Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F380 I1 R1
|
14 |
43.2 |
1447182 |
96.3% |
1393636 |
142.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
139,326 |
C→T |
G634S (GGC→AGC) |
gcd ← |
glucose dehydrogenase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 139,326 | 0 | C | T | 97.9%
| 180.6
/ NA
| 47 | G634S (GGC→AGC) | gcd | glucose dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (22/24); total (22/25) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGT‑GACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCCTTTGG‑CATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGACCAGCGTACCCTGTTCAGATGGCGGGGTGAAAATGCCTTCATAGCGCA > NC_000913/139153‑139529
|
cggcgTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGT‑GACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGG‑CATCTTTCGGCTGCTCCATCGGGTTGCCAGGAc > 1:150446/1‑212 (MQ=255)
cggcgTACCAATACGTTTCTTCCACAACACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGT‑GACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGG‑CATCTTTCGGCTGCTCCATCGGGTTGCCAGGAc < 2:150446/212‑1 (MQ=255)
gTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGT‑GACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGG‑CATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAg < 3:153958/239‑1 (MQ=255)
tACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGT‑GACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGG‑CATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGAt < 2:153250/217‑1 (MQ=255)
tACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGT‑GACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGG‑CATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGAt > 1:153250/1‑217 (MQ=255)
tACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGT‑GACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGG‑CATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGt > 3:341431/1‑239 (MQ=255)
tACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGT‑GACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGG‑CATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCAtt < 3:141306/239‑1 (MQ=255)
ttcttcCACACGCATTCATTGGTTTTCAGATCCAGCGCCGAGATATCACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGT‑GACACCATACGGTACCCCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGG‑CATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGt < 4:341431/238‑1 (MQ=255)
ttcCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGT‑GACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGG‑CATCTTTCGGCTGCTCCATCgg < 4:117017/182‑1 (MQ=255)
ttcCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGT‑GACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGG‑CATCTTTCGGCTGCTCCATCgg > 3:117017/1‑182 (MQ=255)
cATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGT‑GACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGG‑CATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCACTTCa < 1:34381/239‑1 (MQ=255)
taACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGT‑GACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGG‑CATCTTTCGGCTGCTCCATCGGGTTGCCAGGACAACGCGGGATCAGTTTCGCAACAAACGGCAGTGCCATTGGGTTGGGAATCGTCACGTCACGATTTGGATCAACGGAAATCTCGtcc > 4:155009/1‑237 (MQ=255)
ccATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGT‑GACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGG‑CATCTTTCGGCTGCTCCATCgg < 3:230515/135‑1 (MQ=255)
ccATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGT‑GACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGG‑CATCTTTCGGCTGCTCCATCgg > 4:230515/1‑135 (MQ=255)
aTGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGT‑GACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGG‑CATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAAc > 1:95466/1‑239 (MQ=255)
gctgTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGT‑GACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGG‑CATCTTTCGGCTGCTCCATCGGGTTGCCGGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCACTTCACGATTTGGATTAACGGAAATCCCGCCCCAATCGAAAATtccc > 4:317991/1‑239 (MQ=255)
gctgTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGT‑GACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGG‑CATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCACTTCACGATTTGGATCAACGGCAATCCCGCCCCATTCGAACATcccc > 3:292323/1‑239 (MQ=255)
ttACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGT‑GACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGG‑CATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCAtt < 2:228688/171‑1 (MQ=255)
ttACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGT‑GACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGG‑CATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCAtt > 1:228688/1‑171 (MQ=255)
