Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I51 R1 223 69.9 1534784 95.1% 1459579 214.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,110,741 A→C F93L (TTT→TTG)  rfbA ← glucose‑1‑phosphate thymidylyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,110,7410AC96.2% 178.5 / ‑2.0 53F93L (TTT→TTG) rfbAglucose‑1‑phosphate thymidylyltransferase
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base C (21/30);  total (22/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.36e-01

CTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCAAACTCAACGACACCATAGCGTTCTGGATCATTAACGTGATAGGCAAATACCGTTGCACCACTTTCTTTGTTAACAGCGGCCTCCATTAGCTTCGGCAGATCGTGACCGTAAAAGATATTATCACCAAGAACCAAAGCACAATCATCACCACCAATAAACTCTTCACCGATGATAAATGCCTGCGCGAGGCCATCTGGGCTAGGTTGCACTTTGTACTGAAGATTCAGGCCCCACTGGCTACCGTCACCCAGCAATTGTTGAAAACGAGGAGTATCCTGAGGTGTACTGATAATCAAAATATCGCGAATACCCGCCAACATCAGTGTAGAGAGCGGGTAATAGATCATCGGTTTATCATAAATAGGTAATAGCTGTTTACTGACAGCCATAGTCACA  >  NC_000913/2110516‑2110963
                                                                                                                                                                                                                                 |                                                                                                                                                                                                                              
ctTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCAAACTCAACGACACCATAGCGTTCTGGATCATTAACGTGATAGGCAAATACCGTTGCACCACTTTCTTTGTTAACAGCGGCCTCCATTAGCTTCGGCAGATCGTGACCGTAAAAGATATTATCACCAAGAACCAAAGCACAATCATCACCACCAATAAACTCTTCACCGATGATCAATGACTgcg                                                                                                                                                                                                                      >  2:465704/1‑236 (MQ=255)
ctTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCAAACTCAACGACACCATAGCGTTCTGGATCATTAACGTGATAGGCAAATACCGTTGCACCACTTTCTTTGTTAACAGCGGCCTCCATTAGCTTCGGCAGATCGTGACCGTAAAAGATATTATCACCAAGAACCAAAGCACAATCATCACCACCAATAAACTCTTCACCGATGATAAATGCCTgcg                                                                                                                                                                                                                      >  2:36490/1‑236 (MQ=255)
   tGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCAAACTCAACGACACCATAGCGTTCTGGATCATTAACGTGATAGGCAAATACCGTTGCACCACTTTCTTTGTTAACAGCGGCCTCCATTAGCTTCGGCAGATCGTGACCGTAAAAGATATTATCACCAAGAACCAAAGCACAATCATCACCACCAATAAACTCTTCACCGATGATCAATGCCTGcgcg                                                                                                                                                                                                                    >  1:694020/1‑235 (MQ=255)
          aaCGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCAAACTCAACGACACCATAGCGTTCTGGATCATTAACGTGATAGGCAAATACCGTTGCACCACTTTCTTTGTTAACAGCGGCCTCCATTAGCTTCGGCAGATCGTGACCGTAAAAGATATTATCACCAAGAACCAAAGCACAATCATCACCACCAATAAACTCTTCACCGATGATCAATGCCTGCGCGAGGCCAt                                                                                                                                                                                                             <  1:423416/235‑1 (MQ=255)
