Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F380 I0 R1
|
15 |
20.3 |
466148 |
96.4% |
449366 |
201.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
139,326 |
C→T |
100% |
G634S (GGC→AGC) |
gcd ← |
glucose dehydrogenase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 139,326 | 0 | C | T | 100.0%
| 109.6
/ NA
| 30 | G634S (GGC→AGC) | gcd | glucose dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (18/12); total (18/12) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
CACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCCTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGACCAGCGTACCCTGTTCAGATGGCGGGGTGAAAATGCCTTCATAGCGCATCTGGTGGAACATCACGCGGCACACCAGTTG > NC_000913/139117‑139560
|
cACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGt > 1:189291/1‑239 (MQ=255)
cgTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACCAACGGCa > 1:147452/1‑239 (MQ=255)
cAATCCGTTTCTTCCAACCCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGcc < 2:189291/239‑1 (MQ=255)
tcCACACTCTTTCATTAGTTTGCAGATCCAGCGCCGAGATATACCCCAATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCa < 2:147452/239‑1 (MQ=255)
cTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCACt > 1:77409/1‑239 (MQ=255)
cAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCACTTCACGATTTGGATCAACgg > 1:126413/1‑239 (MQ=255)
tGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACa > 1:31787/1‑239 (MQ=255)
ggCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACATccc < 1:136875/227‑1 (MQ=255)
ggCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACATccc > 2:136875/1‑227 (MQ=255)
agagGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGACCAGCGTAc > 1:138018/1‑237 (MQ=255)
gagGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCa > 1:162521/1‑146 (MQ=255)
gagGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCa < 2:162521/146‑1 (MQ=255)
aCGGGTTGGGCTTGACCCCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGACCAGCGTACCCTGTTc < 2:31787/238‑1 (MQ=255)
aCGGGTTGAGCGTGACCCCATCCGGTACCCCGTACGGGGGCTGAATGCCGGATTCCGTCCCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGACCAGCGTACCCTGTTc < 2:138018/238‑1 (MQ=255)
cGGTACCCCGTACTGGCGCTAAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGACCAGCGTACCCTGTTCAGATGGCGGGGTGaa < 2:48698/231‑1 (MQ=255)
cGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGACCAGCGTACCCTGTTCAGATGGCGGGGTGaa > 1:22437/1‑223 (MQ=255)
cGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGACCAGCGTACCCTGTTCAGATGGCGGGGTGaa < 2:22437/223‑1 (MQ=255)
cGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGACCAGCGTACCCTGTTCAGATGGCGGGGTGAAAATGCCTTCATAtcgc > 1:128009/1‑239 (MQ=255)
tACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCACTTCACGATTTGGAt > 1:214558/1‑142 (MQ=255)
tACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCACTTCACGATTTGGAt < 2:214558/142‑1 (MQ=255)
tACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACATCCCCAGGt > 1:231955/1‑178 (MQ=255)
tACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACATCCCCAGGt < 2:231955/178‑1 (MQ=255)
tACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGACCAGCGTACCCTGTTCAGATGGCGGGGTGAAAATGCCTTCa > 1:48698/1‑231 (MQ=255)
tgtgGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGACCAGCGTACCCTGTTCAGATGGCGGGGTGCAAATGCCTTCATAGCGCa > 1:97537/1‑235 (MQ=255)
tgtgGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGACCAGCGTACCCTGTTCAGATGGCGGGGTGAAAATGCCTTCATAGCGCa > 1:111782/1‑235 (MQ=255)
ccGTGATTTTGGACTCTTTCGGCTTATCAGTCGGGTTGCCAGGACCGACCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGACCAGCGTACCCTGTTCAGATGGCGGGGTGAAAATGCCTTCATAGCGCATCTGGTGGAACATCACGCGGCACACCAGt < 2:97537/239‑1 (MQ=255)
tGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGACCAGCGTACCCTGTTCAGATGGCGGGGTGAAAATGCCTTCATAGCGCATCTGGTGGAACATCAcg < 2:105662/224‑1 (MQ=255)
tGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGACCAGCGTACCCTGTTCAGATGGCGGGGTGAAAATGCCTTCATAGCGCATCTGGTGGAACATCAcg > 1:105662/1‑224 (MQ=255)
tGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGACCAGCGTACCCTGTTCAGATGGCGGGGTGAAAATGCCTTCATAGCGCATCTGGTGGAACATCACGCGGCACACCAGTTg > 1:167524/1‑238 (MQ=255)
tGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGACCAGCGTACCCTGTTCAGATGGCGGGGGGAAAATGCCTTCATATCGCATCTGGTGGAACa > 1:193283/1‑219 (MQ=255)
|
CACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCCTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGACCAGCGTACCCTGTTCAGATGGCGGGGTGAAAATGCCTTCATAGCGCATCTGGTGGAACATCACGCGGCACACCAGTTG > NC_000913/139117‑139560
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
