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGGc > 1:326669/1‑100 (MQ=255)
gAAGTTCGCGCCCTGCACCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGGcc < 1:901365/100‑1 (MQ=255)
gAAGTTCGCGACCTGCACCTGGCGGGTCATAACAATATTTTCCACACGCTATCATGACTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGGcc > 2:1141602/1‑100 (MQ=255)
aGTTCGCGCCCTGCACCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGGCCCt > 2:727333/1‑100 (MQ=255)
aGTTCGCGCCCTGCACCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGGCCCt > 2:1191811/1‑100 (MQ=255)
aGTTCGCGCCCTGCACCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGGCCCt > 1:1700537/1‑100 (MQ=255)
gTTCGCGCCCTGCACCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGGCCCTg < 1:1499399/100‑1 (MQ=255)
cgcgCCCTGCACCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGGCCCTGATc < 1:196193/100‑1 (MQ=255)
gcgcCCTGCACCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGGCCCTGATCa < 1:1093124/100‑1 (MQ=255)
cccTTCCCCGGGCGGCGCAAAACACTAGTATCCCCACGCTTTCATGGCTAAGGGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGGCCCTGATCAAGt < 2:2119047/100‑1 (MQ=255)
ccTGCACCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGGCCCTGATCAAGtt > 2:2409717/1‑100 (MQ=255)
cTGCACCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGGCCCTGATCAAGTTg < 1:332122/100‑1 (MQ=255)
|
GGATGCTAATGTCTGGGTCGATGGCGCAGTTTCAAGCTATTACCGCGAAACCTATGCCGAAGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCGCCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGGCCCTGATCAAGTTG > NC_000913/2669841‑2670031
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