              gTTTTTCTTCCAGACTGATTGCCTTACCTTTTTTAGCAAACTCAACGACACCATAGCTTTCTGGATCATTAACGTGATAGGCAAATACCGTTGCACCACTTTCTTTGTTAACAGCGGCCTCCATTAGCTTCGGCAGATCGTGACCGTAAAAGATATTATCACCAAGAACCAAAGCACAATCATCACCACCAATAAACTCTTCACCGATGATCAATGCCTGCGCGAGGCCATCTggg                                                                                                                                                                                                        <  2:383672/236‑1 (MQ=255)
                     ttcCAGACTGATTGCCTTACCGTTTTTATCAAACTCAACGACACCATAGCGTTCTGGATCATTAACGTGATAGGCAAATACCGTTGCACCACTTTCTTTGTTAACAGCGGCCTCCATTAGCTTCGGCAGATCGTGACCGTAAAAGATATTATCACCAAGAACCAAAGCACAATCATCACCACCAATAAACTCTTCACCGATGATCAATGCCTGCGCGAGGCCATCTGGGCTAGGtt                                                                                                                                                                                                 <  1:401609/236‑1 (MQ=255)
                        catgcTGATTGCCGTACCGGTTTTAGCAATCTCAACGACCCCATAGCGTTCTGGATCATTAACGTGATAGGCAAATACCGTTGCACCACTTTCTTTGTTAACAGCGGCCTCCATTAGCTTCGGCAGATCGTGACCGTAAAAGATATTATCACCAAGAACCAAAGCACAATCATCACCACCAATAAACTCTTCACCGATGATCAATGCCTGCGCGAGGCCATCTGGGCTAGGTTGCa                                                                                                                                                                                              <  2:270927/232‑1 (MQ=255)
                         aGACTGATTGCCGTACCGTTTTGATCAAACTCAACGACTCCATAGCGTTCTGGATCATTAACGTGATAGGCAAATACCGTTGCACCACTTTCTTTGTTAACAGCGGCCTCCATTAGCTTCGGCAGATCGTGACCGTAAAAGATATTATCACCAAGAACCAAAGCACAATCATCACCACCAATAAACTCTTCACCGATGATCAATGCCTGCGCGAGGCCATCCGCGCAAGGtctggc                                                                                                                                                                                             <  2:636627/236‑6 (MQ=255)
                                      tACCGTTTTTATCAAACTCAACGACACCATAGCGTTCTGGATCATTAACGTGATAGGCAAATACCGTTGCACCACTTTCTTTGTTAACAGCGGCCTCCATTAGCTTCGGCAGATCGTGACCGTAAAAGATATTATCACCAAGAACCAAAGCACAATCATCACCACCAATAAACTCTTCACCGATGATCAATGCCTGCGCGAGGCCATCTGGGCTAGGTTGCACTTTGTACTGAAGa                                                                                                                                                                                >  1:613459/1‑236 (MQ=255)
                                        ccGTTTTTATCAAACTCAACGACACCATAGCGTTCTGGATCATTAACGTGATAGGCAAATACCGTTGCACCACTTTCTTTGTTAACAGCGGCCTCCATTAGCTTCGGCAGATCGTGACCGTAAAAGATATTATCACCAAGAACCAAAGCACAATCATCACCACCAATAAACTCTTCACCGATGATCAATGCCTGCGCGAGGCCATCTGGGCTAGGTTGCACTTTGTACTGAAGAtt                                                                                                                                                                              <  2:486391/236‑1 (MQ=255)
                                          gTTTTTATCAAACTCAACGACACCATAGCGTTCTGGATCATTAACGTGATAGGCAAATACCGTTGCACCACTTTCTTTGTTAACAGCGGCCTCCATTAGCTTCGGCAGATCGTGACCGTAAAAGATATTATCACCAAGAACCAAAGCACAATCATCACCACCAATAAACTCTTCACCGATGATAAATGCCTGCGCGAGGCCATCTGGGCTAGGTTGCACTTTGTACTGAAGATTCa                                                                                                                                                                            <  1:36490/236‑1 (MQ=255)
