Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F406 I1 R1 19 66.1 1879446 94.7% 1779835 168.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,330,404 A→C pseudogene (3999/4605 nt) yfaS ← pseudogene, bacterial alpha2‑macroglobulin YfaS variant family; putative membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,330,4040AC98.6% 263.3 / NA 70pseudogene (3999/4605 nt)yfaSpseudogene, bacterial alpha2‑macroglobulin YfaS variant family; putative membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base C (33/36);  minor base T (0/1);  total (33/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCGGTTGCAGAATAAAGCTCATCTCTTCTTCACCAGGGATAAGCCGATACAACTGGCGTTCAACGGTCACCGGAATGTTACTTTGTTGAGCCATTTTTGCCGGCTCACGTCAGCGGACCTGCACATTTTGCGGCGATAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAG‑TTAACTTATGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTCAGCGGTCAAACCACTTAAAATAGCGGCCGTCTGGGTAGCGTCGCCACCGCTATTAAGCAGCACAACCGTGTGCGCCAGCGGCTGATTGCTGTTTGCCGCTTTATCCCACGCATATT  >  NC_000913/2330189‑2330627
                                                                                                                                                                                                                          |                                                                                                                                                                                                                               
aCCGGTTGCAGAATAAAGCTCATCTCTTCTTCACCAGGGATAAGCCGATACAACTGGCGTTCAACGGTCACCGGAATGTTACTTTGTTGAGCCATTTTTGCCGGCTCACGTCAGCGGACCTGCACATTTTGCGGCGATAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAG‑TTAACTTCTGTTTAGCCCATGCGCCcgcag                                                                                                                                                                                                           >  2:835815/1‑238 (MQ=255)
  ctgTTTCAGAAAACAGCTCTTCTCAGCTGCACGAGAGATAGGCCGATACAACTGGCCTGCAACGGTCCCCTGAATGTGTCTTTGTTGATCCATTTTTGCCGGCGCACGTCAGGGGACCTGCACATTGTGCGGCGATAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAGCGCCAGTCATCGCCCCCTCCAG‑TTAACATCTGTTTAGCCCAAGCGCCcgcaggcg                                                                                                                                                                                                        <  2:883877/237‑1 (MQ=255)
  cGGTTGCAGAATAAAGCTCATCTCTTCTTCACCAGGGATAAGCCGATACAACTGGCGTTCAACGGTCACCGGAATGTTACTTTGTTGAGCCATTTTTGCCGGCTCACGTCAGCGGACCTGCACATTTTGCGGCGATAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAG‑TTAACTTCTGTTTAGCCCATGCGCCcgcaggcg                                                                                                                                                                                                        <  2:651363/239‑1 (MQ=255)
                     aTCTCTTCTTCACCAGGGATAAGCCGATACAACTGGCGTTCAACGGTCACCGGAATGTTACTTTGTTGAGCCATTTTTGCCGGCTCACGTCAGCGGACCTGCACATTTTGCGGCGATAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAG‑TTAACTTCTGCTTAGCCCATGCGCCCGCAGGCGCAGGCCACACAAATGGaag                                                                                                                                                                                     >  2:752258/1‑237 (MQ=255)
                       ctctTCTTCACCAGGGATAAGCCGATACAACTGGCGTTCAACGGTCACCGGAATGTTACTTTGTTGAGCCATTTTTGCCGGCTCACGTCAGCGGACCTGCACATTTTGCGGCGATAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAG‑TTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCa                                                                                                                                                                                   >  1:226938/1‑239 (MQ=255)
                       ctctTCTTCACCAGGGATAAGCCGATACAACTGGCGTTCAACGGTCACCGGAATGTTACTTTGTTGAGCCATTTTTGCCGGCTCACGTCAGCGGACCTGCACATTTTGCGGCGATAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAG‑TTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCa                                                                                                                                                                                   >  1:60262/1‑239 (MQ=255)
                          ttcttcACCAGGGATAAGCCGATACAACTGGCGTTCAACGGTCACCGGAATGTTACTTTGTTGAGCCATTTTTGCCGGCTCACGTCAGCGGACCTGCACATTTTGCGGCGATAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAG‑TTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTg                                                                                                                                                                                <  1:835815/239‑1 (MQ=255)
                                aCCAGGGATAAGCCGATTCCACTGGCGTTCAACGGTCCCCGGAATGTTACTTTGTTGAGCCATTTTTGCCGGCTCACGTCAGCGGACCTGCACATTTTGCGGCGATAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAG‑TTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCa                                                                                                                                                                          <  2:60262/239‑1 (MQ=255)
                                               aTACAACTGGCGTTCAACGGTCACCGGAATGTTACTTTGTTGAGCCATTTTTGCCGGCTCACGTCAGCGGACCTGCACATTTTGCGGCGATAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAG‑TTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCgccagcc                                                                                                                                                           <  2:226938/239‑1 (MQ=255)
                                                               aCGGTCACCGGAATGTTACTTTGTTGAGCCATTTTTGCCGGCTCACGTCAGCGGACCTGCACATTTTGCGGCGATAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAG‑TTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAgcgc                                                                                                                                           <  1:592602/239‑1 (MQ=255)
                                                                  gTCACCGGAATGTTACTTTGTTGAGCCATTTTTGCCGGCTCACGTCAGCGGACCTGCACATTTTGCGGCGATAATTCGTCCCCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGCCCAACCCAACGCCAGTCTTCGCCCCCTCCAG‑TTAACTTCTGTTTAGCCCCTGCGCCCGCATGCGCAGGCAAACCACCTGGAGGCATTGTCGCCGTATCTTTTGCCAGCCAGTTCCTGGCCAtcgctc                                                                                                                                         >  2:551955/1‑236 (MQ=255)