aCCAAATGGTGAGAGGAACGGGTTGAGCGT‑GACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGG‑CATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAg > 3:162451/1‑238 (MQ=255)
aCCAAATGGTGAGAGGAACGGGTTGAGCGT‑GACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGG‑CATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGACCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCACTTTACGATTTGGATCAACGGACATACAGCCGCATTCGTACAATCCCAGGTTACCCGGGAAgc > 2:67225/1‑238 (MQ=255)
aCCAAATGGTAGGAAGACGGGGTTGAGCGTAGACACAAT‑CGGTACACCGTACTGTGGATAAATCCCGGTCTCCGTACCCGTCCTTTTGG‑CCTCTTTCGGCTGCTCCATCGGGTTTCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGa < 4:292323/239‑1 (MQ=255)
ccAAATGGTGAGAGGAACGGGTTGAGCGT‑GACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGG‑CATCTTTCGGCTGCTCCATCGGGTTGCCGGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGa < 3:317991/238‑1 (MQ=255)
aTGGTGAGAGGAACGGGTTGAGCGT‑GACACCATACGGTACACCGTACTGTGGCTTAATGCCGGATTCCGTACCCGTGCTTTTGG‑CATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGACCAGc < 3:155009/239‑1 (MQ=255)
gagGAACGGGTTGAGCGT‑GACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGG‑CATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGACCATCCCCAGGTTAACCGGGAAGACCAGCggaa > 4:476304/1‑233 (MQ=255)
aaCGGGTTGAGCGT‑GACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGG‑CATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGACCAGCGTAccc < 3:239800/234‑1 (MQ=255)
tGAGCGT‑GACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGG‑CATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGACCAGCGTACCCTGTTCAGATgg < 3:476304/238‑1 (MQ=255)
catgtacgccgtACGGTAAGCGGGACTGGGGCTGAATGGGGGATCCGGTA‑CCGGGACTATGGACATCGTTCGGCTGCTCCAGCGGTGTGCCAGGACCACGCGGGATCAGTTGCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCACTGCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACAGCCCCAGGTTACCCGGGAAGACCAGCGTACCCTGTTCAGAggg < 4:325497/224‑1 (MQ=255)
acacCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGG‑CATCTTTCGgct < 4:355815/70‑1 (MQ=255)
acacCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGG‑CATCTTTCGgct > 3:355815/1‑70 (MQ=255)
acacCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGG‑CATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCAc > 3:290504/1‑94 (MQ=255)
acacCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGG‑CATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCAc < 4:290504/94‑1 (MQ=255)
acCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGG‑CATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCa > 3:185673/1‑107 (MQ=255)
acCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGG‑CATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCa < 4:185673/107‑1 (MQ=255)
gTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGG‑CATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGACCAGCGTACCCTGTTCAGATGGCGGGGTGAAAATGCCTTCATAGCGCa < 3:283090/239‑1 (MQ=255)
tACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGG‑CATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCa < 1:224139/103‑1 (MQ=255)
tACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGG‑CATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCa > 2:224139/1‑103 (MQ=255)
gAATGCCGGATTCCGTACCCGTGCTTTTGG‑CATCTTTCGGCTGCTCCATCgg < 3:323377/52‑1 (MQ=255)
gAATGCCGGATTCCGTACCCGTGCTTTTGG‑CATCTTTCGGCTGCTCCATCgg > 4:323377/1‑52 (MQ=255)
gAATGCCGGATTCCGTACCCGTGCTTTTGG‑CATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGACCAGCGTACCCTGTTCAGATGGCGGGGTGAAAATGCCTTCATAGCGCa > 1:5852/1‑228 (MQ=255)
gAATGCCGGATTCCGTACCCGTGCTTTTGG‑CATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGACCAGCGTACCCTGTTCAGATGGCGGGGTGAAAATGCCTTCATAGCGCa < 2:5852/228‑1 (MQ=255)
ccGTGCTTTTGG‑CATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGa > 3:318874/1‑138 (MQ=255)
ccGTGCTTTTGG‑CATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGa < 4:318874/138‑1 (MQ=255)
gTGCTTTTGG‑CATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGACCAGCGTACCCTGTTCAGATGGCGGGGTGAAAATGCCTTCATAGCGCa < 3:106574/208‑1 (MQ=255)
gTGCTTTTGG‑CATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGACCAGCGTACCCTGTTCAGATGGCGGGGTGAAAATGCCTTCATAGCGCa > 4:106574/1‑208 (MQ=255)
tGCTTTTGG‑CATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACATc > 4:328242/1‑140 (MQ=255)
tGCTTTTGG‑CATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACATc < 3:328242/140‑1 (MQ=255)
|
CGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGT‑GACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCCTTTGG‑CATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGACCAGCGTACCCTGTTCAGATGGCGGGGTGAAAATGCCTTCATAGCGCA > NC_000913/139153‑139529
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