                                            ttttATCAAACTCAACGACACCATAGCGTTCTGGATCATTAACGTGATAGGCAAATACCGTTGCACCACTTTCTTTGTTAACAGCGGCCTCCATTAGCTTCGGCAGATCGTGACCGTAAAAGATATTATCACCAAGAACCAAAGCACAATCATCACCACCAATAAACTCTTCACCGATGATCAATGCCTGCGCGAGGCCATCTGGGCTAGGTTGCACTTTGTACTGAAGATTCAgg                                                                                                                                                                          <  1:715047/236‑1 (MQ=255)
                                                 tCAAACTCAACGACACCATAGCGTTCTGGATCATTAACGTGATAGGCAAATACCGTTGCACCACTTTCTTTGTTAACAGCGGCCTCCATTAGCTTCGGCAGATCGTGACCGTAAAAGATATTATCACCAAGAACCAAAGCACAATCATCACCACCAATAAACTCTTCACCGATGATCAATGCCTGCGCGAGGCCATCTGGGCTAGGTTGCACTTTGTACTGAAGATTCAGGCCCCa                                                                                                                                                                     <  2:733835/236‑1 (MQ=255)
                                                 tCAAACTCAACGACACCATAGCGTTCTGGATCATTAACGTGATAGGCAAATACCGTTGCACCACTTTCTTTGTTAACAGCGGCCTCCATTAGCTTCGGCAGATCGTGACCGTAAAAGATATTATCACCAAGAACCAAAGCACAATCATCACCACCAATAAACTCTTCACCGATGATCAATACCTGCGCGAGGCCATCTGGGCTAGGTTGCACTTTGTACTGAAGATTCAGGCCCCa                                                                                                                                                                     <  2:694020/236‑1 (MQ=255)
                                                         aaCGACACCATAGCGTTCTGGATCATTAACGTGATAGGCAAATACCGTTGCACCACTTTCTTTGTTAACAGCGGCCTCCATTAGCTTCGGCAGATCGTGACCGTAAAAGATATTATCACCAAGAACCAAAGCACAATCATCACCACCAATAAACTCTTCACCGATGATCAATGCCTGCGCGAGGCCATCTGGGCTAGGTTGCACTTTGTACTGAAGATTCAGGCCCCACTg                                                                                                                                                                  >  2:425263/1‑231 (MQ=255)
                                                              cacCATAGCGTTCTGGATCATTAACGTGATAGGCAAATACCGTTGCACCACTTTCTTTGTTAACAGCGGCCTCCATTAGCTTCGGCAGATCGTGACCGTAAAAGATATTATCACCAAGAACCAAAGCACAATCATCACCACCAATAAACTCTTCACCGATGATCAATGCCTGCGCGAGGCCATCTGGGCTAGGTTGCACTTTGTACTGAAGATTCAGGCCCCACTGGCTACCGTCa                                                                                                                                                        <  1:345495/236‑1 (MQ=255)
                                                                   tAGCGTTCTGGATCATTAACGTGATAGGCAAATACCGTTGCACCACTTTCTTTGTTAACAGCGGCCTCCATTAGCTTCGGCAGATCGTGACCGTAAAAGATATTATCACCAAGAACCAAAGCACAATCATCACCACCAATAAACTCTTCACCGATGATCAATGCCTGCGCGAGGCCATCTGGGCTAGGTTGCACTTTGTACTGAAGATTCAGGCCCCACTg                                                                                                                                                                  <  1:582327/221‑1 (MQ=255)
                                                                   tAGCGTTCTGGATCATTAACGTGATAGGCAAATACCGTTGCACCACTTTCTTTGTTAACAGCGGCCTCCATTAGCTTCGGCAGATCGTGACCGTAAAAGATATTATCACCAAGAACCAAAGCACAATCATCACCACCAATAAACTCTTCACCGATGATCAATGCCTGCGCGAGGCCATCTGGGCTAGGTTGCACTTTGTACTGAAGATTCAGGCCCCACTg                                                                                                                                                                  >  2:582327/1‑221 (MQ=255)
                                                                                  ttAACGTGATAGGCAAATACCGTTGCACCACTTTCTTTGTTAACAGCGGCCTCCATTAGCTTCGGCAGATCGTGACCGTAAAAGATATTATCACCAAGAACCAAAGCACAATCATCACCACCAATAAACTCTTCACCGATGATCAATGCCTGCGCGAGGCCATCTGGGCTAGGTTGCACTTTGTACTGAAGATTCAGGCCCCACTGGCTACCGTCACCCAGCAATTGTTGAAAACg                                                                                                                                    >  2:673295/1‑236 (MQ=255)