                                                                     aCCGGAATGTTACTTTGTTGAGCCATTTTTGCCGGCTCACGTCAGCGGACCTGCACATTTTGCGGCGATAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAG‑TTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTc                                                                                                                                     >  1:552079/1‑239 (MQ=255)
                                                                     aCCGGAATGTTACTTTGTTGAGCCATTTTTGCCGGCTCACGTCAGCGGACCTGCACATTTTGCGGCGATAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAG‑TTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTc                                                                                                                                     >  1:667212/1‑239 (MQ=255)
                                                                         gAATGTTACTTTGTTGAGCCATTTTTGCCGGCTCACGTCAGCGGACCTGCACATTTTGCGGCGATAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAG‑TTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATa                                                                                                                                 <  1:180736/239‑1 (MQ=255)
                                                                                 cTTTGTTGAGCCATTTTTGCCGGCTCACGTCAGCGGACCTGCACATTTTGCGGCGATAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAG‑TTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTg                                                                                                                                                                                >  2:628816/1‑184 (MQ=255)
                                                                                 cTTTGTTGAGCCATTTTTGCCGGCTCACGTCAGCGGACCTGCACATTTTGCGGCGATAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAG‑TTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTg                                                                                                                                                                                <  1:628816/184‑1 (MQ=255)
                                                                                 cTTTGTTGAGCCATTTTTGCCGGCTCACGTCAGCGGACCTGCACATTTTGCGGCGATAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAG‑TTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAgcgcgcg                                                                                                                                        <  2:750840/224‑1 (MQ=255)
                                                                                 cTTTGTTGAGCCATTTTTGCCGGCTCACGTCAGCGGACCTGCACATTTTGCGGCGATAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAG‑TTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAgcgcgcg                                                                                                                                        >  1:750840/1‑224 (MQ=255)
                                                                                    tgttgAGCCATTTTTGCCGGCTCACGTCAGCGGACCTGCACATTTTGCGGCGATAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAG‑TTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTc                                                                                                                      >  1:829022/1‑239 (MQ=255)
                                                                                           ccATTTTTGCCGGCTCACGTCAGCGGACCTGCACATTTTGCGGCGATAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAG‑TTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTCAGCGGTc                                                                                                               >  1:211364/1‑239 (MQ=255)
                                                                                              tttttGCCGGCTCACGTCAGCGGACCTGCACATTTTGCGGCGATAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAG‑TTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTCAGCGGTCaaa                                                                                                            <  1:693802/239‑1 (MQ=255)
                                                                                                        cTCACGTCAGCGGACCTGCACATTTTGCGGCGATAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAG‑TTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGTGCGCGCTCAATAGTGGATTGCTCAGCGGTCaaa                                                                                                            >  2:102448/1‑229 (MQ=255)
                                                                                                        cTCACGTCAGCGGACCTGCACATTTTGCGGCGATAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAG‑TTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGTGCGCGCTCAATAGTGGATTGCTCAGCGGTCaaa                                                                                                            <  1:102448/229‑1 (MQ=255)
                                                                                                             gTCAGCGGACCTGCACATTTTGCGGCGATAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAG‑TTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTCAGCGGTCAAACCACTTAAAATAGCg                                                                                             <  1:232081/239‑1 (MQ=255)
                                                                                                                  cGGACCTGCACATTTTGCGGCGATAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAG‑TTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCgccagc                                                                                                                                                            >  2:201544/1‑171 (MQ=255)
                                                                                                                  cGGACCTGCACATTTTGCGGCGATAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAG‑TTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCgccagc                                                                                                                                                            <  1:201544/171‑1 (MQ=255)