                                                                                  ttAACGTGATAGGCAAATACCGTTGCACCACTTTCTTTGTTAACAGCGGCCTCCATTAGCTTCGGCAGATCGTGACCGTAAAAGATATTATCACCAAGAACCAAAGCACAATCATCACCACCAATAAACTCTTCACCGATGATCAATGCCTGCGCGAGGCCATCTGGGCTAGGTTGCACTTTGTACTGAAGATTCAGGCCCAACTGGCTACCGTCACCCAGCAATTGTTTAAAAct                                                                                                                                    >  2:647743/1‑235 (MQ=255)
                                                                                       gTGATAGGCAAATACCGTTGCACCACTTTCTTTGTTAACAGCGGCCTCCATTAGCTTCGGCAGATCGTGACCGTAAAAGATATTATCACCAAGAACCAAAGCACAATCATCACCACCAATAAACTCTTCACCGATGATCAATGCCTGCGCGAGGCCATCTGGGCTAGGTTGCACTTTGTACTGAAGATTCAGGCCCCACTGGCTACCGTCACCCAGCAATTGTTGAAAACgaggag                                                                                                                               <  1:4856/236‑1 (MQ=255)
                                                                                          aTAGGCAAATACCGTTGCACCACTTTCTTTGTTAACAGCGGCCTCCATTAGCTTCGGCAGATCGTGACCGTAAAAGATATTATCACCAAGAACCAAAGCACAATCATCACCACCAATAAACTCTTCACCGATGATCAATGCCTGCGCGAGGCCATCTGGGCTAGGTTGCACTTTGTACTGAAGAt                                                                                                                                                                               <  1:53077/185‑1 (MQ=255)
                                                                                          aTAGGCAAATACCGTTGCACCACTTTCTTTGTTAACAGCGGCCTCCATTAGCTTCGGCAGATCGTGACCGTAAAAGATATTATCACCAAGAACCAAAGCACAATCATCACCACCAATAAACTCTTCACCGATGATCAATGCCTGCGCGAGGCCATCTGGGCTAGGTTGCACTTTGTACTGAAGAt                                                                                                                                                                               >  2:53077/1‑185 (MQ=255)
                                                                                          aTAGGCAAATACCGTTGCACCACTTTCTTTGTTAACAGCGGCCTCCATTAGCTTCGGCAGATCGTGACCGTAAAAGATATTATCACCAAGAACCAAAGCACAATCATCACCACCAATAAACTCTTCACCGATGATCAATGCCTGCGCGAGGCCATCTGGGCTAGGTTGCACTTTGTACTGAAGATTCAGGCCCCACTGGCTACCGTCACCCAGCAATTGTTGAAAACgaggag                                                                                                                               >  1:567822/1‑233 (MQ=255)
                                                                                                  aTACCGTTGCACCACTTTCTTTGTTAACAGCGGCCTCCATTAGCTTCGGCAGATCGTGACCGTAAAAGATATTATCACCAAGAACCAAAGCACAATCATCACCACCAATAAACTCTTCACCGATGATCAATGCCTGCGCGAGGCCATCTGGGCTAGGTTGCACTTTGTACTGAAGATTCAGGCCCCACTGGCTACCGTCACCCAGCAATTGTTGAAAACGAGGAGTATCCTg                                                                                                                        <  1:425263/232‑1 (MQ=255)
                                                                                                        ttGCACCACTTTCTTTGTTAACAGCGGCCTCCATTAGCTTCGGCAGATCGTGACCGTAAAAGATATTATCACCAAGAACCAAAGCACAATCATCACCACCAATAAACTCTTCACCGATGATCAATGCCTGCGCGAGGCCATCTGGGCTAGGTTGCACTTTGTACTGAAGATTCAGGCCCCACTGGCTACCGTCACCCAGCAATTGTTGAAAACGAGGAGTATCCTGAGGTGTACTg                                                                                                              >  1:676759/1‑236 (MQ=255)
                                                                                                            tccacTTTCATGGTTAACAGCGGCCTCCATTAGCTTCGGCAGATCGTGACCGTAAAAGATATTATCACCAAGAACCAAAGCACAATCATCACCACCAATAAACTCTTCACCGATGATCAATGCCTGCGCGAGGCCATCTGGGCTAGGTTGCACTTTGTACTGAAGATTCAGGCCCCACTGGCTACCGTCACCCAGCAATTGTTGAAAACGAGGAGTATCCTGAGGTGTACTg                                                                                                              <  2:676759/231‑1 (MQ=255)
                                                                                                                ctttctttGTTAACAGCGGCCTCCATTAGCTTCGGCAGATCGTGACCGTAAAAGATATTATCACCAAGAACCAAAGCACAATCATCACCACCAATAAACTCTTCACCGATGATCAATGCCTGCGCGAGGCCATCTGGGCTAGGTTGCACTTTGTACTGAAGATTCAGGCCCCACTGGCTACCGTCACCCAGCAATTGTTGAAAACGAGGAGTATCCTGAGGTGTACTGATAATCaa                                                                                                      >  1:592172/1‑236 (MQ=255)
                                                                                                                             cagcagACTCCATTAACTTCGGCAGATCGTGACCGTAAAAGATATTATCACCAAGAACCAAAGCACATTCATCACCACCAATAAACTCTTCACCGATGATCAATGCCTGCGCGAGGCCATCTGGGCTAGGTTGCACTTTGTACTGAAGATTCAGGCCCCACTGGCTACCGTCACCCAGCAATTGTTGAAAACGAGGAGTATCCTGAGGTGTACTGATAATCAAAATATCGCGAAt                                                                                          <  2:613459/235‑1 (MQ=255)
                                                                                                                              ggCGGCCTACATTAGCTGGGGCAGATCGTGACCGTAAATGATATAATCACCAAGAACCAAAGCACAATCATCACCACCAATAAACTCTTCACCGATGATCAAGGCCTGCGCGAGGCCATCTGGGCTAGGTTGCCCTTTGTACTGAAGATTCAGGCCCCACTGGCTACCGTCACCCGGCAATTGTTGAAAACGAGGAGTATCCTGAGGTGTACGGATAATCAAAATATCGCGAATAc                                                                                        <  2:163456/235‑1 (MQ=255)
                                                                                                                               gCGGCCTCCATTAGCTTCGGCAGATCGTGACCGTAAAAGATATTATCACCAAGAACCAAAGCACAATCATCACCACCAATAAACTCTTCACCGATGATCAATGCCTGCGCGAGGCCATCTGGGCTAGGTTGCACTTTGTACTGAAGATTCAGGCCCCACTGGCTACCGTCACCCAGCAATTGTTGAAAACGAGGAGTATCCTGAGGGGTACTGATAATCAAAATATCTCGAATaa                                                                                        >  2:265377/1‑234 (MQ=255)
                                                                                                                                   ccTCCATTAGCTTCGGCAGATCGTGACCGTAAAAGATATTATCACCAAGAACCAAAGCACAATCATCACCACCAATAAACTCTTCACCGATGATCAATGCCTGCGCGAGGCCATCTGGGCTAGGTTGCACTTTGTACTGAAGATTCAGGCCCCACTGGCTACCGTCACCCAGCAATTGTTGAAAACGAGGAGTATCCTGAGGTGTACTGATAATCAAAATATCGCGAATACCCGcc                                                                                   >  2:419409/1‑236 (MQ=255)
                                                                                                                                   ccTCCATTAGCTTCGGCAGATCGTGACCGTAAAAGATATTATCACCAAGAACCAAAGCACAATCATCACCACCAATAAACTCTTCACCGATGATCAATGCCTGCGCGAGGCCATCTGGGCTAGGTTGCACTTTGTACTGAAGATTCAGGCCCCACTGGCTACCGTCACCCAGCAATTGTTGAAAACGAGGAGTAGCCTTAGGTGTACTGATAATCAAAATATCGCGAATAccacac                                                                                   >  2:30176/1‑232 (MQ=255)