                                                                                                                   ggACCTGCACATTTTGCGGCGATAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAG‑TTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTCAGCGGTCAAACCACTTAAAATAGCGGc                                                                                           <  1:752258/235‑1 (MQ=255)
                                                                                                                   ggACCTGCACATTTTGCGGCGATAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAG‑TTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTCAGCGGTCAAACCACTTAAAATAGCGGc                                                                                           <  1:174152/235‑1 (MQ=255)
                                                                                                                     aCCTGCACATTTTGCGGCGATAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAG‑TTAACTTCTGTTTAGCCCATGCGCCcgcaggc                                                                                                                                                                                                         <  1:519261/123‑1 (MQ=255)
                                                                                                                     aCCTGCACATTTTGCGGCGATAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAG‑TTAACTTCTGTTTAGCCCATGCGCCcgcaggc                                                                                                                                                                                                         >  2:519261/1‑123 (MQ=255)
                                                                                                                       cTGCACATTTTGCGGCGATAATTCGTCACCAAAAGAGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAG‑TTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTTGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGGGGATTGCTCAGCGGTCAAACCACTTAAAATAGCGGCCg                                                                                         >  1:559354/1‑235 (MQ=255)
                                                                                                                       cTGCACATTTTGCGGCGATAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAG‑TTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTCAGCGGTCaaa                                                                                                            <  2:401007/214‑1 (MQ=255)
                                                                                                                       cTGCACATTTTGCGGCGATAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAG‑TTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTCAGCGGTCaaa                                                                                                            >  1:401007/1‑214 (MQ=255)
                                                                                                                                    ggcgATAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAG‑TTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTCAGCGGTCAAACCACTTAAAATAGCGGCCGTCTGGGTAGCGTCGCCAc                                                                       <  2:667212/238‑1 (MQ=255)
                                                                                                                                    ggcgAGAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAG‑TTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCGTATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTCAGCGGTCAAACCACTTAAAATAGCGGCCGGCTGGGTAGCGTCGCCAc                                                                       <  1:551955/238‑1 (MQ=255)
                                                                                                                                    ggccATAATTCGGACCCAAA‑‑AGGGCAAATGCCCGGCCAGCCCGGACCAACCCAACGCCAGTCTTCGCCCCCTCCAG‑TTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTCAGCGGTCAAACCACTTAAAATAGCGGCCGTCTGGGTAGCGTCGCCAc                                                                       <  2:211364/238‑1 (MQ=255)
                                                                                                                                                tCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAG‑TTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCgccagcc                                                                                                                                                           <  1:244942/142‑1 (MQ=255)
                                                                                                                                                tCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAG‑TTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCgccagcc                                                                                                                                                           >  2:244942/1‑142 (MQ=255)
                                                                                                                                                  gccAAA‑‑AGAGACAAGGTCCGGCACGCCCTGACCACCCCAACGCCATTCTTCGCCCCCTCCAG‑TTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTCAGCGGTCAAACCACTTAAAATAGCGGCCGTCTGGGTAGCGTCGCCACCGCTATTAAgcagca                                                        <  2:407586/238‑1 (MQ=255)
                                                                                                                                                       a‑‑aGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAG‑TTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTCAGCGGTCAAACCACTTAAAATAGCGGCCGTCTGGGTAGCGTCGCCACCGCTATTAag                                                             >  1:871738/1‑229 (MQ=255)
                                                                                                                                                       a‑‑aGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAG‑TTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTCAACGGTCAAACCACTTAAAATAGCGGCCGTCTGGGTAGCGTCGCCACCGCTATTAag                                                             <  2:871738/229‑1 (MQ=255)
                                                                                                                                                                aaTGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAG‑TTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAgcgcgcg                                                                                                                                        <  2:722096/147‑1 (MQ=255)