                                                                                                                                     tCCATTAGCTTCGGCAGATCGTGACCGTAAAAGATATTATCACCAAGAACCAAAGCACAATCATCACCACCAATAAACTCTTCACCGATGATCAATGCCTGCGCGAGGCCATCTGGGCTAGGTTGCACTTTGTACTGAAGATTCAGGCCCCACTGGCTACCGTCACCCAGCAATTGTTGAAAACGAGGAGTATCCTGAGGTGTACTGATAATCAAAATATCGCGAATACCCGCCaa                                                                                 <  2:592172/236‑1 (MQ=255)
                                                                                                                                      ccATTAGCTTCGGCAGATCGTGACCGTAAAAGATATTATCACCAAGAACCAAAGCACAATCATCACCACCAATAAACTCTTCACCGATGATCAATGCCTGCGCGAGGCCATCTGGGCTAGGTTGCACTTTGTACTGAAGATTCAGGCCCCACTGGCTACCGTCACCCAGCAATTGTTGAAAACGAGGAGTATCCTGAGGTGTACTGATAATCAAAATATCGCGAATACCCGCCaa                                                                                 <  1:465704/235‑1 (MQ=255)
                                                                                                                                                   aaGATCGTGACCGTAAAAGATATTATCACCAAGAACCAAAGCACAATCATCACCACCAATAAACTCTTCACCGATGATCAATGCCTGCGCGAGGCCATCTGGGCTAGGTTGCACTTTGTACTGAAGATTCAGGCCCCACTGGCTACCGTCACCCAGCAATTGTTGAAAACGAGGAGTATCCTGAGGTGTACTGATAATCAAAATATCGCGAATACCCGCCAACATCAGTGTagaga                                                                   <  1:647743/235‑1 (MQ=255)
                                                                                                                                                    aGATCGTGACCGTAAAAGATATTATCACCAAGAACCAAAGCACAATCATCACCACCAATAAACTCTTCACCGATGATCAATGCCTGCGCGAGGCCATCTGGGCTAGGTTGCACTTTGTACTGAAGATTCAGGCCCCACTGGCTACCGTCACCCAGCAATTGTTGAAAACGAGGAGTATCCTGAGGTGTACTGATAATCAAAATATCGCGAATACCCGCCAACATCAGTGTagagag                                                                  <  1:419409/236‑1 (MQ=255)
                                                                                                                                                    aGATCGTGACCGTAAAAGATATTATCACCAAGAACCAAAGCACAATCATCACCACCAATAAACTCTTCACCGATGATCAATGCCTGCGCGAGGCCATCTGGGCTAGGTTGCACTTTGTACTGAAGATTCAGGCCCCACTGGCTACCGTCACCCAGCAATTGTTGAAAACGAGGAGTATCCTGAGGTGTACTGATAATCAAAATATCGCGAATACCCGCCAACATCAGTGTagagag                                                                  <  1:673295/236‑1 (MQ=255)
                                                                                                                                                    aGATCGTGACCGTAAAAGATATTATCACCAAGAACCAAAGCACAATCATCACCACCAATAAACTCTTCACCGATGATCAATGCCTGCGCGAGCCCATCTGGGCTAGGTTGCACTTTGTACTGAAGATTCAGGCCCCACTGGCTACCGTCACCCAGCAATTGTTGAAAACGAGGAGTATCCTGAGGTGTACTGATAATCAAAATATCGCGAATACCCGCCAACATCAGTGTagagag                                                                  <  1:685180/236‑1 (MQ=255)
                                                                                                                                                        cGTGACCGTAAAAGATATTATCACCAAGAACCAAAGCACAATCATCACCACCAATAAACTCTTCACCGATGATCAATGCCTGCGCGAGGCCATCTGGGCTAGGTTGCACTTTGTACTGAAGATTCAGGcccc                                                                                                                                                                      >  2:230581/1‑132 (MQ=255)
                                                                                                                                                        cGTGACCGTAAAAGATATTATCACCAAGAACCAAAGCACAATCATCACCACCAATAAACTCTTCACCGATGATCAATGCCTGCGCGAGGCCATCTGGGCTAGGTTGCACTTTGTACTGAAGATTCAGGcccc                                                                                                                                                                      <  1:230581/132‑1 (MQ=255)
                                                                                                                                                                       tattatCACCAAGAACCAAAGCACAATCATCACCACCAATAAACTCTTCACCGATGATCAATGCCTGCGCGAGGCCATCTGGGCTAGGTTGCACTTTGTACTGAAGATTCAGGCCCCACTGGCTACCGTCACCCAGCAATTGTTGAAAACGAGGAGTATCCTGAGGTGTACTGATAATCAAAATATCGCGAATACCCGCCAACATCAGTGTAGAGAGCGGGTAATAGATCATCGGt                                               <  2:486885/236‑1 (MQ=255)
                                                                                                                                                                        attatCACCAAGAACCAAAGCACAATCATCACCACCAATAAACTCTTCACCGATGATCAATGCCTGCGCGAGGCCATCTGGGCTAGGTTGCACTTTGTACTGAAGATTCAGGCCCCACTGGCTACCGTCACCCAGCAATTGTTGAAAACGAGGAGTATCCTGAGGTGTACTGATAATCAAAATATCGCGAATACCCGCCAACATCAGTGTAGAGAGCGGGTAATAGATCATCGGt                                               >  1:173352/1‑235 (MQ=255)
                                                                                                                                                                                 aaGAACCAAAGCAAAATCATCACCACCAATAATCCCTTCACCGATGATCAATGCATGCGCGAGGCCATCTGGGCTAGGTTGCACTTTGTACTGAAGATTCAGGCCCCACTGGCTACCGTCACCCAGCAATTGTTGAAAACGAGGAGTATCCTGAGGTGTACTGATAATCAAAATATCGCGAATACCCGCCAACATCAGTGTAGAGAGCGGGTAATAGATCATCGGTTTATCATaaa                                     <  2:444096/236‑1 (MQ=255)
                                                                                                                                                                                              caATCATCACCACCAATAAACTCTTCACCGATGATCAATGCCTGCGCGAGGCCATCTGGGCTAGGTTGCACTTTGTACTGAAGATTCAGGCCCCACTGGCTACCGTCACCCAGCAATTGTTGAAAACGAGGAGTATCCTGAGGTGTACTGATAATCAAAATATCGCGAATACCCGCCAACATCAGTGTAGAGAGCGGGTAATAGATCATCGGTTTATCATAAATAGGTa                               >  1:160829/1‑229 (MQ=255)
                                                                                                                                                                                                      accaccAATAAACTCTTCACCGATGATCAATGCCTGCGCGAGGCCATCTGGGCTAGGTTGCACTTTGTACTg                                                                                                                                                                                    >  1:217998/1‑72 (MQ=255)
                                                                                                                                                                                                      accaccAATAAACTCTTCACCGATGATCAATGCCTGCGCGAGGCCATCTGGGCTAGGTTGCACTTTGTACTg                                                                                                                                                                                    <  2:217998/72‑1 (MQ=255)
                                                                                                                                                                                                           caATAAACTCTTCACCGATGATCAATGCCTGCGCGAGGCCATCTGGGCTAGGTTGCACTTTGTACTGAAGATTCAGGCCCCACTGGCTACCGTCACCCAGCAATTGTTGAAAACGAGGAGTATCCTGAGGTGTACTGATAATCAAAATATCGCGAATACCCGCCAACATCAGTGTAGAGAGCGGGTAATAGATCATCGGTTTATCATAAATAGGTAATAGCTGTTTACTGACAGcc           >  1:419886/1‑236 (MQ=255)