                                                                                                                                                                aaTGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAG‑TTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAgcgcgcg                                                                                                                                        >  1:722096/1‑147 (MQ=255)
                                                                                                                                                                aaTGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAG‑TTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTCAGCGGTCAAACCACTTAAAATATCGGCCGTCTGGGTAGCGTCGCCACCGCAATTAAGCAGATCAACCGTGTgc                                             >  2:926893/1‑238 (MQ=255)
                                                                                                                                                                    tCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAG‑TTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGa                                                                                                                            >  1:205446/1‑155 (MQ=255)
                                                                                                                                                                    tCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAG‑TTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGa                                                                                                                            <  2:205446/155‑1 (MQ=255)
                                                                                                                                                                      cGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAG‑TTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAg                                                                                                                                                                                      <  1:555479/95‑1 (MQ=255)
                                                                                                                                                                      cGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAG‑TTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAg                                                                                                                                                                                      >  2:555479/1‑95 (MQ=255)
                                                                                                                                                                                 gACCAACCCAACGCCAGTCTTCGCCCCCTCCAG‑TTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAgcgcgcg                                                                                                                                        >  2:215959/1‑130 (MQ=255)
                                                                                                                                                                                 gACCAACCCAACGCCAGTCTTCGCCCCCTCCAG‑TTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAgcgcgcg                                                                                                                                        <  1:215959/130‑1 (MQ=255)
                                                                                                                                                                                     aacccaacGCCAGTCTTCGCCCCCTCCAG‑TTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTCAGCGGTCAAACCACTTAAAATAGCGGCCGTCTGGGTAGCGTCGCCACCGCTATTAAGCAGCACAACCGTGTGCGCCAGCGGCTGATTGCTGTTTg                       <  1:926893/239‑1 (MQ=255)
                                                                                                                                                                                     aacccaacGCCAGCCCTCGCCCCCTCCAG‑TTAACTTTTGTTTACCCAATGCGCCCGAAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTCAGCGGTCAAACCACTTAAAATAGCGGCCGTCTGGGTAGCGTCGCCACCGCTATTAAGCAGCACAACCGTGTGCGCCAGCGGCTGATTGCTGTTTg                       <  2:281352/239‑1 (MQ=255)
                                                                                                                                                                                       cccaacGCCAGTCTTCGCCCCCTCCAG‑TTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCt                                                                                                                                      <  2:506118/126‑1 (MQ=255)
                                                                                                                                                                                       cccaacGCCAGTCTTCGCCCCCTCCAG‑TTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCt                                                                                                                                      >  1:506118/1‑126 (MQ=255)
                                                                                                                                                                                       cccaacGCCAGTCTTCGCCCCCTCCAG‑TTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTCAGCGGTCAAACCACTTAAAATAGCGGCCGt                                                                                        >  2:436552/1‑172 (MQ=255)
                                                                                                                                                                                       cccaacGCCAGTCTTCGCCCCCTCCAG‑TTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTCAGCGGTCAAACCACTTAAAATAGCGGCCGt                                                                                        <  1:436552/172‑1 (MQ=255)
                                                                                                                                                                                            cGCCAGTCTTCGCCCCCTCCAG‑TTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTCAGCGGTCAAACCACTTAAAATAGCGGCCGTCTGGGTAGCGTCGCCACCGCTATTAAGCAGCACAACCGTGTGCGCCAGCGGCTGATTGCTGATTGCCGCttt                >  1:182437/1‑239 (MQ=255)
                                                                                                                                                                                              ccAGTCTTCGCCCCCTCCAG‑TTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTCAGCGGTCAAACCACTTAAAATAGCGGCCGTCTGGGTAGCGTCGCCACCGCTATTAAGCAGCACAACCGTGTGCGCCAGCGGCTGATTGCTGTTTGCCGCTTTAt              <  2:829022/239‑1 (MQ=255)
                                                                                                                                                                                                 gTCTTCGCCCCCTCCAG‑TTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTCAGCGGTCAAACCACTTAAAATAGCGGCCGTCTGGGTAGCGTCGCCACCGCTa                                                                  >  2:230914/1‑184 (MQ=255)
                                                                                                                                                                                                 gTCTTCGCCCCCTCCAG‑TTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTCAGCGGTCAAACCACTTAAAATAGCGGCCGTCTGGGTAGCGTCGCCACCGCTa                                                                  <  1:230914/184‑1 (MQ=255)
                                                                                                                                                                                                 gTCTTCGCCCCCTCCAG‑TTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTCAGCGGTCAAACCACTTAAAATAGCGGCCGTCTGGGTAGCGTCGCCACCGCTATTa                                                               <  2:215189/187‑1 (MQ=255)
                                                                                                                                                                                                 gTCTTCGCCCCCTCCAG‑TTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTCAGCGGTCAAACCACTTAAAATAGCGGCCGTCTGGGTAGCGTCGCCACCGCTATTa                                                               >  1:215189/1‑187 (MQ=255)
                                                                                                                                                                                                  tCTTCGCCCCCTCCAG‑TTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTCAGCGGTCAAACCACTTAAAATATCGGCCGTCTGGGTAGCGTCGCCACCGCTATTAAGCAGCACAACCGTGTGCGCCAGCGGCTGATTGCTGTTTGCCGCTTTATctc           >  2:448652/1‑236 (MQ=255)
                                                                                                                                                                                                      cGCCCCCTCCAG‑TTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTCAGCGGTCAAACCACTTAAAATAGCGGCCGTCTGGGTAGCGTCGCCACCGCTATTAAACAGCCAAACCTGGTGCGCCAGCGGCTGATTGCTGTTTTCTGCTTTATCCCACGCa      >  2:409003/1‑239 (MQ=255)
                                                                                                                                                                                                       ccccccTTCAGATTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTCAGCGGTCAAACCACTTAAAATAGCGGCCGTCTGGGTAGCGTCGCCACCGCTATTAAGCAGCACAACCGTGTGCGCCAGCGGCTGATTGCTGTTTGCCGCTTTATCCCACGCa      <  2:182437/238‑1 (MQ=255)
                                                                                                                                                                                                            cTCCAG‑TTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTCa                                                                                                                     >  2:350864/1‑122 (MQ=255)
                                                                                                                                                                                                            cTCCAG‑TTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTCa                                                                                                                     <  1:350864/122‑1 (MQ=255)
                                                                                                                                                                                                            cTCCAG‑TTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTCAGCGGTCAAACCACTTAAAATAGCg                                                                                             <  2:292353/146‑1 (MQ=255)
                                                                                                                                                                                                            cTCCAG‑TTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTCAGCGGTCAAACCACTTAAAATAGCg                                                                                             >  1:292353/1‑146 (MQ=255)
                                                                                                                                                                                                            cTCCAG‑TTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTCAGCGGTCAAACCACTTAAAATAGCGGCCGTCTGGGGAGCGTCGCCACCGCTATTAAGCAGCACAACCGTGGGCGCCCGCGGCCGATTGCTGGTTGCCGCTTTATCCCACGccactt  >  2:74875/1‑232 (MQ=255)
                                                                                                                                                                                                                          |                                                                                                                                                                                                                               
ACCGGTTGCAGAATAAAGCTCATCTCTTCTTCACCAGGGATAAGCCGATACAACTGGCGTTCAACGGTCACCGGAATGTTACTTTGTTGAGCCATTTTTGCCGGCTCACGTCAGCGGACCTGCACATTTTGCGGCGATAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAG‑TTAACTTATGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTCAGCGGTCAAACCACTTAAAATAGCGGCCGTCTGGGTAGCGTCGCCACCGCTATTAAGCAGCACAACCGTGTGCGCCAGCGGCTGATTGCTGTTTGCCGCTTTATCCCACGCATATT  >  NC_000913/2330189‑2330627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A