                                                                                                                                                                                                                  ctctTCACCGATGATCAATGCCTGCGCGAGGCCATCTGGGATAGGTTGCACTTTGTACTGAAGATTCAGGCCCCACTGGCTACCGTCACCCAGCAATTGTTGAAAACGAGGAGTATCCTGAGGTGTACTGATAATCAAAATATCGCGAATACCCGCCAACATCAGTGTAGAGAGCGGGTAATAGATCATCGGTTTATCATAAATAGGTAATAGCTGTTTACTGACAGCCATAGTca    <  1:265377/236‑1 (MQ=255)
                                                                                                                                                                                                                  ctctGCACGGATGATCAATCCCTGCACGAGTCCATCGGGTCTAGGTTGCTATTTGTACTGAAGATTCAGGCCCCACTGGCTACCGTCACCCAGCAATTGTTAAAAACGAGGAGTATCCTGAGGTGTACTGATAATCAAAATATCGCGAATCCCCGCCAACATCAGTGTAGAGAGCGGGTAATAGATCATCGGTTTATCATAAATAGGTAATAGCTGTTTACTGACAGCCATAGTca    <  2:173352/236‑1 (MQ=255)
                                                                                                                                                                                                                    ctTCACCGATGATCAATGCCTGCGCGAGGCCATCTGGGCTAGGTTGCACTTTGTACTGAAGATTCAGGCCCCACTGGCTACCGTCACCCAGCAATTGTTGAAAACGAGGAGTATCCTGAGGTGTACTGATAATCAAAATATCGCGAATACCCGCCAACATCAGTGTAGAGAGCGGGTAATAGATCATCGGTTTATCATAAATAGGTAATAGCTGTTTACTGACAGCCATAGTcaca  >  1:599019/1‑236 (MQ=255)
                                                                                                                                                                                                                      tCACCGATGATCAATGCCTGCGCGAGGCCATCTGGGCTAGGTTGCACTTTGTACTGAAGATTCAGGCCCCACTGGCTACCGTCACCCAGCAATTGTTGAAAACGAGGAGTATCCTGAGGTGTACTGATAATCAAAATATCGCGAATACCCGCCAACa                                                                               >  1:663959/1‑157 (MQ=255)
                                                                                                                                                                                                                      tCACCGATGATCAATGCCTGCGCGAGGCCATCTGGGCTAGGTTGCACTTTGTACTGAAGATTCAGGCCCCACTGGCTACCGTCACCCAGCAATTGTTGAAAACGAGGAGTATCCTGAGGTGTACTGATAATCAAAATATCGCGAATACCCGCCAACa                                                                               <  2:663959/157‑1 (MQ=255)
                                                                                                                                                                                                                                 |                                                                                                                                                                                                                              
CTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCAAACTCAACGACACCATAGCGTTCTGGATCATTAACGTGATAGGCAAATACCGTTGCACCACTTTCTTTGTTAACAGCGGCCTCCATTAGCTTCGGCAGATCGTGACCGTAAAAGATATTATCACCAAGAACCAAAGCACAATCATCACCACCAATAAACTCTTCACCGATGATAAATGCCTGCGCGAGGCCATCTGGGCTAGGTTGCACTTTGTACTGAAGATTCAGGCCCCACTGGCTACCGTCACCCAGCAATTGTTGAAAACGAGGAGTATCCTGAGGTGTACTGATAATCAAAATATCGCGAATACCCGCCAACATCAGTGTAGAGAGCGGGTAATAGATCATCGGTTTATCATAAATAGGTAATAGCTGTTTACTGACAGCCATAGTCACA  >  NC_000913/2110516‑2110963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A